(Linear) (Six-base) MAPSORT of: KIEE5775.seq Check: 21 from: 1 to: 1872 KIEE5775.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 106 1872 Size: 106 1766 Acc65I G'GTAC_C Cuts at: 0 817 1872 Size: 817 1055 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 606 805 1228 1264 1306 1872 Size: 606 199 423 36 42 566 Fragments arranged by size: 606 566 423 199 42 36 AflIII A'CryG_T Cuts at: 0 479 1872 Size: 479 1393 AhdI GACnn_n'nnGTC Cuts at: 0 852 1872 Size: 852 1020 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 458 1872 Size: 458 1414 AlwNI CAG_nnn'CTG Cuts at: 0 159 1757 1872 Size: 159 1598 115 Fragments arranged by size: 1598 159 115 ApaLI G'TGCA_C Cuts at: 0 519 1872 Size: 519 1353 ApoI r'AATT_y Cuts at: 0 36 238 248 316 894 1312 1548 1872 Size: 36 202 10 68 578 418 236 324 Fragments arranged by size: 578 418 324 236 202 68 36 10 AvaI C'yCGr_G Cuts at: 0 125 1676 1872 Size: 125 1551 196 Fragments arranged by size: 1551 196 125 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 809 842 1872 Size: 809 33 1030 Fragments arranged by size: 1030 809 33 BamHI G'GATC_C Cuts at: 0 129 1872 Size: 129 1743 BanI G'GyrC_C Cuts at: 0 11 817 1872 Size: 11 806 1055 Fragments arranged by size: 1055 806 11 BanII G_rGCy'C Cuts at: 0 467 1291 1872 Size: 467 824 581 Fragments arranged by size: 824 581 467 BbeI G_GCGC'C Cuts at: 0 15 1872 Size: 15 1857 BbsI GAAGACnn'nnnn_ Cuts at: 0 941 1213 1363 1872 Size: 941 272 150 509 Fragments arranged by size: 941 509 272 150 BciVI GTATCCnnnnn_n' Cuts at: 0 494 1604 1872 Size: 494 1110 268 Fragments arranged by size: 1110 494 268 BclI T'GATC_A Cuts at: 0 243 1872 Size: 243 1629 BglI GCCn_nnn'nGGC Cuts at: 0 121 1872 Size: 121 1751 BglII A'GATC_T Cuts at: 0 1031 1872 Size: 1031 841 BlpI GC'TnA_GC Cuts at: 0 1297 1872 Size: 1297 575 Bme1580I G_kGCm'C Cuts at: 0 523 1872 Size: 523 1349 BmgBI CAC'GTC Cuts at: 0 480 1872 Size: 480 1392 BmrI ACTGGGnnnn_n' Cuts at: 0 643 1821 1872 Size: 643 1178 51 Fragments arranged by size: 1178 643 51 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1530 1872 Size: 88 1442 342 Fragments arranged by size: 1442 342 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 955 1872 Size: 955 917 BsaAI yAC'GTr Cuts at: 0 366 1872 Size: 366 1506 BsaHI Gr'CG_yC Cuts at: 0 12 1872 Size: 12 1860 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1034 1064 1872 Size: 1034 30 808 Fragments arranged by size: 1034 808 30 BseYI C'CCAG_C Cuts at: 0 203 1799 1872 Size: 203 1596 73 Fragments arranged by size: 1596 203 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 211 353 1090 1872 Size: 183 28 142 737 782 Fragments arranged by size: 782 737 183 142 28 BsiEI CG_ry'CG Cuts at: 0 116 1872 Size: 116 1756 BsiHKAI G_wGCw'C Cuts at: 0 82 523 1291 1872 Size: 82 441 768 581 Fragments arranged by size: 768 581 441 82 BsmI GAATG_Cn' Cuts at: 0 1250 1872 Size: 1250 622 Bsp1286I G_dGCh'C Cuts at: 0 82 467 523 1291 1872 Size: 82 385 56 768 581 Fragments arranged by size: 768 581 385 82 56 BspHI T'CATG_A Cuts at: 0 1170 1872 Size: 1170 702 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 106 1872 Size: 106 1766 BsrBI CCG'CTC Cuts at: 0 55 1872 Size: 55 1817 BsrDI GCAATG_nn' Cuts at: 0 746 1276 1872 Size: 746 530 596 Fragments arranged by size: 746 596 530 BssSI C'ACGA_G Cuts at: 0 229 1872 Size: 229 1643 BstAPI GCAn_nnn'nTGC Cuts at: 0 159 1872 Size: 159 1713 BstEII G'GTnAC_C Cuts at: 0 637 1872 Size: 637 1235 BstXI CCAn_nnnn'nTGG Cuts at: 0 140 1872 Size: 140 1732 BstYI r'GATC_y Cuts at: 0 129 1031 1389 1872 Size: 129 902 358 483 Fragments arranged by size: 902 483 358 129 BtgI C'CryG_G Cuts at: 0 446 1872 Size: 446 1426 BtsI GCAGTG_nn' Cuts at: 0 160 1228 1688 1872 Size: 160 1068 460 184 Fragments arranged by size: 1068 460 184 160 ClaI AT'CG_AT Cuts at: 0 176 1872 Size: 176 1696 DraI TTT'AAA Cuts at: 0 1578 1872 Size: 1578 294 DraIII CAC_nnn'GTG Cuts at: 0 1671 1872 Size: 1671 201 EaeI y'GGCC_r Cuts at: 0 113 1020 1872 Size: 113 907 852 Fragments arranged by size: 907 852 113 EagI C'GGCC_G Cuts at: 0 113 1872 Size: 113 1759 EarI CTCTTCn'nnn_ Cuts at: 0 978 1872 Size: 978 894 EcoICRI GAG'CTC Cuts at: 0 1289 1872 Size: 1289 583 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 606 805 1228 1264 1306 1530 1872 Size: 88 518 199 423 36 42 224 342 Fragments arranged by size: 518 423 342 224 199 88 42 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 599 1872 Size: 599 1273 EcoO109I rG'GnC_Cy Cuts at: 0 1791 1872 Size: 1791 81 HaeII r_GCGC'y Cuts at: 0 15 111 1872 Size: 15 96 1761 Fragments arranged by size: 1761 96 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 838 870 1034 1066 1078 1110 1872 Size: 838 32 164 32 12 32 762 Fragments arranged by size: 838 762 164 32 32 32 12 HindIII A'AGCT_T Cuts at: 0 1001 1872 Size: 1001 871 KasI G'GCGC_C Cuts at: 0 11 1872 Size: 11 1861 KpnI G_GTAC'C Cuts at: 0 821 1872 Size: 821 1051 MscI TGG'CCA Cuts at: 0 1022 1872 Size: 1022 850 MslI CAynn'nnrTG Cuts at: 0 711 1856 1872 Size: 711 1145 16 Fragments arranged by size: 1145 711 16 MspA1I CmG'CkG Cuts at: 0 207 494 859 1872 Size: 207 287 365 1013 Fragments arranged by size: 1013 365 287 207 NarI GG'CG_CC Cuts at: 0 12 1872 Size: 12 1860 NcoI C'CATG_G Cuts at: 0 446 1872 Size: 446 1426 NspI r_CATG'y Cuts at: 0 1063 1872 Size: 1063 809 PasI CC'CwG_GG Cuts at: 0 456 1872 Size: 456 1416 PfoI T'CCnGG_A Cuts at: 0 698 978 1872 Size: 698 280 894 Fragments arranged by size: 894 698 280 PmlI CAC'GTG Cuts at: 0 366 1872 Size: 366 1506 PstI C_TGCA'G Cuts at: 0 155 1872 Size: 155 1717 PvuII CAG'CTG Cuts at: 0 207 494 859 1872 Size: 207 287 365 1013 Fragments arranged by size: 1013 365 287 207 SacI G_AGCT'C Cuts at: 0 1291 1872 Size: 1291 581 SfcI C'TryA_G Cuts at: 0 151 1761 1872 Size: 151 1610 111 Fragments arranged by size: 1610 151 111 SfoI GGC'GCC Cuts at: 0 13 1872 Size: 13 1859 SmlI C'TyrA_G Cuts at: 0 970 1872 Size: 970 902 SpeI A'CTAG_T Cuts at: 0 1423 1872 Size: 1423 449 StyI C'CwwG_G Cuts at: 0 446 1188 1872 Size: 446 742 684 Fragments arranged by size: 742 684 446 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 217 1813 1872 Size: 217 1596 59 Fragments arranged by size: 1596 217 59 TatI w'GTAC_w Cuts at: 0 1381 1872 Size: 1381 491 XbaI T'CTAG_A Cuts at: 0 1254 1872 Size: 1254 618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 453 1872 Size: 453 1419 XmnI GAAnn'nnTTC Cuts at: 0 938 1872 Size: 938 934 Enzymes that do cut: AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I NarI NcoI NspI PasI PfoI PmlI PstI PvuII SacI SfcI SfoI SmlI SpeI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BbvCI BcgI BfrBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BseRI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I XhoI XmaI ZraI