################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MluI MluNI MroI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp45I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 561 CGCG CG^CG AlwNI 589 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 268 ATTAAT AT^TAAT AsnI 268 ATTAAT AT^TAAT Asp700I 3 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 761 GAGCTC G[AT]GC[AT]^C BanII 761 GAGCTC G[GA]GC[CT]^C BcnI 136 CCGGG CC^[GC]GG BfaI 146 CTAG C^TAG BlpI 583 GCTCAGC GC^T[ACGT]AGC Bpu1102I 583 GCTCAGC GC^T[ACGT]AGC BsiHKAI 761 GAGCTC G[AT]GC[AT]^C BsmAI 810 GTCTC GTCTC(1/5) Bsp1407I 542 TGTACA T^GTACA BspMI 588 GCAGGT ACCTGC(4/8) BsrGI 542 TGTACA T^GTACA BstUI 561 CGCG CG^CG BstYI 116 GGATCT [GA]^GATC[CT] Cac8I 310 GCTTGC GC[ACGT]^[ACGT]GC CelII 583 GCTCAGC GC^T[ACGT]AGC DsaI 878 CCATGG C^C[GA][CT]GG EcoT22I 841 ATGCAT ATGCA^T HaeIII 375 GGCC GG^CC HapII 136 CCGG C^CGG HgaI 223 GACGC GACGC(5/10) HpaII 136 CCGG C^CGG MaeI 146 CTAG C^TAG MaeII 278 ACGT A^CGT MflI 116 GGATCT [GA]^GATC[CT] MspI 136 CCGG C^CGG MvnI 561 CGCG CG^CG NciI 136 CCGGG CC^[GC]GG NcoI 878 CCATGG C^CATGG NsiI 841 ATGCAT ATGCA^T PleI 601 GACTC GAGTC(4/5) PshBI 268 ATTAAT AT^TAAT PstI 940 CTGCAG CTGCA^G SacI 761 GAGCTC GAGCT^C SspBI 542 TGTACA T^GTACA StuI 374 AGGCCT AGG^CCT XbaI 145 TCTAGA T^CTAGA XhoII 116 GGATCT [GA]^GATC[CT] XmnI 3 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvaII 591 GGTCC G^G[AT]CC 873 GGACC BmyI 761 GAGCTC G[AGT]GC[ACT]^C 963 GTGCCC BsiYI 286 CCACAGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 948 CCCAGACATGG BslI 286 CCACAGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 948 CCCAGACATGG BsmFI 528 GGGAC GGGAC(10/14) 537 GGGAC Bsp1286I 761 GAGCTC G[AGT]GC[ACT]^C 963 GTGCCC BstNI 27 CCAGG CC^[AT]GG 735 CCTGG CfoI 63 GCGC GCG^C 958 GCGC Cfr13I 591 GGTCC G^G[ACGT]CC 873 GGACC DraII 590 AGGTCCT [GA]G^G[ACGT]CC[CT] 872 AGGACCC EcoO109I 590 AGGTCCT [GA]G^G[ACGT]CC[CT] 872 AGGACCC EcoRII 27 CCAGG ^CC[AT]GG 735 CCTGG EcoRV 247 GATATC GAT^ATC 625 GATATC EcoT14I 492 CCAAGG C^C[AT][AT]GG 878 CCATGG HaeII 62 GGCGCT [GA]GCGC^[CT] 957 GGCGCT HhaI 63 GCGC GCG^C 958 GCGC HinP1I 63 GCGC G^CGC 958 GCGC HphI 496 GGTGA GGTGA(8/7) 216 TCACC MfeI 692 CAATTG C^AATTG 710 CAATTG MunI 692 CAATTG C^AATTG 710 CAATTG MvaI 27 CCAGG CC^[AT]GG 735 CCTGG NlaIV 338 GGAACC GG[ACGT]^[ACGT]CC 873 GGACCC PpuMI 590 AGGTCCT [GA]G^G[AT]CC[CT] 872 AGGACCC PvuII 289 CAGCTG CAG^CTG 937 CAGCTG Sau96I 591 GGTCC G^G[ACGT]CC 873 GGACC SfcI 239 CTATAG C^T[GA][CT]AG 940 CTGCAG StyI 492 CCAAGG C^C[AT][AT]GG 878 CCATGG XcmI 440 CCAATACTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 878 CCATGGACATAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BsaJI BsrI BssKI BstF5I DdeI DpnII EarI Fnu4HI FokI HinfI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I