################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 174 GTAC GT^AC AflII 69 CTTAAG C^TTAAG AluI 597 AGCT AG^CT AocI 30 CCTGAGG CC^T[ACGT]AGG AseI 238 ATTAAT AT^TAAT AsnI 238 ATTAAT AT^TAAT AspHI 562 GAGCAC G[AT]GC[AT]^C AvaII 414 GGACC G^G[AT]CC BalI 623 TGGCCA TGG^CCA BanII 199 GAGCCC G[GA]GC[CT]^C BbvI 422 GCAGC GCAGC(8/12) BfrI 69 CTTAAG C^TTAAG BsiHKAI 562 GAGCAC G[AT]GC[AT]^C BsiYI 154 CCCACCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 154 CCCACCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 376 GCAATG GCAATG(2/0) Bsu36I 30 CCTGAGG CC^T[ACGT]AGG Cac8I 54 GCAAGC GC[ACGT]^[ACGT]GC DpnII 474 GATC ^GATC DraI 270 TTTAAA TTT^AAA DsaI 159 CCACGG C^C[GA][CT]GG EaeI 623 TGGCCA [CT]^GGCC[GA] EarI 196 GAAGAG CTCTTC(1/4) Eco81I 30 CCTGAGG CC^T[ACGT]AGG EcoRI 116 GAATTC G^AATTC Fnu4HI 422 GCAGC GC^[ACGT]GC ItaI 422 GCAGC GC^[ACGT]GC Ksp632I 196 GAAGAG CTCTTC(1/4) MaeII 493 ACGT A^CGT MboI 474 GATC ^GATC MluNI 623 TGGCCA TGG^CCA MscI 623 TGGCCA TGG^CCA MslI 393 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 477 CAGCGG C[AC]G^C[GT]G MwoI 490 GCTACGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 290 CATATG CA^TATG NdeII 474 GATC ^GATC NlaIII 457 CATG CATG^ NlaIV 7 GGAACC GG[ACGT]^[ACGT]CC PacI 184 TTAATTAA TTAAT^TAA PshBI 238 ATTAAT AT^TAAT RsaI 174 GTAC GT^AC SapI 196 GAAGAGC GCTCTTC(1/4) Sau3AI 474 GATC ^GATC SfcI 107 CTGTAG C^T[GA][CT]AG SmlI 69 CTTAAG C^T[CT][GA]AG SspI 274 AATATT AAT^ATT TfiI 590 GATTC G^A[AT]TC TseI 422 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 5 GCGG CCGC(-3/-1) 479 GCGG AcsI 116 GAATTC [GA]^AATT[CT] 503 AAATTT ApoI 116 GAATTC [GA]^AATT[CT] 503 AAATTT BbsI 574 GAAGAC GAAGAC(2/6) 530 GTCTTC BmyI 199 GAGCCC G[AGT]GC[ACT]^C 562 GAGCAC BpuAI 574 GAAGAC GAAGAC(2/6) 530 GTCTTC BsmFI 247 GGGAC GGGAC(10/14) 585 GGGAC Bsp1286I 199 GAGCCC G[AGT]GC[ACT]^C 562 GAGCAC BsrI 609 ACTGG ACTGG(1/-1) 321 CCAGT BssKI 303 CCTGG ^CC[ACGT]GG 435 CCTGG BstNI 303 CCTGG CC^[AT]GG 435 CCTGG BstXI 159 CCACGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 236 CCATTAATTTGG Cfr13I 137 GGCCC G^G[ACGT]CC 414 GGACC EcoRII 303 CCTGG ^CC[AT]GG 435 CCTGG EcoT14I 11 CCTTGG C^C[AT][AT]GG 417 CCAAGG HaeIII 137 GGCC GG^CC 624 GGCC MvaI 303 CCTGG CC^[AT]GG 435 CCTGG PleI 439 GACTC GAGTC(4/5) 542 GAGTC Sau96I 137 GGCCC G^G[ACGT]CC 414 GGACC ScrFI 303 CCTGG CC^[ACGT]GG 435 CCTGG StyI 11 CCTTGG C^C[AT][AT]GG 417 CCAAGG TaqI 362 TCGA T^CGA 600 TCGA TthHB8I 362 TCGA T^CGA 600 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BsaJI DdeI HinfI MboII MnlI MseI Tru9I Tsp509I TspRI