(Circular) (Six-base) MAPSORT of: pFN21AE2466.seq Check: 5686 from: 1 to: 7663 pFN21AE2466 7663 bp With 182 enzymes: SgfI * August 29, 2014 11:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5473 278 Size: 53 83 186 936 3937 2468 Fragments arranged by size: 3937 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5006 1937 Size: 3069 4594 Acc65I G'GTAC_C Cuts at: 4984 4984 Size: 7663 AclI AA'CG_TT Cuts at: 1563 5790 6163 1563 Size: 4227 373 3063 Fragments arranged by size: 4227 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2152 2546 3425 3863 4103 4109 5726 7110 Size: 520 394 879 438 240 6 1617 1384 Cuts at: 7110 7378 1632 Size: 268 1917 Fragments arranged by size: 1917 1617 1384 879 520 438 394 268 240 6 AfeI AGC'GCT Cuts at: 2378 2378 Size: 7663 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7466 AflIII A'CryG_T Cuts at: 1203 2777 3921 4461 6562 7539 1203 Size: 1574 1144 540 2101 977 1327 Fragments arranged by size: 2101 1574 1327 1144 977 540 AgeI A'CCGG_T Cuts at: 6494 6494 Size: 7663 AhdI GACnn_n'nnGTC Cuts at: 6392 6392 Size: 7663 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7663 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4022 1184 Size: 2838 4825 AlwNI CAG_nnn'CTG Cuts at: 3168 4702 4834 4837 5406 6978 3168 Size: 1534 132 3 569 1572 3853 Fragments arranged by size: 3853 1572 1534 569 132 3 ApaLI G'TGCA_C Cuts at: 5720 6876 5720 Size: 1156 6507 ApoI r'AATT_y Cuts at: 1493 3853 4972 5198 5350 7459 1493 Size: 2360 1119 226 152 2109 1697 Fragments arranged by size: 2360 2109 1697 1119 226 152 AseI AT'TA_AT Cuts at: 160 1032 2556 6217 160 Size: 872 1524 3661 1606 Fragments arranged by size: 3661 1606 1524 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7663 AvaI C'yCGr_G Cuts at: 1870 1943 2224 2679 3910 4988 1870 Size: 73 281 455 1231 1078 4545 Fragments arranged by size: 4545 1231 1078 455 281 73 BamHI G'GATC_C Cuts at: 4993 5360 4993 Size: 367 7296 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4984 6439 618 Size: 325 217 627 3197 1455 1842 Fragments arranged by size: 3197 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2281 2349 3182 4103 4167 4320 4982 1391 Size: 890 68 833 921 64 153 662 4072 Fragments arranged by size: 4072 921 890 833 662 153 68 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3052 3226 3238 4684 928 Size: 971 1153 174 12 1446 3907 Fragments arranged by size: 3907 1446 1153 971 174 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5854 5888 1903 Size: 34 3917 34 3678 Fragments arranged by size: 3917 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5555 6765 5555 Size: 1210 6453 BclI T'GATC_A Cuts at: 1548 4200 1548 Size: 2652 5011 BfrBI ATG'CAT Cuts at: 3987 3987 Size: 7663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3260 3647 4664 Size: 107 122 71 631 605 1588 387 1017 Cuts at: 4664 4676 6274 136 Size: 12 1598 1525 Fragments arranged by size: 1598 1588 1525 1017 631 605 387 122 107 71 12 BglII A'GATC_T Cuts at: 2760 4584 7658 2760 Size: 1824 3074 2765 Fragments arranged by size: 3074 2765 1824 BlpI GC'TnA_GC Cuts at: 2634 3181 3995 5080 2634 Size: 547 814 1085 5217 Fragments arranged by size: 5217 1085 814 547 Bme1580I G_kGCm'C Cuts at: 1790 2111 2923 3143 5724 6880 1790 Size: 321 812 220 2581 1156 2573 Fragments arranged by size: 2581 2573 1156 812 321 220 BmgBI CAC'GTC Cuts at: 1318 2778 4428 1318 Size: 1460 1650 4553 Fragments arranged by size: 4553 1650 1460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4741 5394 6352 Size: 416 53 83 399 24 3324 653 958 Cuts at: 6352 442 Size: 1753 Fragments arranged by size: 3324 1753 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7663 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 7663 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 2444 2450 2582 4181 6323 1239 Size: 1193 12 6 132 1599 2142 2579 Fragments arranged by size: 2579 2142 1599 1193 132 12 6 Bpu10I CC'TnA_GC Cuts at: 2338 2338 Size: 7663 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5788 6653 6951 5788 Size: 865 298 6500 Fragments arranged by size: 6500 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 3571 6326 882 Size: 617 1056 1016 2755 2219 Fragments arranged by size: 2755 2219 1056 1016 617 BsaAI yAC'GTr Cuts at: 493 1204 3877 3922 5454 493 Size: 711 2673 45 1532 2702 Fragments arranged by size: 2702 2673 1532 711 45 BsaBI GATnn'nnATC Cuts at: 3029 4049 7657 3029 Size: 1020 3608 3035 Fragments arranged by size: 3608 3035 1020 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2321 3612 3907 4296 Size: 53 83 186 936 788 1291 295 389 Cuts at: 4296 4956 5470 5852 6487 275 Size: 660 514 382 635 1451 Fragments arranged by size: 1451 1291 936 788 660 635 514 389 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 2232 6096 6494 6768 6915 7326 2232 Size: 3864 398 274 147 411 2569 Fragments arranged by size: 3864 2569 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3551 3581 3614 3644 4493 Size: 30 165 30 2148 30 33 30 849 Cuts at: 4493 4523 4625 4655 1178 Size: 30 102 30 4186 Fragments arranged by size: 4186 2148 849 165 102 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 3359 3464 3649 3827 4130 4501 4829 Size: 1219 954 105 185 178 303 371 328 Cuts at: 4829 1186 Size: 4020 Fragments arranged by size: 4020 1219 954 371 328 303 185 178 105 BseYI C'CCAG_C Cuts at: 3007 3098 3315 3544 4009 4927 4934 6866 7362 Size: 91 217 229 465 918 7 1932 496 Cuts at: 7362 3007 Size: 3308 Fragments arranged by size: 3308 1932 918 496 465 229 217 91 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 2521 2837 4601 1163 Size: 573 537 248 316 1764 4225 Fragments arranged by size: 4225 1764 573 537 316 248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2179 5113 5874 6902 1540 Size: 275 186 39 139 2934 761 1028 2301 Fragments arranged by size: 2934 2301 1028 761 275 186 139 39 BsiHKAI G_wGCw'C Cuts at: 2598 2640 2747 3182 3282 4320 4791 5724 5809 Size: 42 107 435 100 1038 471 933 85 Cuts at: 5809 6880 2598 Size: 1071 3381 Fragments arranged by size: 3381 1071 1038 933 471 435 107 100 85 42 BsmI GAATG_Cn' Cuts at: 5174 5267 5174 Size: 93 7570 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2281 2349 2598 2640 2747 2923 Size: 399 321 170 68 249 42 107 176 Cuts at: 2923 3143 3182 3282 4103 4167 4320 4791 4982 Size: 220 39 100 821 64 153 471 191 Cuts at: 4982 5724 5809 6880 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 821 742 471 399 321 249 220 191 176 170 153 107 100 85 68 64 42 39 BspEI T'CCGG_A Cuts at: 7326 7326 Size: 7663 BspHI T'CATG_A Cuts at: 3579 4713 5552 7282 3579 Size: 1134 839 1730 3960 Fragments arranged by size: 3960 1730 1134 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 3167 4581 5018 844 Size: 1259 1064 1414 437 3489 Fragments arranged by size: 3489 1414 1259 1064 437 BsrBI CCG'CTC Cuts at: 1394 1823 2434 4861 5550 1394 Size: 429 611 2427 689 3507 Fragments arranged by size: 3507 2427 689 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6158 6332 65 Size: 1183 4910 174 1396 Fragments arranged by size: 4910 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 3435 3894 4327 6307 6494 1700 Size: 110 368 1257 459 433 1980 187 2869 Fragments arranged by size: 2869 1980 1257 459 433 368 187 110 BsrGI T'GTAC_A Cuts at: 96 2780 3015 3924 4464 96 Size: 2684 235 909 540 3295 Fragments arranged by size: 3295 2684 909 540 235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7663 BssSI C'ACGA_G Cuts at: 2363 2809 4112 5723 6735 2363 Size: 446 1303 1611 1012 3291 Fragments arranged by size: 3291 1611 1303 1012 446 BstAPI GCAn_nnn'nTGC Cuts at: 794 4834 794 Size: 4040 3623 BstBI TT'CG_AA Cuts at: 6467 6467 Size: 7663 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7663 BstXI CCAn_nnnn'nTGG Cuts at: 2959 3274 3391 2959 Size: 315 117 7231 Fragments arranged by size: 7231 315 117 BstYI r'GATC_y Cuts at: 1972 2611 2760 4284 4584 4770 4993 5360 5745 Size: 639 149 1524 300 186 223 367 385 Cuts at: 5745 5762 7214 7300 7312 7658 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1524 1452 639 385 367 346 300 223 186 149 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2455 2566 3081 3192 3228 3489 3633 Size: 552 1390 111 515 111 36 261 144 Cuts at: 3633 4616 4948 513 Size: 983 332 3228 Fragments arranged by size: 3228 1390 983 552 515 332 261 144 111 111 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6924 Fragments arranged by size: 6924 683 56 BtsI GCAGTG_nn' Cuts at: 2849 3594 5179 5973 5993 2849 Size: 745 1585 794 20 4519 Fragments arranged by size: 4519 1585 794 745 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7663 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7628 DraI TTT'AAA Cuts at: 4967 4967 Size: 7663 DraIII CAC_nnn'GTG Cuts at: 3138 3138 Size: 7663 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2051 6670 809 Size: 507 735 4619 1802 Fragments arranged by size: 4619 1802 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2176 2820 Size: 54 1425 50 275 18 207 139 644 Cuts at: 2820 3636 3648 5110 5999 7628 8 Size: 816 12 1462 889 1629 43 Fragments arranged by size: 1629 1462 1425 889 816 644 275 207 139 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2037 2176 5110 1537 Size: 275 225 139 2934 4090 Fragments arranged by size: 4090 2934 275 225 139 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 2762 2907 3218 3330 3336 3945 4121 Size: 1298 111 145 311 112 6 609 176 Cuts at: 4121 5593 1353 Size: 1472 3423 Fragments arranged by size: 3423 1472 1298 609 311 176 145 112 111 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4277 6250 6624 6770 387 Size: 1064 138 2688 1973 374 146 1280 Fragments arranged by size: 2688 1973 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3180 4318 3180 Size: 1138 6525 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2432 2444 2450 2546 2582 3425 Size: 393 520 280 12 6 96 36 843 Cuts at: 3425 3863 4103 4109 4181 5726 6323 7110 7378 Size: 438 240 6 72 1545 597 787 268 Cuts at: 7378 1239 Size: 1524 Fragments arranged by size: 1545 1524 843 787 597 520 438 393 280 268 240 96 72 36 12 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 2411 3743 2411 Size: 1332 6331 EcoO109I rG'GnC_Cy Cuts at: 2220 2643 3319 3787 2220 Size: 423 676 468 6096 Fragments arranged by size: 6096 676 468 423 EcoRI G'AATT_C Cuts at: 3853 4972 5350 3853 Size: 1119 378 6166 Fragments arranged by size: 6166 1119 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2737 2737 Size: 7663 FspI TGC'GCA Cuts at: 6169 6169 Size: 7663 HaeII r_GCGC'y Cuts at: 1678 2380 3436 6810 1678 Size: 702 1056 3374 2531 Fragments arranged by size: 3374 2531 1056 702 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2321 2353 3114 Size: 32 135 32 683 32 34 32 761 Cuts at: 3114 3146 6304 6336 6378 6410 1373 Size: 32 3158 32 42 32 2626 Fragments arranged by size: 3158 2626 761 683 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2014 3456 4498 5007 5253 669 Size: 988 281 76 1442 1042 509 246 3079 Fragments arranged by size: 3079 1442 1042 988 509 281 246 76 HindIII A'AGCT_T Cuts at: 748 3805 4388 748 Size: 3057 583 4023 Fragments arranged by size: 4023 3057 583 HpaI GTT'AAC Cuts at: 5253 5253 Size: 7663 KpnI G_GTAC'C Cuts at: 4988 4988 Size: 7663 MfeI C'AATT_G Cuts at: 5262 5262 Size: 7663 MluI A'CGCG_T Cuts at: 7539 7539 Size: 7663 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3702 3883 4602 6777 6961 1305 Size: 2397 181 719 2175 184 2007 Fragments arranged by size: 2397 2175 2007 719 184 181 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 3650 10 Size: 54 1425 343 990 828 4023 Fragments arranged by size: 4023 1425 990 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2367 2556 3023 3107 3272 3578 Size: 648 1035 166 189 467 84 165 306 Cuts at: 3578 4004 4196 4712 5621 5980 6139 518 Size: 426 192 516 909 359 159 2042 Fragments arranged by size: 2042 1035 909 648 516 467 426 359 306 192 189 166 165 159 84 MspA1I CmG'CkG Cuts at: 1606 3315 4021 4138 4834 5079 5756 6904 7149 Size: 1709 706 117 696 245 677 1148 245 Cuts at: 7149 1606 Size: 2120 Fragments arranged by size: 2120 1709 1148 706 696 677 245 245 117 NaeI GCC'GGC Cuts at: 2180 3437 2180 Size: 1257 6406 NcoI C'CATG_G Cuts at: 513 1065 3081 3489 3633 513 Size: 552 2016 408 144 4543 Fragments arranged by size: 4543 2016 552 408 144 NdeI CA'TA_TG Cuts at: 387 387 Size: 7663 NgoMIV G'CCGG_C Cuts at: 2178 3435 2178 Size: 1257 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7663 NotI GC'GGCC_GC Cuts at: 5110 5110 Size: 7663 NsiI A_TGCA'T Cuts at: 3989 3989 Size: 7663 NspI r_CATG'y Cuts at: 1132 1229 4465 5021 6566 1132 Size: 97 3236 556 1545 2229 Fragments arranged by size: 3236 2229 1545 556 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7663 PciI A'CATG_T Cuts at: 4461 6562 4461 Size: 2101 5562 PflMI CCAn_nnn'nTGG Cuts at: 1108 4702 1108 Size: 3594 4069 PfoI T'CCnGG_A Cuts at: 2409 3738 4740 2409 Size: 1329 1002 5332 Fragments arranged by size: 5332 1329 1002 PmeI GTTT'AAAC Cuts at: 4967 4967 Size: 7663 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5732 5764 7245 7277 5732 Size: 32 1481 32 6118 Fragments arranged by size: 6118 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3319 3787 3319 Size: 468 7195 PshAI GACnn'nnGTC Cuts at: 1613 2240 1613 Size: 627 7036 PsiI TTA'TAA Cuts at: 5233 5233 Size: 7663 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2764 2796 2764 Size: 32 7631 PstI C_TGCA'G Cuts at: 830 3164 4592 5015 830 Size: 2334 1428 423 3478 Fragments arranged by size: 3478 2334 1428 423 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7663 PvuII CAG'CTG Cuts at: 4021 4834 4021 Size: 813 6850 SacI G_AGCT'C Cuts at: 3182 4320 3182 Size: 1138 6525 SalI G'TCGA_C Cuts at: 1936 5005 1936 Size: 3069 4594 SbfI CC_TGCA'GG Cuts at: 5015 5015 Size: 7663 ScaI AGT'ACT Cuts at: 2024 5911 7623 2024 Size: 3887 1712 2064 Fragments arranged by size: 3887 2064 1712 SexAI A'CCwGG_T Cuts at: 2958 4701 2958 Size: 1743 5920 SfcI C'TryA_G Cuts at: 826 1045 3160 3622 4588 5011 6146 6827 7018 Size: 219 2115 462 966 423 1135 681 191 Cuts at: 7018 826 Size: 1471 Fragments arranged by size: 2115 1471 1135 966 681 462 423 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7663 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7663 SmaI CCC'GGG Cuts at: 1872 2681 3912 4990 1872 Size: 809 1231 1078 4545 Fragments arranged by size: 4545 1231 1078 809 SmlI C'TyrA_G Cuts at: 820 1017 1943 5767 6668 6930 820 Size: 197 926 3824 901 262 1553 Fragments arranged by size: 3824 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5454 493 Size: 4961 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7663 SphI G_CATG'C Cuts at: 1132 5021 1132 Size: 3889 3774 SrfI GCCC'GGGC Cuts at: 3912 3912 Size: 7663 SspI AAT'ATT Cuts at: 5 52 5587 5 Size: 47 5535 2081 Fragments arranged by size: 5535 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7663 StyI C'CwwG_G Cuts at: 513 1065 3081 3489 3633 3645 4644 4752 5102 Size: 552 2016 408 144 12 999 108 350 Cuts at: 5102 513 Size: 3074 Fragments arranged by size: 3074 2016 999 552 408 350 144 108 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3035 5690 5707 5860 6045 6490 325 Size: 1255 1455 2655 17 153 185 445 1498 Fragments arranged by size: 2655 1498 1455 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 2163 2780 Size: 274 80 33 51 1443 45 141 617 Cuts at: 2780 2793 2994 3015 3924 4188 4464 5909 7621 Size: 13 201 21 909 264 276 1445 1712 Cuts at: 7621 96 Size: 138 Fragments arranged by size: 1712 1445 1443 909 617 276 274 264 201 141 138 80 51 45 33 21 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4493 4525 7623 7655 4493 Size: 32 3098 32 4501 Fragments arranged by size: 4501 3098 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2960 4733 1316 Size: 1644 1773 4246 Fragments arranged by size: 4246 1773 1644 XbaI T'CTAG_A Cuts at: 4999 4999 Size: 7663 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 3874 4558 2797 Size: 1077 684 5902 Fragments arranged by size: 5902 1077 684 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7663 XmaI C'CCGG_G Cuts at: 1870 2679 3910 4988 1870 Size: 809 1231 1078 4545 Fragments arranged by size: 4545 1231 1078 809 XmnI GAAnn'nnTTC Cuts at: 5792 5792 Size: 7663 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5471 276 Size: 53 83 186 936 3937 2468 Fragments arranged by size: 3937 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SwaI