Restriction map for
pFN21AE1116
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I MaeI MaeIII
MamI MfeI MflI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 494 TCCGGA T^CCGGA
AlwI 512 GGATC GGATC(4/5)
AspHI 262 GAGCAC G[AT]GC[AT]^C
BcgI 386 CGAAGCACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 406 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 280 CTGGAG CTGGAG(16/14)
BsaAI 592 TACGTG [CT]AC^GT[GA]
BsaWI 494 TCCGGA [AT]^CCGG[AT]
BseAI 494 TCCGGA T^CCGGA
BsiEI 39 CGACCG CG[GA][CT]^CG
BsiHKAI 262 GAGCAC G[AT]GC[AT]^C
BspEI 494 TCCGGA T^CCGGA
BstBI 15 TTCGAA TT^CGAA
BstF5I 335 CATCC GGATG(2/0)
BstNI 111 CCAGG CC^[AT]GG
DdeI 536 CTAAG C^T[ACGT]AG
DraII 83 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 99 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 531 CCTGACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 83 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 111 CCAGG ^CC[AT]GG
FokI 335 CATCC GGATG(9/13)
HaeII 572 AGCGCC [GA]GCGC^[CT]
HphI 482 TCACC GGTGA(8/7)
MaeII 593 ACGT A^CGT
MroI 494 TCCGGA T^CCGGA
MvaI 111 CCAGG CC^[AT]GG
NcoI 302 CCATGG C^CATGG
NspV 15 TTCGAA TT^CGAA
PpuMI 83 GGGACCT [GA]G^G[AT]CC[CT]
PstI 544 CTGCAG CTGCA^G
PvuII 118 CAGCTG CAG^CTG
SfaNI 334 GCATC GCATC(5/9)
SfcI 544 CTGCAG C^T[GA][CT]AG
SfuI 15 TTCGAA TT^CGAA
SmaI 201 CCCGGG CCC^GGG
SspI 379 AATATT AAT^ATT
XmaCI 201 CCCGGG C^CCGGG
XmaI 201 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 228 GTAC GT^AC
591 GTAC
AvaI 80 CTCGGG C^[CT]CG[GA]G
201 CCCGGG
BanII 210 GGGCTC G[GA]GC[CT]^C
562 GGGCTC
BbsI 33 GTCTTC GAAGAC(2/6)
357 GTCTTC
BcnI 201 CCCGG CC^[GC]GG
202 CCGGG
BpuAI 33 GTCTTC GAAGAC(2/6)
357 GTCTTC
BseRI 58 GAGGAG GAGGAG(10/8)
160 GAGGAG
BsoBI 80 CTCGGG C^[CT]CG[GA]G
201 CCCGGG
CfoI 268 GCGC GCG^C
573 GCGC
DpnII 342 GATC ^GATC
513 GATC
EcoT14I 45 CCAAGG C^C[AT][AT]GG
302 CCATGG
HhaI 268 GCGC GCG^C
573 GCGC
HinP1I 268 GCGC G^CGC
573 GCGC
KspI 186 CCGCGG CCGC^GG
291 CCGCGG
MboI 342 GATC ^GATC
513 GATC
MboII 34 TCTTC GAAGA(8/7)
358 TCTTC
NciI 201 CCCGG CC^[GC]GG
202 CCGGG
NdeII 342 GATC ^GATC
513 GATC
NlaIV 83 GGGACC GG[ACGT]^[ACGT]CC
521 GGGACC
NspI 125 ACATGC [GA]CATG^[CT]
392 ACATGC
RsaI 228 GTAC GT^AC
591 GTAC
SacII 186 CCGCGG CCGC^GG
291 CCGCGG
Sau3AI 342 GATC ^GATC
513 GATC
SmlI 28 CTCAAG C^T[CT][GA]AG
565 CTCAAG
StyI 45 CCAAGG C^C[AT][AT]GG
302 CCATGG
TspRI 330 CACTG CA[GC]TG[ACGT][ACGT]^
437 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstUI Cac8I
Cfr13I DsaI FauI Fnu4HI HaeIII
HapII HpaII ItaI MnlI MspA1I
MspI MvnI MwoI NlaIII Sau96I
ScrFI TaqI TseI TthHB8I