################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 1033 GTATAC GT^[AC][GT]AC Alw44I 83 GTGCAC G^TGCAC AocI 1710 CCTCAGG CC^T[ACGT]AGG ApaLI 83 GTGCAC G^TGCAC Asp700I 118 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 919 GGATAC GTATCC(6/5) BclI 1356 TGATCA T^GATCA BcnI 10 CCGGG CC^[GC]GG BseRI 353 CTCCTC GAGGAG(10/8) Bsp1407I 1282 TGTACA T^GTACA BsrGI 1282 TGTACA T^GTACA Bst1107I 1033 GTATAC GTA^TAC BstXI 658 CCACTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1033 GTATAC GTA^TAC Bsu36I 1710 CCTCAGG CC^T[ACGT]AGG Eco81I 1710 CCTCAGG CC^T[ACGT]AGG EcoRV 730 GATATC GAT^ATC FbaI 1356 TGATCA T^GATCA HapII 10 CCGG C^CGG HincII 1599 GTCAAC GT[CT]^[GA]AC HindII 1599 GTCAAC GT[CT]^[GA]AC HindIII 703 AAGCTT A^AGCTT HpaII 10 CCGG C^CGG MaeII 1702 ACGT A^CGT MspI 10 CCGG C^CGG NciI 10 CCGGG CC^[GC]GG NheI 1575 GCTAGC G^CTAGC NspI 482 ACATGC [GA]CATG^[CT] PflMI 58 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 139 GACTC GAGTC(4/5) SacI 871 GAGCTC GAGCT^C SspBI 1282 TGTACA T^GTACA SwaI 341 ATTTAAAT ATTT^AAAT Van91I 58 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 118 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 179 GAATTT [GA]^AATT[CT] 345 AAATTC AfaI 1283 GTAC GT^AC 1818 GTAC AlwNI 1250 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 1744 CAGAACCTG ApoI 179 GAATTT [GA]^AATT[CT] 345 AAATTC AspHI 83 GTGCAC G[AT]GC[AT]^C 871 GAGCTC AspI 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACGTTGTC BbsI 1716 GAAGAC GAAGAC(2/6) 965 GTCTTC BpmI 56 CTCCAG CTGGAG(16/14) 406 CTCCAG BpuAI 1716 GAAGAC GAAGAC(2/6) 965 GTCTTC BsgI 27 GTGCAG GTGCAG(16/14) 416 GTGCAG BsiHKAI 83 GTGCAC G[AT]GC[AT]^C 871 GAGCTC BsmFI 513 GGGAC GGGAC(10/14) 1707 GTCCC BstYI 285 GGATCT [GA]^GATC[CT] 1462 GGATCT MflI 285 GGATCT [GA]^GATC[CT] 1462 GGATCT MslI 37 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1321 CACTGCCATG NdeI 799 CATATG CA^TATG 1690 CATATG PflFI 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACGTTGTC PstI 859 CTGCAG CTGCA^G 1672 CTGCAG RsaI 1283 GTAC GT^AC 1818 GTAC SapI 121 GCTCTTC GCTCTTC(1/4) 873 GCTCTTC SfcI 859 CTGCAG C^T[GA][CT]AG 1672 CTGCAG TaqI 69 TCGA T^CGA 1350 TCGA Tsp45I 152 GTGAC ^GT[GC]AC 1314 GTCAC Tth111I 154 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1701 GACGTTGTC TthHB8I 69 TCGA T^CGA 1350 TCGA XhoII 285 GGATCT [GA]^GATC[CT] 1462 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AluI AlwI BanII BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI