(Circular) (Six-base) MAPSORT of: pFN21AE6485.seq Check: 6567 from: 1 to: 6571 pFN21AE6485 6571 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3039 3914 1937 Size: 1102 875 4594 Fragments arranged by size: 4594 1102 875 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3240 3259 4634 6018 6286 1632 Size: 1608 19 1375 1384 268 1917 Fragments arranged by size: 1917 1608 1384 1375 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 3260 3754 4314 5886 3260 Size: 494 560 1572 3945 Fragments arranged by size: 3945 1572 560 494 ApaLI G'TGCA_C Cuts at: 2088 4628 5784 2088 Size: 2540 1156 2875 Fragments arranged by size: 2875 2540 1156 ApoI r'AATT_y Cuts at: 1493 2184 2350 3880 4106 4258 6367 1493 Size: 691 166 1530 226 152 2109 1697 Fragments arranged by size: 2109 1697 1530 691 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 3896 1870 Size: 73 1953 4545 Fragments arranged by size: 4545 1953 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3892 5347 618 Size: 325 217 627 2105 1455 1842 Fragments arranged by size: 2105 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2880 3127 3890 1391 Size: 636 853 247 763 4072 Fragments arranged by size: 4072 853 763 636 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2963 3728 928 Size: 971 1064 765 3771 Fragments arranged by size: 3771 1064 971 765 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4762 4796 1903 Size: 34 2825 34 3678 Fragments arranged by size: 3678 2825 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2918 4463 5673 2918 Size: 1545 1210 3816 Fragments arranged by size: 3816 1545 1210 BclI T'GATC_A Cuts at: 1548 3361 1548 Size: 1813 4758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5182 136 Size: 107 122 71 631 605 3510 1525 Fragments arranged by size: 3510 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 2092 4632 5788 1790 Size: 302 2540 1156 2573 Fragments arranged by size: 2573 2540 1156 302 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2377 2680 4302 Size: 416 53 83 399 24 960 303 1622 Cuts at: 4302 5260 442 Size: 958 1753 Fragments arranged by size: 1753 1622 960 958 416 399 303 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3584 1056 Size: 2528 4043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2046 2396 5231 1239 Size: 807 350 2835 2579 Fragments arranged by size: 2835 2579 807 350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5234 882 Size: 617 3735 2219 Fragments arranged by size: 3735 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4362 493 Size: 711 3158 2702 Fragments arranged by size: 3158 2702 711 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4378 4760 5395 275 Size: 53 83 186 936 2845 382 635 1451 Fragments arranged by size: 2845 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5004 5402 5676 5823 6234 5004 Size: 398 274 147 411 5341 Fragments arranged by size: 5341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6346 Fragments arranged by size: 6346 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2349 1186 Size: 1163 5408 BseYI C'CCAG_C Cuts at: 2020 3841 5774 6270 2020 Size: 1821 1933 496 2321 Fragments arranged by size: 2321 1933 1821 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2442 1163 Size: 573 317 389 5292 Fragments arranged by size: 5292 573 389 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2092 2880 4632 4717 5788 2092 Size: 788 1752 85 1071 2875 Fragments arranged by size: 2875 1752 1071 788 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2092 2880 3127 3890 4632 4717 Size: 399 237 65 788 247 763 742 85 Cuts at: 4717 5788 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 788 763 742 399 247 237 85 65 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 4460 6190 4460 Size: 1730 4841 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3926 844 Size: 3082 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5215 5402 1700 Size: 110 3405 187 2869 Fragments arranged by size: 3405 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3287 96 Size: 3191 3380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstXI CCAn_nnnn'nTGG Cuts at: 2670 2670 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2290 3467 3901 4268 4653 4670 6122 6208 Size: 318 1177 434 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1177 434 385 367 346 318 86 17 12 BstZ17I GTA'TAC Cuts at: 3040 3040 Size: 6571 Bsu36I CC'TnA_GG Cuts at: 3716 3716 Size: 6571 BtgI C'CryG_G Cuts at: 513 1065 2004 2047 3441 3828 513 Size: 552 939 43 1394 387 3256 Fragments arranged by size: 3256 1394 939 552 387 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 2036 3325 3367 4087 4881 4901 2036 Size: 1289 42 720 794 20 3706 Fragments arranged by size: 3706 1289 794 720 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2419 2454 529 Size: 35 1855 35 4646 Fragments arranged by size: 4646 1855 35 35 DraI TTT'AAA Cuts at: 2349 2613 2961 3875 2349 Size: 264 348 914 5045 Fragments arranged by size: 5045 914 348 264 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4018 4907 6536 Size: 54 1425 50 275 18 2188 889 1629 Cuts at: 6536 8 Size: 43 Fragments arranged by size: 2188 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2367 2885 4501 1353 Size: 780 234 518 1616 3423 Fragments arranged by size: 3423 1616 780 518 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2878 2878 Size: 6571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2396 3240 3259 4634 5231 6018 Size: 393 414 350 844 19 1375 597 787 Cuts at: 6018 6286 1239 Size: 268 1524 Fragments arranged by size: 1524 1375 844 787 597 414 393 350 268 19 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 EcoRV GAT'ATC Cuts at: 2737 2737 Size: 6571 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2987 2987 Size: 6571 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2211 2243 3459 Size: 32 135 32 495 32 112 32 1216 Cuts at: 3459 3491 3800 3832 5212 5244 5286 5318 1373 Size: 32 309 32 1380 32 42 32 2626 Fragments arranged by size: 2626 1380 1216 495 309 135 112 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3915 4161 669 Size: 988 281 1668 309 246 3079 Fragments arranged by size: 3079 1668 988 309 281 246 HindIII A'AGCT_T Cuts at: 748 2708 748 Size: 1960 4611 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2401 5685 5869 1305 Size: 1096 3284 184 2007 Fragments arranged by size: 3284 2007 1096 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4749 Fragments arranged by size: 4749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 3330 4529 4888 5047 518 Size: 648 880 1284 1199 359 159 2042 Fragments arranged by size: 2042 1284 1199 880 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3987 4664 5812 6057 1606 Size: 2381 677 1148 245 2120 Fragments arranged by size: 2381 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2047 3441 3828 513 Size: 552 939 43 1394 387 3256 Fragments arranged by size: 3256 1394 939 552 387 43 NdeI CA'TA_TG Cuts at: 387 2805 3696 387 Size: 2418 891 3262 Fragments arranged by size: 3262 2418 891 NheI G'CTAG_C Cuts at: 1052 3580 1052 Size: 2528 4043 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 2491 3929 5474 1132 Size: 97 1262 1438 1545 2229 Fragments arranged by size: 2229 1545 1438 1262 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 1108 Size: 961 5610 PfoI T'CCnGG_A Cuts at: 2062 2062 Size: 6571 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 3302 4141 3302 Size: 839 5732 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3023 3055 3271 3303 3023 Size: 32 216 32 6291 Fragments arranged by size: 6291 216 32 32 PstI C_TGCA'G Cuts at: 830 2868 3681 3923 830 Size: 2038 813 242 3478 Fragments arranged by size: 3478 2038 813 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 SacI G_AGCT'C Cuts at: 2880 2880 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SapI GCTCTTCn'nnn_ Cuts at: 2133 2885 2133 Size: 752 5819 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SfcI C'TryA_G Cuts at: 826 1045 2864 3677 3919 5054 5735 5926 826 Size: 219 1819 813 242 1135 681 191 1471 Fragments arranged by size: 1819 1471 1135 813 681 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4675 5576 5838 820 Size: 197 926 2732 901 262 1553 Fragments arranged by size: 2732 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6571 StyI C'CwwG_G Cuts at: 513 1065 2004 2047 3008 3441 3557 3828 4010 Size: 552 939 43 961 433 116 271 182 Cuts at: 4010 513 Size: 3074 Fragments arranged by size: 3074 961 939 552 433 271 182 116 43 SwaI ATTT'AAAT Cuts at: 2349 2349 Size: 6571 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 3855 4598 4615 4768 4953 5398 Size: 1255 747 1528 743 17 153 185 445 Cuts at: 5398 325 Size: 1498 Fragments arranged by size: 1528 1498 1255 747 743 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3287 3822 4817 Size: 274 80 33 51 1443 1310 535 995 Cuts at: 4817 6529 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1310 995 535 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2511 2543 6531 6563 2511 Size: 32 3988 32 2519 Fragments arranged by size: 3988 2519 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2162 3709 1316 Size: 846 1547 4178 Fragments arranged by size: 4178 1547 846 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6571 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 2127 4700 2127 Size: 2573 3998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI