################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AccI AccIII AclI AcsI AflII AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BcgI BciVI BfaI BfrI BlnI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 563 GGTACC G^GTACC AflIII 904 ACGTGT A^C[GA][CT]GT AgeI 1973 ACCGGT A^CCGGT Alw44I 1948 GTGCAC G^TGCAC AlwNI 127 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1948 GTGCAC G^TGCAC Asp718I 563 GGTACC G^GTACC AspI 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 2262 CCTCAGC CCTCAGC(-5/-2) BglII 1373 AGATCT A^GATCT BlpI 303 GCTCAGC GC^T[ACGT]AGC Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC BsaI 287 GAGACC GGTCTC(1/5) Bsp1407I 311 TGTACA T^GTACA BsrBI 142 CCGCTC CCGCTC(-3/-3) BsrGI 311 TGTACA T^GTACA BssSI 1003 CACGAG CACGAG(-5/-1) BstEII 2286 GGTCACC G^GT[ACGT]ACC BstPI 2286 GGTCACC G^GT[ACGT]ACC BstXI 2066 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 303 GCTCAGC GC^T[ACGT]AGC DraIII 1003 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 978 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2286 GGTCACC G^GT[ACGT]ACC HindIII 1757 AAGCTT A^AGCTT HinfI 1751 GAGTC G^A[ACGT]TC KpnI 563 GGTACC GGTAC^C NaeI 989 GCCGGC GCC^GGC NcoI 386 CCATGG C^CATGG NgoMIV 989 GCCGGC G^CCGGC NspI 935 GCATGT [GA]CATG^[CT] PaeR7I 544 CTCGAG C^TCGAG PflFI 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1500 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1973 ACCGGT A^CCGGT PleI 1751 GAGTC GAGTC(4/5) SacI 462 GAGCTC GAGCT^C SapI 7 GAAGAGC GCTCTTC(1/4) ScaI 1325 AGTACT AGT^ACT SspBI 311 TGTACA T^GTACA Tth111I 2242 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1500 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 544 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AatII 594 GACGTC GACGT^C 679 GACGTC AlwI 1134 GATCC GGATC(4/5) 160 GGATC ApaI 796 GGGCCC GGGCC^C 2217 GGGCCC BalI 614 TGGCCA TGG^CCA 2009 TGGCCA BclI 1340 TGATCA T^GATCA 1388 TGATCA BsaWI 1312 ACCGGA [AT]^CCGG[AT] 1973 ACCGGT BsiEI 633 CGACCG CG[GA][CT]^CG 1908 CGACCG BstAPI 126 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 935 GCATGTACTGC BstYI 1133 AGATCC [GA]^GATC[CT] 1373 AGATCT EarI 1767 CTCTTC CTCTTC(1/4) 7 GAAGAG FbaI 1340 TGATCA T^GATCA 1388 TGATCA Ksp632I 1767 CTCTTC CTCTTC(1/4) 7 GAAGAG MflI 1133 AGATCC [GA]^GATC[CT] 1373 AGATCT MluNI 614 TGGCCA TGG^CCA 2009 TGGCCA MscI 614 TGGCCA TGG^CCA 2009 TGGCCA MseI 1063 TTAA T^TAA 1201 TTAA PpuMI 337 GGGTCCT [GA]G^G[AT]CC[CT] 726 GGGACCC StuI 1058 AGGCCT AGG^CCT 2057 AGGCCT Tru9I 1063 TTAA T^TAA 1201 TTAA XhoII 1133 AGATCC [GA]^GATC[CT] 1373 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI AvaII BanI BanII BbeI BbrPI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PmaCI PmlI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XmaCI XmaI