################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AluI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 34 GTCGAC GT^[AC][GT]AC AcsI 244 AAATTT [GA]^AATT[CT] AfaI 438 GTAC GT^AC AflIII 402 ACGTGT A^C[GA][CT]GT AlwNI 278 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 244 AAATTT [GA]^AATT[CT] Asp700I 100 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 310 GGACC G^G[AT]CC BbvI 346 GCTGC GCAGC(8/12) BglI 206 GCCATAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 122 CTGGAG CTGGAG(16/14) BsiYI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 230 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 471 CATTGC GCAATG(2/0) BssKI 172 CCAGG ^CC[ACGT]GG BstNI 172 CCAGG CC^[AT]GG DpnII 465 GATC ^GATC DrdI 25 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 172 CCAGG ^CC[AT]GG HinfI 84 GATTC G^A[ACGT]TC MaeII 402 ACGT A^CGT MboI 465 GATC ^GATC MboII 462 GAAGA GAAGA(8/7) MnlI 261 CCTC CCTC(7/6) MspA1I 95 CAGCGG C[AC]G^C[GT]G MvaI 172 CCAGG CC^[AT]GG NdeII 465 GATC ^GATC NspI 375 GCATGT [GA]CATG^[CT] RsaI 438 GTAC GT^AC SalI 34 GTCGAC G^TCGAC Sau3AI 465 GATC ^GATC ScrFI 172 CCAGG CC^[ACGT]GG SspI 409 AATATT AAT^ATT TaqI 35 TCGA T^CGA TfiI 84 GATTC G^A[AT]TC TseI 346 GCTGC G^C[AT]GC TthHB8I 35 TCGA T^CGA XcmI 207 CCATAATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 100 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 54 CCGC CCGC(-3/-1) 97 GCGG BstF5I 264 CATCC GGATG(2/0) 366 GGATG Cac8I 41 GCAAGC GC[ACGT]^[ACGT]GC 371 GCAAGC Cfr13I 51 GGGCC G^G[ACGT]CC 310 GGACC DdeI 115 CTGAG C^T[ACGT]AG 356 CTGAG DsaI 296 CCATGG C^C[GA][CT]GG 422 CCATGG Fnu4HI 53 GCCGC GC^[ACGT]GC 346 GCTGC FokI 264 CATCC GGATG(9/13) 366 GGATG HaeIII 52 GGCC GG^CC 381 GGCC HincII 4 GTCAAC GT[CT]^[GA]AC 34 GTCGAC HindII 4 GTCAAC GT[CT]^[GA]AC 34 GTCGAC ItaI 53 GCCGC GC^[ACGT]GC 346 GCTGC MaeIII 194 GTGAC ^GT[ACGT]AC 221 GTGAC MwoI 206 GCCATAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 449 GCAAGACCAGC NcoI 296 CCATGG C^CATGG 422 CCATGG Sau96I 51 GGGCC G^G[ACGT]CC 310 GGACC SfaNI 56 GCATC GCATC(5/9) 287 GCATC Tsp45I 194 GTGAC ^GT[GC]AC 221 GTGAC TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^ 216 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AspHI BmyI BsaJI BsiHKAI Bsp1286I BsrI EcoT14I HphI NlaIII NlaIV StyI Tsp509I