################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT14I FauI FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 45 CGCG CG^CG AciI 44 CCGC CCGC(-3/-1) AflIII 1280 ACACGT A^C[GA][CT]GT AvaII 2010 GGACC G^G[AT]CC BalI 1407 TGGCCA TGG^CCA BanII 1140 GAGCCC G[GA]GC[CT]^C BciVI 1380 GGATAC GTATCC(6/5) BcnI 62 CCGGG CC^[GC]GG BglI 43 GCCGCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1949 CTCCAG CTGGAG(16/14) BsaI 256 GAGACC GGTCTC(1/5) BsaJI 1695 CCCTGG C^C[ACGT][ACGT]GG BsgI 17 CTGCAC GTGCAG(16/14) BsmFI 232 GGGAC GGGAC(10/14) BstAPI 2218 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 6 GGTTACC G^GT[ACGT]ACC BstPI 6 GGTTACC G^GT[ACGT]ACC BstUI 45 CGCG CG^CG Cfr13I 2010 GGACC G^G[ACGT]CC EaeI 1407 TGGCCA [CT]^GGCC[GA] EcoO65I 6 GGTTACC G^GT[ACGT]ACC EcoT22I 1457 ATGCAT ATGCA^T HapII 62 CCGG C^CGG HgaI 46 GCGTC GACGC(5/10) HpaII 62 CCGG C^CGG HphI 1174 GGTGA GGTGA(8/7) MaeII 1282 ACGT A^CGT MluNI 1407 TGGCCA TGG^CCA MscI 1407 TGGCCA TGG^CCA MspI 62 CCGG C^CGG MvnI 45 CGCG CG^CG NciI 62 CCGGG CC^[GC]GG NsiI 1457 ATGCAT ATGCA^T NspI 343 GCATGC [GA]CATG^[CT] PshAI 1693 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1810 CTGCAG CTGCA^G SapI 201 GAAGAGC GCTCTTC(1/4) Sau96I 2010 GGACC G^G[ACGT]CC SpeI 1326 ACTAGT A^CTAGT SphI 343 GCATGC GCATG^C XbaI 451 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Alw44I 838 GTGCAC G^TGCAC 1048 GTGCAC AlwI 517 GATCC GGATC(4/5) 118 GGATC ApaLI 838 GTGCAC G^TGCAC 1048 GTGCAC AseI 937 ATTAAT AT^TAAT 1732 ATTAAT AsnI 937 ATTAAT AT^TAAT 1732 ATTAAT BsiYI 305 CCATTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 359 CCAGTGTTAGG BslI 305 CCATTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 359 CCAGTGTTAGG CfoI 97 GCGC GCG^C 1288 GCGC HhaI 97 GCGC GCG^C 1288 GCGC HinP1I 97 GCGC G^CGC 1288 GCGC HincII 1674 GTTGAC GT[CT]^[GA]AC 2080 GTTGAC HindII 1674 GTTGAC GT[CT]^[GA]AC 2080 GTTGAC MspA1I 955 CAGCTG C[AC]G^C[GT]G 2090 CAGCTG PshBI 937 ATTAAT AT^TAAT 1732 ATTAAT PvuII 955 CAGCTG CAG^CTG 2090 CAGCTG Tsp45I 1157 GTGAC ^GT[GC]AC 1451 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI BbsI BbvI BfaI BglII BmyI BpuAI BsiHKAI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EarI EcoRI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScaI ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII