(Circular) (Six-base) MAPSORT of: pFN21AB5753.seq Check: 2162 from: 1 to: 6973 pFN21AB5753 6973 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 1937 Size: 2379 4594 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 2448 2966 2984 3695 3875 4016 5036 Size: 801 15 518 18 711 180 141 1020 Cuts at: 5036 6420 6688 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1020 801 711 518 268 180 141 18 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 3285 5872 6849 1203 Size: 2082 2587 977 1327 Fragments arranged by size: 2587 2082 1327 977 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6973 AlwNI CAG_nnn'CTG Cuts at: 4716 6288 4716 Size: 1572 5401 ApaLI G'TGCA_C Cuts at: 2843 3053 5030 6186 2843 Size: 210 1977 1156 3630 Fragments arranged by size: 3630 1977 1156 210 ApoI r'AATT_y Cuts at: 1493 2707 2779 3508 4116 4172 4262 4282 4508 Size: 1214 72 729 608 56 90 20 226 Cuts at: 4508 4660 6769 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1214 729 608 226 152 90 72 56 20 AseI AT'TA_AT Cuts at: 160 1032 2943 3738 5527 160 Size: 872 1911 795 1789 1606 Fragments arranged by size: 1911 1789 1606 872 795 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 4298 1870 Size: 73 2355 4545 Fragments arranged by size: 4545 2355 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2688 2721 2688 Size: 33 6940 BamHI G'GATC_C Cuts at: 4303 4670 4303 Size: 367 6606 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4294 5749 618 Size: 325 217 627 2507 1455 1842 Fragments arranged by size: 2507 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3149 4292 1391 Size: 1758 1143 4072 Fragments arranged by size: 4072 1758 1143 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3240 3534 3648 928 Size: 971 1341 294 114 4253 Fragments arranged by size: 4253 1341 971 294 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5164 5198 1903 Size: 34 3227 34 3678 Fragments arranged by size: 3678 3227 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3379 4865 6075 3379 Size: 1486 1210 4277 Fragments arranged by size: 4277 1486 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6973 BfrBI ATG'CAT Cuts at: 3464 3464 Size: 6973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 5584 136 Size: 107 122 71 631 605 382 3530 1525 Fragments arranged by size: 3530 1525 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 2018 2337 2753 6968 2018 Size: 319 416 4215 2023 Fragments arranged by size: 4215 2023 416 319 BlpI GC'TnA_GC Cuts at: 4390 4390 Size: 6973 Bme1580I G_kGCm'C Cuts at: 1790 2847 3057 5034 6190 1790 Size: 1057 210 1977 1156 2573 Fragments arranged by size: 2573 1977 1156 1057 210 BmgBI CAC'GTC Cuts at: 1318 3288 1318 Size: 1970 5003 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2169 2351 4704 Size: 416 53 83 399 24 752 182 2353 Cuts at: 4704 5662 442 Size: 958 1753 Fragments arranged by size: 2353 1753 958 752 416 399 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3939 5633 1239 Size: 2700 1694 2579 Fragments arranged by size: 2700 2579 1694 Bpu10I CC'TnA_GC Cuts at: 2031 2031 Size: 6973 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5098 5963 6261 5098 Size: 865 298 5810 Fragments arranged by size: 5810 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 5636 882 Size: 617 756 3381 2219 Fragments arranged by size: 3381 2219 756 617 BsaAI yAC'GTr Cuts at: 493 1204 4764 493 Size: 711 3560 2702 Fragments arranged by size: 3560 2702 711 BsaBI GATnn'nnATC Cuts at: 6967 6967 Size: 6973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4780 5162 5797 275 Size: 53 83 186 936 3247 382 635 1451 Fragments arranged by size: 3247 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5406 5804 6078 6225 6636 5406 Size: 398 274 147 411 5743 Fragments arranged by size: 5743 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6748 Fragments arranged by size: 6748 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6973 BseYI C'CCAG_C Cuts at: 6176 6672 6176 Size: 496 6477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2007 1163 Size: 573 271 6129 Fragments arranged by size: 6129 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 2847 3057 5034 5119 6190 2301 Size: 546 210 1977 85 1071 3084 Fragments arranged by size: 3084 1977 1071 546 210 85 BsmI GAATG_Cn' Cuts at: 4484 4577 4484 Size: 93 6880 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2847 3057 3149 4292 5034 5119 Size: 399 511 546 210 92 1143 742 85 Cuts at: 5119 6190 1391 Size: 1071 2174 Fragments arranged by size: 2174 1143 1071 742 546 511 399 210 92 85 BspEI T'CCGG_A Cuts at: 6636 6636 Size: 6973 BspHI T'CATG_A Cuts at: 4862 6592 4862 Size: 1730 5243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4328 844 Size: 3484 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4860 1394 Size: 429 3037 3507 Fragments arranged by size: 3507 3037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4276 5468 5642 65 Size: 1183 3028 1192 174 1396 Fragments arranged by size: 3028 1396 1192 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5617 5804 1700 Size: 110 3807 187 2869 Fragments arranged by size: 3807 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 5033 6045 5033 Size: 1012 5961 BstAPI GCAn_nnn'nTGC Cuts at: 794 4229 794 Size: 3435 3538 BstBI TT'CG_AA Cuts at: 5777 5777 Size: 6973 BstEII G'GTnAC_C Cuts at: 1184 2011 1184 Size: 827 6146 BstYI r'GATC_y Cuts at: 1972 2018 2123 2337 2521 2753 4303 4670 5055 Size: 46 105 214 184 232 1550 367 385 Cuts at: 5055 5072 6524 6610 6622 6968 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1550 1452 385 367 346 232 214 184 105 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5482 Fragments arranged by size: 5482 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6234 Fragments arranged by size: 6234 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4489 5283 5303 3349 Size: 1140 794 20 5019 Fragments arranged by size: 5019 1140 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6938 DraI TTT'AAA Cuts at: 4277 4277 Size: 6973 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3412 4420 5309 Size: 54 1425 50 275 18 1582 1008 889 Cuts at: 5309 6938 8 Size: 1629 43 Fragments arranged by size: 1629 1582 1425 1008 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 3201 3375 3516 3561 3567 4903 1353 Size: 848 1000 174 141 45 6 1336 3423 Fragments arranged by size: 3423 1336 1000 848 174 141 45 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5560 5934 6080 387 Size: 1064 138 3971 374 146 1280 Fragments arranged by size: 3971 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2448 2966 2984 3695 3875 3939 Size: 393 801 15 518 18 711 180 64 Cuts at: 3939 4016 5036 5633 6420 6688 1239 Size: 77 1020 597 787 268 1524 Fragments arranged by size: 1524 1020 801 787 711 597 518 393 268 180 77 64 18 15 EcoRI G'AATT_C Cuts at: 2707 4116 4172 4282 4660 2707 Size: 1409 56 110 378 5020 Fragments arranged by size: 5020 1409 378 110 56 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 6120 1678 Size: 4442 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2795 2827 5614 Size: 32 135 32 689 32 502 32 2787 Cuts at: 5614 5646 5688 5720 1373 Size: 32 42 32 2626 Fragments arranged by size: 2787 2626 689 502 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3681 4087 4317 4563 669 Size: 988 281 1743 406 230 246 3079 Fragments arranged by size: 3079 1743 988 406 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 6973 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 4572 4572 Size: 6973 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2417 6087 6271 1305 Size: 1112 3670 184 2007 Fragments arranged by size: 3670 2007 1112 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3414 10 Size: 54 1425 343 1582 3569 Fragments arranged by size: 3569 1582 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4931 5290 5449 518 Size: 648 3765 359 159 2042 Fragments arranged by size: 3765 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2962 4097 4389 5066 6214 6459 1606 Size: 1356 1135 292 677 1148 245 2120 Fragments arranged by size: 2120 1356 1148 1135 677 292 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5482 Fragments arranged by size: 5482 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NsiI A_TGCA'T Cuts at: 3466 3466 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 2352 4331 5876 1132 Size: 97 1123 1979 1545 2229 Fragments arranged by size: 2229 1979 1545 1123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 5872 5872 Size: 6973 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6973 PfoI T'CCnGG_A Cuts at: 3487 3487 Size: 6973 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3702 1613 Size: 2089 4884 PsiI TTA'TAA Cuts at: 3221 4543 3221 Size: 1322 5651 PstI C_TGCA'G Cuts at: 830 3819 4325 830 Size: 2989 506 3478 Fragments arranged by size: 3478 2989 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 2962 4097 2962 Size: 1135 5838 SalI G'TCGA_C Cuts at: 1936 4315 1936 Size: 2379 4594 SapI GCTCTTCn'nnn_ Cuts at: 2201 2201 Size: 6973 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 2119 4064 4199 5221 6933 2119 Size: 1945 135 1022 1712 2159 Fragments arranged by size: 2159 1945 1712 1022 135 SfcI C'TryA_G Cuts at: 826 1045 2217 2484 3758 3815 4321 5456 6137 Size: 219 1172 267 1274 57 506 1135 681 Cuts at: 6137 6328 826 Size: 191 1471 Fragments arranged by size: 1471 1274 1172 1135 681 506 267 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 4300 1872 Size: 2428 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5077 5978 6240 820 Size: 197 926 3134 901 262 1553 Fragments arranged by size: 3134 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 3331 152 Size: 3179 3794 SphI G_CATG'C Cuts at: 1132 2352 4331 1132 Size: 1220 1979 3774 Fragments arranged by size: 3774 1979 1220 SspI AAT'ATT Cuts at: 5 52 4897 5 Size: 47 4845 2081 Fragments arranged by size: 4845 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6973 StyI C'CwwG_G Cuts at: 513 1065 2004 4412 513 Size: 552 939 2408 3074 Fragments arranged by size: 3074 2408 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 5000 5017 5170 5355 5800 325 Size: 1255 2133 1287 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1287 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2117 4062 4197 Size: 274 80 33 51 1443 140 1945 135 Cuts at: 4197 5219 6931 96 Size: 1022 1712 138 Fragments arranged by size: 1945 1712 1443 1022 274 140 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6933 6965 6933 Size: 32 6941 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6973 XbaI T'CTAG_A Cuts at: 2456 4309 2456 Size: 1853 5120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6973 XmaI C'CCGG_G Cuts at: 1870 4298 1870 Size: 2428 4545 XmnI GAAnn'nnTTC Cuts at: 5102 5102 Size: 6973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI