################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AclI AcsI AflII AhdI AlwI AocI ApoI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI HindIII HpaI KpnI MamI MfeI MflI MluNI MscI MseI MunI NdeI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 681 GACGTC GACGT^C AccI 1159 GTCGAC GT^[AC][GT]AC AccIII 1614 TCCGGA T^CCGGA AgeI 126 ACCGGT A^CCGGT Aor51HI 796 AGCGCT AGC^GCT AscI 1372 GGCGCGCC GG^CGCGCC AspI 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 344 TGCGCA TGC^GCA BbsI 466 GTCTTC GAAGAC(2/6) BbvCI 1386 CCTCAGC CCTCAGC(-5/-2) BcgI 296 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1293 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1293 GCTGAGC GC^T[ACGT]AGC BpuAI 466 GTCTTC GAAGAC(2/6) BseAI 1614 TCCGGA T^CCGGA BsgI 1030 GTGCAG GTGCAG(16/14) BspEI 1614 TCCGGA T^CCGGA BsrI 1334 CCAGT ACTGG(1/-1) BstAPI 1126 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 441 CATCC GGATG(2/0) BstXI 1056 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1293 GCTGAGC GC^T[ACGT]AGC DpnII 1519 GATC ^GATC EarI 883 GAAGAG CTCTTC(1/4) Eco47III 796 AGCGCT AGC^GCT EcoRV 952 GATATC GAT^ATC FokI 441 CATCC GGATG(9/13) FseI 1077 GGCCGGCC GGCCGG^CC FspI 344 TGCGCA TGC^GCA Ksp632I 883 GAAGAG CTCTTC(1/4) MboI 1519 GATC ^GATC MroI 1614 TCCGGA T^CCGGA NdeII 1519 GATC ^GATC NheI 841 GCTAGC G^CTAGC NotI 109 GCGGCCGC GC^GGCCGC PflFI 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 225 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 126 ACCGGT A^CCGGT PpuMI 498 GGGACCT [GA]G^G[AT]CC[CT] SalI 1159 GTCGAC G^TCGAC Sau3AI 1519 GATC ^GATC SmlI 244 CTCAAG C^T[CT][GA]AG TfiI 859 GAATC G^A[AT]TC Tth111I 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 225 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1460 CCACTGCCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 189 GTAC GT^AC 1590 GTAC AflIII 963 ACGCGT A^C[GA][CT]GT 1142 ACGCGT Alw44I 294 GTGCAC G^TGCAC 1558 GTGCAC ApaLI 294 GTGCAC G^TGCAC 1558 GTGCAC AspHI 294 GTGCAC G[AT]GC[AT]^C 1558 GTGCAC BfaI 754 CTAG C^TAG 842 CTAG BpmI 503 CTGGAG CTGGAG(16/14) 994 CTGGAG BseRI 364 GAGGAG GAGGAG(10/8) 820 GAGGAG BsiHKAI 294 GTGCAC G[AT]GC[AT]^C 1558 GTGCAC HincII 1159 GTCGAC GT[CT]^[GA]AC 1603 GTCAAC HindII 1159 GTCGAC GT[CT]^[GA]AC 1603 GTCAAC MaeI 754 CTAG C^TAG 842 CTAG MaeII 682 ACGT A^CGT 1025 ACGT MaeIII 1337 GTCAC ^GT[ACGT]AC 1529 GTGAC MboII 883 GAAGA GAAGA(8/7) 467 TCTTC MluI 963 ACGCGT A^CGCGT 1142 ACGCGT MslI 1057 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1339 CACTTCGGTG PleI 667 GACTC GAGTC(4/5) 733 GAGTC RsaI 189 GTAC GT^AC 1590 GTAC SfaNI 118 GCATC GCATC(5/9) 437 GCATC TaqI 1040 TCGA T^CGA 1160 TCGA Tsp45I 1337 GTCAC ^GT[GC]AC 1529 GTGAC TthHB8I 1040 TCGA T^CGA 1160 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsaWI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NlaIII NlaIV PstI PvuII SacII Sau96I ScrFI SfcI SfoI SgrAI SmaI StyI TseI TspRI XmaCI XmaI