(Circular) (Six-base) MAPSORT of: pFN21AE3425.seq Check: 1091 from: 1 to: 6331 pFN21AE3425 6331 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2771 3040 2329 Size: 442 269 5620 Fragments arranged by size: 5620 442 269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2690 4141 278 Size: 53 83 186 936 1154 1451 2468 Fragments arranged by size: 2468 1451 1154 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3165 3674 1937 Size: 1228 509 4594 Fragments arranged by size: 4594 1228 509 Acc65I G'GTAC_C Cuts at: 3652 3652 Size: 6331 AclI AA'CG_TT Cuts at: 1563 4458 4831 1563 Size: 2895 373 3063 Fragments arranged by size: 3063 2895 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4394 5778 6046 1632 Size: 2762 1384 268 1917 Fragments arranged by size: 2762 1917 1384 268 AfeI AGC'GCT Cuts at: 2803 2803 Size: 6331 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6134 AflIII A'CryG_T Cuts at: 1203 2968 3147 5230 6207 1203 Size: 1765 179 2083 977 1327 Fragments arranged by size: 2083 1765 1327 977 179 AgeI A'CCGG_T Cuts at: 2131 5162 2131 Size: 3031 3300 AhdI GACnn_n'nnGTC Cuts at: 5060 5060 Size: 6331 AleI CACnn'nnGTG Cuts at: 1166 3066 3348 1166 Size: 1900 282 4149 Fragments arranged by size: 4149 1900 282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2648 1184 Size: 1464 4867 AlwNI CAG_nnn'CTG Cuts at: 2879 3055 3137 4074 5646 2879 Size: 176 82 937 1572 3564 Fragments arranged by size: 3564 1572 937 176 82 ApaI G_GGCC'C Cuts at: 2682 3095 3234 2682 Size: 413 139 5779 Fragments arranged by size: 5779 413 139 ApaLI G'TGCA_C Cuts at: 2299 3563 4388 5544 2299 Size: 1264 825 1156 3086 Fragments arranged by size: 3086 1264 1156 825 ApoI r'AATT_y Cuts at: 1493 3640 3866 4018 6127 1493 Size: 2147 226 152 2109 1697 Fragments arranged by size: 2147 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3378 3378 Size: 6331 AseI AT'TA_AT Cuts at: 160 1032 4885 160 Size: 872 3853 1606 Fragments arranged by size: 3853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 AvaI C'yCGr_G Cuts at: 1870 1943 2452 2620 2674 2681 2763 2836 2929 Size: 73 509 168 54 7 82 73 93 Cuts at: 2929 3656 1870 Size: 727 4545 Fragments arranged by size: 4545 727 509 168 93 82 73 73 54 7 BamHI G'GATC_C Cuts at: 3661 4028 3661 Size: 367 5964 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2908 3100 3269 3277 3350 Size: 325 217 627 1121 192 169 8 73 Cuts at: 3350 3362 3454 3478 3652 5107 618 Size: 12 92 24 174 1455 1842 Fragments arranged by size: 1842 1455 1121 627 325 217 192 174 169 92 73 24 12 8 BanII G_rGCy'C Cuts at: 1391 2561 2682 2843 2861 3095 3234 3296 3650 Size: 1170 121 161 18 234 139 62 354 Cuts at: 3650 1391 Size: 4072 Fragments arranged by size: 4072 1170 354 234 161 139 121 62 18 BbeI G_GCGC'C Cuts at: 2912 3104 3273 3281 3366 3458 2912 Size: 192 169 8 85 92 5785 Fragments arranged by size: 5785 192 169 92 85 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2464 928 Size: 971 565 4795 Fragments arranged by size: 4795 971 565 BbvCI CC'TCA_GC Cuts at: 3392 3392 Size: 6331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2290 2324 4522 4556 1903 Size: 34 353 34 2198 34 3678 Fragments arranged by size: 3678 2198 353 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 5433 4223 Size: 1210 5121 BclI T'GATC_A Cuts at: 1548 1548 Size: 6331 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 2622 2814 Size: 107 122 71 631 605 442 508 192 Cuts at: 2814 2856 2906 3452 4942 136 Size: 42 50 546 1490 1525 Fragments arranged by size: 1525 1490 631 605 546 508 442 192 122 107 71 50 42 BglII A'GATC_T Cuts at: 6326 6326 Size: 6331 BlpI GC'TnA_GC Cuts at: 3299 3748 3299 Size: 449 5882 Bme1580I G_kGCm'C Cuts at: 1790 2037 2303 2682 3095 3234 3483 3567 4392 Size: 247 266 379 413 139 249 84 825 Cuts at: 4392 5548 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 825 413 379 266 249 247 139 84 BmgBI CAC'GTC Cuts at: 1318 3031 1318 Size: 1713 4618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4062 5020 442 Size: 416 53 83 399 24 2645 958 1753 Fragments arranged by size: 2645 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2850 1056 Size: 1794 4537 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 6331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2529 3020 4991 1239 Size: 1290 491 1971 2579 Fragments arranged by size: 2579 1971 1290 491 Bpu10I CC'TnA_GC Cuts at: 2713 3392 2713 Size: 679 5652 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 4456 5321 5619 2234 Size: 2222 865 298 2946 Fragments arranged by size: 2946 2222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4994 882 Size: 617 3495 2219 Fragments arranged by size: 3495 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4122 493 Size: 711 2918 2702 Fragments arranged by size: 2918 2702 711 BsaBI GATnn'nnATC Cuts at: 6325 6325 Size: 6331 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2093 2687 2909 Size: 53 83 186 936 551 9 594 222 Cuts at: 2909 2948 2994 3101 3168 3270 3278 3363 3455 Size: 39 46 107 67 102 8 85 92 Cuts at: 3455 4138 4520 5155 275 Size: 683 382 635 1451 Fragments arranged by size: 1451 936 683 635 594 551 382 222 186 107 102 92 85 83 67 53 46 39 9 8 BsaWI w'CCGG_w Cuts at: 2131 2531 2867 3619 4764 5162 5436 5583 5994 Size: 400 336 752 1145 398 274 147 411 Cuts at: 5994 2131 Size: 2468 Fragments arranged by size: 2468 1145 752 411 400 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2993 3023 1178 Size: 30 165 30 1590 30 4486 Fragments arranged by size: 4486 1590 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 2840 1186 Size: 1198 456 4677 Fragments arranged by size: 4677 1198 456 BseYI C'CCAG_C Cuts at: 2027 2917 3155 3289 3373 5534 6030 2027 Size: 890 238 134 84 2161 496 2328 Fragments arranged by size: 2328 2161 890 496 238 134 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3056 1163 Size: 573 1320 4438 Fragments arranged by size: 4438 1320 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2118 3084 3546 3781 4542 5570 Size: 275 186 117 966 462 235 761 1028 Cuts at: 5570 1540 Size: 2301 Fragments arranged by size: 2301 1028 966 761 462 275 235 186 117 BsiHKAI G_wGCw'C Cuts at: 2303 3567 4392 4477 5548 2303 Size: 1264 825 85 1071 3086 Fragments arranged by size: 3086 1264 1071 825 85 BsmI GAATG_Cn' Cuts at: 3842 3935 3842 Size: 93 6238 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2303 2561 2682 2843 2861 3095 Size: 399 247 266 258 121 161 18 234 Cuts at: 3095 3234 3296 3483 3567 3650 4392 4477 5548 Size: 139 62 187 84 83 742 85 1071 Cuts at: 5548 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 266 258 247 234 187 161 139 121 85 84 83 62 18 BspEI T'CCGG_A Cuts at: 3619 5994 3619 Size: 2375 3956 BspHI T'CATG_A Cuts at: 4220 5950 4220 Size: 1730 4601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 2771 3040 3619 3686 844 Size: 1485 442 269 579 67 3489 Fragments arranged by size: 3489 1485 579 442 269 67 BsrBI CCG'CTC Cuts at: 1394 1823 2513 3309 3385 3540 4218 1394 Size: 429 690 796 76 155 678 3507 Fragments arranged by size: 3507 796 690 678 429 155 76 BsrDI GCAATG_nn' Cuts at: 65 1248 4826 5000 65 Size: 1183 3578 174 1396 Fragments arranged by size: 3578 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2131 2641 2905 2985 3083 3266 3470 Size: 110 321 510 264 80 98 183 204 Cuts at: 3470 4975 5162 1700 Size: 1505 187 2869 Fragments arranged by size: 2869 1505 510 321 264 204 187 183 110 98 80 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6331 BssHII G'CGCG_C Cuts at: 1925 2214 2216 2381 3378 1925 Size: 289 2 165 997 4878 Fragments arranged by size: 4878 997 289 165 2 BssSI C'ACGA_G Cuts at: 4391 5403 4391 Size: 1012 5319 BstAPI GCAn_nnn'nTGC Cuts at: 794 3137 794 Size: 2343 3988 BstBI TT'CG_AA Cuts at: 5135 5135 Size: 6331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6331 BstXI CCAn_nnnn'nTGG Cuts at: 3068 3068 Size: 6331 BstYI r'GATC_y Cuts at: 1972 3661 4028 4413 4430 5882 5968 5980 6326 Size: 1689 367 385 17 1452 86 12 346 Cuts at: 6326 1972 Size: 1977 Fragments arranged by size: 1977 1689 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 2198 2230 2523 2936 3067 Size: 552 939 105 89 32 293 413 131 Cuts at: 3067 3120 3273 3417 3436 3504 513 Size: 53 153 144 19 68 3340 Fragments arranged by size: 3340 939 552 413 293 153 144 131 105 89 68 53 32 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2109 2293 2745 487 Size: 683 56 883 184 452 4073 Fragments arranged by size: 4073 883 683 452 184 56 BtsI GCAGTG_nn' Cuts at: 2406 3465 3847 4641 4661 2406 Size: 1059 382 794 20 4076 Fragments arranged by size: 4076 1059 794 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6296 DraI TTT'AAA Cuts at: 3635 3635 Size: 6331 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5338 809 Size: 507 4022 1802 Fragments arranged by size: 4022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2427 3081 Size: 54 1425 50 275 18 285 312 654 Cuts at: 3081 3264 3543 3778 4667 6296 8 Size: 183 279 235 889 1629 43 Fragments arranged by size: 1629 1425 889 654 312 285 279 275 235 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2115 3081 3543 3778 1537 Size: 275 303 966 462 235 4090 Fragments arranged by size: 4090 966 462 303 275 235 EarI CTCTTCn'nnn_ Cuts at: 1353 2883 4261 1353 Size: 1530 1378 3423 Fragments arranged by size: 3423 1530 1378 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2553 2937 3485 4918 5292 5438 Size: 1064 138 964 384 548 1433 374 146 Cuts at: 5438 387 Size: 1280 Fragments arranged by size: 1433 1280 1064 964 548 384 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2529 3020 4394 4991 5778 6046 1239 Size: 393 897 491 1374 597 787 268 1524 Fragments arranged by size: 1524 1374 897 787 597 491 393 268 EcoO109I rG'GnC_Cy Cuts at: 2504 2678 3073 3092 3200 3230 2504 Size: 174 395 19 108 30 5605 Fragments arranged by size: 5605 395 174 108 30 19 EcoRI G'AATT_C Cuts at: 3640 4018 3640 Size: 378 5953 EcoRV GAT'ATC Cuts at: 2959 2959 Size: 6331 FseI GG_CCGG'CC Cuts at: 3087 3087 Size: 6331 FspI TGC'GCA Cuts at: 2351 4837 2351 Size: 2486 3845 HaeII r_GCGC'y Cuts at: 1678 2320 2348 2426 2522 2805 2912 3104 3273 Size: 642 28 78 96 283 107 192 169 Cuts at: 3273 3281 3290 3366 3458 3618 5478 1678 Size: 8 9 76 92 160 1860 2531 Fragments arranged by size: 2531 1860 642 283 192 169 160 107 96 92 78 76 28 9 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2723 2755 4972 5004 5046 Size: 32 135 32 1151 32 2217 32 42 Cuts at: 5046 5078 1373 Size: 32 2626 Fragments arranged by size: 2626 2217 1151 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3166 3610 3675 3921 669 Size: 988 281 1228 444 65 246 3079 Fragments arranged by size: 3079 1228 988 444 281 246 65 HindIII A'AGCT_T Cuts at: 748 748 Size: 6331 HpaI GTT'AAC Cuts at: 3921 3921 Size: 6331 KasI G'GCGC_C Cuts at: 2908 3100 3269 3277 3362 3454 2908 Size: 192 169 8 85 92 5785 Fragments arranged by size: 5785 192 169 92 85 8 KpnI G_GTAC'C Cuts at: 3656 3656 Size: 6331 MfeI C'AATT_G Cuts at: 3930 3930 Size: 6331 MluI A'CGCG_T Cuts at: 2968 3147 6207 2968 Size: 179 3060 3092 Fragments arranged by size: 3092 3060 179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5445 5629 1305 Size: 4140 184 2007 Fragments arranged by size: 4140 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4509 Fragments arranged by size: 4509 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3066 3348 4289 4648 4807 518 Size: 648 1900 282 941 359 159 2042 Fragments arranged by size: 2042 1900 941 648 359 282 159 MspA1I CmG'CkG Cuts at: 1606 2111 2120 2200 2395 2525 2546 2632 2790 Size: 505 9 80 195 130 21 86 158 Cuts at: 2790 2944 2953 3137 3191 3220 3325 3373 3419 Size: 154 9 184 54 29 105 48 46 Cuts at: 3419 3438 3551 3747 4424 5572 5817 1606 Size: 19 113 196 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 505 245 196 195 184 158 154 130 113 105 86 80 54 48 46 29 21 19 9 9 NaeI GCC'GGC Cuts at: 2907 3085 3268 3472 2907 Size: 178 183 204 5766 Fragments arranged by size: 5766 204 183 178 NarI GG'CG_CC Cuts at: 2909 3101 3270 3278 3363 3455 2909 Size: 192 169 8 85 92 5785 Fragments arranged by size: 5785 192 169 92 85 8 NcoI C'CATG_G Cuts at: 513 1065 2004 2230 3273 3504 513 Size: 552 939 226 1043 231 3340 Fragments arranged by size: 3340 1043 939 552 231 226 NdeI CA'TA_TG Cuts at: 387 387 Size: 6331 NgoMIV G'CCGG_C Cuts at: 2905 3083 3266 3470 2905 Size: 178 183 204 5766 Fragments arranged by size: 5766 204 183 178 NheI G'CTAG_C Cuts at: 1052 2846 1052 Size: 1794 4537 NotI GC'GGCC_GC Cuts at: 2115 3778 2115 Size: 1663 4668 NspI r_CATG'y Cuts at: 1132 1229 3689 5234 1132 Size: 97 2460 1545 2229 Fragments arranged by size: 2460 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6331 PciI A'CATG_T Cuts at: 5230 5230 Size: 6331 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 5203 PfoI T'CCnGG_A Cuts at: 2997 2997 Size: 6331 PmeI GTTT'AAAC Cuts at: 3635 3635 Size: 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5913 5945 4400 Size: 32 1481 32 4786 Fragments arranged by size: 4786 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2504 2504 Size: 6331 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6331 PsiI TTA'TAA Cuts at: 3901 3901 Size: 6331 PspOMI G'GGCC_C Cuts at: 2678 3091 3230 2678 Size: 413 139 5779 Fragments arranged by size: 5779 413 139 PstI C_TGCA'G Cuts at: 830 2394 2610 3133 3142 3225 3330 3683 830 Size: 1564 216 523 9 83 105 353 3478 Fragments arranged by size: 3478 1564 523 353 216 105 83 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6331 PvuII CAG'CTG Cuts at: 2395 2546 2790 3137 2395 Size: 151 244 347 5589 Fragments arranged by size: 5589 347 244 151 SacII CC_GC'GG Cuts at: 2112 2201 2526 3420 3439 2112 Size: 89 325 894 19 5004 Fragments arranged by size: 5004 894 325 89 19 SalI G'TCGA_C Cuts at: 1936 3164 3673 1936 Size: 1228 509 4594 Fragments arranged by size: 4594 1228 509 SbfI CC_TGCA'GG Cuts at: 3683 3683 Size: 6331 ScaI AGT'ACT Cuts at: 4579 6291 4579 Size: 1712 4619 SfcI C'TryA_G Cuts at: 826 1045 2390 2606 3129 3138 3221 3326 3679 Size: 219 1345 216 523 9 83 105 353 Cuts at: 3679 4814 5495 5686 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1345 1135 681 523 353 219 216 191 105 83 9 SfoI GGC'GCC Cuts at: 2910 3102 3271 3279 3364 3456 2910 Size: 192 169 8 85 92 5785 Fragments arranged by size: 5785 192 169 92 85 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 SgrAI Cr'CCGG_yG Cuts at: 1700 2641 2905 2985 1700 Size: 941 264 80 5046 Fragments arranged by size: 5046 941 264 80 SmaI CCC'GGG Cuts at: 1872 2622 2683 2838 3658 1872 Size: 750 61 155 820 4545 Fragments arranged by size: 4545 820 750 155 61 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 4435 5336 5598 820 Size: 197 926 306 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4122 493 Size: 3629 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6331 SphI G_CATG'C Cuts at: 1132 3689 1132 Size: 2557 3774 SrfI GCCC'GGGC Cuts at: 2622 2838 2622 Size: 216 6115 SspI AAT'ATT Cuts at: 5 52 4255 5 Size: 47 4203 2081 Fragments arranged by size: 4203 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6331 StyI C'CwwG_G Cuts at: 513 1065 2004 2230 3273 3504 3770 513 Size: 552 939 226 1043 231 266 3074 Fragments arranged by size: 3074 1043 939 552 266 231 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3517 4358 4375 4528 4713 5158 Size: 1255 1533 404 841 17 153 185 445 Cuts at: 5158 325 Size: 1498 Fragments arranged by size: 1533 1498 1255 841 445 404 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4577 6289 96 Size: 274 80 33 51 1443 2600 1712 138 Fragments arranged by size: 2600 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 2958 2990 6291 6323 2273 Size: 32 653 32 3301 32 2281 Fragments arranged by size: 3301 2281 653 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2689 1316 Size: 1373 4958 XbaI T'CTAG_A Cuts at: 3667 3667 Size: 6331 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3472 3472 Size: 6331 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6331 XmaI C'CCGG_G Cuts at: 1870 2620 2681 2836 3656 1870 Size: 750 61 155 820 4545 Fragments arranged by size: 4545 820 750 155 61 XmnI GAAnn'nnTTC Cuts at: 4460 4460 Size: 6331 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2688 4139 276 Size: 53 83 186 936 1154 1451 2468 Fragments arranged by size: 2468 1451 1154 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI