(Linear) (Six-base) MAPSORT of: KIEE3425.seq Check: 9150 from: 1 to: 1626 KIEE3425.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 324 766 1035 1626 Size: 324 442 269 591 Fragments arranged by size: 591 442 324 269 AatII G_ACGT'C Cuts at: 0 685 1626 Size: 685 941 AccI GT'mk_AC Cuts at: 0 1160 1626 Size: 1160 466 AfeI AGC'GCT Cuts at: 0 798 1626 Size: 798 828 AflIII A'CryG_T Cuts at: 0 963 1142 1626 Size: 963 179 484 Fragments arranged by size: 963 484 179 AgeI A'CCGG_T Cuts at: 0 126 1626 Size: 126 1500 AleI CACnn'nnGTG Cuts at: 0 1061 1343 1626 Size: 1061 282 283 Fragments arranged by size: 1061 283 282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 643 1626 Size: 643 983 AlwNI CAG_nnn'CTG Cuts at: 0 874 1050 1132 1626 Size: 874 176 82 494 Fragments arranged by size: 874 494 176 82 ApaI G_GGCC'C Cuts at: 0 677 1090 1229 1626 Size: 677 413 139 397 Fragments arranged by size: 677 413 397 139 ApaLI G'TGCA_C Cuts at: 0 294 1558 1626 Size: 294 1264 68 Fragments arranged by size: 1264 294 68 AscI GG'CGCG_CC Cuts at: 0 1373 1626 Size: 1373 253 AvaI C'yCGr_G Cuts at: 0 447 615 669 676 758 831 924 1626 Size: 447 168 54 7 82 73 93 702 Fragments arranged by size: 702 447 168 93 82 73 54 7 BanI G'GyrC_C Cuts at: 0 903 1095 1264 1272 1345 1357 1449 1473 Size: 903 192 169 8 73 12 92 24 Cuts at: 1473 1626 Size: 153 Fragments arranged by size: 903 192 169 153 92 73 24 12 8 BanII G_rGCy'C Cuts at: 0 556 677 838 856 1090 1229 1291 1626 Size: 556 121 161 18 234 139 62 335 Fragments arranged by size: 556 335 234 161 139 121 62 18 BbeI G_GCGC'C Cuts at: 0 907 1099 1268 1276 1361 1453 1626 Size: 907 192 169 8 85 92 173 Fragments arranged by size: 907 192 173 169 92 85 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 459 1626 Size: 459 1167 BbvCI CC'TCA_GC Cuts at: 0 1387 1626 Size: 1387 239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 285 319 1626 Size: 285 34 1307 Fragments arranged by size: 1307 285 34 BglI GCCn_nnn'nGGC Cuts at: 0 109 617 809 851 901 1447 1626 Size: 109 508 192 42 50 546 179 Fragments arranged by size: 546 508 192 179 109 50 42 BlpI GC'TnA_GC Cuts at: 0 1294 1626 Size: 1294 332 Bme1580I G_kGCm'C Cuts at: 0 32 298 677 1090 1229 1478 1562 1626 Size: 32 266 379 413 139 249 84 64 Fragments arranged by size: 413 379 266 249 139 84 64 32 BmgBI CAC'GTC Cuts at: 0 1026 1626 Size: 1026 600 BmtI G_CTAG'C Cuts at: 0 845 1626 Size: 845 781 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 27 1626 Size: 27 1599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1015 1626 Size: 524 491 611 Fragments arranged by size: 611 524 491 Bpu10I CC'TnA_GC Cuts at: 0 708 1387 1626 Size: 708 679 239 Fragments arranged by size: 708 679 239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 1626 Size: 229 1397 BsaHI Gr'CG_yC Cuts at: 0 79 88 682 904 943 989 1096 1163 Size: 79 9 594 222 39 46 107 67 Cuts at: 1163 1265 1273 1358 1450 1626 Size: 102 8 85 92 176 Fragments arranged by size: 594 222 176 107 102 92 85 79 67 46 39 9 8 BsaWI w'CCGG_w Cuts at: 0 126 526 862 1614 1626 Size: 126 400 336 752 12 Fragments arranged by size: 752 400 336 126 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 988 1018 1626 Size: 988 30 608 Fragments arranged by size: 988 608 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 379 835 1626 Size: 379 456 791 Fragments arranged by size: 791 456 379 BseYI C'CCAG_C Cuts at: 0 22 912 1150 1284 1368 1626 Size: 22 890 238 134 84 258 Fragments arranged by size: 890 258 238 134 84 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1626 Size: 1051 575 BsiEI CG_ry'CG Cuts at: 0 113 1079 1541 1626 Size: 113 966 462 85 Fragments arranged by size: 966 462 113 85 BsiHKAI G_wGCw'C Cuts at: 0 298 1562 1626 Size: 298 1264 64 Fragments arranged by size: 1264 298 64 Bsp1286I G_dGCh'C Cuts at: 0 32 298 556 677 838 856 1090 1229 Size: 32 266 258 121 161 18 234 139 Cuts at: 1229 1291 1478 1562 1626 Size: 62 187 84 64 Fragments arranged by size: 266 258 234 187 161 139 121 84 64 62 32 18 BspEI T'CCGG_A Cuts at: 0 1614 1626 Size: 1614 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 324 766 1035 1626 Size: 324 442 269 591 Fragments arranged by size: 591 442 324 269 BsrBI CCG'CTC Cuts at: 0 508 1304 1380 1535 1626 Size: 508 796 76 155 91 Fragments arranged by size: 796 508 155 91 76 BsrFI r'CCGG_y Cuts at: 0 126 636 900 980 1078 1261 1465 1626 Size: 126 510 264 80 98 183 204 161 Fragments arranged by size: 510 264 204 183 161 126 98 80 BssHII G'CGCG_C Cuts at: 0 209 211 376 1373 1626 Size: 209 2 165 997 253 Fragments arranged by size: 997 253 209 165 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1132 1626 Size: 1132 494 BstXI CCAn_nnnn'nTGG Cuts at: 0 1063 1626 Size: 1063 563 BtgI C'CryG_G Cuts at: 0 104 193 225 518 931 1062 1115 1268 Size: 104 89 32 293 413 131 53 153 Cuts at: 1268 1412 1431 1499 1626 Size: 144 19 68 127 Fragments arranged by size: 413 293 153 144 131 127 104 89 68 53 32 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 104 288 740 1626 Size: 104 184 452 886 Fragments arranged by size: 886 452 184 104 BtsI GCAGTG_nn' Cuts at: 0 401 1460 1626 Size: 401 1059 166 Fragments arranged by size: 1059 401 166 EaeI y'GGCC_r Cuts at: 0 110 422 1076 1259 1538 1626 Size: 110 312 654 183 279 88 Fragments arranged by size: 654 312 279 183 110 88 EagI C'GGCC_G Cuts at: 0 110 1076 1538 1626 Size: 110 966 462 88 Fragments arranged by size: 966 462 110 88 EarI CTCTTCn'nnn_ Cuts at: 0 878 1626 Size: 878 748 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 548 932 1480 1626 Size: 548 384 548 146 Fragments arranged by size: 548 548 384 146 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1015 1626 Size: 524 491 611 Fragments arranged by size: 611 524 491 EcoO109I rG'GnC_Cy Cuts at: 0 499 673 1068 1087 1195 1225 1626 Size: 499 174 395 19 108 30 401 Fragments arranged by size: 499 401 395 174 108 30 19 EcoRV GAT'ATC Cuts at: 0 954 1626 Size: 954 672 FseI GG_CCGG'CC Cuts at: 0 1082 1626 Size: 1082 544 FspI TGC'GCA Cuts at: 0 346 1626 Size: 346 1280 HaeII r_GCGC'y Cuts at: 0 315 343 421 517 800 907 1099 1268 Size: 315 28 78 96 283 107 192 169 Cuts at: 1268 1276 1285 1361 1453 1613 1626 Size: 8 9 76 92 160 13 Fragments arranged by size: 315 283 192 169 160 107 96 92 78 76 28 13 9 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 718 750 1626 Size: 718 32 876 Fragments arranged by size: 876 718 32 HincII GTy'rAC Cuts at: 0 1161 1605 1626 Size: 1161 444 21 Fragments arranged by size: 1161 444 21 KasI G'GCGC_C Cuts at: 0 903 1095 1264 1272 1357 1449 1626 Size: 903 192 169 8 85 92 177 Fragments arranged by size: 903 192 177 169 92 85 8 MluI A'CGCG_T Cuts at: 0 963 1142 1626 Size: 963 179 484 Fragments arranged by size: 963 484 179 MslI CAynn'nnrTG Cuts at: 0 1061 1343 1626 Size: 1061 282 283 Fragments arranged by size: 1061 283 282 MspA1I CmG'CkG Cuts at: 0 106 115 195 390 520 541 627 785 Size: 106 9 80 195 130 21 86 158 Cuts at: 785 939 948 1132 1186 1215 1320 1368 1414 Size: 154 9 184 54 29 105 48 46 Cuts at: 1414 1433 1546 1626 Size: 19 113 80 Fragments arranged by size: 195 184 158 154 130 113 106 105 86 80 80 54 48 46 29 21 19 9 9 NaeI GCC'GGC Cuts at: 0 902 1080 1263 1467 1626 Size: 902 178 183 204 159 Fragments arranged by size: 902 204 183 178 159 NarI GG'CG_CC Cuts at: 0 904 1096 1265 1273 1358 1450 1626 Size: 904 192 169 8 85 92 176 Fragments arranged by size: 904 192 176 169 92 85 8 NcoI C'CATG_G Cuts at: 0 225 1268 1499 1626 Size: 225 1043 231 127 Fragments arranged by size: 1043 231 225 127 NgoMIV G'CCGG_C Cuts at: 0 900 1078 1261 1465 1626 Size: 900 178 183 204 161 Fragments arranged by size: 900 204 183 178 161 NheI G'CTAG_C Cuts at: 0 841 1626 Size: 841 785 NotI GC'GGCC_GC Cuts at: 0 110 1626 Size: 110 1516 PflMI CCAn_nnn'nTGG Cuts at: 0 231 1626 Size: 231 1395 PfoI T'CCnGG_A Cuts at: 0 992 1626 Size: 992 634 PpuMI rG'GwC_Cy Cuts at: 0 499 1626 Size: 499 1127 PspOMI G'GGCC_C Cuts at: 0 673 1086 1225 1626 Size: 673 413 139 401 Fragments arranged by size: 673 413 401 139 PstI C_TGCA'G Cuts at: 0 389 605 1128 1137 1220 1325 1626 Size: 389 216 523 9 83 105 301 Fragments arranged by size: 523 389 301 216 105 83 9 PvuII CAG'CTG Cuts at: 0 390 541 785 1132 1626 Size: 390 151 244 347 494 Fragments arranged by size: 494 390 347 244 151 SacII CC_GC'GG Cuts at: 0 107 196 521 1415 1434 1626 Size: 107 89 325 894 19 192 Fragments arranged by size: 894 325 192 107 89 19 SalI G'TCGA_C Cuts at: 0 1159 1626 Size: 1159 467 SfcI C'TryA_G Cuts at: 0 385 601 1124 1133 1216 1321 1626 Size: 385 216 523 9 83 105 305 Fragments arranged by size: 523 385 305 216 105 83 9 SfoI GGC'GCC Cuts at: 0 905 1097 1266 1274 1359 1451 1626 Size: 905 192 169 8 85 92 175 Fragments arranged by size: 905 192 175 169 92 85 8 SgrAI Cr'CCGG_yG Cuts at: 0 636 900 980 1626 Size: 636 264 80 646 Fragments arranged by size: 646 636 264 80 SmaI CCC'GGG Cuts at: 0 617 678 833 1626 Size: 617 61 155 793 Fragments arranged by size: 793 617 155 61 SmlI C'TyrA_G Cuts at: 0 244 1626 Size: 244 1382 SrfI GCCC'GGGC Cuts at: 0 617 833 1626 Size: 617 216 793 Fragments arranged by size: 793 617 216 StyI C'CwwG_G Cuts at: 0 225 1268 1499 1626 Size: 225 1043 231 127 Fragments arranged by size: 1043 231 225 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1108 1512 1626 Size: 1108 404 114 Fragments arranged by size: 1108 404 114 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 268 300 953 985 1626 Size: 268 32 653 32 641 Fragments arranged by size: 653 641 268 32 32 Tth111I GACn'n_nGTC Cuts at: 0 684 1626 Size: 684 942 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1467 1626 Size: 1467 159 XmaI C'CCGG_G Cuts at: 0 615 676 831 1626 Size: 615 61 155 795 Fragments arranged by size: 795 615 155 61 ZraI GAC'GTC Cuts at: 0 683 1626 Size: 683 943 Enzymes that do cut: AarI AatII AccI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV FseI FspI HaeII Hin4I HincII KasI MluI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SalI SfcI SfoI SgrAI SmaI SmlI SrfI StyI TaqII TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AcuI AflII AhdI AloI ApoI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmrI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI FalI FspAI HindIII HpaI KpnI MfeI MmeI MscI NdeI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI SwaI TatI XbaI XhoI XmnI