Restriction map for
pFN21AE3100
##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AcsI Aor51HI ApoI AscI
AseI AsnI Asp700I BamHI BglII
BsiWI BspDI BspHI BspLU11I BsrBI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
HincII HindII HindIII HpaI KspI
MluI NdeI NheI NruI NsiI
PacI PaeR7I PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SnaBI Sse8387I SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 838 GACGTC GACGT^C
AflII 2503 CTTAAG C^TTAAG
AhdI 2358 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1903 GTGCAC G^TGCAC
AocI 1474 CCTCAGG CC^T[ACGT]AGG
ApaLI 1903 GTGCAC G^TGCAC
AspEI 2358 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4461 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 976 TGCGCA TGC^GCA
AvrII 2646 CCTAGG C^CTAGG
BbrPI 178 CACGTG CAC^GTG
BbvCI 2177 CCTCAGC CCTCAGC(-5/-2)
BcgI 5753 GCAAGATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2503 CTTAAG C^TTAAG
BlnI 2646 CCTAGG C^CTAGG
BlpI 3657 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3657 GCTGAGC GC^T[ACGT]AGC
BsaBI 3438 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3924 GAATGC GAATGC(1/-1)
BstBI 3225 TTCGAA TT^CGAA
Bsu36I 1474 CCTCAGG CC^T[ACGT]AGG
CelII 3657 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2358 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1474 CCTCAGG CC^T[ACGT]AGG
EcoNI 1341 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 3564 GGCCGGCC GGCCGG^CC
FspI 976 TGCGCA TGC^GCA
MamI 3438 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5190 CAATTG C^AATTG
MunI 5190 CAATTG C^AATTG
NotI 1654 GCGGCCGC GC^GGCCGC
NspI 1823 GCATGC [GA]CATG^[CT]
NspV 3225 TTCGAA TT^CGAA
PflFI 4461 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 178 CACGTG CAC^GTG
PmlI 178 CACGTG CAC^GTG
PshAI 3855 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1992 GAAGAGC GCTCTTC(1/4)
ScaI 5765 AGTACT AGT^ACT
SexAI 1046 ACCAGGT A^CC[AT]GGT
SfuI 3225 TTCGAA TT^CGAA
SgrAI 4944 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2704 ACTAGT A^CTAGT
SphI 1823 GCATGC GCATG^C
StuI 1034 AGGCCT AGG^CCT
TfiI 5603 GAATC G^A[AT]TC
Tth111I 4461 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 3440 TCCGGA T^CCGGA
5706 TCCGGA
AgeI 1468 ACCGGT A^CCGGT
4473 ACCGGT
BbsI 1731 GAAGAC GAAGAC(2/6)
1830 GAAGAC
BciVI 903 GGATAC GTATCC(6/5)
933 GGATAC
BclI 2768 TGATCA T^GATCA
2885 TGATCA
BpuAI 1731 GAAGAC GAAGAC(2/6)
1830 GAAGAC
BsaAI 178 CACGTG [CT]AC^GT[GA]
802 TACGTG
BseAI 3440 TCCGGA T^CCGGA
5706 TCCGGA
BseRI 1788 CTCCTC GAGGAG(10/8)
4828 GAGGAG
Bsp1407I 182 TGTACA T^GTACA
1001 TGTACA
BspEI 3440 TCCGGA T^CCGGA
5706 TCCGGA
BsrGI 182 TGTACA T^GTACA
1001 TGTACA
BstYI 1673 AGATCC [GA]^GATC[CT]
4641 GGATCT
DrdI 4155 GACCGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4419 GACAACCGTGTC
FbaI 2768 TGATCA T^GATCA
2885 TGATCA
MflI 1673 AGATCC [GA]^GATC[CT]
4641 GGATCT
MroI 3440 TCCGGA T^CCGGA
5706 TCCGGA
PinAI 1468 ACCGGT A^CCGGT
4473 ACCGGT
SspBI 182 TGTACA T^GTACA
1001 TGTACA
XhoII 1673 AGATCC [GA]^GATC[CT]
4641 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI