################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI Fnu4HI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI MwoI NarI NciI NcoI NdeI NdeII NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AcsI 256 GAATTC [GA]^AATT[CT] ApoI 256 GAATTC [GA]^AATT[CT] AvaI 215 CCCGAG C^[CT]CG[GA]G BfaI 151 CTAG C^TAG BpmI 188 CTCCAG CTGGAG(16/14) BsaI 79 GAGACC GGTCTC(1/5) BsmAI 79 GAGAC GTCTC(1/5) BsoBI 215 CCCGAG C^[CT]CG[GA]G BsrFI 19 GCCGGC [GA]^CCGG[CT] BstXI 146 CCATACTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 19 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 19 GCCGGC [GA]^CCGG[CT] DraI 175 TTTAAA TTT^AAA EarI 30 GAAGAG CTCTTC(1/4) EcoRI 256 GAATTC G^AATTC HaeIII 60 GGCC GG^CC HgaI 38 GCGTC GACGC(5/10) HphI 27 GGTGA GGTGA(8/7) Ksp632I 30 GAAGAG CTCTTC(1/4) MaeI 151 CTAG C^TAG MboII 30 GAAGA GAAGA(8/7) MseI 176 TTAA T^TAA MslI 147 CATACTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 19 GCCGGC GCC^GGC NgoMIV 19 GCCGGC G^CCGGC NlaIII 261 CATG CATG^ SfaNI 72 GATGC GCATC(5/9) SfcI 162 CTACAG C^T[GA][CT]AG SpeI 150 ACTAGT A^CTAGT TaqI 142 TCGA T^CGA Tru9I 176 TTAA T^TAA Tsp509I 257 AATT ^AATT TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 142 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AciI 103 CCGC CCGC(-3/-1) 23 GCGG BsmFI 92 GGGAC GGGAC(10/14) 40 GTCCC BspMI 108 ACCTGC ACCTGC(4/8) 282 ACCTGC BsrI 88 ACTGG ACTGG(1/-1) 14 CCAGT DdeI 96 CTCAG C^T[ACGT]AG 134 CTGAG FauI 102 CCCGC CCCGC(4/6) 23 GCGGG HapII 20 CCGG C^CGG 76 CCGG HpaII 20 CCGG C^CGG 76 CCGG MspI 20 CCGG C^CGG 76 CCGG PleI 94 GACTC GAGTC(4/5) 136 GAGTC TfiI 49 GATTC G^A[AT]TC 227 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BseRI BsiYI BslI HinfI MnlI