Restriction map for
pFN21AE3710
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HinfI HpaI KpnI KspI
MaeI MamI MboI MfeI MflI
MluI MunI NaeI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsaI
RsrII SacI SacII Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 55 GTCGAC GT^[AC][GT]AC
AccIII 9 TCCGGA T^CCGGA
AclI 307 AACGTT AA^CGTT
AcsI 811 GAATTT [GA]^AATT[CT]
AlwNI 439 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 811 GAATTT [GA]^AATT[CT]
AseI 585 ATTAAT AT^TAAT
AsnI 585 ATTAAT AT^TAAT
AvaI 280 CTCGAG C^[CT]CG[GA]G
BanII 191 GAGCCC G[GA]GC[CT]^C
BbvCI 646 GCTGAGG CCTCAGC(-5/-2)
BlpI 42 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC
BsaWI 9 TCCGGA [AT]^CCGG[AT]
BseAI 9 TCCGGA T^CCGGA
BsgI 532 GTGCAG GTGCAG(16/14)
BsmAI 570 GAGAC GTCTC(1/5)
BsmFI 701 GGGAC GGGAC(10/14)
BsmI 84 GAATGC GAATGC(1/-1)
BsoBI 280 CTCGAG C^[CT]CG[GA]G
BspEI 9 TCCGGA T^CCGGA
BsrDI 428 CATTGC GCAATG(2/0)
BstEII 636 GGTAACC G^GT[ACGT]ACC
BstPI 636 GGTAACC G^GT[ACGT]ACC
CelII 42 GCTCAGC GC^T[ACGT]AGC
EcoNI 148 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 636 GGTAACC G^GT[ACGT]ACC
EcoT14I 195 CCAAGG C^C[AT][AT]GG
HincII 55 GTCGAC GT[CT]^[GA]AC
HindII 55 GTCGAC GT[CT]^[GA]AC
HphI 422 TCACC GGTGA(8/7)
MroI 9 TCCGGA T^CCGGA
NspI 599 ACATGC [GA]CATG^[CT]
PaeR7I 280 CTCGAG C^TCGAG
PflMI 793 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 585 ATTAAT AT^TAAT
Psp1406I 307 AACGTT AA^CGTT
SalI 55 GTCGAC G^TCGAC
SapI 554 GAAGAGC GCTCTTC(1/4)
SfaNI 133 GATGC GCATC(5/9)
SmlI 280 CTCGAG C^T[CT][GA]AG
StyI 195 CCAAGG C^C[AT][AT]GG
Tsp45I 517 GTGAC ^GT[GC]AC
Tsp509I 812 AATT ^AATT
Van91I 793 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 804 CCAGGCTGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 280 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 244 GGCGCC G[GA]^CG[CT]C
367 GGCGCC
AvaII 12 GGACC G^G[AT]CC
702 GGACC
BalI 258 TGGCCA TGG^CCA
801 TGGCCA
BbeI 244 GGCGCC GGCGC^C
367 GGCGCC
BglI 359 GCCCACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
652 GCCATAACGGC
BmyI 191 GAGCCC G[AGT]GC[ACT]^C
285 GGGCAC
BpmI 382 CTGGAG CTGGAG(16/14)
445 CTGGAG
BsaHI 244 GGCGCC G[GA]^CG[CT]C
367 GGCGCC
BsaJI 195 CCAAGG C^C[ACGT][ACGT]GG
340 CCTGGG
Bsp1286I 191 GAGCCC G[AGT]GC[ACT]^C
285 GGGCAC
BstF5I 132 GGATG GGATG(2/0)
680 GGATG
EaeI 258 TGGCCA [CT]^GGCC[GA]
801 TGGCCA
FauI 796 CCCGC CCCGC(4/6)
677 GCGGG
FokI 132 GGATG GGATG(9/13)
680 GGATG
HaeII 244 GGCGCC [GA]GCGC^[CT]
367 GGCGCC
HapII 10 CCGG C^CGG
248 CCGG
Hin1I 244 GGCGCC G[GA]^CG[CT]C
367 GGCGCC
HpaII 10 CCGG C^CGG
248 CCGG
KasI 244 GGCGCC G^GCGCC
367 GGCGCC
MaeII 308 ACGT A^CGT
410 ACGT
MaeIII 517 GTGAC ^GT[ACGT]AC
637 GTAAC
MluNI 258 TGGCCA TGG^CCA
801 TGGCCA
MscI 258 TGGCCA TGG^CCA
801 TGGCCA
MseI 178 TTAA T^TAA
586 TTAA
MslI 510 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
612 CATGGCAGTG
MspI 10 CCGG C^CGG
248 CCGG
NarI 244 GGCGCC GG^CGCC
367 GGCGCC
PstI 50 CTGCAG CTGCA^G
436 CTGCAG
PvuII 433 CAGCTG CAG^CTG
644 CAGCTG
SfoI 244 GGCGCC GGC^GCC
367 GGCGCC
TaqI 56 TCGA T^CGA
281 TCGA
Tru9I 178 TTAA T^TAA
586 TTAA
TthHB8I 56 TCGA T^CGA
281 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BseRI BsiYI BslI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EarI EcoRII Fnu4HI HaeIII
HhaI HinP1I ItaI Ksp632I MboII
MnlI MspA1I MvaI MvnI MwoI
NlaIII NlaIV Sau96I ScrFI SfcI
TseI TspRI