Restriction map for
pFN21AE3710
(Circular) (Six-base) MAPSORT of: pFN21AE3710.seq Check: 819 from: 1 to: 5569
pFN21AE3710 5569 bp
With 182 enzymes: SgfI *
March 28, 2012 12:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 2912 1937
Size: 124 851 4594
Fragments arranged by size:
4594 851 124
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 2313 3696 4069 1563
Size: 750 1383 373 3063
Fragments arranged by size:
3063 1383 750 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 3632 5016 5284 1632
Size: 501 1499 1384 268 1917
Fragments arranged by size:
1917 1499 1384 501 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2449 3312 4884 2449
Size: 863 1572 3134
Fragments arranged by size:
3134 1572 863
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2816 2878 3104 3256 5365 1493
Size: 1323 62 226 152 2109 1697
Fragments arranged by size:
2109 1697 1323 226 152 62
AseI AT'TA_AT
Cuts at: 160 1032 2591 4123 160
Size: 872 1559 1532 1606
Fragments arranged by size:
1606 1559 1532 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2285 2894 1870
Size: 73 342 609 4545
Fragments arranged by size:
4545 609 342 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2291 2372 2665 2890
Size: 325 217 627 462 42 81 293 225
Cuts at: 2890 4345 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 462 325 293 225 217
81 42
BanII G_rGCy'C
Cuts at: 1391 2200 2888 1391
Size: 809 688 4072
Fragments arranged by size:
4072 809 688
BbeI G_GCGC'C
Cuts at: 2253 2376 2253
Size: 123 5446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4598
BbvCI CC'TCA_GC
Cuts at: 2652 2652
Size: 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2370 2663 4180
Size: 107 122 71 631 605 698 293 1517
Cuts at: 4180 136
Size: 1525
Fragments arranged by size:
1525 1517 698 631 605 293 122 107
71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2048 2986 2048
Size: 938 4631
Bme1580I G_kGCm'C
Cuts at: 1790 2294 3630 4786 1790
Size: 504 1336 1156 2573
Fragments arranged by size:
2573 1336 1156 504
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2475 3300 4258
Size: 416 53 83 399 24 1058 825 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 1058 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2471 4229 1239
Size: 1169 63 1758 2579
Fragments arranged by size:
2579 1758 1169 63
Bpu10I CC'TnA_GC
Cuts at: 2148 2154 2652 2148
Size: 6 498 5065
Fragments arranged by size:
5065 498 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2373 3376 3758
Size: 53 83 186 936 717 123 1003 382
Cuts at: 3758 4393 275
Size: 635 1451
Fragments arranged by size:
1451 1003 936 717 635 382 186 123
83 53
BsaWI w'CCGG_w
Cuts at: 2014 4002 4400 4674 4821 5232 2014
Size: 1988 398 274 147 411 2351
Fragments arranged by size:
2351 1988 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 1178
Size: 30 165 30 1035 30 4279
Fragments arranged by size:
4279 1035 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2587 2745 1186
Size: 955 446 158 4010
Fragments arranged by size:
4010 955 446 158
BseYI C'CCAG_C
Cuts at: 4772 5268 4772
Size: 496 5073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2558 1163
Size: 573 822 4174
Fragments arranged by size:
4174 822 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3630 3715 4786 3630
Size: 85 1071 4413
Fragments arranged by size:
4413 1071 85
BsmI GAATG_Cn'
Cuts at: 2095 3080 3173 2095
Size: 985 93 4491
Fragments arranged by size:
4491 985 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2294 2888 3630 3715 4786 1391
Size: 399 410 94 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 410 399 94 85
BspEI T'CCGG_A
Cuts at: 2014 5232 2014
Size: 3218 2351
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2924 844
Size: 2080 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2432 4064 4238 65
Size: 1183 1184 1632 174 1396
Fragments arranged by size:
1632 1396 1184 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2641 1184
Size: 1457 4112
BstYI r'GATC_y
Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564
Size: 927 367 385 17 1452 86 12 346
Cuts at: 5564 1972
Size: 1977
Fragments arranged by size:
1977 1452 927 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2319 487
Size: 683 56 1093 3737
Fragments arranged by size:
3737 1093 683 56
BtsI GCAGTG_nn'
Cuts at: 2541 2628 3085 3879 3899 2541
Size: 87 457 794 20 4211
Fragments arranged by size:
4211 794 457 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 2806 3016
Size: 54 1425 50 275 18 433 543 210
Cuts at: 3016 3905 5534 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 543 433 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2127 2554 3499 1353
Size: 756 18 427 945 3423
Fragments arranged by size:
3423 945 756 427 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2220 2241 4156 4530 4676 387
Size: 1064 138 631 21 1915 374 146 1280
Fragments arranged by size:
1915 1280 1064 631 374 146 138 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2408 2471 3632 4229 5016 5284
Size: 393 501 275 63 1161 597 787 268
Cuts at: 5284 1239
Size: 1524
Fragments arranged by size:
1524 1161 787 597 501 393 275 268
63
EcoNI CCTnn'n_nnAGG
Cuts at: 2157 2157
Size: 5569
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2253 2376 4716 1678
Size: 575 123 2340 2531
Fragments arranged by size:
2531 2340 575 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2563 2595 4210 4242 4284
Size: 32 135 32 991 32 1615 32 42
Cuts at: 4284 4316 1373
Size: 32 2626
Fragments arranged by size:
2626 1615 991 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2062 2913 3159 669
Size: 988 281 124 851 246 3079
Fragments arranged by size:
3079 988 851 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KasI G'GCGC_C
Cuts at: 2249 2372 2249
Size: 123 5446
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2488 4683 4867 1305
Size: 1183 2195 184 2007
Fragments arranged by size:
2195 2007 1183 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2265 2808 10
Size: 54 1425 343 433 543 2771
Fragments arranged by size:
2771 1425 543 433 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2621 3527 3886 4045 518
Size: 648 1353 102 906 359 159 2042
Fragments arranged by size:
2042 1353 906 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2298 2440 2651 2682 2804 2985 3662 4810
Size: 692 142 211 31 122 181 677 1148
Cuts at: 4810 5055 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 692 677 245 211 181 142
122 31
NarI GG'CG_CC
Cuts at: 2250 2373 2250
Size: 123 5446
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2608 2927 4472 1132
Size: 97 1379 319 1545 2229
Fragments arranged by size:
2229 1545 1379 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2804 1108
Size: 1696 3873
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PspXI vC'TCGA_Gb
Cuts at: 2285 2285
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2059 2445 2921 830
Size: 1229 386 476 3478
Fragments arranged by size:
3478 1229 476 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2440 2651 2440
Size: 211 5358
SalI G'TCGA_C
Cuts at: 1936 2060 2911 1936
Size: 124 851 4594
Fragments arranged by size:
4594 851 124
SapI GCTCTTCn'nnn_
Cuts at: 2554 2554
Size: 5569
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2055 2078 2441 2917 4052 4733 4924
Size: 219 1010 23 363 476 1135 681 191
Cuts at: 4924 826
Size: 1471
Fragments arranged by size:
1471 1135 1010 681 476 363 219 191
23
SfoI GGC'GCC
Cuts at: 2251 2374 2251
Size: 123 5446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 3673 4574 4836 820
Size: 197 926 342 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 342 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2200 3008 513
Size: 552 939 196 808 3074
Fragments arranged by size:
3074 939 808 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3596 3613 3766 3951 4396 325
Size: 1255 2016 17 153 185 445 1498
Fragments arranged by size:
2016 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 2702 2734 5529 5561 2438
Size: 32 232 32 2795 32 2446
Fragments arranged by size:
2795 2446 232 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2816 2816
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 2285 1943
Size: 342 5227
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI