Restriction map for
pFN21AE3710
(Linear) (Six-base) MAPSORT of: KIEE3710.seq Check: 1653 from: 1 to: 864
KIEE3710.orf
With 182 enzymes: SgfI *
March 22, 2012 16:41 ..
AccI GT'mk_AC
Cuts at: 0 56 864
Size: 56 808
AclI AA'CG_TT
Cuts at: 0 308 864
Size: 308 556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 864
Size: 128 736
AlwNI CAG_nnn'CTG
Cuts at: 0 444 864
Size: 444 420
ApoI r'AATT_y
Cuts at: 0 811 864
Size: 811 53
AseI AT'TA_AT
Cuts at: 0 586 864
Size: 586 278
AvaI C'yCGr_G
Cuts at: 0 280 864
Size: 280 584
BanI G'GyrC_C
Cuts at: 0 244 286 367 660 864
Size: 244 42 81 293 204
Fragments arranged by size:
293 244 204 81 42
BanII G_rGCy'C
Cuts at: 0 195 864
Size: 195 669
BbeI G_GCGC'C
Cuts at: 0 248 371 864
Size: 248 123 493
Fragments arranged by size:
493 248 123
BbvCI CC'TCA_GC
Cuts at: 0 647 864
Size: 647 217
BglI GCCn_nnn'nGGC
Cuts at: 0 365 658 864
Size: 365 293 206
Fragments arranged by size:
365 293 206
BlpI GC'TnA_GC
Cuts at: 0 43 864
Size: 43 821
Bme1580I G_kGCm'C
Cuts at: 0 289 864
Size: 289 575
BmrI ACTGGGnnnn_n'
Cuts at: 0 470 864
Size: 470 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 466 864
Size: 403 63 398
Fragments arranged by size:
403 398 63
Bpu10I CC'TnA_GC
Cuts at: 0 143 149 647 864
Size: 143 6 498 217
Fragments arranged by size:
498 217 143 6
BsaHI Gr'CG_yC
Cuts at: 0 245 368 864
Size: 245 123 496
Fragments arranged by size:
496 245 123
BsaWI w'CCGG_w
Cuts at: 0 9 864
Size: 9 855
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 864
Size: 433 30 401
Fragments arranged by size:
433 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 582 740 864
Size: 136 446 158 124
Fragments arranged by size:
446 158 136 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 864
Size: 553 311
BsmI GAATG_Cn'
Cuts at: 0 90 864
Size: 90 774
Bsp1286I G_dGCh'C
Cuts at: 0 195 289 864
Size: 195 94 575
Fragments arranged by size:
575 195 94
BspEI T'CCGG_A
Cuts at: 0 9 864
Size: 9 855
BsrDI GCAATG_nn'
Cuts at: 0 427 864
Size: 427 437
BstEII G'GTnAC_C
Cuts at: 0 636 864
Size: 636 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 314 864
Size: 314 550
BtsI GCAGTG_nn'
Cuts at: 0 536 623 864
Size: 536 87 241
Fragments arranged by size:
536 241 87
EaeI y'GGCC_r
Cuts at: 0 258 801 864
Size: 258 543 63
Fragments arranged by size:
543 258 63
EarI CTCTTCn'nnn_
Cuts at: 0 104 122 549 864
Size: 104 18 427 315
Fragments arranged by size:
427 315 104 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 215 236 864
Size: 215 21 628
Fragments arranged by size:
628 215 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 403 466 864
Size: 128 275 63 398
Fragments arranged by size:
398 275 128 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 152 864
Size: 152 712
HaeII r_GCGC'y
Cuts at: 0 248 371 864
Size: 248 123 493
Fragments arranged by size:
493 248 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 864
Size: 558 32 274
Fragments arranged by size:
558 274 32
HincII GTy'rAC
Cuts at: 0 57 864
Size: 57 807
KasI G'GCGC_C
Cuts at: 0 244 367 864
Size: 244 123 497
Fragments arranged by size:
497 244 123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 864
Size: 483 381
MscI TGG'CCA
Cuts at: 0 260 803 864
Size: 260 543 61
Fragments arranged by size:
543 260 61
MslI CAynn'nnrTG
Cuts at: 0 514 616 864
Size: 514 102 248
Fragments arranged by size:
514 248 102
MspA1I CmG'CkG
Cuts at: 0 293 435 646 677 799 864
Size: 293 142 211 31 122 65
Fragments arranged by size:
293 211 142 122 65 31
NarI GG'CG_CC
Cuts at: 0 245 368 864
Size: 245 123 496
Fragments arranged by size:
496 245 123
NspI r_CATG'y
Cuts at: 0 603 864
Size: 603 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 799 864
Size: 799 65
PspXI vC'TCGA_Gb
Cuts at: 0 280 864
Size: 280 584
PstI C_TGCA'G
Cuts at: 0 54 440 864
Size: 54 386 424
Fragments arranged by size:
424 386 54
PvuII CAG'CTG
Cuts at: 0 435 646 864
Size: 435 211 218
Fragments arranged by size:
435 218 211
SalI G'TCGA_C
Cuts at: 0 55 864
Size: 55 809
SapI GCTCTTCn'nnn_
Cuts at: 0 549 864
Size: 549 315
SfcI C'TryA_G
Cuts at: 0 50 73 436 864
Size: 50 23 363 428
Fragments arranged by size:
428 363 50 23
SfoI GGC'GCC
Cuts at: 0 246 369 864
Size: 246 123 495
Fragments arranged by size:
495 246 123
SmlI C'TyrA_G
Cuts at: 0 280 864
Size: 280 584
StyI C'CwwG_G
Cuts at: 0 195 864
Size: 195 669
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 697 729 864
Size: 433 32 232 32 135
Fragments arranged by size:
433 232 135 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 811 864
Size: 811 53
XhoI C'TCGA_G
Cuts at: 0 280 864
Size: 280 584
Enzymes that do cut:
AccI AclI AcuI AlwNI ApoI AseI AvaI BanI
BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsmI Bsp1286I
BspEI BsrDI BstEII BtgZI BtsI EaeI EarI EciI
Eco57MI EcoNI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NspI PflMI PspXI PstI PvuII
SalI SapI SfcI SfoI SmlI StyI TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SacII SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI
XmaI XmnI ZraI