Restriction map for
pFN21AE1114
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 336 CGCG CG^CG
AciI 3 GCGG CCGC(-3/-1)
AcyI 406 GGCGTC G[GA]^CG[CT]C
AlwNI 262 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 278 GGATCC G^GATCC
BanI 5 GGTGCC G^G[CT][GA]CC
BcnI 379 CCGGG CC^[GC]GG
BfaI 47 CTAG C^TAG
BsaHI 406 GGCGTC G[GA]^CG[CT]C
BseRI 66 GAGGAG GAGGAG(10/8)
BsmAI 29 GTCTC GTCTC(1/5)
BspMI 457 GCAGGT ACCTGC(4/8)
BstAPI 356 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 336 CGCG CG^CG
BstXI 9 CCACGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 335 GCGC GCG^C
DrdI 20 GACCTTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 15 CCATGG C^C[GA][CT]GG
EaeI 38 CGGCCA [CT]^GGCC[GA]
EarI 463 GAAGAG CTCTTC(1/4)
EcoT14I 15 CCATGG C^C[AT][AT]GG
HgaI 407 GCGTC GACGC(5/10)
HhaI 335 GCGC GCG^C
Hin1I 406 GGCGTC G[GA]^CG[CT]C
HinP1I 335 GCGC G^CGC
Ksp632I 463 GAAGAG CTCTTC(1/4)
MaeI 47 CTAG C^TAG
MaeIII 34 GTCAC ^GT[ACGT]AC
MslI 10 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 336 CGCG CG^CG
MwoI 356 GCAAGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 379 CCGGG CC^[GC]GG
NcoI 15 CCATGG C^CATGG
SapI 463 GAAGAGC GCTCTTC(1/4)
SfaNI 365 GCATC GCATC(5/9)
StyI 15 CCATGG C^C[AT][AT]GG
TfiI 146 GATTC G^A[AT]TC
Tsp45I 34 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 19 GGACC G^G[AT]CC
166 GGACC
BciVI 314 GGATAC GTATCC(6/5)
108 GTATCC
BstYI 278 GGATCC [GA]^GATC[CT]
325 AGATCC
DdeI 80 CTCAG C^T[ACGT]AG
322 CTGAG
HaeIII 39 GGCC GG^CC
62 GGCC
HapII 379 CCGG C^CGG
404 CCGG
HpaII 379 CCGG C^CGG
404 CCGG
MflI 278 GGATCC [GA]^GATC[CT]
325 AGATCC
MspA1I 265 CAGCTG C[AC]G^C[GT]G
319 CAGCTG
MspI 379 CCGG C^CGG
404 CCGG
PleI 153 GACTC GAGTC(4/5)
114 GAGTC
PvuII 265 CAGCTG CAG^CTG
319 CAGCTG
TspRI 331 CAGTG CA[GC]TG[ACGT][ACGT]^
439 CACTG
XhoII 278 GGATCC [GA]^GATC[CT]
325 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cfr13I DpnII
EcoRII Fnu4HI HinfI HphI ItaI
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TseI