################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AvaI 82 CCCGAG C^[CT]CG[GA]G BamHI 150 GGATCC G^GATCC BfaI 32 CTAG C^TAG BglI 72 GCCTATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 46 TACGTA [CT]AC^GT[GA] BseRI 304 GAGGAG GAGGAG(10/8) BsoBI 82 CCCGAG C^[CT]CG[GA]G BspMI 193 GCAGGT ACCTGC(4/8) BsrI 247 ACTGG ACTGG(1/-1) BssKI 225 CCAGG ^CC[ACGT]GG BstEII 235 GGTCACC G^GT[ACGT]ACC BstF5I 177 GGATG GGATG(2/0) BstNI 225 CCAGG CC^[AT]GG BstPI 235 GGTCACC G^GT[ACGT]ACC BstYI 150 GGATCC [GA]^GATC[CT] DdeI 164 CTCAG C^T[ACGT]AG EcoO65I 235 GGTCACC G^GT[ACGT]ACC EcoRII 225 CCAGG ^CC[AT]GG FauI 291 CCCGC CCCGC(4/6) FokI 177 GGATG GGATG(9/13) HgaI 41 GACGC GACGC(5/10) MaeI 32 CTAG C^TAG MaeIII 236 GTCAC ^GT[ACGT]AC MflI 150 GGATCC [GA]^GATC[CT] MspA1I 158 CAGCTG C[AC]G^C[GT]G MvaI 225 CCAGG CC^[AT]GG PflMI 241 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 158 CAGCTG CAG^CTG ScrFI 225 CCAGG CC^[ACGT]GG SfaNI 187 GCATC GCATC(5/9) SfiI 71 GGCCTATGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 46 TACGTA TAC^GTA TaqI 19 TCGA T^CGA Tsp45I 236 GTCAC ^GT[GC]AC TthHB8I 19 TCGA T^CGA Van91I 241 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 150 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 292 CCGC CCGC(-3/-1) 78 GCGG AluI 159 AGCT AG^CT 216 AGCT AlwI 151 GATCC GGATC(4/5) 150 GGATC AvaII 268 GGTCC G^G[AT]CC 288 GGACC BbvI 157 GCAGC GCAGC(8/12) 160 GCTGC DpnII 17 GATC ^GATC 151 GATC EcoNI 118 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 319 CCTGAAGAAGG MaeII 47 ACGT A^CGT 144 ACGT MboI 17 GATC ^GATC 151 GATC MwoI 72 GCCTATGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 78 GCGGCCCGAGC NdeII 17 GATC ^GATC 151 GATC PstI 155 CTGCAG CTGCA^G 191 CTGCAG Sau3AI 17 GATC ^GATC 151 GATC SfcI 155 CTGCAG C^T[GA][CT]AG 191 CTGCAG TseI 157 GCAGC G^C[AT]GC 160 GCTGC TspRI 93 CAGTG CA[GC]TG[ACGT][ACGT]^ 343 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BsiYI BslI Cfr13I Fnu4HI HaeIII HphI ItaI MboII MnlI NlaIV Sau96I