Restriction map for
pFN21AE1110
(Circular) (Six-base) MAPSORT of: pFN21AE1110.seq Check: 3926 from: 1 to: 5053
pFN21AE1110 5053 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2863 278
Size: 53 83 186 936 1327 2468
Fragments arranged by size:
2468 1327 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2396 1937
Size: 459 4594
Acc65I G'GTAC_C
Cuts at: 2374 2374
Size: 5053
AclI AA'CG_TT
Cuts at: 1563 3180 3553 1563
Size: 1617 373 3063
Fragments arranged by size:
3063 1617 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 2238 2346 3116 4500 4768 1632
Size: 513 93 108 770 1384 268 1917
Fragments arranged by size:
1917 1384 770 513 268 108 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4856
AflIII A'CryG_T
Cuts at: 1203 3952 4929 1203
Size: 2749 977 1327
Fragments arranged by size:
2749 1327 977
AgeI A'CCGG_T
Cuts at: 3884 3884
Size: 5053
AhdI GACnn_n'nnGTC
Cuts at: 3782 3782
Size: 5053
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5053
AlwNI CAG_nnn'CTG
Cuts at: 2796 4368 2796
Size: 1572 3481
ApaLI G'TGCA_C
Cuts at: 3110 4266 3110
Size: 1156 3897
ApoI r'AATT_y
Cuts at: 1493 2362 2588 2740 4849 1493
Size: 869 226 152 2109 1697
Fragments arranged by size:
2109 1697 869 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3607 160
Size: 872 2575 1606
Fragments arranged by size:
2575 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5053
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2378 1870
Size: 73 144 291 4545
Fragments arranged by size:
4545 291 144 73
BamHI G'GATC_C
Cuts at: 2155 2383 2750 2155
Size: 228 367 4458
Fragments arranged by size:
4458 367 228
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 3829 618
Size: 325 217 627 587 1455 1842
Fragments arranged by size:
1842 1455 627 587 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 1391
Size: 981 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3244 3278 1903
Size: 34 1307 34 3678
Fragments arranged by size:
3678 1307 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2945 4155 2945
Size: 1210 3843
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2083 3664 136
Size: 107 122 71 631 605 411 1581 1525
Fragments arranged by size:
1581 1525 631 605 411 122 107 71
BglII A'GATC_T
Cuts at: 5048 5048
Size: 5053
BlpI GC'TnA_GC
Cuts at: 2470 2470
Size: 5053
Bme1580I G_kGCm'C
Cuts at: 1790 3114 4270 1790
Size: 1324 1156 2573
Fragments arranged by size:
2573 1324 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2262 2784 3742
Size: 416 53 83 399 24 845 522 958
Cuts at: 3742 442
Size: 1753
Fragments arranged by size:
1753 958 845 522 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3713 1239
Size: 2474 2579
Bpu10I CC'TnA_GC
Cuts at: 2169 2169
Size: 5053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3178 4043 4341 3178
Size: 865 298 3890
Fragments arranged by size:
3890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3716 882
Size: 617 2217 2219
Fragments arranged by size:
2219 2217 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 2844 493
Size: 711 849 791 2702
Fragments arranged by size:
2702 849 791 711
BsaBI GATnn'nnATC
Cuts at: 5047 5047
Size: 5053
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2860 3242 3877 275
Size: 53 83 186 936 1327 382 635 1451
Fragments arranged by size:
1451 1327 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3486 3884 4158 4305 4716 3486
Size: 398 274 147 411 3823
Fragments arranged by size:
3823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4828
Fragments arranged by size:
4828 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 1186
Size: 1138 3915
BseYI C'CCAG_C
Cuts at: 4256 4752 4256
Size: 496 4557
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4480
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2503 3264 4292 1540
Size: 275 186 502 761 1028 2301
Fragments arranged by size:
2301 1028 761 502 275 186
BsiHKAI G_wGCw'C
Cuts at: 3114 3199 4270 3114
Size: 85 1071 3897
Fragments arranged by size:
3897 1071 85
BsmI GAATG_Cn'
Cuts at: 2564 2657 2564
Size: 93 4960
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2372 3114 3199 4270 1391
Size: 399 582 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 399 85
BspEI T'CCGG_A
Cuts at: 4716 4716
Size: 5053
BspHI T'CATG_A
Cuts at: 2942 4672 2942
Size: 1730 3323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 2408 844
Size: 1345 219 3489
Fragments arranged by size:
3489 1345 219
BsrBI CCG'CTC
Cuts at: 1394 1823 2940 1394
Size: 429 1117 3507
Fragments arranged by size:
3507 1117 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3548 3722 65
Size: 1183 2300 174 1396
Fragments arranged by size:
2300 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3697 3884 1700
Size: 110 1887 187 2869
Fragments arranged by size:
2869 1887 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5053
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5053
BssSI C'ACGA_G
Cuts at: 3113 4125 3113
Size: 1012 4041
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5053
BstBI TT'CG_AA
Cuts at: 3857 3857
Size: 5053
BstEII G'GTnAC_C
Cuts at: 1184 2240 1184
Size: 1056 3997
BstYI r'GATC_y
Cuts at: 1972 2155 2383 2750 3135 3152 4604 4690 4702
Size: 183 228 367 385 17 1452 86 12
Cuts at: 4702 5048 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 228 183 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4501
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4314
Fragments arranged by size:
4314 683 56
BtsI GCAGTG_nn'
Cuts at: 2569 3363 3383 2569
Size: 794 20 4239
Fragments arranged by size:
4239 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5053
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5018
DraI TTT'AAA
Cuts at: 2357 2357
Size: 5053
DraIII CAC_nnn'GTG
Cuts at: 2351 2351
Size: 5053
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4060 809
Size: 507 2744 1802
Fragments arranged by size:
2744 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 3389 5018
Size: 54 1425 50 275 18 670 889 1629
Cuts at: 5018 8
Size: 43
Fragments arranged by size:
1629 1425 889 670 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2500 1537
Size: 275 688 4090
Fragments arranged by size:
4090 688 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2983 1353
Size: 1630 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3640 4014 4160 387
Size: 1064 138 2051 374 146 1280
Fragments arranged by size:
2051 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2238 2346 3116 3713 4500 4768
Size: 393 513 93 108 770 597 787 268
Cuts at: 4768 1239
Size: 1524
Fragments arranged by size:
1524 787 770 597 513 393 268 108
93
EcoNI CCTnn'n_nnAGG
Cuts at: 2127 2328 2127
Size: 201 4852
EcoRI G'AATT_C
Cuts at: 2362 2740 2362
Size: 378 4675
FspI TGC'GCA
Cuts at: 3559 3559
Size: 5053
HaeII r_GCGC'y
Cuts at: 1678 4200 1678
Size: 2522 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 3694 3726 3768
Size: 32 135 32 605 32 1485 32 42
Cuts at: 3768 3800 1373
Size: 32 2626
Fragments arranged by size:
2626 1485 605 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2397 2643 669
Size: 988 281 459 246 3079
Fragments arranged by size:
3079 988 459 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5053
HpaI GTT'AAC
Cuts at: 2643 2643
Size: 5053
KpnI G_GTAC'C
Cuts at: 2378 2378
Size: 5053
MfeI C'AATT_G
Cuts at: 2652 2652
Size: 5053
MluI A'CGCG_T
Cuts at: 4929 4929
Size: 5053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4167 4351 1305
Size: 2862 184 2007
Fragments arranged by size:
2862 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3231
Fragments arranged by size:
3231 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 3370 3529 518
Size: 648 1845 359 159 2042
Fragments arranged by size:
2042 1845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2165 2469 3146 4294 4539 1606
Size: 559 304 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 559 304 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4501
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5053
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5053
NotI GC'GGCC_GC
Cuts at: 2500 2500
Size: 5053
NspI r_CATG'y
Cuts at: 1132 1229 2411 3956 1132
Size: 97 1182 1545 2229
Fragments arranged by size:
2229 1545 1182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5053
PciI A'CATG_T
Cuts at: 3952 3952
Size: 5053
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2252 1108
Size: 902 242 3909
Fragments arranged by size:
3909 902 242
PmeI GTTT'AAAC
Cuts at: 2357 2357
Size: 5053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 4635 4667 3122
Size: 32 1481 32 3508
Fragments arranged by size:
3508 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5053
PsiI TTA'TAA
Cuts at: 2623 2623
Size: 5053
PstI C_TGCA'G
Cuts at: 830 2164 2200 2405 830
Size: 1334 36 205 3478
Fragments arranged by size:
3478 1334 205 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5053
PvuII CAG'CTG
Cuts at: 2165 2165
Size: 5053
SalI G'TCGA_C
Cuts at: 1936 2395 1936
Size: 459 4594
SbfI CC_TGCA'GG
Cuts at: 2405 2405
Size: 5053
ScaI AGT'ACT
Cuts at: 3301 5013 3301
Size: 1712 3341
SfcI C'TryA_G
Cuts at: 826 1045 2160 2196 2401 3536 4217 4408 826
Size: 219 1115 36 205 1135 681 191 1471
Fragments arranged by size:
1471 1135 1115 681 219 205 191 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 2083 2083
Size: 5053
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5053
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5053
SmaI CCC'GGG
Cuts at: 1872 2380 1872
Size: 508 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3157 4058 4320 820
Size: 197 926 1214 901 262 1553
Fragments arranged by size:
1553 1214 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2053 2844 493
Size: 1560 791 2702
Fragments arranged by size:
2702 1560 791
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5053
SphI G_CATG'C
Cuts at: 1132 2411 1132
Size: 1279 3774
SspI AAT'ATT
Cuts at: 5 52 2977 5
Size: 47 2925 2081
Fragments arranged by size:
2925 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5053
StyI C'CwwG_G
Cuts at: 513 1065 2492 513
Size: 552 1427 3074
Fragments arranged by size:
3074 1427 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 2259 3080 3097 3250 3435 3880
Size: 1255 664 15 821 17 153 185 445
Cuts at: 3880 325
Size: 1498
Fragments arranged by size:
1498 1255 821 664 445 185 153 17
15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3299 5011 96
Size: 274 80 33 51 1443 1322 1712 138
Fragments arranged by size:
1712 1443 1322 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5013 5045 5013
Size: 32 5021
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5053
XbaI T'CTAG_A
Cuts at: 2389 2389
Size: 5053
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5053
XmaI C'CCGG_G
Cuts at: 1870 2378 1870
Size: 508 4545
XmnI GAAnn'nnTTC
Cuts at: 3182 3182
Size: 5053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2861 276
Size: 53 83 186 936 1327 2468
Fragments arranged by size:
2468 1327 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI XcmI