################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI Eam1105I Eco81I EcoO65I EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RcaI SacI SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 1087 GTCTAC GT^[AC][GT]AC AcsI 427 GAATTC [GA]^AATT[CT] Aor51HI 1549 AGCGCT AGC^GCT ApaI 236 GGGCCC GGGCC^C ApoI 427 GAATTC [GA]^AATT[CT] BglI 1837 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1233 AGATCT A^GATCT BlpI 1552 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1552 GCTGAGC GC^T[ACGT]AGC BssHII 1701 GCGCGC G^CGCGC BstBI 703 TTCGAA TT^CGAA CelII 1552 GCTGAGC GC^T[ACGT]AGC CpoI 1488 CGGACCG CG^G[AT]CCG DraI 124 TTTAAA TTT^AAA EagI 689 CGGCCG C^GGCCG EarI 1686 GAAGAG CTCTTC(1/4) EclXI 689 CGGCCG C^GGCCG Eco47III 1549 AGCGCT AGC^GCT Eco52I 689 CGGCCG C^GGCCG EcoNI 1674 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 427 GAATTC G^AATTC FseI 1647 GGCCGGCC GGCCGG^CC Ksp632I 1686 GAAGAG CTCTTC(1/4) KspI 692 CCGCGG CCGC^GG NaeI 1648 GCCGGC GCC^GGC NgoMIV 1648 GCCGGC G^CCGGC NruI 374 TCGCGA TCG^CGA NspV 703 TTCGAA TT^CGAA RsrII 1488 CGGACCG CG^G[AT]CCG SacII 692 CCGCGG CCGC^GG SexAI 1799 ACCAGGT A^CC[AT]GGT SfcI 321 CTACAG C^T[GA][CT]AG SfuI 703 TTCGAA TT^CGAA SphI 548 GCATGC GCATG^C Tsp509I 428 AATT ^AATT XcmI 1031 CCATGGGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 316 GACGCC G[GA]^CG[CT]C 985 GACGCC Alw44I 1388 GTGCAC G^TGCAC 1813 GTGCAC ApaLI 1388 GTGCAC G^TGCAC 1813 GTGCAC Asp700I 603 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 921 GAAGGTGTTC AspHI 1388 GTGCAC G[AT]GC[AT]^C 1813 GTGCAC AspI 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1597 GACAAAGTC AvaI 830 CTCGGG C^[CT]CG[GA]G 1397 CCCGAG BamHI 530 GGATCC G^GATCC 1574 GGATCC BbsI 706 GAAGAC GAAGAC(2/6) 1047 GAAGAC BbvCI 81 CCTCAGC CCTCAGC(-5/-2) 1275 GCTGAGG BpuAI 706 GAAGAC GAAGAC(2/6) 1047 GAAGAC BsaHI 316 GACGCC G[GA]^CG[CT]C 985 GACGCC BsiHKAI 1388 GTGCAC G[AT]GC[AT]^C 1813 GTGCAC BsmBI 1267 GAGACG CGTCTC(1/5) 1666 GAGACG BsoBI 830 CTCGGG C^[CT]CG[GA]G 1397 CCCGAG BsrBI 257 GAGCGG CCGCTC(-3/-3) 1750 GAGCGG BsrI 1392 ACTGG ACTGG(1/-1) 1586 ACTGG BstAPI 1494 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1806 GCAGGTGGTGC BstXI 974 CCAGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1847 CCACCAAAGTGG DraIII 355 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1798 CACCAGGTG DrdI 412 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1594 GACGACAAAGTC HaeII 55 AGCGCC [GA]GCGC^[CT] 1549 AGCGCT Hin1I 316 GACGCC G[GA]^CG[CT]C 985 GACGCC MseI 125 TTAA T^TAA 937 TTAA NspI 548 GCATGC [GA]CATG^[CT] 1724 ACATGC PflFI 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1597 GACAAAGTC PflMI 402 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 534 CCAGGCCCTGG PpuMI 653 AGGACCT [GA]G^G[AT]CC[CT] 1535 GGGACCC PvuI 266 CGATCG CGAT^CG 377 CGATCG PvuII 406 CAGCTG CAG^CTG 619 CAGCTG TfiI 288 GATTC G^A[AT]TC 593 GATTC Tru9I 125 TTAA T^TAA 937 TTAA Tth111I 244 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1597 GACAAAGTC Van91I 402 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 534 CCAGGCCCTGG XmnI 603 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 921 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII