(Circular) (Six-base) MAPSORT of: pFN21AE2031.seq Check: 4162 from: 1 to: 6568 pFN21AE2031 6568 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3424 3515 3802 3424 Size: 91 287 6190 Fragments arranged by size: 6190 287 91 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3093 3911 1937 Size: 1156 818 4594 Fragments arranged by size: 4594 1156 818 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2945 3765 4631 6015 6283 1632 Size: 1313 820 866 1384 268 1917 Fragments arranged by size: 1917 1384 1313 866 820 268 AfeI AGC'GCT Cuts at: 3556 3556 Size: 6568 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 2273 3857 1166 Size: 1107 1584 3877 Fragments arranged by size: 3877 1584 1107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6568 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3227 3259 3227 Size: 32 6536 AlwNI CAG_nnn'CTG Cuts at: 2545 2819 3724 4311 5883 2545 Size: 274 905 587 1572 3230 Fragments arranged by size: 3230 1572 905 587 274 ApaI G_GGCC'C Cuts at: 2245 2245 Size: 6568 ApaLI G'TGCA_C Cuts at: 3393 3818 4625 5781 3393 Size: 425 807 1156 4180 Fragments arranged by size: 4180 1156 807 425 ApoI r'AATT_y Cuts at: 1493 2432 3877 4103 4255 6364 1493 Size: 939 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 939 226 152 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2835 3402 3893 1870 Size: 73 892 567 491 4545 Fragments arranged by size: 4545 892 567 491 73 BamHI G'GATC_C Cuts at: 2535 3579 3898 4265 2535 Size: 1044 319 367 4838 Fragments arranged by size: 4838 1044 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3889 5344 618 Size: 325 217 627 2102 1455 1842 Fragments arranged by size: 2102 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2245 2511 2748 3045 3269 3887 1391 Size: 854 266 237 297 224 618 4072 Fragments arranged by size: 4072 854 618 297 266 237 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2718 3059 928 Size: 971 819 341 4437 Fragments arranged by size: 4437 971 819 341 BbvCI CC'TCA_GC Cuts at: 2087 3281 2087 Size: 1194 5374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4460 5670 4460 Size: 1210 5358 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3848 5179 136 Size: 107 122 71 631 605 2176 1331 1525 Fragments arranged by size: 2176 1525 1331 631 605 122 107 71 BglII A'GATC_T Cuts at: 3238 6563 3238 Size: 3325 3243 BlpI GC'TnA_GC Cuts at: 3558 3985 3558 Size: 427 6141 Bme1580I G_kGCm'C Cuts at: 1790 2018 2245 3397 3822 4629 5785 1790 Size: 228 227 1152 425 807 1156 2573 Fragments arranged by size: 2573 1156 1152 807 425 228 227 BmgBI CAC'GTC Cuts at: 1318 2251 2725 3376 1318 Size: 933 474 651 4510 Fragments arranged by size: 4510 933 651 474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4299 5257 442 Size: 416 53 83 399 24 2882 958 1753 Fragments arranged by size: 2882 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2318 2380 2891 3068 3197 3806 5228 Size: 986 93 62 511 177 129 609 1422 Cuts at: 5228 1239 Size: 2579 Fragments arranged by size: 2579 1422 986 609 511 177 129 93 62 Bpu10I CC'TnA_GC Cuts at: 2087 3281 2087 Size: 1194 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4693 5558 5856 4693 Size: 865 298 5405 Fragments arranged by size: 5405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2105 2177 2431 2834 5231 882 Size: 617 606 72 254 403 2397 2219 Fragments arranged by size: 2397 2219 617 606 403 254 72 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 2991 4375 4757 Size: 53 83 186 936 789 669 1384 382 Cuts at: 4757 5392 275 Size: 635 1451 Fragments arranged by size: 1451 1384 936 789 669 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5001 5399 5673 5820 6231 5001 Size: 398 274 147 411 5338 Fragments arranged by size: 5338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2135 2165 2833 2863 1178 Size: 30 165 30 732 30 668 30 4883 Fragments arranged by size: 4883 732 668 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3352 3701 1186 Size: 1303 863 349 4053 Fragments arranged by size: 4053 1303 863 349 BseYI C'CCAG_C Cuts at: 3551 5771 6267 3551 Size: 2220 496 3852 Fragments arranged by size: 3852 2220 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2021 3107 3370 3527 3758 3776 3830 Size: 573 285 1086 263 157 231 18 54 Cuts at: 3830 1163 Size: 3901 Fragments arranged by size: 3901 1086 573 285 263 231 157 54 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2274 2385 2697 4018 4779 5807 Size: 275 186 273 111 312 1321 761 1028 Cuts at: 5807 1540 Size: 2301 Fragments arranged by size: 2301 1321 1028 761 312 275 273 186 111 BsiHKAI G_wGCw'C Cuts at: 3397 3822 4629 4714 5785 3397 Size: 425 807 85 1071 4180 Fragments arranged by size: 4180 1071 807 425 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 BsmBI CGTCTCn'nnnn_ Cuts at: 3266 3665 3266 Size: 399 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2245 2511 2748 3045 3269 3397 Size: 399 228 227 266 237 297 224 128 Cuts at: 3397 3822 3887 4629 4714 5785 1391 Size: 425 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 425 399 297 266 237 228 227 224 128 85 65 BspEI T'CCGG_A Cuts at: 6231 6231 Size: 6568 BspHI T'CATG_A Cuts at: 4457 6187 4457 Size: 1730 4838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2670 3424 3515 3802 3923 844 Size: 1400 426 754 91 287 121 3489 Fragments arranged by size: 3489 1400 754 426 287 121 91 BsrBI CCG'CTC Cuts at: 1394 1823 2264 3757 4455 1394 Size: 429 441 1493 698 3507 Fragments arranged by size: 3507 1493 698 441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5063 5237 65 Size: 1183 3815 174 1396 Fragments arranged by size: 3815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 2305 3653 5212 5399 1700 Size: 110 407 88 1348 1559 187 2869 Fragments arranged by size: 2869 1559 1348 407 187 110 88 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6568 BssHII G'CGCG_C Cuts at: 1925 3706 1925 Size: 1781 4787 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 3505 3817 794 Size: 2711 312 3545 Fragments arranged by size: 3545 2711 312 BstBI TT'CG_AA Cuts at: 2709 5372 2709 Size: 2663 3905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstXI CCAn_nnnn'nTGG Cuts at: 2986 3859 2986 Size: 873 5695 BstYI r'GATC_y Cuts at: 1972 2082 2535 3238 3510 3579 3898 4265 4650 Size: 110 453 703 272 69 319 367 385 Cuts at: 4650 4667 6119 6205 6217 6563 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 703 453 385 367 346 319 272 110 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2178 2511 2697 2808 3036 3336 Size: 552 939 174 333 186 111 228 300 Cuts at: 3336 513 Size: 3745 Fragments arranged by size: 3745 939 552 333 300 228 186 174 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2787 487 Size: 683 56 1561 4268 Fragments arranged by size: 4268 1561 683 56 BtsI GCAGTG_nn' Cuts at: 2389 3510 4084 4878 4898 2389 Size: 1121 574 794 20 4059 Fragments arranged by size: 4059 1121 794 574 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 2131 3872 2131 Size: 1741 4827 DraIII CAC_nnn'GTG Cuts at: 2365 3808 2365 Size: 1443 5125 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2423 3605 5575 809 Size: 507 1107 1182 1970 1802 Fragments arranged by size: 1970 1802 1182 1107 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2211 2694 Size: 54 1425 50 275 18 225 156 483 Cuts at: 2694 3333 3471 3593 3849 4015 4904 6533 8 Size: 639 138 122 256 166 889 1629 43 Fragments arranged by size: 1629 1425 889 639 483 275 256 225 166 156 138 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2694 4015 1537 Size: 275 882 1321 4090 Fragments arranged by size: 4090 1321 882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3686 4498 1353 Size: 2333 812 3423 Fragments arranged by size: 3423 2333 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2683 3248 3355 5155 5529 5675 Size: 1064 138 1094 565 107 1800 374 146 Cuts at: 5675 387 Size: 1280 Fragments arranged by size: 1800 1280 1094 1064 565 374 146 138 107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2318 2380 2891 2945 3068 3197 Size: 393 593 93 62 511 54 123 129 Cuts at: 3197 3765 3806 4631 5228 6015 6283 1239 Size: 568 41 825 597 787 268 1524 Fragments arranged by size: 1524 825 787 597 593 568 511 393 268 129 123 93 62 54 41 EcoNI CCTnn'n_nnAGG Cuts at: 3683 3683 Size: 6568 EcoO109I rG'GnC_Cy Cuts at: 2200 2242 2542 2659 2902 3541 2200 Size: 42 300 117 243 639 5227 Fragments arranged by size: 5227 639 300 243 117 42 EcoRI G'AATT_C Cuts at: 2432 3877 4255 2432 Size: 1445 378 4745 Fragments arranged by size: 4745 1445 378 FseI GG_CCGG'CC Cuts at: 3657 3657 Size: 6568 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 2064 3558 5715 1678 Size: 386 1494 2157 2531 Fragments arranged by size: 2531 2157 1494 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2833 2865 5209 Size: 32 135 32 563 32 666 32 2344 Cuts at: 5209 5241 5283 5315 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2344 666 563 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3912 4158 669 Size: 988 281 1974 246 3079 Fragments arranged by size: 3079 1974 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3508 5682 5866 1305 Size: 2203 2174 184 2007 Fragments arranged by size: 2203 2174 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2213 3335 3595 3851 Size: 54 1425 343 225 156 1122 260 256 Cuts at: 3851 10 Size: 2727 Fragments arranged by size: 2727 1425 1122 343 260 256 225 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 2273 2558 3144 3734 3857 4526 4885 Size: 648 1107 285 586 590 123 669 359 Cuts at: 4885 5044 518 Size: 159 2042 Fragments arranged by size: 2042 1107 669 648 590 586 359 285 159 123 MspA1I CmG'CkG Cuts at: 1606 2413 2502 2605 2626 2699 3984 4661 5809 Size: 807 89 103 21 73 1285 677 1148 Cuts at: 5809 6054 1606 Size: 245 2120 Fragments arranged by size: 2120 1285 1148 807 677 245 103 89 73 21 NaeI GCC'GGC Cuts at: 3655 3655 Size: 6568 NcoI C'CATG_G Cuts at: 513 1065 2004 2178 2511 3036 513 Size: 552 939 174 333 525 4045 Fragments arranged by size: 4045 939 552 525 333 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NgoMIV G'CCGG_C Cuts at: 3653 3653 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NruI TCG'CGA Cuts at: 2381 2381 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 2557 3733 3926 5471 1132 Size: 97 1328 1176 193 1545 2229 Fragments arranged by size: 2229 1545 1328 1176 193 97 PasI CC'CwG_GG Cuts at: 1348 2545 1348 Size: 1197 5371 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2413 2545 1108 Size: 1305 132 5131 Fragments arranged by size: 5131 1305 132 PfoI T'CCnGG_A Cuts at: 2868 3045 3246 3678 3834 2868 Size: 177 201 432 156 5602 Fragments arranged by size: 5602 432 201 177 156 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2659 3541 2659 Size: 882 5686 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PspOMI G'GGCC_C Cuts at: 2241 2241 Size: 6568 PstI C_TGCA'G Cuts at: 830 3920 830 Size: 3090 3478 PvuI CG_AT'CG Cuts at: 2001 2274 2385 2001 Size: 273 111 6184 Fragments arranged by size: 6184 273 111 PvuII CAG'CTG Cuts at: 2413 2626 2413 Size: 213 6355 RsrII CG'GwC_CG Cuts at: 3494 3494 Size: 6568 SacII CC_GC'GG Cuts at: 2700 2700 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SanDI GG'GwC_CC Cuts at: 3541 3541 Size: 6568 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SexAI A'CCwGG_T Cuts at: 3804 3804 Size: 6568 SfcI C'TryA_G Cuts at: 826 1045 2326 3916 5051 5732 5923 826 Size: 219 1281 1590 1135 681 191 1471 Fragments arranged by size: 1590 1471 1281 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4672 5573 5835 820 Size: 197 926 2729 901 262 1553 Fragments arranged by size: 2729 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 2557 3926 1132 Size: 1425 1369 3774 Fragments arranged by size: 3774 1425 1369 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 2511 2898 3036 4007 513 Size: 552 939 174 333 387 138 971 3074 Fragments arranged by size: 3074 971 939 552 387 333 174 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4595 4612 4765 4950 5395 325 Size: 1255 3015 17 153 185 445 1498 Fragments arranged by size: 3015 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4814 6526 96 Size: 274 80 33 51 1443 2837 1712 138 Fragments arranged by size: 2837 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6528 6560 6528 Size: 32 6536 Tth111I GACn'n_nGTC Cuts at: 1316 2252 3605 1316 Size: 936 1353 4279 Fragments arranged by size: 4279 1353 936 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3043 3043 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 2612 2930 4697 2612 Size: 318 1767 4483 Fragments arranged by size: 4483 1767 318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI SacI SapI SfiI SfoI SrfI SwaI