(Linear) (Six-base) MAPSORT of: KIEE2031.seq Check: 7905 from: 1 to: 1863 KIEE2031.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1419 1510 1797 1863 Size: 1419 91 287 66 Fragments arranged by size: 1419 287 91 66 AccI GT'mk_AC Cuts at: 0 1088 1863 Size: 1088 775 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 940 1760 1863 Size: 940 820 103 Fragments arranged by size: 940 820 103 AfeI AGC'GCT Cuts at: 0 1551 1863 Size: 1551 312 AleI CACnn'nnGTG Cuts at: 0 268 1852 1863 Size: 268 1584 11 Fragments arranged by size: 1584 268 11 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1222 1254 1863 Size: 1222 32 609 Fragments arranged by size: 1222 609 32 AlwNI CAG_nnn'CTG Cuts at: 0 540 814 1719 1863 Size: 540 274 905 144 Fragments arranged by size: 905 540 274 144 ApaI G_GGCC'C Cuts at: 0 240 1863 Size: 240 1623 ApaLI G'TGCA_C Cuts at: 0 1388 1813 1863 Size: 1388 425 50 Fragments arranged by size: 1388 425 50 ApoI r'AATT_y Cuts at: 0 427 1863 Size: 427 1436 AvaI C'yCGr_G Cuts at: 0 830 1397 1863 Size: 830 567 466 Fragments arranged by size: 830 567 466 BamHI G'GATC_C Cuts at: 0 530 1574 1863 Size: 530 1044 289 Fragments arranged by size: 1044 530 289 BanII G_rGCy'C Cuts at: 0 240 506 743 1040 1264 1863 Size: 240 266 237 297 224 599 Fragments arranged by size: 599 297 266 240 237 224 BbsI GAAGACnn'nnnn_ Cuts at: 0 713 1054 1863 Size: 713 341 809 Fragments arranged by size: 809 713 341 BbvCI CC'TCA_GC Cuts at: 0 82 1276 1863 Size: 82 1194 587 Fragments arranged by size: 1194 587 82 BglI GCCn_nnn'nGGC Cuts at: 0 1843 1863 Size: 1843 20 BglII A'GATC_T Cuts at: 0 1233 1863 Size: 1233 630 BlpI GC'TnA_GC Cuts at: 0 1553 1863 Size: 1553 310 Bme1580I G_kGCm'C Cuts at: 0 13 240 1392 1817 1863 Size: 13 227 1152 425 46 Fragments arranged by size: 1152 425 227 46 13 BmgBI CAC'GTC Cuts at: 0 246 720 1371 1863 Size: 246 474 651 492 Fragments arranged by size: 651 492 474 246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 313 375 886 1063 1192 1801 1863 Size: 220 93 62 511 177 129 609 62 Fragments arranged by size: 609 511 220 177 129 93 62 62 Bpu10I CC'TnA_GC Cuts at: 0 82 1276 1863 Size: 82 1194 587 Fragments arranged by size: 1194 587 82 BsaI GGTCTCn'nnnn_ Cuts at: 0 100 172 426 829 1863 Size: 100 72 254 403 1034 Fragments arranged by size: 1034 403 254 100 72 BsaHI Gr'CG_yC Cuts at: 0 317 986 1863 Size: 317 669 877 Fragments arranged by size: 877 669 317 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 130 160 828 858 1863 Size: 130 30 668 30 1005 Fragments arranged by size: 1005 668 130 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 484 1347 1696 1863 Size: 484 863 349 167 Fragments arranged by size: 863 484 349 167 BseYI C'CCAG_C Cuts at: 0 1546 1863 Size: 1546 317 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 16 1102 1365 1522 1753 1771 1825 1863 Size: 16 1086 263 157 231 18 54 38 Fragments arranged by size: 1086 263 231 157 54 38 18 16 BsiEI CG_ry'CG Cuts at: 0 269 380 692 1863 Size: 269 111 312 1171 Fragments arranged by size: 1171 312 269 111 BsiHKAI G_wGCw'C Cuts at: 0 1392 1817 1863 Size: 1392 425 46 Fragments arranged by size: 1392 425 46 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1261 1660 1863 Size: 1261 399 203 Fragments arranged by size: 1261 399 203 Bsp1286I G_dGCh'C Cuts at: 0 13 240 506 743 1040 1264 1392 1817 Size: 13 227 266 237 297 224 128 425 Cuts at: 1817 1863 Size: 46 Fragments arranged by size: 425 297 266 237 227 224 128 46 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 239 665 1419 1510 1797 1863 Size: 239 426 754 91 287 66 Fragments arranged by size: 754 426 287 239 91 66 BsrBI CCG'CTC Cuts at: 0 259 1752 1863 Size: 259 1493 111 Fragments arranged by size: 1493 259 111 BsrFI r'CCGG_y Cuts at: 0 212 300 1648 1863 Size: 212 88 1348 215 Fragments arranged by size: 1348 215 212 88 BssHII G'CGCG_C Cuts at: 0 1701 1863 Size: 1701 162 BstAPI GCAn_nnn'nTGC Cuts at: 0 1500 1812 1863 Size: 1500 312 51 Fragments arranged by size: 1500 312 51 BstBI TT'CG_AA Cuts at: 0 704 1863 Size: 704 1159 BstXI CCAn_nnnn'nTGG Cuts at: 0 981 1854 1863 Size: 981 873 9 Fragments arranged by size: 981 873 9 BstYI r'GATC_y Cuts at: 0 77 530 1233 1505 1574 1863 Size: 77 453 703 272 69 289 Fragments arranged by size: 703 453 289 272 77 69 BtgI C'CryG_G Cuts at: 0 173 506 692 803 1031 1331 1863 Size: 173 333 186 111 228 300 532 Fragments arranged by size: 532 333 300 228 186 173 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 782 1863 Size: 782 1081 BtsI GCAGTG_nn' Cuts at: 0 384 1505 1863 Size: 384 1121 358 Fragments arranged by size: 1121 384 358 DraI TTT'AAA Cuts at: 0 126 1863 Size: 126 1737 DraIII CAC_nnn'GTG Cuts at: 0 360 1803 1863 Size: 360 1443 60 Fragments arranged by size: 1443 360 60 DrdI GACnn_nn'nnGTC Cuts at: 0 418 1600 1863 Size: 418 1182 263 Fragments arranged by size: 1182 418 263 EaeI y'GGCC_r Cuts at: 0 50 206 689 1328 1466 1588 1844 1863 Size: 50 156 483 639 138 122 256 19 Fragments arranged by size: 639 483 256 156 138 122 50 19 EagI C'GGCC_G Cuts at: 0 689 1863 Size: 689 1174 EarI CTCTTCn'nnn_ Cuts at: 0 1681 1863 Size: 1681 182 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 678 1243 1350 1863 Size: 678 565 107 513 Fragments arranged by size: 678 565 513 107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 313 375 886 940 1063 1192 1760 Size: 220 93 62 511 54 123 129 568 Cuts at: 1760 1801 1863 Size: 41 62 Fragments arranged by size: 568 511 220 129 123 93 62 62 54 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 1678 1863 Size: 1678 185 EcoO109I rG'GnC_Cy Cuts at: 0 195 237 537 654 897 1536 1863 Size: 195 42 300 117 243 639 327 Fragments arranged by size: 639 327 300 243 195 117 42 EcoRI G'AATT_C Cuts at: 0 427 1863 Size: 427 1436 FseI GG_CCGG'CC Cuts at: 0 1652 1863 Size: 1652 211 HaeII r_GCGC'y Cuts at: 0 59 1553 1863 Size: 59 1494 310 Fragments arranged by size: 1494 310 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 828 860 1863 Size: 130 32 666 32 1003 Fragments arranged by size: 1003 666 130 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1503 1863 Size: 1503 360 MscI TGG'CCA Cuts at: 0 52 208 1330 1590 1846 1863 Size: 52 156 1122 260 256 17 Fragments arranged by size: 1122 260 256 156 52 17 MslI CAynn'nnrTG Cuts at: 0 268 553 1139 1729 1852 1863 Size: 268 285 586 590 123 11 Fragments arranged by size: 590 586 285 268 123 11 MspA1I CmG'CkG Cuts at: 0 408 497 600 621 694 1863 Size: 408 89 103 21 73 1169 Fragments arranged by size: 1169 408 103 89 73 21 NaeI GCC'GGC Cuts at: 0 1650 1863 Size: 1650 213 NcoI C'CATG_G Cuts at: 0 173 506 1031 1863 Size: 173 333 525 832 Fragments arranged by size: 832 525 333 173 NgoMIV G'CCGG_C Cuts at: 0 1648 1863 Size: 1648 215 NruI TCG'CGA Cuts at: 0 376 1863 Size: 376 1487 NspI r_CATG'y Cuts at: 0 552 1728 1863 Size: 552 1176 135 Fragments arranged by size: 1176 552 135 PasI CC'CwG_GG Cuts at: 0 540 1863 Size: 540 1323 PflMI CCAn_nnn'nTGG Cuts at: 0 408 540 1863 Size: 408 132 1323 Fragments arranged by size: 1323 408 132 PfoI T'CCnGG_A Cuts at: 0 863 1040 1241 1673 1829 1863 Size: 863 177 201 432 156 34 Fragments arranged by size: 863 432 201 177 156 34 PpuMI rG'GwC_Cy Cuts at: 0 654 1536 1863 Size: 654 882 327 Fragments arranged by size: 882 654 327 PspOMI G'GGCC_C Cuts at: 0 236 1863 Size: 236 1627 PvuI CG_AT'CG Cuts at: 0 269 380 1863 Size: 269 111 1483 Fragments arranged by size: 1483 269 111 PvuII CAG'CTG Cuts at: 0 408 621 1863 Size: 408 213 1242 Fragments arranged by size: 1242 408 213 RsrII CG'GwC_CG Cuts at: 0 1489 1863 Size: 1489 374 SacII CC_GC'GG Cuts at: 0 695 1863 Size: 695 1168 SanDI GG'GwC_CC Cuts at: 0 1536 1863 Size: 1536 327 SexAI A'CCwGG_T Cuts at: 0 1799 1863 Size: 1799 64 SfcI C'TryA_G Cuts at: 0 321 1863 Size: 321 1542 SphI G_CATG'C Cuts at: 0 552 1863 Size: 552 1311 StyI C'CwwG_G Cuts at: 0 173 506 893 1031 1863 Size: 173 333 387 138 832 Fragments arranged by size: 832 387 333 173 138 Tth111I GACn'n_nGTC Cuts at: 0 247 1600 1863 Size: 247 1353 263 Fragments arranged by size: 1353 263 247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1038 1863 Size: 1038 825 XmnI GAAnn'nnTTC Cuts at: 0 607 925 1863 Size: 607 318 938 Fragments arranged by size: 938 607 318 Enzymes that do cut: AarI AccI AcuI AfeI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NruI NspI PasI PflMI PfoI PpuMI PspOMI PvuI PvuII RsrII SacII SanDI SexAI SfcI SphI StyI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstEII BstZ17I Bsu36I ClaI CspCI EcoICRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI SacI SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XhoI XmaI ZraI