################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvrII BalI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI Hin1I HpaI KasI KpnI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tsp45I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 32 CGCG CG^CG AccIII 893 TCCGGA T^CCGGA AfaI 1165 GTAC GT^AC AlwNI 1546 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 356 AGCGCT AGC^GCT AseI 1192 ATTAAT AT^TAAT AsnI 1192 ATTAAT AT^TAAT AvaI 1252 CCCGGG C^[CT]CG[GA]G AvaII 1144 GGTCC G^G[AT]CC AviII 1484 TGCGCA TGC^GCA BamHI 711 GGATCC G^GATCC BbsI 566 GTCTTC GAAGAC(2/6) BcgI 315 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 566 GTCTTC GAAGAC(2/6) BsaBI 1258 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 893 TCCGGA [AT]^CCGG[AT] BseAI 893 TCCGGA T^CCGGA BsmI 956 GAATGC GAATGC(1/-1) BsoBI 1252 CCCGGG C^[CT]CG[GA]G BspEI 893 TCCGGA T^CCGGA BspLU11I 887 ACATGT A^CATGT BsrDI 785 CATTGC GCAATG(2/0) BstUI 32 CGCG CG^CG BstXI 1587 CCAGTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 1143 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 31 CCGCGG C^C[GA][CT]GG EaeI 1103 CGGCCA [CT]^GGCC[GA] EarI 1420 CTCTTC CTCTTC(1/4) Eco47III 356 AGCGCT AGC^GCT EcoO109I 1143 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 148 GATATC GAT^ATC EcoT14I 1711 CCAAGG C^C[AT][AT]GG FspI 1484 TGCGCA TGC^GCA HaeII 356 AGCGCT [GA]GCGC^[CT] HincII 883 GTTGAC GT[CT]^[GA]AC HindII 883 GTTGAC GT[CT]^[GA]AC HindIII 1388 AAGCTT A^AGCTT Ksp632I 1420 CTCTTC CTCTTC(1/4) KspI 31 CCGCGG CCGC^GG MamI 1258 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 893 TCCGGA T^CCGGA MvnI 32 CGCG CG^CG PpuMI 1143 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 812 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1192 ATTAAT AT^TAAT PvuII 968 CAGCTG CAG^CTG RsaI 1165 GTAC GT^AC SacII 31 CCGCGG CCGC^GG SfcI 1373 CTACAG C^T[GA][CT]AG SmaI 1252 CCCGGG CCC^GGG SmlI 491 CTCAAG C^T[CT][GA]AG SpeI 1353 ACTAGT A^CTAGT SphI 1481 GCATGC GCATG^C StuI 1491 AGGCCT AGG^CCT StyI 1711 CCAAGG C^C[AT][AT]GG XmaCI 1252 CCCGGG C^CCGGG XmaI 1252 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 887 ACATGT A^C[GA][CT]GT 1787 ACGTGT Asp700I 1513 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1603 GAATTTCTTC BanII 11 GAGCCC G[GA]GC[CT]^C 779 GAGCCC BfaI 983 CTAG C^TAG 1354 CTAG BmyI 11 GAGCCC G[AGT]GC[ACT]^C 779 GAGCCC BseRI 1618 CTCCTC GAGGAG(10/8) 8 GAGGAG BsmAI 819 GTCTC GTCTC(1/5) 1465 GTCTC BsmFI 810 GGGAC GGGAC(10/14) 1224 GTCCC Bsp1286I 11 GAGCCC G[AGT]GC[ACT]^C 779 GAGCCC BstBI 1520 TTCGAA TT^CGAA 1781 TTCGAA CfoI 357 GCGC GCG^C 1485 GCGC Cfr13I 45 GGCCC G^G[ACGT]CC 1144 GGTCC HgaI 829 GACGC GACGC(5/10) 1054 GCGTC HhaI 357 GCGC GCG^C 1485 GCGC HinP1I 357 GCGC G^CGC 1485 GCGC HphI 639 TCACC GGTGA(8/7) 1271 TCACC MaeI 983 CTAG C^TAG 1354 CTAG MaeII 1563 ACGT A^CGT 1787 ACGT MspA1I 31 CCGCGG C[AC]G^C[GT]G 968 CAGCTG NspI 887 ACATGT [GA]CATG^[CT] 1481 GCATGC NspV 1520 TTCGAA TT^CGAA 1781 TTCGAA PflMI 238 CCACAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 296 CCAAACTCTGG PleI 1554 GACTC GAGTC(4/5) 36 GAGTC Sau96I 45 GGCCC G^G[ACGT]CC 1144 GGTCC SfuI 1520 TTCGAA TT^CGAA 1781 TTCGAA Van91I 238 CCACAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 296 CCAAACTCTGG XmnI 1513 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1603 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BbvI BcnI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII