(Circular) (Six-base) MAPSORT of: pFN21AE1173.seq Check: 704 from: 1 to: 6523 pFN21AE1173 6523 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4333 278 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3866 1937 Size: 1929 4594 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2679 3016 4586 5970 6238 1632 Size: 534 513 337 1570 1384 268 1917 Fragments arranged by size: 1917 1570 1384 534 513 337 268 AfeI AGC'GCT Cuts at: 2363 2363 Size: 6523 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 2892 3792 5422 6399 1203 Size: 1689 900 1630 977 1327 Fragments arranged by size: 1689 1630 1327 977 900 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 3556 4266 5838 3556 Size: 710 1572 4241 Fragments arranged by size: 4241 1572 710 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 2108 2932 3331 3608 3832 4058 4210 6319 Size: 615 824 399 277 224 226 152 2109 Cuts at: 6319 1493 Size: 1697 Fragments arranged by size: 2109 1697 824 615 399 277 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 3198 5077 160 Size: 872 2166 1879 1606 Fragments arranged by size: 2166 1879 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 3257 3848 1870 Size: 73 1314 591 4545 Fragments arranged by size: 4545 1314 591 73 BamHI G'GATC_C Cuts at: 2716 3853 4220 2716 Size: 1137 367 5019 Fragments arranged by size: 5019 1137 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3844 5299 618 Size: 325 217 627 2057 1455 1842 Fragments arranged by size: 2057 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2788 3842 1391 Size: 629 768 1054 4072 Fragments arranged by size: 4072 1054 768 629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 928 Size: 971 665 4887 Fragments arranged by size: 4887 971 665 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2309 2343 4714 4748 1903 Size: 34 372 34 2371 34 3678 Fragments arranged by size: 3678 2371 372 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 1548 Size: 6523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5134 136 Size: 107 122 71 631 605 3462 1525 Fragments arranged by size: 3462 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6518 6518 Size: 6523 BlpI GC'TnA_GC Cuts at: 3940 3940 Size: 6523 Bme1580I G_kGCm'C Cuts at: 1790 4584 5740 1790 Size: 2794 1156 2573 Fragments arranged by size: 2794 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6523 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4254 5212 442 Size: 416 53 83 399 24 2837 958 1753 Fragments arranged by size: 2837 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3440 3440 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 3575 3731 5183 1239 Size: 918 1418 156 1452 2579 Fragments arranged by size: 2579 1452 1418 918 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2481 4648 5513 5811 2481 Size: 2167 865 298 3193 Fragments arranged by size: 3193 2167 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5186 882 Size: 617 3687 2219 Fragments arranged by size: 3687 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 3267 6517 3267 Size: 3250 3273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4330 4712 5347 275 Size: 53 83 186 936 2797 382 635 1451 Fragments arranged by size: 2797 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2898 4956 5354 5628 5775 6186 2898 Size: 2058 398 274 147 411 3235 Fragments arranged by size: 3235 2058 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6298 Fragments arranged by size: 6298 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2028 3614 1186 Size: 842 1586 4095 Fragments arranged by size: 4095 1586 842 BseYI C'CCAG_C Cuts at: 2028 3549 5726 6222 2028 Size: 1521 2177 496 2329 Fragments arranged by size: 2329 2177 1521 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5950 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3973 4734 5762 1540 Size: 275 186 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4584 4669 5740 4584 Size: 85 1071 5367 Fragments arranged by size: 5367 1071 85 BsmI GAATG_Cn' Cuts at: 2967 4034 4127 2967 Size: 1067 93 5363 Fragments arranged by size: 5363 1067 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2788 3842 4584 4669 5740 1391 Size: 399 230 768 1054 742 85 1071 2174 Fragments arranged by size: 2174 1071 1054 768 742 399 230 85 BspEI T'CCGG_A Cuts at: 2898 6186 2898 Size: 3288 3235 BspHI T'CATG_A Cuts at: 4412 6142 4412 Size: 1730 4793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3878 844 Size: 3034 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4410 1394 Size: 429 2587 3507 Fragments arranged by size: 3507 2587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2789 5018 5192 65 Size: 1183 1541 2229 174 1396 Fragments arranged by size: 2229 1541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5167 5354 1700 Size: 110 3357 187 2869 Fragments arranged by size: 3357 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 3526 3787 5327 3526 Size: 261 1540 4722 Fragments arranged by size: 4722 1540 261 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 3599 3599 Size: 6523 BstYI r'GATC_y Cuts at: 1972 2602 2716 3262 3853 4220 4605 4622 6074 Size: 630 114 546 591 367 385 17 1452 Cuts at: 6074 6160 6172 6518 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 630 591 546 385 367 346 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 513 Size: 552 939 32 5000 Fragments arranged by size: 5000 939 552 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5784 Fragments arranged by size: 5784 683 56 BtsI GCAGTG_nn' Cuts at: 2267 3244 4039 4833 4853 2267 Size: 977 795 794 20 3937 Fragments arranged by size: 3937 977 795 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3108 3970 4859 Size: 54 1425 50 275 18 1278 862 889 Cuts at: 4859 6488 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1278 889 862 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3431 4453 1353 Size: 2078 1022 3423 Fragments arranged by size: 3423 2078 1022 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2729 5110 5484 5630 387 Size: 1064 138 1140 2381 374 146 1280 Fragments arranged by size: 2381 1280 1140 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2166 2679 3016 3575 3731 4586 Size: 393 525 9 513 337 559 156 855 Cuts at: 4586 5183 5970 6238 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 855 787 597 559 525 513 393 337 268 156 9 EcoO109I rG'GnC_Cy Cuts at: 3149 3149 Size: 6523 EcoRI G'AATT_C Cuts at: 3832 4210 3832 Size: 378 6145 EcoRV GAT'ATC Cuts at: 2155 2155 Size: 6523 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3539 3539 Size: 6523 FspI TGC'GCA Cuts at: 3491 5029 3491 Size: 1538 4985 HaeII r_GCGC'y Cuts at: 1678 2365 5670 1678 Size: 687 3305 2531 Fragments arranged by size: 3305 2531 687 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3195 3227 5164 5196 5238 Size: 32 135 32 1623 32 1937 32 42 Cuts at: 5238 5270 1373 Size: 32 2626 Fragments arranged by size: 2626 1937 1623 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2890 3867 4113 669 Size: 988 281 952 977 246 3079 Fragments arranged by size: 3079 988 977 952 281 246 HindIII A'AGCT_T Cuts at: 748 3393 748 Size: 2645 3878 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 4122 4122 Size: 6523 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5637 5821 1305 Size: 4332 184 2007 Fragments arranged by size: 4332 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4701 Fragments arranged by size: 4701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3299 3659 4481 4840 4999 518 Size: 648 1644 489 360 822 359 159 2042 Fragments arranged by size: 2042 1644 822 648 489 360 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2975 3939 4616 5764 6009 1606 Size: 432 937 964 677 1148 245 2120 Fragments arranged by size: 2120 1148 964 937 677 432 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5032 Fragments arranged by size: 5032 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2896 3490 3881 5426 1132 Size: 97 1667 594 391 1545 2229 Fragments arranged by size: 2229 1667 1545 594 391 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6523 PciI A'CATG_T Cuts at: 2892 5422 2892 Size: 2530 3993 PflMI CCAn_nnn'nTGG Cuts at: 1108 2249 2307 1108 Size: 1141 58 5324 Fragments arranged by size: 5324 1141 58 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4592 4624 6105 6137 4592 Size: 32 1481 32 4978 Fragments arranged by size: 4978 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3149 3149 Size: 6523 PshAI GACnn'nnGTC Cuts at: 1613 2821 1613 Size: 1208 5315 PsiI TTA'TAA Cuts at: 4093 4093 Size: 6523 PstI C_TGCA'G Cuts at: 830 3875 830 Size: 3045 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2975 2975 Size: 6523 SacII CC_GC'GG Cuts at: 2039 2039 Size: 6523 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 4771 6483 4771 Size: 1712 4811 SfcI C'TryA_G Cuts at: 826 1045 3378 3871 5006 5687 5878 826 Size: 219 2333 493 1135 681 191 1471 Fragments arranged by size: 2333 1471 1135 681 493 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3259 3850 1872 Size: 1387 591 4545 Fragments arranged by size: 4545 1387 591 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 4627 5528 5790 820 Size: 197 926 553 2131 901 262 1553 Fragments arranged by size: 2131 1553 926 901 553 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 3358 152 Size: 3206 3317 SphI G_CATG'C Cuts at: 1132 3490 3881 1132 Size: 2358 391 3774 Fragments arranged by size: 3774 2358 391 SspI AAT'ATT Cuts at: 5 52 4447 5 Size: 47 4395 2081 Fragments arranged by size: 4395 2081 47 StuI AGG'CCT Cuts at: 1517 3498 1517 Size: 1981 4542 StyI C'CwwG_G Cuts at: 513 1065 2004 3716 3962 513 Size: 552 939 1712 246 3074 Fragments arranged by size: 3074 1712 939 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4550 4567 4720 4905 5350 325 Size: 1255 2970 17 153 185 445 1498 Fragments arranged by size: 2970 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3169 4769 6481 Size: 274 80 33 51 1443 1192 1600 1712 Cuts at: 6481 96 Size: 138 Fragments arranged by size: 1712 1600 1443 1192 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6483 6515 6483 Size: 32 6491 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6523 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3257 3848 1870 Size: 1387 591 4545 Fragments arranged by size: 4545 1387 591 XmnI GAAnn'nnTTC Cuts at: 3522 3612 4652 3522 Size: 90 1040 5393 Fragments arranged by size: 5393 1040 90 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4331 276 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI