Restriction map for
pFN21AB9863
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 1028 GAATTT [GA]^AATT[CT]
AflIII 569 ACATGT A^C[GA][CT]GT
AlwI 504 GATCC GGATC(4/5)
AlwNI 1015 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 713 GGGCCC GGGCC^C
ApoI 1028 GAATTT [GA]^AATT[CT]
Asp700I 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 214 GAGCAC G[AT]GC[AT]^C
AviII 1022 TGCGCA TGC^GCA
BalI 356 TGGCCA TGG^CCA
BbvCI 912 CCTCAGC CCTCAGC(-5/-2)
BcgI 795 GCATCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 151 CTAG C^TAG
BsiEI 1039 CGACCG CG[GA][CT]^CG
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
BsmAI 768 GAGAC GTCTC(1/5)
BsmBI 768 GAGACG CGTCTC(1/5)
BspDI 79 ATCGAT AT^CGAT
BspLU11I 569 ACATGT A^CATGT
BsrBI 1006 GAGCGG CCGCTC(-3/-3)
BsrFI 315 GCCGGC [GA]^CCGG[CT]
BstYI 503 AGATCC [GA]^GATC[CT]
Cfr10I 315 GCCGGC [GA]^CCGG[CT]
ClaI 79 ATCGAT AT^CGAT
DpnII 504 GATC ^GATC
DsaI 353 CCATGG C^C[GA][CT]GG
EarI 800 CTCTTC CTCTTC(1/4)
EcoNI 87 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 962 ATGCAT ATGCA^T
FspI 1022 TGCGCA TGC^GCA
HinfI 55 GACTC G^A[ACGT]TC
HphI 350 TCACC GGTGA(8/7)
Ksp632I 800 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MboI 504 GATC ^GATC
MflI 503 AGATCC [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MseI 500 TTAA T^TAA
MslI 348 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 315 GCCGGC GCC^GGC
NcoI 353 CCATGG C^CATGG
NdeII 504 GATC ^GATC
NgoMIV 315 GCCGGC G^CCGGC
NsiI 962 ATGCAT ATGCA^T
NspI 569 ACATGT [GA]CATG^[CT]
PleI 55 GACTC GAGTC(4/5)
Sau3AI 504 GATC ^GATC
SfiI 1235 GGCCTCGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 500 TTAA T^TAA
XhoII 503 AGATCC [GA]^GATC[CT]
XmnI 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
AfaI 336 GTAC GT^AC
1012 GTAC
AvaII 552 GGACC G^G[AT]CC
909 GGTCC
BanII 635 GAGCCC G[GA]GC[CT]^C
713 GGGCCC
BglI 521 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1236 GCCTCGTCGGC
BlpI 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
BsaHI 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
BsrI 542 ACTGG ACTGG(1/-1)
733 CCAGT
CelII 16 GCTGAGC GC^T[ACGT]AGC
390 GCTCAGC
EaeI 356 TGGCCA [CT]^GGCC[GA]
417 TGGCCG
HgaI 770 GACGC GACGC(5/10)
942 GCGTC
Hin1I 770 GACGCC G[GA]^CG[CT]C
941 GGCGTC
NlaIII 354 CATG CATG^
570 CATG
PstI 175 CTGCAG CTGCA^G
894 CTGCAG
RsaI 336 GTAC GT^AC
1012 GTAC
SfcI 175 CTGCAG C^T[GA][CT]AG
894 CTGCAG
Tsp509I 1029 AATT ^AATT
1145 AATT
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AvaI BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI SfaNI SmaI
StyI TaqI TseI TthHB8I XmaCI
XmaI