(Circular) (Six-base) MAPSORT of: pFN21AB9862.seq Check: 5935 from: 1 to: 5953 pFN21AB9862 5953 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2173 2655 3074 2173 Size: 482 419 5052 Fragments arranged by size: 5052 482 419 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2238 2325 4016 5400 5668 1632 Size: 606 87 1691 1384 268 1917 Fragments arranged by size: 1917 1691 1384 606 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 2574 4852 5829 1203 Size: 1371 2278 977 1327 Fragments arranged by size: 2278 1371 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 3025 3696 5268 3025 Size: 671 1572 3710 Fragments arranged by size: 3710 1572 671 ApaI G_GGCC'C Cuts at: 2722 2722 Size: 5953 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 3262 3488 3640 5749 1493 Size: 1769 226 152 2109 1697 Fragments arranged by size: 2109 1769 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2242 2635 2743 2768 3278 1870 Size: 73 271 28 393 108 25 510 4545 Fragments arranged by size: 4545 510 393 271 108 73 28 25 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2278 2845 2884 3227 Size: 325 217 627 477 14 567 39 343 Cuts at: 3227 3274 4729 618 Size: 47 1455 1842 Fragments arranged by size: 1842 1455 627 567 477 343 325 217 47 39 14 BanII G_rGCy'C Cuts at: 1391 2644 2722 3272 1391 Size: 1253 78 550 4072 Fragments arranged by size: 4072 1253 550 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 2118 2130 928 Size: 971 180 39 12 4751 Fragments arranged by size: 4751 971 180 39 12 BbvCI CC'TCA_GC Cuts at: 2918 2918 Size: 5953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2789 2823 4144 4178 1903 Size: 34 852 34 1321 34 3678 Fragments arranged by size: 3678 1321 852 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2532 3244 4564 Size: 107 122 71 631 605 860 712 1320 Cuts at: 4564 136 Size: 1525 Fragments arranged by size: 1525 1320 860 712 631 605 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 2022 2396 3370 2022 Size: 374 974 4605 Fragments arranged by size: 4605 974 374 Bme1580I G_kGCm'C Cuts at: 1790 2281 2722 2848 3230 4014 5170 1790 Size: 491 441 126 382 784 1156 2573 Fragments arranged by size: 2573 1156 784 491 441 382 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2569 3055 4613 1239 Size: 914 416 486 1558 2579 Fragments arranged by size: 2579 1558 914 486 416 Bpu10I CC'TnA_GC Cuts at: 2918 2918 Size: 5953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4078 4943 5241 4078 Size: 865 298 4790 Fragments arranged by size: 4790 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2776 2947 3760 4142 Size: 53 83 186 936 1243 171 813 382 Cuts at: 4142 4777 275 Size: 635 1451 Fragments arranged by size: 1451 1243 936 813 635 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 4552 Fragments arranged by size: 4552 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2144 2147 2150 2944 1186 Size: 928 30 3 3 794 4195 Fragments arranged by size: 4195 928 794 30 3 3 BseYI C'CCAG_C Cuts at: 3219 5156 5652 3219 Size: 1937 496 3520 Fragments arranged by size: 3520 1937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2658 2672 2777 1163 Size: 573 922 14 105 4339 Fragments arranged by size: 4339 922 573 105 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3047 3403 4164 5192 1540 Size: 275 186 1046 356 761 1028 2301 Fragments arranged by size: 2301 1046 1028 761 356 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 4014 4099 5170 2223 Size: 1791 85 1071 3006 Fragments arranged by size: 3006 1791 1071 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 BsmBI CGTCTCn'nnnn_ Cuts at: 2767 2767 Size: 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2281 2644 2722 2848 3230 3272 Size: 399 433 58 363 78 126 382 42 Cuts at: 3272 4014 4099 5170 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 382 363 126 85 78 58 42 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2019 2173 2655 2677 3074 3308 844 Size: 1175 154 482 22 397 234 3489 Fragments arranged by size: 3489 1175 482 397 234 154 22 BsrBI CCG'CTC Cuts at: 1394 1823 3013 3840 1394 Size: 429 1190 827 3507 Fragments arranged by size: 3507 1190 827 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 4597 4784 1700 Size: 110 510 2277 187 2869 Fragments arranged by size: 2869 2277 510 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2508 3283 3650 4035 4052 5504 5590 5602 Size: 536 775 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 775 536 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2358 513 Size: 552 1293 4108 Fragments arranged by size: 4108 1293 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 4111 Fragments arranged by size: 4111 1103 683 56 BtsI GCAGTG_nn' Cuts at: 3469 4263 4283 3469 Size: 794 20 5139 Fragments arranged by size: 5139 794 20 ClaI AT'CG_AT Cuts at: 1554 2085 1554 Size: 531 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2422 3400 Size: 54 1425 50 275 18 531 61 978 Cuts at: 3400 4289 5918 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 978 889 531 275 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2811 3883 1353 Size: 1458 1072 3423 Fragments arranged by size: 3423 1458 1072 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2462 2978 3117 4540 4914 5060 Size: 1064 138 873 516 139 1423 374 146 Cuts at: 5060 387 Size: 1280 Fragments arranged by size: 1423 1280 1064 873 516 374 146 139 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2238 2325 2569 3055 4016 4613 Size: 393 521 85 87 244 486 961 597 Cuts at: 4613 5400 5668 1239 Size: 787 268 1524 Fragments arranged by size: 1524 961 787 597 521 486 393 268 244 87 85 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 5953 EcoO109I rG'GnC_Cy Cuts at: 2705 2718 2719 2748 2705 Size: 13 1 29 5910 Fragments arranged by size: 5910 29 13 1 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 3029 4459 3029 Size: 1430 4523 HaeII r_GCGC'y Cuts at: 1678 2029 2389 2623 3250 5100 1678 Size: 351 360 234 627 1850 2531 Fragments arranged by size: 2531 1850 627 360 351 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 2549 2581 4594 Size: 32 135 32 636 32 309 32 2013 Cuts at: 4594 4626 4668 4700 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2013 636 309 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3297 3543 669 Size: 988 281 1359 246 3079 Fragments arranged by size: 3079 1359 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5067 5251 1305 Size: 3762 184 2007 Fragments arranged by size: 3762 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3600 Fragments arranged by size: 3600 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 3911 4270 4429 518 Size: 648 1191 1554 359 159 2042 Fragments arranged by size: 2042 1554 1191 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2400 3106 3136 3369 4046 5194 5439 1606 Size: 794 706 30 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 706 677 245 233 30 NaeI GCC'GGC Cuts at: 2322 2322 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2358 513 Size: 552 1293 4108 Fragments arranged by size: 4108 1293 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 2320 2320 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2578 3311 4856 1132 Size: 97 1349 733 1545 2229 Fragments arranged by size: 2229 1545 1349 733 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 2574 4852 2574 Size: 2278 3675 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PfoI T'CCnGG_A Cuts at: 2055 2055 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspOMI G'GGCC_C Cuts at: 2718 2718 Size: 5953 PstI C_TGCA'G Cuts at: 830 2184 2903 3305 830 Size: 1354 719 402 3478 Fragments arranged by size: 3478 1354 719 402 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SfcI C'TryA_G Cuts at: 826 1045 2180 2899 3301 4436 5117 5308 826 Size: 219 1135 719 402 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 719 681 402 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 2637 2745 2770 3280 1872 Size: 765 108 25 510 4545 Fragments arranged by size: 4545 765 510 108 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 4057 4958 5220 820 Size: 197 926 2114 901 262 1553 Fragments arranged by size: 2114 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2285 2358 2923 3206 3392 513 Size: 552 1220 73 565 283 186 3074 Fragments arranged by size: 3074 1220 565 552 283 186 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2544 2715 3980 3997 4150 4335 4780 Size: 1255 964 171 1265 17 153 185 445 Cuts at: 4780 325 Size: 1498 Fragments arranged by size: 1498 1265 1255 964 445 185 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4199 5911 96 Size: 274 80 33 51 1443 2222 1712 138 Fragments arranged by size: 2222 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 2635 2743 2768 3278 1870 Size: 765 108 25 510 4545 Fragments arranged by size: 4545 765 510 108 25 XmnI GAAnn'nnTTC Cuts at: 2612 4082 2612 Size: 1470 4483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI