################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccII AccIII AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI Bsp1407I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 428 GTAGAC GT^[AC][GT]AC AclI 317 AACGTT AA^CGTT AflIII 407 ACATGT A^C[GA][CT]GT AlwNI 109 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 706 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 740 CCCGGG C^[CT]CG[GA]G AvaII 370 GGACC G^G[AT]CC BanII 329 GAGCTC G[GA]GC[CT]^C BbsI 589 GAAGAC GAAGAC(2/6) BcgI 175 GCATGGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 589 GAAGAC GAAGAC(2/6) BsaBI 661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 73 GAGGAG GAGGAG(10/8) BsgI 281 GTGCAG GTGCAG(16/14) BsmFI 521 GTCCC GGGAC(10/14) BsmI 710 GCATTC GAATGC(1/-1) BsoBI 740 CCCGGG C^[CT]CG[GA]G BspLU11I 407 ACATGT A^CATGT BstBI 446 TTCGAA TT^CGAA EcoRV 850 GATATC GAT^ATC EcoT14I 611 CCTTGG C^C[AT][AT]GG HapII 741 CCGG C^CGG HpaII 741 CCGG C^CGG MaeII 318 ACGT A^CGT MamI 661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 424 TTAA T^TAA MspI 741 CCGG C^CGG NspI 407 ACATGT [GA]CATG^[CT] NspV 446 TTCGAA TT^CGAA Psp1406I 317 AACGTT AA^CGTT SacI 329 GAGCTC GAGCT^C SapI 237 GAAGAGC GCTCTTC(1/4) ScaI 22 AGTACT AGT^ACT SfuI 446 TTCGAA TT^CGAA SmaI 740 CCCGGG CCC^GGG StyI 611 CCTTGG C^C[AT][AT]GG Tru9I 424 TTAA T^TAA XmaCI 740 CCCGGG C^CCGGG XmaI 740 CCCGGG C^CCGGG XmnI 706 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AciI 761 CCGC CCGC(-3/-1) 127 GCGG AlwI 153 GATCC GGATC(4/5) 491 GGATC AspHI 180 GTGCTC G[AT]GC[AT]^C 329 GAGCTC BalI 158 TGGCCA TGG^CCA 830 TGGCCA BbvI 229 GCAGC GCAGC(8/12) 48 GCTGC BcnI 740 CCCGG CC^[GC]GG 741 CCGGG BfaI 70 CTAG C^TAG 563 CTAG BmyI 180 GTGCTC G[AGT]GC[ACT]^C 329 GAGCTC BsiHKAI 180 GTGCTC G[AT]GC[AT]^C 329 GAGCTC Bsp1286I 180 GTGCTC G[AGT]GC[ACT]^C 329 GAGCTC BspDI 121 ATCGAT AT^CGAT 385 ATCGAT BstYI 152 AGATCC [GA]^GATC[CT] 491 GGATCT Cac8I 763 GCACGC GC[ACGT]^[ACGT]GC 767 GCTTGC Cfr13I 370 GGACC G^G[ACGT]CC 738 GGCCC ClaI 121 ATCGAT AT^CGAT 385 ATCGAT EaeI 158 TGGCCA [CT]^GGCC[GA] 830 TGGCCA EarI 237 GAAGAG CTCTTC(1/4) 532 GAAGAG Fnu4HI 48 GCTGC GC^[ACGT]GC 229 GCAGC ItaI 48 GCTGC GC^[ACGT]GC 229 GCAGC Ksp632I 237 GAAGAG CTCTTC(1/4) 532 GAAGAG MaeI 70 CTAG C^TAG 563 CTAG MflI 152 AGATCC [GA]^GATC[CT] 491 GGATCT MluNI 158 TGGCCA TGG^CCA 830 TGGCCA MscI 158 TGGCCA TGG^CCA 830 TGGCCA MwoI 48 GCTGCAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 274 GCCAATCGTGC NciI 740 CCCGG CC^[GC]GG 741 CCGGG PstI 49 CTGCAG CTGCA^G 115 CTGCAG Sau96I 370 GGACC G^G[ACGT]CC 738 GGCCC SfaNI 124 GATGC GCATC(5/9) 65 GCATC SfcI 49 CTGCAG C^T[GA][CT]AG 115 CTGCAG TfiI 444 GATTC G^A[AT]TC 506 GATTC TseI 48 GCTGC G^C[AT]GC 229 GCAGC Tsp45I 39 GTCAC ^GT[GC]AC 724 GTGAC Tsp509I 11 AATT ^AATT 422 AATT XhoII 152 AGATCC [GA]^GATC[CT] 491 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AfaI AluI BsaJI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MnlI MvaI NdeII NlaIII PleI RsaI Sau3AI ScrFI TaqI TspRI TthHB8I