Restriction map for
pFN21AE3125
(Circular) (Six-base) MAPSORT of: pFN21AE3125.seq Check: 6437 from: 1 to: 5653
pFN21AE3125 5653 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2434 2996 1937
Size: 497 562 4594
Fragments arranged by size:
4594 562 497
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 2323 3780 4153 1563
Size: 760 1457 373 3063
Fragments arranged by size:
3063 1457 760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2901 2913 3716 5100 5368 1632
Size: 492 777 12 803 1384 268 1917
Fragments arranged by size:
1917 1384 803 777 492 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2412 4552 5529 1203
Size: 1209 2140 977 1327
Fragments arranged by size:
2140 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2145 1184
Size: 961 4692
AlwNI CAG_nnn'CTG
Cuts at: 2119 3396 4968 2119
Size: 1277 1572 2804
Fragments arranged by size:
2804 1572 1277
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 2978 1870
Size: 73 802 233 4545
Fragments arranged by size:
4545 802 233 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2338 2972 1391
Size: 947 634 4072
Fragments arranged by size:
4072 947 634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 928
Size: 971 702 3980
Fragments arranged by size:
3980 971 702
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2169 2203 3844 3878 1903
Size: 34 232 34 1641 34 3678
Fragments arranged by size:
3678 1641 232 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 3714 4870 1790
Size: 1924 1156 2573
Fragments arranged by size:
2573 1924 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 3384 4342
Size: 416 53 83 399 24 810 1157 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1157 958 810 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 2670 5647 2670
Size: 2977 2676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 1186
Size: 907 4746
BseYI C'CCAG_C
Cuts at: 2310 4856 5352 2310
Size: 2546 496 2611
Fragments arranged by size:
2611 2546 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2307 1163
Size: 573 571 4509
Fragments arranged by size:
4509 573 571
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2189 2338 3714 3799 4870 2189
Size: 149 1376 85 1071 2972
Fragments arranged by size:
2972 1376 1071 149 85
BsmI GAATG_Cn'
Cuts at: 2715 3164 3257 2715
Size: 449 93 5111
Fragments arranged by size:
5111 449 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2338 2972 3714 3799 4870 1391
Size: 399 399 149 634 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 399 399 149 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 2452 4457 2452
Size: 2005 3648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2157 2496 2983 3350 3735 3752 5204 5290
Size: 185 339 487 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 487 385 367 346 339 185
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4162
Fragments arranged by size:
4162 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 2127 2391 1554
Size: 573 264 4816
Fragments arranged by size:
4816 573 264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2835 3100
Size: 54 1425 50 275 18 333 672 265
Cuts at: 3100 3989 5618 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 333 275 265 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2532 3583 1353
Size: 884 295 1051 3423
Fragments arranged by size:
3423 1051 884 295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2336 2336
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2901 2913 3716 4313 5100 5368
Size: 393 492 777 12 803 597 787 268
Cuts at: 5368 1239
Size: 1524
Fragments arranged by size:
1524 803 787 777 597 492 393 268
12
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
EcoRV GAT'ATC
Cuts at: 2857 2857
Size: 5653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 2369 2114
Size: 255 5398
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2373 2405 2846 2878 4294
Size: 32 135 32 801 32 441 32 1416
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1416 801 441 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2837 10
Size: 54 1425 343 333 672 2826
Fragments arranged by size:
2826 1425 672 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3069 3746 4894 5139 1606
Size: 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4162
Fragments arranged by size:
4162 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2416 3011 4556 1132
Size: 97 1187 595 1545 2229
Fragments arranged by size:
2229 1545 1187 595 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 2412 4552 2412
Size: 2140 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4751
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2058 2124 3005 830
Size: 1228 66 881 3478
Fragments arranged by size:
3478 1228 881 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SacI G_AGCT'C
Cuts at: 2338 2338
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 2029 3901 5613 2029
Size: 1872 1712 2069
Fragments arranged by size:
2069 1872 1712
SfcI C'TryA_G
Cuts at: 826 1045 2054 2120 3001 4136 4817 5008 826
Size: 219 1009 66 881 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 881 681 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2747 2980 1872
Size: 875 233 4545
Fragments arranged by size:
4545 875 233
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2616 3092 513
Size: 552 939 612 476 3074
Fragments arranged by size:
3074 939 612 552 476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2027 3899 5611
Size: 274 80 33 51 1443 50 1872 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1872 1712 1443 274 138 80 51 50
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 5613 5645 2126
Size: 32 3455 32 2134
Fragments arranged by size:
3455 2134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2745 2978 1870
Size: 875 233 4545
Fragments arranged by size:
4545 875 233
XmnI GAAnn'nnTTC
Cuts at: 2715 3782 2715
Size: 1067 4586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI