################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AccIII AgeI AhdI Aor51HI AscI AspEI AspI AvrII BamHI BbeI BcgI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI HaeII HpaI KasI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1341 GGTACC G^GTACC AcyI 3937 GACGCC G[GA]^CG[CT]C AflII 609 CTTAAG C^TTAAG AflIII 69 ACGTGT A^C[GA][CT]GT Alw44I 3794 GTGCAC G^TGCAC AocI 2319 CCTGAGG CC^T[ACGT]AGG ApaI 54 GGGCCC GGGCC^C ApaLI 3794 GTGCAC G^TGCAC AseI 2151 ATTAAT AT^TAAT AsnI 2151 ATTAAT AT^TAAT Asp718I 1341 GGTACC G^GTACC AvaI 114 CTCGGG C^[CT]CG[GA]G AviII 101 TGCGCA TGC^GCA BanI 1341 GGTACC G^G[CT][GA]CC BbrPI 68 CACGTG CAC^GTG BclI 2372 TGATCA T^GATCA BcnI 299 CCCGG CC^[GC]GG BfrI 609 CTTAAG C^TTAAG BlpI 324 GCTGAGC GC^T[ACGT]AGC Bpu1102I 324 GCTGAGC GC^T[ACGT]AGC BsaAI 68 CACGTG [CT]AC^GT[GA] BsaHI 3937 GACGCC G[GA]^CG[CT]C BsaI 2209 GAGACC GGTCTC(1/5) BsgI 759 CTGCAC GTGCAG(16/14) BsmBI 394 CGTCTC CGTCTC(1/5) BsoBI 114 CTCGGG C^[CT]CG[GA]G BspMI 767 ACCTGC ACCTGC(4/8) BssHII 242 GCGCGC G^CGCGC BstAPI 993 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 294 GGTGACC G^GT[ACGT]ACC BstPI 294 GGTGACC G^GT[ACGT]ACC Bsu36I 2319 CCTGAGG CC^T[ACGT]AGG CelII 324 GCTGAGC GC^T[ACGT]AGC Eco81I 2319 CCTGAGG CC^T[ACGT]AGG EcoO65I 294 GGTGACC G^GT[ACGT]ACC EcoRI 619 GAATTC G^AATTC EcoRV 3405 GATATC GAT^ATC FbaI 2372 TGATCA T^GATCA FspI 101 TGCGCA TGC^GCA Hin1I 3937 GACGCC G[GA]^CG[CT]C HincII 2890 GTCAAC GT[CT]^[GA]AC HindII 2890 GTCAAC GT[CT]^[GA]AC KpnI 1341 GGTACC GGTAC^C NciI 299 CCCGG CC^[GC]GG NcoI 248 CCATGG C^CATGG PmaCI 68 CACGTG CAC^GTG PmlI 68 CACGTG CAC^GTG PshAI 2304 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2151 ATTAAT AT^TAAT SacI 1557 GAGCTC GAGCT^C StuI 110 AGGCCT AGG^CCT SwaI 2768 ATTTAAAT ATTT^AAAT XbaI 486 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### BalI 2426 TGGCCA TGG^CCA 3860 TGGCCA BanII 54 GGGCCC G[GA]GC[CT]^C 1557 GAGCTC BbvCI 237 CCTCAGC CCTCAGC(-5/-2) 2577 CCTCAGC BciVI 3233 GGATAC GTATCC(6/5) 3073 GTATCC Bsp1407I 684 TGTACA T^GTACA 4091 TGTACA BspHI 1490 TCATGA T^CATGA 2805 TCATGA BsrFI 124 GCCGGC [GA]^CCGG[CT] 194 GCCGGC BsrGI 684 TGTACA T^GTACA 4091 TGTACA Cfr10I 124 GCCGGC [GA]^CCGG[CT] 194 GCCGGC DsaI 248 CCATGG C^C[GA][CT]GG 3229 CCACGG EaeI 2426 TGGCCA [CT]^GGCC[GA] 3860 TGGCCA EcoT22I 2345 ATGCAT ATGCA^T 3394 ATGCAT HindIII 1365 AAGCTT A^AGCTT 3676 AAGCTT MfeI 3286 CAATTG C^AATTG 3572 CAATTG MluNI 2426 TGGCCA TGG^CCA 3860 TGGCCA MscI 2426 TGGCCA TGG^CCA 3860 TGGCCA MunI 3286 CAATTG C^AATTG 3572 CAATTG NaeI 124 GCCGGC GCC^GGC 194 GCCGGC NgoMIV 124 GCCGGC G^CCGGC 194 GCCGGC NsiI 2345 ATGCAT ATGCA^T 3394 ATGCAT PflMI 67 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3966 CCAGACCTTGG PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT] 3782 AGGTCCT PstI 991 CTGCAG CTGCA^G 1000 CTGCAG RcaI 1490 TCATGA T^CATGA 2805 TCATGA SpeI 711 ACTAGT A^CTAGT 4179 ACTAGT SspBI 684 TGTACA T^GTACA 4091 TGTACA SspI 2938 AATATT AAT^ATT 4017 AATATT Van91I 67 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3966 CCAGACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AclI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI Psp1406I PvuII RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI