Restriction map for
pFN21AB8888
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BseRI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 45 GTAC GT^AC
Alw44I 124 GTGCAC G^TGCAC
AlwI 303 GATCC GGATC(4/5)
ApaI 436 GGGCCC GGGCC^C
ApaLI 124 GTGCAC G^TGCAC
AspHI 124 GTGCAC G[AT]GC[AT]^C
AviII 293 TGCGCA TGC^GCA
BbvCI 546 CCTCAGC CCTCAGC(-5/-2)
BglII 530 AGATCT A^GATCT
BsaI 254 GAGACC GGTCTC(1/5)
BsgI 534 CTGCAC GTGCAG(16/14)
BsiEI 321 CGACCG CG[GA][CT]^CG
BsiHKAI 124 GTGCAC G[AT]GC[AT]^C
BsmBI 343 GAGACG CGTCTC(1/5)
BstAPI 536 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 530 AGATCT [GA]^GATC[CT]
DdeI 547 CTCAG C^T[ACGT]AG
DsaI 81 CCGCGG C^C[GA][CT]GG
EaeI 205 CGGCCA [CT]^GGCC[GA]
FseI 202 GGCCGGCC GGCCGG^CC
FspI 293 TGCGCA TGC^GCA
HgaI 345 GACGC GACGC(5/10)
HinfI 594 GATTC G^A[ACGT]TC
HphI 54 GGTGA GGTGA(8/7)
KspI 81 CCGCGG CCGC^GG
MaeII 386 ACGT A^CGT
MboII 446 TCTTC GAAGA(8/7)
MflI 530 AGATCT [GA]^GATC[CT]
RsaI 45 GTAC GT^AC
SacII 81 CCGCGG CCGC^GG
SfiI 202 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 404 AGGCCT AGG^CCT
TfiI 594 GATTC G^A[AT]TC
XhoII 530 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 24 GGCGCC G[GA]^CG[CT]C
151 GGCGCC
AvaI 49 CCCGAG C^[CT]CG[GA]G
96 CTCGGG
AvaII 78 GGACC G^G[AT]CC
573 GGACC
BanI 24 GGCGCC G^G[CT][GA]CC
151 GGCGCC
BbeI 24 GGCGCC GGCGC^C
151 GGCGCC
BpmI 145 CTCCAG CTGGAG(16/14)
360 CTCCAG
BsaHI 24 GGCGCC G[GA]^CG[CT]C
151 GGCGCC
BsmAI 254 GAGAC GTCTC(1/5)
343 GAGAC
BsoBI 49 CCCGAG C^[CT]CG[GA]G
96 CTCGGG
BsrFI 203 GCCGGC [GA]^CCGG[CT]
463 GCCGGC
Cfr10I 203 GCCGGC [GA]^CCGG[CT]
463 GCCGGC
DpnII 303 GATC ^GATC
531 GATC
Hin1I 24 GGCGCC G[GA]^CG[CT]C
151 GGCGCC
KasI 24 GGCGCC G^GCGCC
151 GGCGCC
MboI 303 GATC ^GATC
531 GATC
NaeI 203 GCCGGC GCC^GGC
463 GCCGGC
NarI 24 GGCGCC GG^CGCC
151 GGCGCC
NdeII 303 GATC ^GATC
531 GATC
NgoMIV 203 GCCGGC G^CCGGC
463 GCCGGC
Sau3AI 303 GATC ^GATC
531 GATC
SfoI 24 GGCGCC GGC^GCC
151 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI Bsp1286I BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI