(Circular) (Six-base) MAPSORT of: pFN21AB8888.seq Check: 9328 from: 1 to: 5308 pFN21AB8888 5308 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3118 278 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2651 1937 Size: 714 4594 Acc65I G'GTAC_C Cuts at: 2629 2629 Size: 5308 AclI AA'CG_TT Cuts at: 1563 3435 3808 1563 Size: 1872 373 3063 Fragments arranged by size: 3063 1872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 4755 5023 1632 Size: 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5111 AflIII A'CryG_T Cuts at: 1203 4207 5184 1203 Size: 3004 977 1327 Fragments arranged by size: 3004 1327 977 AgeI A'CCGG_T Cuts at: 4139 4139 Size: 5308 AhdI GACnn_n'nnGTC Cuts at: 4037 4037 Size: 5308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 1184 Size: 1227 4081 AlwNI CAG_nnn'CTG Cuts at: 3051 4623 3051 Size: 1572 3736 ApaI G_GGCC'C Cuts at: 2445 2445 Size: 5308 ApaLI G'TGCA_C Cuts at: 2129 3365 4521 2129 Size: 1236 1156 2916 Fragments arranged by size: 2916 1236 1156 ApoI r'AATT_y Cuts at: 1493 2617 2843 2995 5104 1493 Size: 1124 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 226 152 AseI AT'TA_AT Cuts at: 160 1032 3862 160 Size: 872 2830 1606 Fragments arranged by size: 2830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2101 2633 1870 Size: 73 111 47 532 4545 Fragments arranged by size: 4545 532 111 73 47 BamHI G'GATC_C Cuts at: 2638 3005 2638 Size: 367 4941 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2156 2629 4084 618 Size: 325 217 627 242 127 473 1455 1842 Fragments arranged by size: 1842 1455 627 473 325 242 217 127 BanII G_rGCy'C Cuts at: 1391 2066 2108 2226 2322 2445 2627 1391 Size: 675 42 118 96 123 182 4072 Fragments arranged by size: 4072 675 182 123 118 96 42 BbeI G_GCGC'C Cuts at: 2033 2160 2033 Size: 127 5181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4337 BbvCI CC'TCA_GC Cuts at: 2552 2552 Size: 5308 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3499 3533 1903 Size: 34 1562 34 3678 Fragments arranged by size: 3678 1562 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3200 4410 3200 Size: 1210 4098 BclI T'GATC_A Cuts at: 1548 1548 Size: 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 2214 2291 Size: 107 122 71 631 605 482 60 77 Cuts at: 2291 3919 136 Size: 1628 1525 Fragments arranged by size: 1628 1525 631 605 482 122 107 77 71 60 BglII A'GATC_T Cuts at: 2535 5303 2535 Size: 2768 2540 BlpI GC'TnA_GC Cuts at: 2725 2725 Size: 5308 Bme1580I G_kGCm'C Cuts at: 1790 2133 2445 3369 4525 1790 Size: 343 312 924 1156 2573 Fragments arranged by size: 2573 1156 924 343 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3039 3997 442 Size: 416 53 83 399 24 1622 958 1753 Fragments arranged by size: 1753 1622 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2350 3968 1239 Size: 896 215 1618 2579 Fragments arranged by size: 2579 1618 896 215 Bpu10I CC'TnA_GC Cuts at: 2552 2552 Size: 5308 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3433 4298 4596 3433 Size: 865 298 4145 Fragments arranged by size: 4145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 3971 882 Size: 617 754 1718 2219 Fragments arranged by size: 2219 1718 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3099 493 Size: 711 1895 2702 Fragments arranged by size: 2702 1895 711 BsaBI GATnn'nnATC Cuts at: 5302 5302 Size: 5308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2157 3115 3497 Size: 53 83 186 936 497 127 958 382 Cuts at: 3497 4132 275 Size: 635 1451 Fragments arranged by size: 1451 958 936 635 497 382 186 127 83 53 BsaWI w'CCGG_w Cuts at: 3741 4139 4413 4560 4971 3741 Size: 398 274 147 411 4078 Fragments arranged by size: 4078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5083 Fragments arranged by size: 5083 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5308 BseYI C'CCAG_C Cuts at: 2523 4511 5007 2523 Size: 1988 496 2824 Fragments arranged by size: 2824 1988 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2524 1163 Size: 573 788 3947 Fragments arranged by size: 3947 788 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2329 2758 3519 4547 1540 Size: 275 186 328 429 761 1028 2301 Fragments arranged by size: 2301 1028 761 429 328 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 3369 3454 4525 2133 Size: 1236 85 1071 2916 Fragments arranged by size: 2916 1236 1071 85 BsmI GAATG_Cn' Cuts at: 2819 2912 2819 Size: 93 5215 BsmBI CGTCTCn'nnnn_ Cuts at: 2342 2342 Size: 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2108 2133 2226 2322 2445 2627 Size: 399 276 42 25 93 96 123 182 Cuts at: 2627 3369 3454 4525 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 182 123 96 93 85 42 25 BspEI T'CCGG_A Cuts at: 4971 4971 Size: 5308 BspHI T'CATG_A Cuts at: 3197 4927 3197 Size: 1730 3578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 844 Size: 1819 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3195 1394 Size: 429 1372 3507 Fragments arranged by size: 3507 1372 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 3977 65 Size: 1183 2555 174 1396 Fragments arranged by size: 2555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2468 3952 4139 1700 Size: 110 398 260 1484 187 2869 Fragments arranged by size: 2869 1484 398 260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5308 BssHII G'CGCG_C Cuts at: 1925 2138 2249 2462 2464 1925 Size: 213 111 213 2 4769 Fragments arranged by size: 4769 213 213 111 2 BssSI C'ACGA_G Cuts at: 3368 4380 3368 Size: 1012 4296 BstAPI GCAn_nnn'nTGC Cuts at: 794 2547 794 Size: 1753 3555 BstBI TT'CG_AA Cuts at: 4112 4112 Size: 5308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5308 BstYI r'GATC_y Cuts at: 1972 2535 2638 3005 3390 3407 4859 4945 4957 Size: 563 103 367 385 17 1452 86 12 Cuts at: 4957 5303 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 563 385 367 346 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2086 513 Size: 552 1021 3735 Fragments arranged by size: 3735 1021 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4569 Fragments arranged by size: 4569 683 56 BtsI GCAGTG_nn' Cuts at: 2611 2824 3618 3638 2611 Size: 213 794 20 4281 Fragments arranged by size: 4281 794 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5308 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5273 DraI TTT'AAA Cuts at: 2612 2612 Size: 5308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4315 809 Size: 507 2999 1802 Fragments arranged by size: 2999 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2210 2755 3644 Size: 54 1425 50 275 18 380 545 889 Cuts at: 3644 5273 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 545 380 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2755 1537 Size: 275 943 4090 Fragments arranged by size: 4090 943 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3238 1353 Size: 1885 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 3895 4269 4415 387 Size: 1064 138 590 1716 374 146 1280 Fragments arranged by size: 1716 1280 1064 590 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2350 3371 3968 4755 5023 1239 Size: 393 503 215 1021 597 787 268 1524 Fragments arranged by size: 1524 1021 787 597 503 393 268 215 EcoRI G'AATT_C Cuts at: 2617 2995 2617 Size: 378 4930 FseI GG_CCGG'CC Cuts at: 2212 2212 Size: 5308 FspI TGC'GCA Cuts at: 2300 3814 2300 Size: 1514 3794 HaeII r_GCGC'y Cuts at: 1678 2033 2160 2297 4455 1678 Size: 355 127 137 2158 2531 Fragments arranged by size: 2531 2158 355 137 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3949 3981 4023 4055 1373 Size: 32 135 32 2377 32 42 32 2626 Fragments arranged by size: 2626 2377 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2652 2898 669 Size: 988 281 714 246 3079 Fragments arranged by size: 3079 988 714 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5308 HpaI GTT'AAC Cuts at: 2898 2898 Size: 5308 KasI G'GCGC_C Cuts at: 2029 2156 2029 Size: 127 5181 KpnI G_GTAC'C Cuts at: 2633 2633 Size: 5308 MfeI C'AATT_G Cuts at: 2907 2907 Size: 5308 MluI A'CGCG_T Cuts at: 5184 5184 Size: 5308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2052 2133 4422 4606 1305 Size: 747 81 2289 184 2007 Fragments arranged by size: 2289 2007 747 184 81 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3486 Fragments arranged by size: 3486 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 3625 3784 518 Size: 648 2100 359 159 2042 Fragments arranged by size: 2100 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2088 2233 2724 3401 4549 4794 1606 Size: 462 20 145 491 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 491 462 245 145 20 NaeI GCC'GGC Cuts at: 2210 2470 2210 Size: 260 5048 NarI GG'CG_CC Cuts at: 2030 2157 2030 Size: 127 5181 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4756 NdeI CA'TA_TG Cuts at: 387 387 Size: 5308 NgoMIV G'CCGG_C Cuts at: 2208 2468 2208 Size: 260 5048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5308 NotI GC'GGCC_GC Cuts at: 2755 2755 Size: 5308 NspI r_CATG'y Cuts at: 1132 1229 2666 4211 1132 Size: 97 1437 1545 2229 Fragments arranged by size: 2229 1545 1437 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5308 PciI A'CATG_T Cuts at: 4207 4207 Size: 5308 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5308 PmeI GTTT'AAAC Cuts at: 2612 2612 Size: 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 4890 4922 3377 Size: 32 1481 32 3763 Fragments arranged by size: 3763 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5308 PsiI TTA'TAA Cuts at: 2878 2878 Size: 5308 PspOMI G'GGCC_C Cuts at: 2441 2441 Size: 5308 PstI C_TGCA'G Cuts at: 830 2660 830 Size: 1830 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5308 SacII CC_GC'GG Cuts at: 2089 2089 Size: 5308 SalI G'TCGA_C Cuts at: 1936 2650 1936 Size: 714 4594 SbfI CC_TGCA'GG Cuts at: 2660 2660 Size: 5308 ScaI AGT'ACT Cuts at: 3556 5268 3556 Size: 1712 3596 SfcI C'TryA_G Cuts at: 826 1045 2656 3791 4472 4663 826 Size: 219 1611 1135 681 191 1471 Fragments arranged by size: 1611 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2214 2214 Size: 5308 SfoI GGC'GCC Cuts at: 2031 2158 2031 Size: 127 5181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5308 SmaI CCC'GGG Cuts at: 1872 2635 1872 Size: 763 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3412 4313 4575 820 Size: 197 926 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3099 493 Size: 2606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5308 SphI G_CATG'C Cuts at: 1132 2666 1132 Size: 1534 3774 SspI AAT'ATT Cuts at: 5 52 3232 5 Size: 47 3180 2081 Fragments arranged by size: 3180 2081 47 StuI AGG'CCT Cuts at: 1517 2411 1517 Size: 894 4414 StyI C'CwwG_G Cuts at: 513 1065 2747 513 Size: 552 1682 3074 Fragments arranged by size: 3074 1682 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 3335 3352 3505 3690 4135 325 Size: 1255 957 798 17 153 185 445 1498 Fragments arranged by size: 1498 1255 957 798 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3554 5266 96 Size: 274 80 33 51 1443 1577 1712 138 Fragments arranged by size: 1712 1577 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5268 5300 5268 Size: 32 5276 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5308 XbaI T'CTAG_A Cuts at: 2644 2644 Size: 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5308 XmaI C'CCGG_G Cuts at: 1870 2633 1870 Size: 763 4545 XmnI GAAnn'nnTTC Cuts at: 3437 3437 Size: 5308 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3116 276 Size: 53 83 186 936 1582 2468 Fragments arranged by size: 2468 1582 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI