(Linear) (Six-base) MAPSORT of: KIBB8888.seq Check: 3706 from: 1 to: 603 KIBB8888.orf With 182 enzymes: SgfI * December 5, 2014 12:54 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 406 603 Size: 406 197 ApaI G_GGCC'C Cuts at: 0 440 603 Size: 440 163 ApaLI G'TGCA_C Cuts at: 0 124 603 Size: 124 479 AvaI C'yCGr_G Cuts at: 0 49 96 603 Size: 49 47 507 Fragments arranged by size: 507 49 47 BanI G'GyrC_C Cuts at: 0 24 151 603 Size: 24 127 452 Fragments arranged by size: 452 127 24 BanII G_rGCy'C Cuts at: 0 61 103 221 317 440 603 Size: 61 42 118 96 123 163 Fragments arranged by size: 163 123 118 96 61 42 BbeI G_GCGC'C Cuts at: 0 28 155 603 Size: 28 127 448 Fragments arranged by size: 448 127 28 BbvCI CC'TCA_GC Cuts at: 0 547 603 Size: 547 56 BglI GCCn_nnn'nGGC Cuts at: 0 149 209 286 603 Size: 149 60 77 317 Fragments arranged by size: 317 149 77 60 BglII A'GATC_T Cuts at: 0 530 603 Size: 530 73 Bme1580I G_kGCm'C Cuts at: 0 128 440 603 Size: 128 312 163 Fragments arranged by size: 312 163 128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 345 603 Size: 130 215 258 Fragments arranged by size: 258 215 130 Bpu10I CC'TnA_GC Cuts at: 0 547 603 Size: 547 56 BsaI GGTCTCn'nnnn_ Cuts at: 0 248 603 Size: 248 355 BsaHI Gr'CG_yC Cuts at: 0 25 152 603 Size: 25 127 451 Fragments arranged by size: 451 127 25 BseYI C'CCAG_C Cuts at: 0 518 603 Size: 518 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 519 603 Size: 519 84 BsiEI CG_ry'CG Cuts at: 0 324 603 Size: 324 279 BsiHKAI G_wGCw'C Cuts at: 0 128 603 Size: 128 475 BsmBI CGTCTCn'nnnn_ Cuts at: 0 337 603 Size: 337 266 Bsp1286I G_dGCh'C Cuts at: 0 61 103 128 221 317 440 603 Size: 61 42 25 93 96 123 163 Fragments arranged by size: 163 123 96 93 61 42 25 BsrFI r'CCGG_y Cuts at: 0 203 463 603 Size: 203 260 140 Fragments arranged by size: 260 203 140 BssHII G'CGCG_C Cuts at: 0 133 244 457 459 603 Size: 133 111 213 2 144 Fragments arranged by size: 213 144 133 111 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 542 603 Size: 542 61 BstYI r'GATC_y Cuts at: 0 530 603 Size: 530 73 BtgI C'CryG_G Cuts at: 0 81 603 Size: 81 522 EaeI y'GGCC_r Cuts at: 0 205 603 Size: 205 398 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 174 603 Size: 174 429 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 345 603 Size: 130 215 258 Fragments arranged by size: 258 215 130 FseI GG_CCGG'CC Cuts at: 0 207 603 Size: 207 396 FspI TGC'GCA Cuts at: 0 295 603 Size: 295 308 HaeII r_GCGC'y Cuts at: 0 28 155 292 603 Size: 28 127 137 311 Fragments arranged by size: 311 137 127 28 KasI G'GCGC_C Cuts at: 0 24 151 603 Size: 24 127 452 Fragments arranged by size: 452 127 24 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 47 128 603 Size: 47 81 475 Fragments arranged by size: 475 81 47 MspA1I CmG'CkG Cuts at: 0 63 83 228 603 Size: 63 20 145 375 Fragments arranged by size: 375 145 63 20 NaeI GCC'GGC Cuts at: 0 205 465 603 Size: 205 260 138 Fragments arranged by size: 260 205 138 NarI GG'CG_CC Cuts at: 0 25 152 603 Size: 25 127 451 Fragments arranged by size: 451 127 25 NgoMIV G'CCGG_C Cuts at: 0 203 463 603 Size: 203 260 140 Fragments arranged by size: 260 203 140 PspOMI G'GGCC_C Cuts at: 0 436 603 Size: 436 167 SacII CC_GC'GG Cuts at: 0 84 603 Size: 84 519 SfiI GGCCn_nnn'nGGCC Cuts at: 0 209 603 Size: 209 394 SfoI GGC'GCC Cuts at: 0 26 153 603 Size: 26 127 450 Fragments arranged by size: 450 127 26 StuI AGG'CCT Cuts at: 0 406 603 Size: 406 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 532 603 Size: 532 71 Enzymes that do cut: AlfI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I BpmI Bpu10I BsaI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BstAPI BstYI BtgI EaeI EciI Eco57MI FseI FspI HaeII KasI MmeI MspA1I NaeI NarI NgoMIV PspOMI SacII SfiI SfoI StuI TaqII Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI