Restriction map for
pFN21AE2701
##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AvrII BciVI BfaI BfrI BlnI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
CpoI DraI EcoT22I HpaI MaeI
MfeI MluI MunI NdeI NheI
NruI NsiI NspV PacI PmeI
PshBI Psp1406I PvuI RsrII SalI
SfuI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 521 GGTACC G^GTACC
AccIII 7217 TCCGGA T^CCGGA
AflIII 4797 ACACGT A^C[GA][CT]GT
Asp718I 521 GGTACC G^GTACC
AspI 13000 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 12751 GGATCC G^GATCC
BseAI 7217 TCCGGA T^CCGGA
BsiWI 3958 CGTACG C^GTACG
BsmI 4250 GCATTC GAATGC(1/-1)
Bsp1407I 10670 TGTACA T^GTACA
BspDI 12613 ATCGAT AT^CGAT
BspEI 7217 TCCGGA T^CCGGA
BspHI 9273 TCATGA T^CATGA
BsrGI 10670 TGTACA T^GTACA
ClaI 12613 ATCGAT AT^CGAT
EcoRI 1699 GAATTC G^AATTC
EcoRV 442 GATATC GAT^ATC
HindIII 7479 AAGCTT A^AGCTT
KpnI 521 GGTACC GGTAC^C
MroI 7217 TCCGGA T^CCGGA
MseI 971 TTAA T^TAA
NotI 5809 GCGGCCGC GC^GGCCGC
PflFI 13000 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 10005 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 9273 TCATGA T^CATGA
ScaI 9779 AGTACT AGT^ACT
SexAI 12902 ACCTGGT A^CC[AT]GGT
SphI 4187 GCATGC GCATG^C
SspBI 10670 TGTACA T^GTACA
TfiI 9858 GATTC G^A[AT]TC
Tru9I 971 TTAA T^TAA
Tth111I 13000 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 8707 GACGTC GACGT^C
12387 GACGTC
AccI 8683 GTCTAC GT^[AC][GT]AC
11083 GTCTAC
AcsI 1699 GAATTC [GA]^AATT[CT]
6514 AAATTC
AgeI 11917 ACCGGT A^CCGGT
12976 ACCGGT
AhdI 739 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11503 GACCAGCTGTC
ApoI 1699 GAATTC [GA]^AATT[CT]
6514 AAATTC
Asp700I 135 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
13059 GAAGGCCTTC
AspEI 739 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11503 GACCAGCTGTC
BclI 10853 TGATCA T^GATCA
12578 TGATCA
Eam1105I 739 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
11503 GACCAGCTGTC
FbaI 10853 TGATCA T^GATCA
12578 TGATCA
NcoI 11678 CCATGG C^CATGG
12111 CCATGG
NspI 4187 GCATGC [GA]CATG^[CT]
12701 ACATGC
PinAI 11917 ACCGGT A^CCGGT
12976 ACCGGT
Tsp509I 1700 AATT ^AATT
6515 AATT
XmnI 135 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
13059 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 169 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PaeR7I PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfiI SfoI SgrAI SmaI SmlI
Sse8387I StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI