(Circular) (Six-base) MAPSORT of: pFN21AE2701.seq Check: 8477 from: 1 to: 18346 pFN21AE2701 18346 bp With 182 enzymes: SgfI * August 18, 2017 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4444 6499 7759 8323 8542 8818 9850 12661 15320 Size: 2055 1260 564 219 276 1032 2811 2659 Cuts at: 15320 4444 Size: 7470 Fragments arranged by size: 7470 2811 2659 2055 1260 1032 564 276 219 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10716 14396 16156 278 Size: 53 83 186 936 9180 3680 1760 2468 Fragments arranged by size: 9180 3680 2468 1760 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 10689 13089 15689 1937 Size: 8752 2400 2600 4594 Fragments arranged by size: 8752 4594 2600 2400 Acc65I G'GTAC_C Cuts at: 2526 15667 2526 Size: 13141 5205 AclI AA'CG_TT Cuts at: 1563 16473 16846 1563 Size: 14910 373 3063 Fragments arranged by size: 14910 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2864 2990 3170 3500 4025 4178 4406 4775 Size: 1232 126 180 330 525 153 228 369 Cuts at: 4775 5420 7814 8375 8660 10133 11156 11384 11902 Size: 645 2394 561 285 1473 1023 228 518 Cuts at: 11902 12469 13973 14129 14387 15146 16409 17793 18061 Size: 567 1504 156 258 759 1263 1384 268 Cuts at: 18061 1632 Size: 1917 Fragments arranged by size: 2394 1917 1504 1473 1384 1263 1232 1023 759 645 567 561 525 518 369 330 285 268 258 228 228 180 156 153 126 AfeI AGC'GCT Cuts at: 4955 5531 5564 6497 6785 6902 8795 9142 9218 Size: 576 33 933 288 117 1893 347 76 Cuts at: 9218 9524 10613 11018 14990 15161 4955 Size: 306 1089 405 3972 171 8140 Fragments arranged by size: 8140 3972 1893 1089 933 576 405 347 306 288 171 117 76 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 18149 AflIII A'CryG_T Cuts at: 1203 6802 17245 18222 1203 Size: 5599 10443 977 1327 Fragments arranged by size: 10443 5599 1327 977 AgeI A'CCGG_T Cuts at: 13922 14981 17177 13922 Size: 1059 2196 15091 Fragments arranged by size: 15091 2196 1059 AhdI GACnn_n'nnGTC Cuts at: 2749 13513 17075 2749 Size: 10764 3562 4020 Fragments arranged by size: 10764 4020 3562 AleI CACnn'nnGTG Cuts at: 1166 2193 4476 5056 10037 10889 12671 1166 Size: 1027 2283 580 4981 852 1782 6841 Fragments arranged by size: 6841 4981 2283 1782 1027 852 580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6362 6738 9882 10433 10961 12410 14144 15401 Size: 5178 376 3144 551 528 1449 1734 1257 Cuts at: 15401 1184 Size: 4129 Fragments arranged by size: 5178 4129 3144 1734 1449 1257 551 528 376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2906 2907 2938 2939 2906 Size: 1 31 1 18313 Fragments arranged by size: 18313 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2536 2968 3586 4225 4639 4906 4909 4918 5764 Size: 432 618 639 414 267 3 9 846 Cuts at: 5764 6277 6436 6616 7129 7198 7261 7369 8209 Size: 513 159 180 513 69 63 108 840 Cuts at: 8209 8536 8566 8638 9073 9304 9457 9475 9592 Size: 327 30 72 435 231 153 18 117 Cuts at: 9592 9646 9730 9784 9805 9976 10165 10606 11002 Size: 54 84 54 21 171 189 441 396 Cuts at: 11002 11116 11362 11395 11812 12069 12223 12283 12583 Size: 114 246 33 417 257 154 60 300 Cuts at: 12583 12913 13114 14935 15124 15181 15277 16089 17661 Size: 330 201 1821 189 57 96 812 1572 Cuts at: 17661 2536 Size: 3221 Fragments arranged by size: 3221 1821 1572 846 840 812 639 618 513 513 441 435 432 417 414 396 330 327 300 267 257 246 231 201 189 189 180 171 159 154 153 117 114 108 96 84 72 69 63 60 57 54 54 33 30 21 18 9 3 ApaI G_GGCC'C Cuts at: 4502 5326 5416 6607 10286 11270 11515 11662 12263 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 12263 12811 13269 13997 14840 15629 4502 Size: 548 458 728 843 789 7219 Fragments arranged by size: 7219 3679 1191 984 843 824 789 728 601 548 458 245 147 90 ApaLI G'TGCA_C Cuts at: 4469 4929 5015 6441 10460 12437 16403 17559 4469 Size: 460 86 1426 4019 1977 3966 1156 5256 Fragments arranged by size: 5256 4019 3966 1977 1426 1156 460 86 ApoI r'AATT_y Cuts at: 1493 3704 8519 15655 15881 16033 18142 1493 Size: 2211 4815 7136 226 152 2109 1697 Fragments arranged by size: 7136 4815 2211 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 16900 160 Size: 872 15868 1606 Fragments arranged by size: 15868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 18346 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2254 2271 3548 3762 4847 5378 Size: 73 93 218 17 1277 214 1085 531 Cuts at: 5378 5609 8292 8385 10286 10628 10707 11270 11382 Size: 231 2683 93 1901 342 79 563 112 Cuts at: 11382 11481 11655 12263 12325 12605 12794 12983 13034 Size: 99 174 608 62 280 189 189 51 Cuts at: 13034 13268 13997 14441 14713 15495 15577 15601 15671 Size: 234 729 444 272 782 82 24 70 Cuts at: 15671 1870 Size: 4545 Fragments arranged by size: 4545 2683 1901 1277 1085 782 729 608 563 531 444 342 280 272 234 231 218 214 189 189 174 112 99 93 93 82 79 73 70 62 51 24 17 BamHI G'GATC_C Cuts at: 14756 15676 16043 14756 Size: 920 367 17059 Fragments arranged by size: 17059 920 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2337 2526 3073 3393 3543 Size: 325 217 627 550 189 547 320 150 Cuts at: 3543 3767 4576 4653 5663 6654 7112 7950 8070 Size: 224 809 77 1010 991 458 838 120 Cuts at: 8070 10945 11069 11192 11521 11598 13010 13593 13610 Size: 2875 124 123 329 77 1412 583 17 Cuts at: 13610 13796 14604 15290 15667 17122 618 Size: 186 808 686 377 1455 1842 Fragments arranged by size: 2875 1842 1455 1412 1010 991 838 809 808 686 627 583 550 547 458 377 329 325 320 224 217 189 186 150 124 123 120 77 77 17 BanII G_rGCy'C Cuts at: 1391 4029 4502 4971 5326 5379 5416 5610 6290 Size: 2638 473 469 355 53 37 194 680 Cuts at: 6290 6607 8392 8694 9190 9270 10050 10286 10992 Size: 317 1785 302 496 80 780 236 706 Cuts at: 10992 11062 11270 11515 11589 11662 11676 11772 11955 Size: 70 208 245 74 73 14 96 183 Cuts at: 11955 11983 12041 12263 12811 13076 13269 13524 13902 Size: 28 58 222 548 265 193 255 378 Cuts at: 13902 13997 14840 15204 15629 15665 1391 Size: 95 843 364 425 36 4072 Fragments arranged by size: 4072 2638 1785 843 780 706 680 548 496 473 469 425 378 364 355 317 302 265 255 245 236 222 208 194 193 183 96 95 80 74 73 70 58 53 37 36 28 14 BbeI G_GCGC'C Cuts at: 3547 5667 6658 7954 8074 14608 15294 3547 Size: 2120 991 1296 120 6534 686 6599 Fragments arranged by size: 6599 6534 2120 1296 991 686 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 3176 6773 7476 10068 12893 13810 Size: 971 315 962 3597 703 2592 2825 917 Cuts at: 13810 14396 928 Size: 586 4878 Fragments arranged by size: 4878 3597 2825 2592 971 962 917 703 586 315 BbvCI CC'TCA_GC Cuts at: 2736 7326 7754 7962 8274 8985 9144 9201 9768 Size: 4590 428 208 312 711 159 57 567 Cuts at: 9768 11972 12651 12951 13568 2736 Size: 2204 679 300 617 7514 Fragments arranged by size: 7514 4590 2204 711 679 617 567 428 312 300 208 159 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4682 4716 5104 5138 6163 6197 9250 Size: 34 2745 34 388 34 1025 34 3053 Cuts at: 9250 9284 10078 10112 10960 10994 16537 16571 1903 Size: 34 794 34 848 34 5543 34 3678 Fragments arranged by size: 5543 3678 3053 2745 1025 848 794 388 34 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 16238 17448 16238 Size: 1210 17136 BclI T'GATC_A Cuts at: 1548 12858 14583 1548 Size: 11310 1725 5311 Fragments arranged by size: 11310 5311 1725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3002 3681 4364 Size: 107 122 71 631 605 1330 679 683 Cuts at: 4364 4604 5360 5390 5900 6320 6408 6584 6611 Size: 240 756 30 510 420 88 176 27 Cuts at: 6611 6869 6923 7274 7316 7409 8126 8267 8657 Size: 258 54 351 42 93 717 141 390 Cuts at: 8657 8870 9830 11265 11303 11519 12078 12155 12245 Size: 213 960 1435 38 216 559 77 90 Cuts at: 12245 12296 12747 13160 13415 15065 15119 16957 136 Size: 51 451 413 255 1650 54 1838 1525 Fragments arranged by size: 1838 1650 1525 1435 1330 960 756 717 683 679 631 605 559 510 451 420 413 390 351 258 255 240 216 213 176 141 122 107 93 90 88 77 71 54 54 51 42 38 30 27 BglII A'GATC_T Cuts at: 2439 10359 15333 18341 2439 Size: 7920 4974 3008 2444 Fragments arranged by size: 7920 4974 3008 2444 BlpI GC'TnA_GC Cuts at: 4529 4866 6057 6333 6834 8723 9150 9363 11624 Size: 337 1191 276 501 1889 427 213 2261 Cuts at: 11624 13296 15763 4529 Size: 1672 2467 7112 Fragments arranged by size: 7112 2467 2261 1889 1672 1191 501 427 337 276 213 Bme1580I G_kGCm'C Cuts at: 1790 3078 3730 4473 4502 4560 4581 4933 5019 Size: 1288 652 743 29 58 21 352 86 Cuts at: 5019 5065 5326 5416 5473 6445 6607 6847 7429 Size: 46 261 90 57 972 162 240 582 Cuts at: 7429 8455 10286 10464 10479 11202 11270 11463 11515 Size: 1026 1831 178 15 723 68 193 52 Cuts at: 11515 11662 11935 12263 12441 12811 13013 13269 13997 Size: 147 273 328 178 370 202 256 728 Cuts at: 13997 14840 15225 15380 15629 16407 17563 1790 Size: 843 385 155 249 778 1156 2573 Fragments arranged by size: 2573 1831 1288 1156 1026 972 843 778 743 728 723 652 582 385 370 352 328 273 261 256 249 240 202 193 178 178 162 155 147 90 86 68 58 57 52 46 29 21 15 BmgBI CAC'GTC Cuts at: 1318 2725 2806 3370 4711 5518 5593 10066 10486 Size: 1407 81 564 1341 807 75 4473 420 Cuts at: 10486 12463 15286 1318 Size: 1977 2823 4378 Fragments arranged by size: 4473 4378 2823 1977 1407 1341 807 564 420 81 75 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5627 11977 14049 Size: 416 53 83 399 24 4210 6350 2072 Cuts at: 14049 16077 17035 442 Size: 2028 958 1753 Fragments arranged by size: 6350 4210 2072 2028 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 18346 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 3994 4897 6574 7357 8713 11695 12055 2269 Size: 1725 903 1677 783 1356 2982 360 8560 Fragments arranged by size: 8560 2982 1725 1677 1356 903 783 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 2542 2584 2672 2914 3062 3158 3197 Size: 1025 278 42 88 242 148 96 39 Cuts at: 3197 3575 3869 3955 4019 4304 4640 4811 5012 Size: 378 294 86 64 285 336 171 201 Cuts at: 5012 5225 5246 5279 5789 5840 6131 6638 6725 Size: 213 21 33 510 51 291 507 87 Cuts at: 6725 6800 6917 7106 7220 7391 7664 7742 7856 Size: 75 117 189 114 171 273 78 114 Cuts at: 7856 7862 7925 8048 8609 9128 9152 9326 9389 Size: 6 63 123 561 519 24 174 63 Cuts at: 9389 9572 9752 9827 10214 10442 10628 10985 11117 Size: 183 180 75 387 228 186 357 132 Cuts at: 11117 11573 12110 12191 12419 12605 13196 13484 13925 Size: 456 537 81 228 186 591 288 441 Cuts at: 13925 14153 14531 14776 14891 14960 15188 15416 17006 Size: 228 378 245 115 69 228 228 1590 Cuts at: 17006 1239 Size: 2579 Fragments arranged by size: 2579 1590 1025 591 561 537 519 510 507 456 441 387 378 378 357 336 294 291 288 285 278 273 245 242 228 228 228 228 228 213 201 189 186 186 183 180 174 171 171 148 132 123 117 115 114 114 96 88 87 86 81 78 75 75 69 64 63 63 51 42 39 33 24 21 6 Bpu10I CC'TnA_GC Cuts at: 2093 2736 5318 7326 7754 7962 8274 8391 8985 Size: 643 2582 2008 428 208 312 117 594 Cuts at: 8985 9144 9201 9768 11972 12651 12951 13568 13586 Size: 159 57 567 2204 679 300 617 18 Cuts at: 13586 2093 Size: 6853 Fragments arranged by size: 6853 2582 2204 2008 679 643 617 594 567 428 312 300 208 159 117 57 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3053 3365 5252 5324 5579 5903 6896 7532 7556 Size: 312 1887 72 255 324 993 636 24 Cuts at: 7556 8717 8924 8963 9143 9401 11981 13118 15314 Size: 1161 207 39 180 258 2580 1137 2196 Cuts at: 15314 16471 17336 17634 3053 Size: 1157 865 298 3765 Fragments arranged by size: 3765 2580 2196 1887 1161 1157 1137 993 865 636 324 312 298 258 255 207 180 72 39 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2964 8588 11495 11693 11885 12309 12656 Size: 617 1465 5624 2907 198 192 424 347 Cuts at: 12656 17009 882 Size: 4353 2219 Fragments arranged by size: 5624 4353 2907 2219 1465 617 424 347 198 192 BsaAI yAC'GTr Cuts at: 493 1204 3127 4666 5953 6805 10593 12570 12931 Size: 711 1923 1539 1287 852 3788 1977 361 Cuts at: 12931 13543 16137 493 Size: 612 2594 2702 Fragments arranged by size: 3788 2702 2594 1977 1923 1539 1287 852 711 612 361 BsaBI GATnn'nnATC Cuts at: 2948 10880 12863 14549 18340 2948 Size: 7932 1983 1686 3791 2954 Fragments arranged by size: 7932 3791 2954 1983 1686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2784 3252 3544 5445 Size: 53 83 186 936 1251 468 292 1901 Cuts at: 5445 5664 5679 6655 7951 8071 9210 10713 11409 Size: 219 15 976 1296 120 1139 1503 696 Cuts at: 11409 11535 13641 13827 14393 14605 15006 15258 15291 Size: 126 2106 186 566 212 401 252 33 Cuts at: 15291 15525 16153 16535 17170 275 Size: 234 628 382 635 1451 Fragments arranged by size: 2106 1901 1503 1451 1296 1251 1139 976 936 696 635 628 566 468 401 382 292 252 234 219 212 186 186 126 120 83 53 33 15 BsaWI w'CCGG_w Cuts at: 9222 13922 14901 14981 16779 17177 17451 17598 18009 Size: 4700 979 80 1798 398 274 147 411 Cuts at: 18009 9222 Size: 9559 Fragments arranged by size: 9559 4700 1798 979 411 398 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2275 2305 2540 Size: 30 165 30 816 30 26 30 235 Cuts at: 2540 2570 3095 3125 3878 3908 7130 7160 8081 Size: 30 525 30 753 30 3222 30 921 Cuts at: 8081 8111 10682 10712 11897 11927 12095 12125 12734 Size: 30 2571 30 1185 30 168 30 609 Cuts at: 12734 12764 13009 13039 14519 14549 14582 14612 14864 Size: 30 245 30 1480 30 33 30 252 Cuts at: 14864 14894 15161 15191 1178 Size: 30 267 30 4333 Fragments arranged by size: 4333 3222 2571 1480 1185 921 816 753 609 525 267 252 245 235 168 165 33 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3245 3617 3814 3926 3929 3932 4778 Size: 1768 291 372 197 112 3 3 846 Cuts at: 4778 5324 5345 5786 5816 6053 6056 6059 6089 Size: 546 21 441 30 237 3 3 30 Cuts at: 6089 6215 6233 6278 6371 6374 6467 6500 6635 Size: 126 18 45 93 3 93 33 135 Cuts at: 6635 6656 6842 6893 6965 7019 7058 7217 7220 Size: 21 186 51 72 54 39 159 3 Cuts at: 7220 7343 7346 7421 7661 7694 7790 7793 7952 Size: 123 3 75 240 33 96 3 159 Cuts at: 7952 7955 7979 8009 8012 8075 8291 8300 8384 Size: 3 24 30 3 63 216 9 84 Cuts at: 8384 8606 8639 8699 8702 8732 8822 8852 8912 Size: 222 33 60 3 30 90 30 60 Cuts at: 8912 9095 9218 9251 9440 9614 9677 9707 9731 Size: 183 123 33 189 174 63 30 24 Cuts at: 9731 9770 9785 9824 9857 10019 10529 10910 11114 Size: 39 15 39 33 162 510 381 204 Cuts at: 11114 11513 11684 11897 12506 12698 12701 12812 13400 Size: 399 171 213 609 192 3 111 588 Cuts at: 13400 13703 13775 13982 14186 14240 14306 14545 14548 Size: 303 72 207 204 54 66 239 3 Cuts at: 14548 14555 14737 14752 14755 14843 15386 15562 15565 Size: 7 182 15 3 88 543 176 3 Cuts at: 15565 1186 Size: 3967 Fragments arranged by size: 3967 1768 846 609 588 546 543 510 441 399 381 372 303 291 240 239 237 222 216 213 207 204 204 197 192 189 186 183 182 176 174 171 162 159 159 135 126 123 123 112 111 96 93 93 90 88 84 75 72 72 66 63 63 60 60 54 54 51 45 39 39 39 33 33 33 33 33 30 30 30 30 30 30 24 24 21 21 18 15 15 9 7 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3650 3667 4037 4399 4626 5064 5110 5215 5550 Size: 17 370 362 227 438 46 105 335 Cuts at: 5550 7692 7846 9213 9585 10541 11412 11441 11555 Size: 2142 154 1367 372 956 871 29 114 Cuts at: 11555 12034 12518 12810 13410 13523 14799 14975 17549 Size: 479 484 292 600 113 1276 176 2574 Cuts at: 17549 18045 3650 Size: 496 3951 Fragments arranged by size: 3951 2574 2142 1367 1276 956 871 600 496 484 479 438 372 370 362 335 292 227 176 154 114 113 105 46 29 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2414 2828 3107 3392 3419 3557 Size: 573 417 261 414 279 285 27 138 Cuts at: 3557 3671 4400 4488 4733 4766 4781 5670 6533 Size: 114 729 88 245 33 15 889 863 Cuts at: 6533 6744 7994 8198 8318 8729 8981 9239 9317 Size: 211 1250 204 120 411 252 258 78 Cuts at: 9317 9461 10277 13259 14938 15194 1163 Size: 144 816 2982 1679 256 4315 Fragments arranged by size: 4315 2982 1679 1250 889 863 816 729 573 417 414 411 285 279 261 258 256 252 245 211 204 144 138 120 114 88 78 33 27 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2575 2904 3943 7387 7818 7992 Size: 275 186 574 329 1039 3444 431 174 Cuts at: 7992 10314 10984 12838 13282 14025 14593 14650 15114 Size: 2322 670 1854 444 743 568 57 464 Cuts at: 15114 15589 15796 16557 17585 1540 Size: 475 207 761 1028 2301 Fragments arranged by size: 3444 2322 2301 1854 1039 1028 761 743 670 574 568 475 464 444 431 329 275 207 186 174 57 BsiHKAI G_wGCw'C Cuts at: 2094 4029 4473 4530 4933 4971 5019 5319 5379 Size: 1935 444 57 403 38 48 300 60 Cuts at: 5379 5610 6290 6445 8694 9270 9936 10050 10407 Size: 231 680 155 2249 576 666 114 357 Cuts at: 10407 10464 10992 11589 11676 11772 11955 12441 13902 Size: 57 528 597 87 96 183 486 1461 Cuts at: 13902 16407 16492 17563 2094 Size: 2505 85 1071 2877 Fragments arranged by size: 2877 2505 2249 1935 1461 1071 680 666 597 576 528 486 444 403 357 300 231 183 155 114 96 87 85 60 57 57 48 38 BsiWI C'GTAC_G Cuts at: 5963 5963 Size: 18346 BsmI GAATG_Cn' Cuts at: 6255 15857 15950 6255 Size: 9602 93 8651 Fragments arranged by size: 9602 8651 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2562 2773 4997 6701 7160 9077 9458 10625 11022 Size: 211 2224 1704 459 1917 381 1167 397 Cuts at: 11022 11350 12602 14438 14794 14825 14858 2562 Size: 328 1252 1836 356 31 33 6050 Fragments arranged by size: 6050 2224 1917 1836 1704 1252 1167 459 397 381 356 328 211 33 31 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 3078 3730 4029 4473 4502 4530 Size: 399 304 984 652 299 444 29 28 Cuts at: 4530 4560 4581 4933 4971 5019 5065 5319 5326 Size: 30 21 352 38 48 46 254 7 Cuts at: 5326 5379 5416 5473 5610 6290 6445 6607 6847 Size: 53 37 57 137 680 155 162 240 Cuts at: 6847 7429 8392 8455 8694 9190 9270 9936 10050 Size: 582 963 63 239 496 80 666 114 Cuts at: 10050 10286 10407 10464 10479 10992 11062 11202 11270 Size: 236 121 57 15 513 70 140 68 Cuts at: 11270 11463 11515 11589 11662 11676 11772 11935 11955 Size: 193 52 74 73 14 96 163 20 Cuts at: 11955 11983 12041 12263 12441 12811 13013 13076 13269 Size: 28 58 222 178 370 202 63 193 Cuts at: 13269 13524 13902 13997 14840 15204 15225 15380 15629 Size: 255 378 95 843 364 21 155 249 Cuts at: 15629 15665 16407 16492 17563 1391 Size: 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 984 963 843 742 680 666 652 582 513 496 444 399 378 370 364 352 304 299 255 254 249 240 239 236 222 202 193 193 178 163 162 155 155 140 137 121 114 96 95 85 80 74 73 70 68 63 63 58 57 57 53 52 48 46 38 37 36 30 29 28 28 21 21 20 15 14 7 BspEI T'CCGG_A Cuts at: 9222 18009 9222 Size: 8787 9559 BspHI T'CATG_A Cuts at: 11278 16235 17965 11278 Size: 4957 1730 11659 Fragments arranged by size: 11659 4957 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2910 3585 3594 3654 3807 4299 4444 5221 Size: 2066 675 9 60 153 492 145 777 Cuts at: 5221 5967 6499 6679 7759 8323 8542 8622 8818 Size: 746 532 180 1080 564 219 80 196 Cuts at: 8818 9850 10086 10605 10833 11154 12661 13005 13101 Size: 1032 236 519 228 321 1507 344 96 Cuts at: 13101 13696 14316 15320 15701 844 Size: 595 620 1004 381 3489 Fragments arranged by size: 3489 2066 1507 1080 1032 1004 777 746 675 620 595 564 532 519 492 381 344 321 236 228 219 196 180 153 145 96 80 60 9 BsrBI CCG'CTC Cuts at: 1394 1823 2122 2686 3050 3142 3724 4725 5512 Size: 429 299 564 364 92 582 1001 787 Cuts at: 5512 5542 6085 6166 6652 6838 6868 6889 7501 Size: 30 543 81 486 186 30 21 612 Cuts at: 7501 7726 7945 8041 8087 8350 9118 11638 12589 Size: 225 219 96 46 263 768 2520 951 Cuts at: 12589 13225 13300 14386 14598 14652 14736 16233 1394 Size: 636 75 1086 212 54 84 1497 3507 Fragments arranged by size: 3507 2520 1497 1086 1001 951 787 768 636 612 582 564 543 486 429 364 299 263 225 219 212 186 96 92 84 81 75 54 46 30 30 21 BsrDI GCAATG_nn' Cuts at: 65 1248 16841 17015 65 Size: 1183 15593 174 1396 Fragments arranged by size: 15593 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2598 2637 4044 4854 5047 5359 5887 Size: 110 788 39 1407 810 193 312 528 Cuts at: 5887 6342 7382 7386 9986 10239 10494 10914 11189 Size: 455 1040 4 2600 253 255 420 275 Cuts at: 11189 11569 11601 12077 12305 12833 13310 13421 13613 Size: 380 32 476 228 528 477 111 192 Cuts at: 13613 13922 14181 14675 14723 14981 15152 15185 15476 Size: 309 259 494 48 258 171 33 291 Cuts at: 15476 15488 15500 15530 16990 17177 1700 Size: 12 12 30 1460 187 2869 Fragments arranged by size: 2869 2600 1460 1407 1040 810 788 528 528 494 477 476 455 420 380 312 309 291 275 259 258 255 253 228 193 192 187 171 111 110 48 39 33 32 30 12 12 4 BsrGI T'GTAC_A Cuts at: 96 12675 96 Size: 12579 5767 BssHII G'CGCG_C Cuts at: 1925 6587 6872 10318 14029 1925 Size: 4662 285 3446 3711 6242 Fragments arranged by size: 6242 4662 3711 3446 285 BssSI C'ACGA_G Cuts at: 3437 5327 10580 12233 12272 13259 16406 17418 3437 Size: 1890 5253 1653 39 987 3147 1012 4365 Fragments arranged by size: 5253 4365 3147 1890 1653 1012 987 39 BstAPI GCAn_nnn'nTGC Cuts at: 794 3535 3586 4225 4660 4909 5023 5575 5764 Size: 2741 51 639 435 249 114 552 189 Cuts at: 5764 6193 6277 6436 6440 8209 8227 8536 8959 Size: 429 84 159 4 1769 18 309 423 Cuts at: 8959 9439 9457 9475 11252 11395 12069 13803 14935 Size: 480 18 18 1777 143 674 1734 1132 Cuts at: 14935 794 Size: 4205 Fragments arranged by size: 4205 2741 1777 1769 1734 1132 674 639 552 480 435 429 423 309 249 189 159 143 114 84 51 18 18 18 4 BstBI TT'CG_AA Cuts at: 17150 17150 Size: 18346 BstEII G'GTnAC_C Cuts at: 1184 9286 10768 11839 12841 13306 13765 1184 Size: 8102 1482 1071 1002 465 459 5765 Fragments arranged by size: 8102 5765 1482 1071 1002 465 459 BstXI CCAn_nnnn'nTGG Cuts at: 2459 4037 5257 5281 5716 2459 Size: 1578 1220 24 435 15089 Fragments arranged by size: 15089 1578 1220 435 24 BstYI r'GATC_y Cuts at: 1972 2439 2949 5700 6300 6363 7782 9309 10359 Size: 467 510 2751 600 63 1419 1527 1050 Cuts at: 10359 14238 14756 15333 15676 16043 16428 16445 17897 Size: 3879 518 577 343 367 385 17 1452 Cuts at: 17897 17983 17995 18341 1972 Size: 86 12 346 1977 Fragments arranged by size: 3879 2751 1977 1527 1452 1419 1050 600 577 518 510 467 385 367 346 343 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2718 3839 10210 14169 2718 Size: 1121 6371 3959 6895 Fragments arranged by size: 6895 6371 3959 1121 BtgI C'CryG_G Cuts at: 513 1065 4077 5290 5785 7134 8652 9912 10035 Size: 552 3012 1213 495 1349 1518 1260 123 Cuts at: 10035 10437 10479 11166 11235 11421 11730 11844 12414 Size: 402 42 687 69 186 309 114 570 Cuts at: 12414 12742 13077 13660 13683 13722 14116 14148 14190 Size: 328 335 583 23 39 394 32 42 Cuts at: 14190 14841 15511 15639 513 Size: 651 670 128 3220 Fragments arranged by size: 3220 3012 1518 1349 1260 1213 687 670 651 583 570 552 495 402 394 335 328 309 186 128 123 114 69 42 42 39 32 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2232 2362 2554 5056 5157 9343 Size: 683 56 1006 130 192 2502 101 4186 Cuts at: 9343 11065 13792 14614 487 Size: 1722 2727 822 4219 Fragments arranged by size: 4219 4186 2727 2502 1722 1006 822 683 192 130 101 56 BtsI GCAGTG_nn' Cuts at: 4118 4235 6434 6727 8573 9849 11253 11790 12070 Size: 117 2199 293 1846 1276 1404 537 280 Cuts at: 12070 15862 16656 16676 4118 Size: 3792 794 20 5788 Fragments arranged by size: 5788 3792 2199 1846 1404 1276 794 537 293 280 117 20 ClaI AT'CG_AT Cuts at: 1554 14619 1554 Size: 13065 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3492 3527 14707 14742 529 Size: 35 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105 1089 43 432 56 151 889 1629 Cuts at: 18311 8 Size: 43 Fragments arranged by size: 1629 1425 1227 1182 1089 1002 889 812 652 624 612 581 432 422 408 405 391 357 329 303 302 294 275 260 238 228 199 189 183 174 151 144 105 91 78 76 75 60 60 56 54 50 43 43 40 39 33 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2572 2901 7384 7815 7989 10311 12835 Size: 275 760 329 4483 431 174 2322 2524 Cuts at: 12835 14022 15111 15586 15793 1537 Size: 1187 1089 475 207 4090 Fragments arranged by size: 4483 4090 2524 2322 1187 1089 760 475 431 329 275 207 174 EarI CTCTTCn'nnn_ Cuts at: 1353 2585 3365 4670 4932 6962 7431 7973 8148 Size: 1232 780 1305 262 2030 469 542 175 Cuts at: 8148 8334 8699 9566 10373 12350 12702 13355 14084 Size: 186 365 867 807 1977 352 653 729 Cuts at: 14084 14222 15562 16276 1353 Size: 138 1340 714 3423 Fragments arranged by size: 3423 2030 1977 1340 1305 1232 867 807 780 729 714 653 542 469 365 352 262 186 175 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2696 3353 4255 6176 6311 6464 Size: 1064 138 1107 657 902 1921 135 153 Cuts at: 6464 6473 6557 6653 6662 6746 6962 7025 7055 Size: 9 84 96 9 84 216 63 30 Cuts at: 7055 7121 7448 7511 7574 7601 7628 7844 7949 Size: 66 327 63 63 27 27 216 105 Cuts at: 7949 8165 8267 8297 8318 8474 8660 8849 9005 Size: 216 102 30 21 156 186 189 156 Cuts at: 9005 9092 9248 9970 10403 10550 12380 12527 12656 Size: 87 156 722 433 147 1830 147 129 Cuts at: 12656 12890 13036 13055 13868 14114 16933 17307 17453 Size: 234 146 19 813 246 2819 374 146 Cuts at: 17453 387 Size: 1280 Fragments arranged by size: 2819 1921 1830 1280 1107 1064 902 813 722 657 433 374 327 246 234 216 216 216 189 186 156 156 156 153 147 147 146 146 138 135 129 105 102 96 87 84 84 66 63 63 63 30 30 27 27 21 19 9 9 EcoICRI GAG'CTC Cuts at: 4027 4969 5377 5608 6288 8692 9268 10048 10990 Size: 942 408 231 680 2404 576 780 942 Cuts at: 10990 11587 11674 11770 11953 13900 4027 Size: 597 87 96 183 1947 8473 Fragments arranged by size: 8473 2404 1947 942 942 780 680 597 576 408 231 183 96 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2542 2584 2672 2864 2914 2990 Size: 393 632 278 42 88 192 50 76 Cuts at: 2990 3062 3158 3170 3197 3500 3575 3869 3955 Size: 72 96 12 27 303 75 294 86 Cuts at: 3955 4019 4025 4178 4304 4406 4640 4775 4811 Size: 64 6 153 126 102 234 135 36 Cuts at: 4811 5012 5225 5246 5279 5420 5789 5840 6131 Size: 201 213 21 33 141 369 51 291 Cuts at: 6131 6638 6725 6800 6917 7106 7220 7391 7664 Size: 507 87 75 117 189 114 171 273 Cuts at: 7664 7742 7814 7856 7862 7925 8048 8375 8609 Size: 78 72 42 6 63 123 327 234 Cuts at: 8609 8660 9128 9152 9326 9389 9572 9752 9827 Size: 51 468 24 174 63 183 180 75 Cuts at: 9827 10133 10214 10442 10628 10985 11117 11156 11384 Size: 306 81 228 186 357 132 39 228 Cuts at: 11384 11573 11902 12110 12191 12419 12469 12605 13196 Size: 189 329 208 81 228 50 136 591 Cuts at: 13196 13484 13925 13973 14129 14153 14387 14531 14776 Size: 288 441 48 156 24 234 144 245 Cuts at: 14776 14891 14960 15146 15188 15416 16409 17006 17793 Size: 115 69 186 42 228 993 597 787 Cuts at: 17793 18061 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 632 597 591 507 468 441 393 369 357 329 327 306 303 294 291 288 278 273 268 245 234 234 234 228 228 228 228 213 208 201 192 189 189 186 186 183 180 174 171 156 153 144 141 136 135 132 126 123 117 115 114 102 96 88 87 86 81 81 78 76 75 75 75 72 72 69 64 63 63 51 51 50 50 48 42 42 42 39 36 33 27 24 24 21 12 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 2174 2243 2663 4295 4922 5768 6662 6794 6977 Size: 69 420 1632 627 846 894 132 183 Cuts at: 6977 8804 9350 10193 10421 11354 11366 11495 11900 Size: 1827 546 843 228 933 12 129 405 Cuts at: 11900 12170 12347 12398 12767 12962 13025 13493 14081 Size: 270 177 51 369 195 63 468 588 Cuts at: 14081 14132 15128 15230 2174 Size: 51 996 102 5290 Fragments arranged by size: 5290 1827 1632 996 933 894 846 843 627 588 546 468 420 405 369 270 228 195 183 177 132 129 102 69 63 51 51 12 EcoO109I rG'GnC_Cy Cuts at: 2041 2097 2248 2533 2689 3583 3843 4288 4499 Size: 56 151 285 156 894 260 445 211 Cuts at: 4499 4636 5322 5761 6604 6613 8902 9472 10283 Size: 137 686 439 843 9 2289 570 811 Cuts at: 10283 10339 10387 10624 10822 10930 11267 11323 11359 Size: 56 48 237 198 108 337 56 36 Cuts at: 11359 11371 11392 11511 11512 11725 11968 12022 12136 Size: 12 21 119 1 213 243 54 114 Cuts at: 12136 12260 12364 12601 12955 13255 13265 13390 13744 Size: 124 104 237 354 300 10 125 354 Cuts at: 13744 13994 14050 14098 14485 14796 14837 15019 15085 Size: 250 56 48 387 311 41 182 66 Cuts at: 15085 15121 15235 15606 15625 15626 2041 Size: 36 114 371 19 1 4761 Fragments arranged by size: 4761 2289 894 843 811 686 570 445 439 387 371 354 354 337 311 300 285 260 250 243 237 237 213 211 198 182 156 151 137 125 124 119 114 114 108 104 66 56 56 56 56 54 48 48 41 36 36 21 19 12 10 9 1 1 EcoRI G'AATT_C Cuts at: 3704 15655 16033 3704 Size: 11951 378 6017 Fragments arranged by size: 11951 6017 378 EcoRV GAT'ATC Cuts at: 2449 2449 Size: 18346 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 7819 10579 12556 13672 14290 2161 Size: 5658 2760 1977 1116 618 6217 Fragments arranged by size: 6217 5658 2760 1977 1116 618 FseI GG_CCGG'CC Cuts at: 7386 12837 15156 7386 Size: 5451 2319 10576 Fragments arranged by size: 10576 5451 2319 FspI TGC'GCA Cuts at: 6350 7700 7883 8420 9021 10937 13535 14414 16852 Size: 1350 183 537 601 1916 2598 879 2438 Cuts at: 16852 6350 Size: 7844 Fragments arranged by size: 7844 2598 2438 1916 1350 879 601 537 183 HaeII r_GCGC'y Cuts at: 1678 3445 3547 3630 3986 4774 4957 5533 5566 Size: 1767 102 83 356 788 183 576 33 Cuts at: 5566 5667 5785 6403 6499 6658 6787 6904 6929 Size: 101 118 618 96 159 129 117 25 Cuts at: 6929 7129 7600 7687 7793 7954 8027 8074 8797 Size: 200 471 87 106 161 73 47 723 Cuts at: 8797 9144 9157 9220 9346 9526 10615 11020 11226 Size: 347 13 63 126 180 1089 405 206 Cuts at: 11226 12223 13567 13730 14422 14608 14992 15163 15294 Size: 997 1344 163 692 186 384 171 131 Cuts at: 15294 17493 1678 Size: 2199 2531 Fragments arranged by size: 2531 2199 1767 1344 1089 997 788 723 692 618 576 471 405 384 356 347 206 200 186 183 180 171 163 161 159 131 129 126 118 117 106 102 101 96 87 83 73 63 47 33 25 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 7319 7351 8081 Size: 32 135 32 1163 32 4552 32 730 Cuts at: 8081 8113 11897 11929 12095 12127 12308 12340 12964 Size: 32 3784 32 166 32 181 32 624 Cuts at: 12964 12996 13028 13060 13499 13531 14042 14074 14415 Size: 32 32 32 439 32 511 32 341 Cuts at: 14415 14447 14582 14614 14696 14728 14864 14896 16987 Size: 32 135 32 82 32 136 32 2091 Cuts at: 16987 17019 17061 17093 1373 Size: 32 42 32 2626 Fragments arranged by size: 4552 3784 2626 2091 1163 730 624 511 439 341 181 166 136 135 135 82 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 10255 15013 15690 15936 669 Size: 988 281 223 8094 4758 677 246 3079 Fragments arranged by size: 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CAynn'nnrTG Cuts at: 518 1166 2193 2621 3953 4476 5056 10037 10886 Size: 648 1027 428 1332 523 580 4981 849 Cuts at: 10886 10889 11165 12671 16304 16663 16822 518 Size: 3 276 1506 3633 359 159 2042 Fragments arranged by size: 4981 3633 2042 1506 1332 1027 849 648 580 523 428 359 276 159 3 MspA1I CmG'CkG Cuts at: 1606 2562 2839 3094 3238 3304 3667 4132 4324 Size: 956 277 255 144 66 363 465 192 Cuts at: 4324 4384 4492 4906 4990 5035 5292 5500 5554 Size: 60 108 414 84 45 257 208 54 Cuts at: 5554 5629 5716 5746 5860 6067 6121 6127 6277 Size: 75 87 30 114 207 54 6 150 Cuts at: 6277 6601 6778 6820 7063 7096 7102 7136 7189 Size: 324 177 42 243 33 6 34 53 Cuts at: 7189 7195 7453 7459 7486 7633 7696 7762 7820 Size: 6 258 6 27 147 63 66 58 Cuts at: 7820 7921 7927 8014 8110 8116 8239 8492 8587 Size: 101 6 87 96 6 123 253 95 Cuts at: 8587 8635 8890 8917 9073 9100 9124 9130 9454 Size: 48 255 27 156 27 24 6 324 Cuts at: 9454 9517 9592 9610 9643 9730 9742 9796 9914 Size: 63 75 18 33 87 12 54 118 Cuts at: 9914 10015 10162 10816 11002 11416 11596 11614 11803 Size: 101 147 654 186 414 180 18 189 Cuts at: 11803 11922 12280 12580 12805 12814 13414 13513 13582 Size: 119 358 300 225 9 600 99 69 Cuts at: 13582 13621 13735 13803 14314 14365 14803 14932 15447 Size: 39 114 68 511 51 438 129 515 Cuts at: 15447 15464 15513 15762 16439 17587 17832 1606 Size: 17 49 249 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 654 600 515 511 465 438 414 414 363 358 324 324 300 277 258 257 255 255 253 249 245 243 225 208 207 192 189 186 180 177 156 150 147 147 144 129 123 119 118 114 114 108 101 101 99 96 95 87 87 87 84 75 75 69 68 66 66 63 63 60 58 54 54 54 53 51 49 48 45 42 39 34 33 33 30 27 27 27 24 18 18 17 12 9 6 6 6 6 6 6 6 NaeI GCC'GGC Cuts at: 5049 5361 6344 7384 9988 10916 11191 11571 11603 Size: 312 983 1040 2604 928 275 380 32 Cuts at: 11603 12079 12835 14677 15154 15502 5049 Size: 476 756 1842 477 348 7893 Fragments arranged by size: 7893 2604 1842 1040 983 928 756 477 476 380 348 312 275 32 NarI GG'CG_CC Cuts at: 3544 5664 6655 7951 8071 14605 15291 3544 Size: 2120 991 1296 120 6534 686 6599 Fragments arranged by size: 6599 6534 2120 1296 991 686 120 NcoI C'CATG_G Cuts at: 513 1065 13683 14116 513 Size: 552 12618 433 4743 Fragments arranged by size: 12618 4743 552 433 NdeI CA'TA_TG Cuts at: 387 387 Size: 18346 NgoMIV G'CCGG_C Cuts at: 5047 5359 6342 7382 9986 10914 11189 11569 11601 Size: 312 983 1040 2604 928 275 380 32 Cuts at: 11601 12077 12833 14675 15152 15500 5047 Size: 476 756 1842 477 348 7893 Fragments arranged by size: 7893 2604 1842 1040 983 928 756 477 476 380 348 312 275 32 NheI G'CTAG_C Cuts at: 1052 1052 Size: 18346 NotI GC'GGCC_GC Cuts at: 7815 15793 7815 Size: 7978 10368 NspI r_CATG'y Cuts at: 1132 1229 6196 14710 15704 17249 1132 Size: 97 4967 8514 994 1545 2229 Fragments arranged by size: 8514 4967 2229 1545 994 97 PasI CC'CwG_GG Cuts at: 1348 15619 1348 Size: 14271 4075 PciI A'CATG_T Cuts at: 17245 17245 Size: 18346 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 3094 4495 4639 12817 1108 Size: 1932 54 1401 144 8178 6637 Fragments arranged by size: 8178 6637 1932 1401 144 54 PfoI T'CCnGG_A Cuts at: 2709 3135 5370 5943 9564 9804 10398 13002 13788 Size: 426 2235 573 3621 240 594 2604 786 Cuts at: 13788 13902 14792 14853 15165 2709 Size: 114 890 61 312 5890 Fragments arranged by size: 5890 3621 2604 2235 890 786 594 573 426 312 240 114 61 PmeI GTTT'AAAC Cuts at: 15650 15650 Size: 18346 PmlI CAC'GTG Cuts at: 3127 4666 6805 3127 Size: 1539 2139 14668 Fragments arranged by size: 14668 2139 1539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 12944 12976 16415 16447 17928 17960 12944 Size: 32 3439 32 1481 32 13330 Fragments arranged by size: 13330 3439 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2689 3583 3843 4288 8902 10339 10624 10822 Size: 441 894 260 445 4614 1437 285 198 Cuts at: 10822 11323 11725 11968 12022 12601 12955 14050 14796 Size: 501 402 243 54 579 354 1095 746 Cuts at: 14796 15085 15606 2248 Size: 289 521 4988 Fragments arranged by size: 4988 4614 1437 1095 894 746 579 521 501 445 441 402 354 289 285 260 243 198 54 PshAI GACnn'nnGTC Cuts at: 1613 12014 1613 Size: 10401 7945 PsiI TTA'TAA Cuts at: 15916 15916 Size: 18346 PspOMI G'GGCC_C Cuts at: 4498 5322 5412 6603 10282 11266 11511 11658 12259 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 12259 12807 13265 13993 14836 15625 4498 Size: 548 458 728 843 789 7219 Fragments arranged by size: 7219 3679 1191 984 843 824 789 728 601 548 458 245 147 90 PspXI vC'TCGA_Gb Cuts at: 5378 5609 5378 Size: 231 18115 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2618 2650 4157 4189 5993 6025 10577 10609 12554 Size: 32 1507 32 1804 32 4552 32 1945 Cuts at: 12554 12586 14288 14320 2618 Size: 32 1702 32 6644 Fragments arranged by size: 6644 4552 1945 1804 1702 1507 32 32 32 32 32 32 PstI C_TGCA'G Cuts at: 830 2307 2838 3210 3264 3289 3531 3582 3804 Size: 1477 531 372 54 25 242 51 222 Cuts at: 3804 4107 4230 4299 4889 4914 4989 5496 5676 Size: 303 123 69 590 25 75 507 180 Cuts at: 5676 5751 6186 6273 6384 6432 6612 6636 6741 Size: 75 435 87 111 48 180 24 105 Cuts at: 6741 6795 6945 7224 7240 8115 8148 8214 8410 Size: 54 150 279 16 875 33 66 196 Cuts at: 8410 8586 8634 8805 9003 9045 9414 9462 9480 Size: 176 48 171 198 42 369 48 18 Cuts at: 9480 9726 9801 9981 10014 10083 10155 10602 11007 Size: 246 75 180 33 69 72 447 405 Cuts at: 11007 11691 12060 13734 14313 15243 15698 830 Size: 684 369 1674 579 930 455 3478 Fragments arranged by size: 3478 1674 1477 930 875 684 590 579 531 507 455 447 435 405 372 369 369 303 279 246 242 222 198 196 180 180 180 176 171 150 123 111 105 87 75 75 75 72 69 69 66 54 54 51 48 48 48 42 33 33 25 25 24 18 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 18346 PvuII CAG'CTG Cuts at: 2562 2839 3238 3667 4132 4324 4384 4492 4906 Size: 277 399 429 465 192 60 108 414 Cuts at: 4906 4990 5554 5629 5746 5860 6127 6277 6778 Size: 84 564 75 117 114 267 150 501 Cuts at: 6778 6820 7102 7195 7459 7696 7927 8110 8116 Size: 42 282 93 264 237 231 183 6 Cuts at: 8116 8239 8492 8587 8890 9073 9130 9454 9592 Size: 123 253 95 303 183 57 324 138 Cuts at: 9592 9730 9796 10162 11002 11416 11614 12280 12580 Size: 138 66 366 840 414 198 666 300 Cuts at: 12580 12814 13414 13513 13582 13621 13735 13803 14314 Size: 234 600 99 69 39 114 68 511 Cuts at: 14314 14803 2562 Size: 489 6105 Fragments arranged by size: 6105 840 666 600 564 511 501 489 465 429 414 414 399 366 324 303 300 282 277 267 264 253 237 234 231 198 192 183 183 150 138 138 123 117 114 114 108 99 95 93 84 75 69 68 66 60 57 42 39 6 SacI G_AGCT'C Cuts at: 4029 4971 5379 5610 6290 8694 9270 10050 10992 Size: 942 408 231 680 2404 576 780 942 Cuts at: 10992 11589 11676 11772 11955 13902 4029 Size: 597 87 96 183 1947 8473 Fragments arranged by size: 8473 2404 1947 942 942 780 680 597 576 408 231 183 96 87 SacII CC_GC'GG Cuts at: 5293 7137 9915 15514 5293 Size: 1844 2778 5599 8125 Fragments arranged by size: 8125 5599 2778 1844 SalI G'TCGA_C Cuts at: 1936 15688 1936 Size: 13752 4594 SanDI GG'GwC_CC Cuts at: 11323 11323 Size: 18346 SapI GCTCTTCn'nnn_ Cuts at: 3365 4670 6962 7973 8699 9566 13355 3365 Size: 1305 2292 1011 726 867 3789 8356 Fragments arranged by size: 8356 3789 2292 1305 1011 867 726 SbfI CC_TGCA'GG Cuts at: 3582 6612 6795 8805 10083 15698 3582 Size: 3030 183 2010 1278 5615 6230 Fragments arranged by size: 6230 5615 3030 2010 1278 183 ScaI AGT'ACT Cuts at: 11786 16594 18306 11786 Size: 4808 1712 11826 Fragments arranged by size: 11826 4808 1712 SexAI A'CCwGG_T Cuts at: 14907 14907 Size: 18346 SfcI C'TryA_G Cuts at: 826 1045 2303 2834 3206 3260 3285 3527 3578 Size: 219 1258 531 372 54 25 242 51 Cuts at: 3578 3800 4103 4127 4226 4295 4885 4910 4985 Size: 222 303 24 99 69 590 25 75 Cuts at: 4985 5492 5672 5747 5971 6182 6269 6380 6428 Size: 507 180 75 224 211 87 111 48 Cuts at: 6428 6608 6632 6737 6791 6941 7220 7236 8111 Size: 180 24 105 54 150 279 16 875 Cuts at: 8111 8144 8198 8210 8406 8582 8630 8801 8999 Size: 33 54 12 196 176 48 171 198 Cuts at: 8999 9041 9410 9458 9476 9512 9722 9797 9977 Size: 42 369 48 18 36 210 75 180 Cuts at: 9977 10010 10079 10151 10598 11003 11545 11687 12056 Size: 33 69 72 447 405 542 142 369 Cuts at: 12056 12310 13730 14309 15239 15427 15436 15694 16829 Size: 254 1420 579 930 188 9 258 1135 Cuts at: 16829 17510 17701 826 Size: 681 191 1471 Fragments arranged by size: 1471 1420 1258 1135 930 875 681 590 579 542 531 507 447 405 372 369 369 303 279 258 254 242 224 222 219 211 210 198 196 191 188 180 180 180 176 171 150 142 111 105 99 87 75 75 75 72 69 69 54 54 54 51 48 48 48 42 36 33 33 25 25 24 24 18 16 12 9 SfiI GGCCn_nnn'nGGCC Cuts at: 4364 4604 5360 5390 5900 6611 7409 8126 8657 Size: 240 756 30 510 711 798 717 531 Cuts at: 8657 11265 15065 15119 4364 Size: 2608 3800 54 7591 Fragments arranged by size: 7591 3800 2608 798 756 717 711 531 510 240 54 30 SfoI GGC'GCC Cuts at: 3545 5665 6656 7952 8072 14606 15292 3545 Size: 2120 991 1296 120 6534 686 6599 Fragments arranged by size: 6599 6534 2120 1296 991 686 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 18346 SgrAI Cr'CCGG_yG Cuts at: 1700 13421 14723 14981 1700 Size: 11721 1302 258 5065 Fragments arranged by size: 11721 5065 1302 258 SmaI CCC'GGG Cuts at: 1872 2273 3764 8294 8387 10709 11384 15497 15673 Size: 401 1491 4530 93 2322 675 4113 176 Cuts at: 15673 1872 Size: 4545 Fragments arranged by size: 4545 4530 4113 2322 1491 675 401 176 93 SmlI C'TyrA_G Cuts at: 820 1017 1943 3068 3380 5267 5339 5378 5558 Size: 197 926 1125 312 1887 72 39 180 Cuts at: 5558 5609 5882 6911 7547 7571 8732 8939 8978 Size: 51 273 1029 636 24 1161 207 39 Cuts at: 8978 9158 9416 11996 12983 13034 13133 15329 16450 Size: 180 258 2580 987 51 99 2196 1121 Cuts at: 16450 17351 17613 820 Size: 901 262 1553 Fragments arranged by size: 2580 2196 1887 1553 1161 1125 1121 1029 987 926 901 636 312 273 262 258 207 197 180 180 99 72 51 51 39 39 24 SnaBI TAC'GTA Cuts at: 493 16137 493 Size: 15644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 18346 SphI G_CATG'C Cuts at: 1132 6196 15704 1132 Size: 5064 9508 3774 Fragments arranged by size: 9508 5064 3774 SrfI GCCC'GGGC Cuts at: 8387 8387 Size: 18346 SspI AAT'ATT Cuts at: 5 52 16270 5 Size: 47 16218 2081 Fragments arranged by size: 16218 2081 47 StuI AGG'CCT Cuts at: 1517 2669 3684 4353 4808 4997 5393 7625 11543 Size: 1152 1015 669 455 189 396 2232 3918 Cuts at: 11543 14474 14951 15068 1517 Size: 2931 477 117 4795 Fragments arranged by size: 4795 3918 2931 2232 1152 1015 669 477 455 396 189 117 StyI C'CwwG_G Cuts at: 513 1065 2404 2484 2997 3021 3828 4368 5388 Size: 552 1339 80 513 24 807 540 1020 Cuts at: 5388 5658 5913 6309 7278 8052 8922 9579 10554 Size: 270 255 396 969 774 870 657 975 Cuts at: 10554 10671 11525 11538 12099 12531 12846 13683 13833 Size: 117 854 13 561 432 315 837 150 Cuts at: 13833 14116 14265 15417 15785 513 Size: 283 149 1152 368 3074 Fragments arranged by size: 3074 1339 1152 1020 975 969 870 854 837 807 774 657 561 552 540 513 432 396 368 315 283 270 255 150 149 117 80 24 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3586 4413 5603 8612 9290 11723 14261 Size: 1255 2006 827 1190 3009 678 2433 2538 Cuts at: 14261 16373 16390 16543 16728 17173 325 Size: 2112 17 153 185 445 1498 Fragments arranged by size: 3009 2538 2433 2112 2006 1498 1255 1190 827 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3465 4332 5826 Size: 274 80 33 51 1443 1488 867 1494 Cuts at: 5826 6000 11784 12675 16592 18304 96 Size: 174 5784 891 3917 1712 138 Fragments arranged by size: 5784 3917 1712 1494 1488 1443 891 867 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3095 3127 3878 3910 9294 9326 12734 12766 13009 Size: 32 751 32 5384 32 3408 32 243 Cuts at: 13009 13041 14519 14551 18306 18338 3095 Size: 32 1478 32 3755 32 3103 Fragments arranged by size: 5384 3755 3408 3103 1478 751 243 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 15008 1316 Size: 13692 4654 XbaI T'CTAG_A Cuts at: 15682 15682 Size: 18346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3208 7366 9364 11953 12193 13690 14800 3208 Size: 4158 1998 2589 240 1497 1110 6754 Fragments arranged by size: 6754 4158 2589 1998 1497 1110 240 XhoI C'TCGA_G Cuts at: 1943 5378 5609 12983 13034 1943 Size: 3435 231 7374 51 7255 Fragments arranged by size: 7374 7255 3435 231 51 XmaI C'CCGG_G Cuts at: 1870 2271 3762 8292 8385 10707 11382 15495 15671 Size: 401 1491 4530 93 2322 675 4113 176 Cuts at: 15671 1870 Size: 4545 Fragments arranged by size: 4545 4530 4113 2322 1491 675 401 176 93 XmnI GAAnn'nnTTC Cuts at: 2144 15068 16475 2144 Size: 12924 1407 4015 Fragments arranged by size: 12924 4015 1407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10714 14394 16154 276 Size: 53 83 186 936 9180 3680 1760 2468 Fragments arranged by size: 9180 3680 2468 1760 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I FspAI NruI NsiI PacI RsrII SwaI