(Linear) (Six-base) MAPSORT of: KIEE2701.seq Check: 4438 from: 1 to: 13641 KIEE2701.orf With 182 enzymes: SgfI * August 18, 2017 10:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2439 4494 5754 6318 6537 6813 7845 10656 Size: 2439 2055 1260 564 219 276 1032 2811 Cuts at: 10656 13315 13641 Size: 2659 326 Fragments arranged by size: 2811 2659 2439 2055 1260 1032 564 326 276 219 AatII G_ACGT'C Cuts at: 0 8711 12391 13641 Size: 8711 3680 1250 Fragments arranged by size: 8711 3680 1250 AccI GT'mk_AC Cuts at: 0 8684 11084 13641 Size: 8684 2400 2557 Fragments arranged by size: 8684 2557 2400 Acc65I G'GTAC_C Cuts at: 0 521 13641 Size: 521 13120 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 859 985 1165 1495 2020 2173 2401 2770 Size: 859 126 180 330 525 153 228 369 Cuts at: 2770 3415 5809 6370 6655 8128 9151 9379 9897 Size: 645 2394 561 285 1473 1023 228 518 Cuts at: 9897 10464 11968 12124 12382 13141 13641 Size: 567 1504 156 258 759 500 Fragments arranged by size: 2394 1504 1473 1023 859 759 645 567 561 525 518 500 369 330 285 258 228 228 180 156 153 126 AfeI AGC'GCT Cuts at: 0 2950 3526 3559 4492 4780 4897 6790 7137 Size: 2950 576 33 933 288 117 1893 347 Cuts at: 7137 7213 7519 8608 9013 12985 13156 13641 Size: 76 306 1089 405 3972 171 485 Fragments arranged by size: 3972 2950 1893 1089 933 576 485 405 347 306 288 171 117 76 33 AflIII A'CryG_T Cuts at: 0 4797 13641 Size: 4797 8844 AgeI A'CCGG_T Cuts at: 0 11917 12976 13641 Size: 11917 1059 665 Fragments arranged by size: 11917 1059 665 AhdI GACnn_n'nnGTC Cuts at: 0 744 11508 13641 Size: 744 10764 2133 Fragments arranged by size: 10764 2133 744 AleI CACnn'nnGTG Cuts at: 0 188 2471 3051 8032 8884 10666 13641 Size: 188 2283 580 4981 852 1782 2975 Fragments arranged by size: 4981 2975 2283 1782 852 580 188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4357 4733 7877 8428 8956 10405 12139 13396 Size: 4357 376 3144 551 528 1449 1734 1257 Cuts at: 13396 13641 Size: 245 Fragments arranged by size: 4357 3144 1734 1449 1257 551 528 376 245 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 901 902 933 934 13641 Size: 901 1 31 1 12707 Fragments arranged by size: 12707 901 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 0 531 963 1581 2220 2634 2901 2904 2913 Size: 531 432 618 639 414 267 3 9 Cuts at: 2913 3759 4272 4431 4611 5124 5193 5256 5364 Size: 846 513 159 180 513 69 63 108 Cuts at: 5364 6204 6531 6561 6633 7068 7299 7452 7470 Size: 840 327 30 72 435 231 153 18 Cuts at: 7470 7587 7641 7725 7779 7800 7971 8160 8601 Size: 117 54 84 54 21 171 189 441 Cuts at: 8601 8997 9111 9357 9390 9807 10064 10218 10278 Size: 396 114 246 33 417 257 154 60 Cuts at: 10278 10578 10908 11109 12930 13119 13176 13272 13641 Size: 300 330 201 1821 189 57 96 369 Fragments arranged by size: 1821 846 840 639 618 531 513 513 441 435 432 417 414 396 369 330 327 300 267 257 246 231 201 189 189 180 171 159 154 153 117 114 108 96 84 72 69 63 60 57 54 54 33 30 21 18 9 3 ApaI G_GGCC'C Cuts at: 0 2497 3321 3411 4602 8281 9265 9510 9657 Size: 2497 824 90 1191 3679 984 245 147 Cuts at: 9657 10258 10806 11264 11992 12835 13624 13641 Size: 601 548 458 728 843 789 17 Fragments arranged by size: 3679 2497 1191 984 843 824 789 728 601 548 458 245 147 90 17 ApaLI G'TGCA_C Cuts at: 0 2464 2924 3010 4436 8455 10432 13641 Size: 2464 460 86 1426 4019 1977 3209 Fragments arranged by size: 4019 3209 2464 1977 1426 460 86 ApoI r'AATT_y Cuts at: 0 1699 6514 13641 Size: 1699 4815 7127 Fragments arranged by size: 7127 4815 1699 AvaI C'yCGr_G Cuts at: 0 31 249 266 1543 1757 2842 3373 3604 Size: 31 218 17 1277 214 1085 531 231 Cuts at: 3604 6287 6380 8281 8623 8702 9265 9377 9476 Size: 2683 93 1901 342 79 563 112 99 Cuts at: 9476 9650 10258 10320 10600 10789 10978 11029 11263 Size: 174 608 62 280 189 189 51 234 Cuts at: 11263 11992 12436 12708 13490 13572 13596 13641 Size: 729 444 272 782 82 24 45 Fragments arranged by size: 2683 1901 1277 1085 782 729 608 563 531 444 342 280 272 234 231 218 214 189 189 174 112 99 93 82 79 62 51 45 31 24 17 BamHI G'GATC_C Cuts at: 0 12751 13641 Size: 12751 890 BanI G'GyrC_C Cuts at: 0 332 521 1068 1388 1538 1762 2571 2648 Size: 332 189 547 320 150 224 809 77 Cuts at: 2648 3658 4649 5107 5945 6065 8940 9064 9187 Size: 1010 991 458 838 120 2875 124 123 Cuts at: 9187 9516 9593 11005 11588 11605 11791 12599 13285 Size: 329 77 1412 583 17 186 808 686 Cuts at: 13285 13641 Size: 356 Fragments arranged by size: 2875 1412 1010 991 838 809 808 686 583 547 458 356 332 329 320 224 189 186 150 124 123 120 77 77 17 BanII G_rGCy'C Cuts at: 0 2024 2497 2966 3321 3374 3411 3605 4285 Size: 2024 473 469 355 53 37 194 680 Cuts at: 4285 4602 6387 6689 7185 7265 8045 8281 8987 Size: 317 1785 302 496 80 780 236 706 Cuts at: 8987 9057 9265 9510 9584 9657 9671 9767 9950 Size: 70 208 245 74 73 14 96 183 Cuts at: 9950 9978 10036 10258 10806 11071 11264 11519 11897 Size: 28 58 222 548 265 193 255 378 Cuts at: 11897 11992 12835 13199 13624 13641 Size: 95 843 364 425 17 Fragments arranged by size: 2024 1785 843 780 706 680 548 496 473 469 425 378 364 355 317 302 265 255 245 236 222 208 194 193 183 96 95 80 74 73 70 58 53 37 28 17 14 BbeI G_GCGC'C Cuts at: 0 1542 3662 4653 5949 6069 12603 13289 13641 Size: 1542 2120 991 1296 120 6534 686 352 Fragments arranged by size: 6534 2120 1542 1296 991 686 352 120 BbsI GAAGACnn'nnnn_ Cuts at: 0 209 1171 4768 5471 8063 10888 11805 12391 Size: 209 962 3597 703 2592 2825 917 586 Cuts at: 12391 13641 Size: 1250 Fragments arranged by size: 3597 2825 2592 1250 962 917 703 586 209 BbvCI CC'TCA_GC Cuts at: 0 731 5321 5749 5957 6269 6980 7139 7196 Size: 731 4590 428 208 312 711 159 57 Cuts at: 7196 7763 9967 10646 10946 11563 13641 Size: 567 2204 679 300 617 2078 Fragments arranged by size: 4590 2204 2078 731 711 679 617 567 428 312 300 208 159 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2677 2711 3099 3133 4158 4192 7245 7279 Size: 2677 34 388 34 1025 34 3053 34 Cuts at: 7279 8073 8107 8955 8989 13641 Size: 794 34 848 34 4652 Fragments arranged by size: 4652 3053 2677 1025 848 794 388 34 34 34 34 34 34 BclI T'GATC_A Cuts at: 0 10853 12578 13641 Size: 10853 1725 1063 Fragments arranged by size: 10853 1725 1063 BglI GCCn_nnn'nGGC Cuts at: 0 997 1676 2359 2599 3355 3385 3895 4315 Size: 997 679 683 240 756 30 510 420 Cuts at: 4315 4403 4579 4606 4864 4918 5269 5311 5404 Size: 88 176 27 258 54 351 42 93 Cuts at: 5404 6121 6262 6652 6865 7825 9260 9298 9514 Size: 717 141 390 213 960 1435 38 216 Cuts at: 9514 10073 10150 10240 10291 10742 11155 11410 13060 Size: 559 77 90 51 451 413 255 1650 Cuts at: 13060 13114 13641 Size: 54 527 Fragments arranged by size: 1650 1435 997 960 756 717 683 679 559 527 510 451 420 413 390 351 258 255 240 216 213 176 141 93 90 88 77 54 54 51 42 38 30 27 BglII A'GATC_T Cuts at: 0 434 8354 13328 13641 Size: 434 7920 4974 313 Fragments arranged by size: 7920 4974 434 313 BlpI GC'TnA_GC Cuts at: 0 2524 2861 4052 4328 4829 6718 7145 7358 Size: 2524 337 1191 276 501 1889 427 213 Cuts at: 7358 9619 11291 13641 Size: 2261 1672 2350 Fragments arranged by size: 2524 2350 2261 1889 1672 1191 501 427 337 276 213 Bme1580I G_kGCm'C Cuts at: 0 1073 1725 2468 2497 2555 2576 2928 3014 Size: 1073 652 743 29 58 21 352 86 Cuts at: 3014 3060 3321 3411 3468 4440 4602 4842 5424 Size: 46 261 90 57 972 162 240 582 Cuts at: 5424 6450 8281 8459 8474 9197 9265 9458 9510 Size: 1026 1831 178 15 723 68 193 52 Cuts at: 9510 9657 9930 10258 10436 10806 11008 11264 11992 Size: 147 273 328 178 370 202 256 728 Cuts at: 11992 12835 13220 13375 13624 13641 Size: 843 385 155 249 17 Fragments arranged by size: 1831 1073 1026 972 843 743 728 723 652 582 385 370 352 328 273 261 256 249 240 202 193 178 178 162 155 147 90 86 68 58 57 52 46 29 21 17 15 BmgBI CAC'GTC Cuts at: 0 720 801 1365 2706 3513 3588 8061 8481 Size: 720 81 564 1341 807 75 4473 420 Cuts at: 8481 10458 13281 13641 Size: 1977 2823 360 Fragments arranged by size: 4473 2823 1977 1341 807 720 564 420 360 81 75 BmrI ACTGGGnnnn_n' Cuts at: 0 3622 9972 12044 13641 Size: 3622 6350 2072 1597 Fragments arranged by size: 6350 3622 2072 1597 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 264 1989 2892 4569 5352 6708 9690 10050 Size: 264 1725 903 1677 783 1356 2982 360 Cuts at: 10050 13641 Size: 3591 Fragments arranged by size: 3591 2982 1725 1677 1356 903 783 360 264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 537 579 667 909 1057 1153 1192 Size: 259 278 42 88 242 148 96 39 Cuts at: 1192 1570 1864 1950 2014 2299 2635 2806 3007 Size: 378 294 86 64 285 336 171 201 Cuts at: 3007 3220 3241 3274 3784 3835 4126 4633 4720 Size: 213 21 33 510 51 291 507 87 Cuts at: 4720 4795 4912 5101 5215 5386 5659 5737 5851 Size: 75 117 189 114 171 273 78 114 Cuts at: 5851 5857 5920 6043 6604 7123 7147 7321 7384 Size: 6 63 123 561 519 24 174 63 Cuts at: 7384 7567 7747 7822 8209 8437 8623 8980 9112 Size: 183 180 75 387 228 186 357 132 Cuts at: 9112 9568 10105 10186 10414 10600 11191 11479 11920 Size: 456 537 81 228 186 591 288 441 Cuts at: 11920 12148 12526 12771 12886 12955 13183 13411 13641 Size: 228 378 245 115 69 228 228 230 Fragments arranged by size: 591 561 537 519 510 507 456 441 387 378 378 357 336 294 291 288 285 278 273 259 245 242 230 228 228 228 228 228 213 201 189 186 186 183 180 174 171 171 148 132 123 117 115 114 114 96 88 87 86 81 78 75 75 69 64 63 63 51 42 39 33 24 21 6 Bpu10I CC'TnA_GC Cuts at: 0 88 731 3313 5321 5749 5957 6269 6386 Size: 88 643 2582 2008 428 208 312 117 Cuts at: 6386 6980 7139 7196 7763 9967 10646 10946 11563 Size: 594 159 57 567 2204 679 300 617 Cuts at: 11563 11581 13641 Size: 18 2060 Fragments arranged by size: 2582 2204 2060 2008 679 643 617 594 567 428 312 300 208 159 117 88 57 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1048 1360 3247 3319 3574 3898 4891 5527 Size: 1048 312 1887 72 255 324 993 636 Cuts at: 5527 5551 6712 6919 6958 7138 7396 9976 11113 Size: 24 1161 207 39 180 258 2580 1137 Cuts at: 11113 13309 13641 Size: 2196 332 Fragments arranged by size: 2580 2196 1887 1161 1137 1048 993 636 332 324 312 258 255 207 180 72 39 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 959 6583 9490 9688 9880 10304 10651 13641 Size: 959 5624 2907 198 192 424 347 2990 Fragments arranged by size: 5624 2990 2907 959 424 347 198 192 BsaAI yAC'GTr Cuts at: 0 1122 2661 3948 4800 8588 10565 10926 11538 Size: 1122 1539 1287 852 3788 1977 361 612 Cuts at: 11538 13641 Size: 2103 Fragments arranged by size: 3788 2103 1977 1539 1287 1122 852 612 361 BsaBI GATnn'nnATC Cuts at: 0 943 8875 10858 12544 13641 Size: 943 7932 1983 1686 1097 Fragments arranged by size: 7932 1983 1686 1097 943 BsaHI Gr'CG_yC Cuts at: 0 779 1247 1539 3440 3659 3674 4650 5946 Size: 779 468 292 1901 219 15 976 1296 Cuts at: 5946 6066 7205 8708 9404 9530 11636 11822 12388 Size: 120 1139 1503 696 126 2106 186 566 Cuts at: 12388 12600 13001 13253 13286 13520 13641 Size: 212 401 252 33 234 121 Fragments arranged by size: 2106 1901 1503 1296 1139 976 779 696 566 468 401 292 252 234 219 212 186 126 121 120 33 15 BsaWI w'CCGG_w Cuts at: 0 7217 11917 12896 12976 13641 Size: 7217 4700 979 80 665 Fragments arranged by size: 7217 4700 979 665 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 214 244 270 300 535 565 1090 1120 Size: 214 30 26 30 235 30 525 30 Cuts at: 1120 1873 1903 5125 5155 6076 6106 8677 8707 Size: 753 30 3222 30 921 30 2571 30 Cuts at: 8707 9892 9922 10090 10120 10729 10759 11004 11034 Size: 1185 30 168 30 609 30 245 30 Cuts at: 11034 12514 12544 12577 12607 12859 12889 13156 13186 Size: 1480 30 33 30 252 30 267 30 Cuts at: 13186 13641 Size: 455 Fragments arranged by size: 3222 2571 1480 1185 921 753 609 525 455 267 252 245 235 214 168 33 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 949 1240 1612 1809 1921 1924 1927 2773 Size: 949 291 372 197 112 3 3 846 Cuts at: 2773 3319 3340 3781 3811 4048 4051 4054 4084 Size: 546 21 441 30 237 3 3 30 Cuts at: 4084 4210 4228 4273 4366 4369 4462 4495 4630 Size: 126 18 45 93 3 93 33 135 Cuts at: 4630 4651 4837 4888 4960 5014 5053 5212 5215 Size: 21 186 51 72 54 39 159 3 Cuts at: 5215 5338 5341 5416 5656 5689 5785 5788 5947 Size: 123 3 75 240 33 96 3 159 Cuts at: 5947 5950 5974 6004 6007 6070 6286 6295 6379 Size: 3 24 30 3 63 216 9 84 Cuts at: 6379 6601 6634 6694 6697 6727 6817 6847 6907 Size: 222 33 60 3 30 90 30 60 Cuts at: 6907 7090 7213 7246 7435 7609 7672 7702 7726 Size: 183 123 33 189 174 63 30 24 Cuts at: 7726 7765 7780 7819 7852 8014 8524 8905 9109 Size: 39 15 39 33 162 510 381 204 Cuts at: 9109 9508 9679 9892 10501 10693 10696 10807 11395 Size: 399 171 213 609 192 3 111 588 Cuts at: 11395 11698 11770 11977 12181 12235 12301 12540 12543 Size: 303 72 207 204 54 66 239 3 Cuts at: 12543 12550 12732 12747 12750 12838 13381 13557 13560 Size: 7 182 15 3 88 543 176 3 Cuts at: 13560 13641 Size: 81 Fragments arranged by size: 949 846 609 588 546 543 510 441 399 381 372 303 291 240 239 237 222 216 213 207 204 204 197 192 189 186 183 182 176 174 171 162 159 159 135 126 123 123 112 111 96 93 93 90 88 84 81 75 72 72 66 63 63 60 60 54 54 51 45 39 39 39 33 33 33 33 33 30 30 30 30 30 30 24 24 21 21 18 15 15 9 7 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1645 1662 2032 2394 2621 3059 3105 3210 Size: 1645 17 370 362 227 438 46 105 Cuts at: 3210 3545 5687 5841 7208 7580 8536 9407 9436 Size: 335 2142 154 1367 372 956 871 29 Cuts at: 9436 9550 10029 10513 10805 11405 11518 12794 12970 Size: 114 479 484 292 600 113 1276 176 Cuts at: 12970 13641 Size: 671 Fragments arranged by size: 2142 1645 1367 1276 956 871 671 600 484 479 438 372 370 362 335 292 227 176 154 114 113 105 46 29 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 409 823 1102 1387 1414 1552 1666 Size: 148 261 414 279 285 27 138 114 Cuts at: 1666 2395 2483 2728 2761 2776 3665 4528 4739 Size: 729 88 245 33 15 889 863 211 Cuts at: 4739 5989 6193 6313 6724 6976 7234 7312 7456 Size: 1250 204 120 411 252 258 78 144 Cuts at: 7456 8272 11254 12933 13189 13641 Size: 816 2982 1679 256 452 Fragments arranged by size: 2982 1679 1250 889 863 816 729 452 414 411 285 279 261 258 256 252 245 211 204 148 144 138 120 114 88 78 33 27 15 BsiEI CG_ry'CG Cuts at: 0 570 899 1938 5382 5813 5987 8309 8979 Size: 570 329 1039 3444 431 174 2322 670 Cuts at: 8979 10833 11277 12020 12588 12645 13109 13584 13641 Size: 1854 444 743 568 57 464 475 57 Fragments arranged by size: 3444 2322 1854 1039 743 670 570 568 475 464 444 431 329 174 57 57 BsiHKAI G_wGCw'C Cuts at: 0 89 2024 2468 2525 2928 2966 3014 3314 Size: 89 1935 444 57 403 38 48 300 Cuts at: 3314 3374 3605 4285 4440 6689 7265 7931 8045 Size: 60 231 680 155 2249 576 666 114 Cuts at: 8045 8402 8459 8987 9584 9671 9767 9950 10436 Size: 357 57 528 597 87 96 183 486 Cuts at: 10436 11897 13641 Size: 1461 1744 Fragments arranged by size: 2249 1935 1744 1461 680 666 597 576 528 486 444 403 357 300 231 183 155 114 96 89 87 60 57 57 48 38 BsiWI C'GTAC_G Cuts at: 0 3958 13641 Size: 3958 9683 BsmI GAATG_Cn' Cuts at: 0 4250 13641 Size: 4250 9391 BsmBI CGTCTCn'nnnn_ Cuts at: 0 557 768 2992 4696 5155 7072 7453 8620 Size: 557 211 2224 1704 459 1917 381 1167 Cuts at: 8620 9017 9345 10597 12433 12789 12820 12853 13641 Size: 397 328 1252 1836 356 31 33 788 Fragments arranged by size: 2224 1917 1836 1704 1252 1167 788 557 459 397 381 356 328 211 33 31 Bsp1286I G_dGCh'C Cuts at: 0 89 1073 1725 2024 2468 2497 2525 2555 Size: 89 984 652 299 444 29 28 30 Cuts at: 2555 2576 2928 2966 3014 3060 3314 3321 3374 Size: 21 352 38 48 46 254 7 53 Cuts at: 3374 3411 3468 3605 4285 4440 4602 4842 5424 Size: 37 57 137 680 155 162 240 582 Cuts at: 5424 6387 6450 6689 7185 7265 7931 8045 8281 Size: 963 63 239 496 80 666 114 236 Cuts at: 8281 8402 8459 8474 8987 9057 9197 9265 9458 Size: 121 57 15 513 70 140 68 193 Cuts at: 9458 9510 9584 9657 9671 9767 9930 9950 9978 Size: 52 74 73 14 96 163 20 28 Cuts at: 9978 10036 10258 10436 10806 11008 11071 11264 11519 Size: 58 222 178 370 202 63 193 255 Cuts at: 11519 11897 11992 12835 13199 13220 13375 13624 13641 Size: 378 95 843 364 21 155 249 17 Fragments arranged by size: 984 963 843 680 666 652 582 513 496 444 378 370 364 352 299 255 254 249 240 239 236 222 202 193 193 178 163 162 155 155 140 137 121 114 96 95 89 80 74 73 70 68 63 63 58 57 57 53 52 48 46 38 37 30 29 28 28 21 21 20 17 15 14 7 BspEI T'CCGG_A Cuts at: 0 7217 13641 Size: 7217 6424 BspHI T'CATG_A Cuts at: 0 9273 13641 Size: 9273 4368 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 905 1580 1589 1649 1802 2294 2439 3216 Size: 905 675 9 60 153 492 145 777 Cuts at: 3216 3962 4494 4674 5754 6318 6537 6617 6813 Size: 746 532 180 1080 564 219 80 196 Cuts at: 6813 7845 8081 8600 8828 9149 10656 11000 11096 Size: 1032 236 519 228 321 1507 344 96 Cuts at: 11096 11691 12311 13315 13641 Size: 595 620 1004 326 Fragments arranged by size: 1507 1080 1032 1004 905 777 746 675 620 595 564 532 519 492 344 326 321 236 228 219 196 180 153 145 96 80 60 9 BsrBI CCG'CTC Cuts at: 0 117 681 1045 1137 1719 2720 3507 3537 Size: 117 564 364 92 582 1001 787 30 Cuts at: 3537 4080 4161 4647 4833 4863 4884 5496 5721 Size: 543 81 486 186 30 21 612 225 Cuts at: 5721 5940 6036 6082 6345 7113 9633 10584 11220 Size: 219 96 46 263 768 2520 951 636 Cuts at: 11220 11295 12381 12593 12647 12731 13641 Size: 75 1086 212 54 84 910 Fragments arranged by size: 2520 1086 1001 951 910 787 768 636 612 582 564 543 486 364 263 225 219 212 186 117 96 92 84 81 75 54 46 30 30 21 BsrFI r'CCGG_y Cuts at: 0 593 632 2039 2849 3042 3354 3882 4337 Size: 593 39 1407 810 193 312 528 455 Cuts at: 4337 5377 5381 7981 8234 8489 8909 9184 9564 Size: 1040 4 2600 253 255 420 275 380 Cuts at: 9564 9596 10072 10300 10828 11305 11416 11608 11917 Size: 32 476 228 528 477 111 192 309 Cuts at: 11917 12176 12670 12718 12976 13147 13180 13471 13483 Size: 259 494 48 258 171 33 291 12 Cuts at: 13483 13495 13525 13641 Size: 12 30 116 Fragments arranged by size: 2600 1407 1040 810 593 528 528 494 477 476 455 420 380 312 309 291 275 259 258 255 253 228 193 192 171 116 111 48 39 33 32 30 12 12 4 BsrGI T'GTAC_A Cuts at: 0 10670 13641 Size: 10670 2971 BssHII G'CGCG_C Cuts at: 0 4582 4867 8313 12024 13641 Size: 4582 285 3446 3711 1617 Fragments arranged by size: 4582 3711 3446 1617 285 BssSI C'ACGA_G Cuts at: 0 1432 3322 8575 10228 10267 11254 13641 Size: 1432 1890 5253 1653 39 987 2387 Fragments arranged by size: 5253 2387 1890 1653 1432 987 39 BstAPI GCAn_nnn'nTGC Cuts at: 0 1530 1581 2220 2655 2904 3018 3570 3759 Size: 1530 51 639 435 249 114 552 189 Cuts at: 3759 4188 4272 4431 4435 6204 6222 6531 6954 Size: 429 84 159 4 1769 18 309 423 Cuts at: 6954 7434 7452 7470 9247 9390 10064 11798 12930 Size: 480 18 18 1777 143 674 1734 1132 Cuts at: 12930 13641 Size: 711 Fragments arranged by size: 1777 1769 1734 1530 1132 711 674 639 552 480 435 429 423 309 249 189 159 143 114 84 51 18 18 18 4 BstEII G'GTnAC_C Cuts at: 0 7281 8763 9834 10836 11301 11760 13641 Size: 7281 1482 1071 1002 465 459 1881 Fragments arranged by size: 7281 1881 1482 1071 1002 465 459 BstXI CCAn_nnnn'nTGG Cuts at: 0 454 2032 3252 3276 3711 13641 Size: 454 1578 1220 24 435 9930 Fragments arranged by size: 9930 1578 1220 454 435 24 BstYI r'GATC_y Cuts at: 0 434 944 3695 4295 4358 5777 7304 8354 Size: 434 510 2751 600 63 1419 1527 1050 Cuts at: 8354 12233 12751 13328 13641 Size: 3879 518 577 313 Fragments arranged by size: 3879 2751 1527 1419 1050 600 577 518 510 434 313 63 Bsu36I CC'TnA_GG Cuts at: 0 713 1834 8205 12164 13641 Size: 713 1121 6371 3959 1477 Fragments arranged by size: 6371 3959 1477 1121 713 BtgI C'CryG_G Cuts at: 0 2072 3285 3780 5129 6647 7907 8030 8432 Size: 2072 1213 495 1349 1518 1260 123 402 Cuts at: 8432 8474 9161 9230 9416 9725 9839 10409 10737 Size: 42 687 69 186 309 114 570 328 Cuts at: 10737 11072 11655 11678 11717 12111 12143 12185 12836 Size: 335 583 23 39 394 32 42 651 Cuts at: 12836 13506 13634 13641 Size: 670 128 7 Fragments arranged by size: 2072 1518 1349 1260 1213 687 670 651 583 570 495 402 394 335 328 309 186 128 123 114 69 42 42 39 32 23 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 227 357 549 3051 3152 7338 9060 11787 Size: 227 130 192 2502 101 4186 1722 2727 Cuts at: 11787 12609 13641 Size: 822 1032 Fragments arranged by size: 4186 2727 2502 1722 1032 822 227 192 130 101 BtsI GCAGTG_nn' Cuts at: 0 2113 2230 4429 4722 6568 7844 9248 9785 Size: 2113 117 2199 293 1846 1276 1404 537 Cuts at: 9785 10065 13641 Size: 280 3576 Fragments arranged by size: 3576 2199 2113 1846 1404 1276 537 293 280 117 ClaI AT'CG_AT Cuts at: 0 12614 13641 Size: 12614 1027 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1487 1522 12702 12737 13641 Size: 1487 35 11180 35 904 Fragments arranged by size: 11180 1487 904 35 35 DraIII CAC_nnn'GTG Cuts at: 0 149 1614 1729 2645 4435 8469 9453 10446 Size: 149 1465 115 916 1790 4034 984 993 Cuts at: 10446 13641 Size: 3195 Fragments arranged by size: 4034 3195 1790 1465 993 984 916 149 115 DrdI GACnn_nn'nnGTC Cuts at: 0 724 12565 13101 13641 Size: 724 11841 536 540 Fragments arranged by size: 11841 724 540 536 EaeI y'GGCC_r Cuts at: 0 216 476 567 896 1124 2351 2429 2732 Size: 216 260 91 329 228 1227 78 303 Cuts at: 2732 3356 3713 3896 4085 4124 5126 5270 5303 Size: 624 357 183 189 39 1002 144 33 Cuts at: 5303 5379 5396 5456 5516 5810 5984 7166 7571 Size: 76 17 60 60 294 174 1182 405 Cuts at: 7571 7646 7884 8306 8346 9158 9566 10178 10830 Size: 75 238 422 40 812 408 612 652 Cuts at: 10830 11411 11610 11912 12017 13106 13149 13581 13641 Size: 581 199 302 105 1089 43 432 60 Fragments arranged by size: 1227 1182 1089 1002 812 652 624 612 581 432 422 408 405 357 329 303 302 294 260 238 228 216 199 189 183 174 144 105 91 78 76 75 60 60 60 43 40 39 33 17 EagI C'GGCC_G Cuts at: 0 567 896 5379 5810 5984 8306 10830 12017 Size: 567 329 4483 431 174 2322 2524 1187 Cuts at: 12017 13106 13581 13641 Size: 1089 475 60 Fragments arranged by size: 4483 2524 2322 1187 1089 567 475 431 329 174 60 EarI CTCTTCn'nnn_ Cuts at: 0 580 1360 2665 2927 4957 5426 5968 6143 Size: 580 780 1305 262 2030 469 542 175 Cuts at: 6143 6329 6694 7561 8368 10345 10697 11350 12079 Size: 186 365 867 807 1977 352 653 729 Cuts at: 12079 12217 13557 13641 Size: 138 1340 84 Fragments arranged by size: 2030 1977 1340 1305 867 807 780 729 653 580 542 469 365 352 262 186 175 138 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 691 1348 2250 4171 4306 4459 4468 4552 Size: 691 657 902 1921 135 153 9 84 Cuts at: 4552 4648 4657 4741 4957 5020 5050 5116 5443 Size: 96 9 84 216 63 30 66 327 Cuts at: 5443 5506 5569 5596 5623 5839 5944 6160 6262 Size: 63 63 27 27 216 105 216 102 Cuts at: 6262 6292 6313 6469 6655 6844 7000 7087 7243 Size: 30 21 156 186 189 156 87 156 Cuts at: 7243 7965 8398 8545 10375 10522 10651 10885 11031 Size: 722 433 147 1830 147 129 234 146 Cuts at: 11031 11050 11863 12109 13641 Size: 19 813 246 1532 Fragments arranged by size: 1921 1830 1532 902 813 722 691 657 433 327 246 234 216 216 216 189 186 156 156 156 153 147 147 146 135 129 105 102 96 87 84 84 66 63 63 63 30 30 27 27 21 19 9 9 EcoICRI GAG'CTC Cuts at: 0 2022 2964 3372 3603 4283 6687 7263 8043 Size: 2022 942 408 231 680 2404 576 780 Cuts at: 8043 8985 9582 9669 9765 9948 11895 13641 Size: 942 597 87 96 183 1947 1746 Fragments arranged by size: 2404 2022 1947 1746 942 942 780 680 597 576 408 231 183 96 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 537 579 667 859 909 985 1057 Size: 259 278 42 88 192 50 76 72 Cuts at: 1057 1153 1165 1192 1495 1570 1864 1950 2014 Size: 96 12 27 303 75 294 86 64 Cuts at: 2014 2020 2173 2299 2401 2635 2770 2806 3007 Size: 6 153 126 102 234 135 36 201 Cuts at: 3007 3220 3241 3274 3415 3784 3835 4126 4633 Size: 213 21 33 141 369 51 291 507 Cuts at: 4633 4720 4795 4912 5101 5215 5386 5659 5737 Size: 87 75 117 189 114 171 273 78 Cuts at: 5737 5809 5851 5857 5920 6043 6370 6604 6655 Size: 72 42 6 63 123 327 234 51 Cuts at: 6655 7123 7147 7321 7384 7567 7747 7822 8128 Size: 468 24 174 63 183 180 75 306 Cuts at: 8128 8209 8437 8623 8980 9112 9151 9379 9568 Size: 81 228 186 357 132 39 228 189 Cuts at: 9568 9897 10105 10186 10414 10464 10600 11191 11479 Size: 329 208 81 228 50 136 591 288 Cuts at: 11479 11920 11968 12124 12148 12382 12526 12771 12886 Size: 441 48 156 24 234 144 245 115 Cuts at: 12886 12955 13141 13183 13411 13641 Size: 69 186 42 228 230 Fragments arranged by size: 591 507 468 441 369 357 329 327 306 303 294 291 288 278 273 259 245 234 234 234 230 228 228 228 228 213 208 201 192 189 189 186 186 183 180 174 171 156 153 144 141 136 135 132 126 123 117 115 114 102 96 88 87 86 81 81 78 76 75 75 75 72 72 69 64 63 63 51 51 50 50 48 42 42 42 39 36 33 27 24 24 21 12 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 169 238 658 2290 2917 3763 4657 4789 Size: 169 69 420 1632 627 846 894 132 Cuts at: 4789 4972 6799 7345 8188 8416 9349 9361 9490 Size: 183 1827 546 843 228 933 12 129 Cuts at: 9490 9895 10165 10342 10393 10762 10957 11020 11488 Size: 405 270 177 51 369 195 63 468 Cuts at: 11488 12076 12127 13123 13225 13641 Size: 588 51 996 102 416 Fragments arranged by size: 1827 1632 996 933 894 846 843 627 588 546 468 420 416 405 369 270 228 195 183 177 169 132 129 102 69 63 51 51 12 EcoO109I rG'GnC_Cy Cuts at: 0 36 92 243 528 684 1578 1838 2283 Size: 36 56 151 285 156 894 260 445 Cuts at: 2283 2494 2631 3317 3756 4599 4608 6897 7467 Size: 211 137 686 439 843 9 2289 570 Cuts at: 7467 8278 8334 8382 8619 8817 8925 9262 9318 Size: 811 56 48 237 198 108 337 56 Cuts at: 9318 9354 9366 9387 9506 9507 9720 9963 10017 Size: 36 12 21 119 1 213 243 54 Cuts at: 10017 10131 10255 10359 10596 10950 11250 11260 11385 Size: 114 124 104 237 354 300 10 125 Cuts at: 11385 11739 11989 12045 12093 12480 12791 12832 13014 Size: 354 250 56 48 387 311 41 182 Cuts at: 13014 13080 13116 13230 13601 13620 13621 13641 Size: 66 36 114 371 19 1 20 Fragments arranged by size: 2289 894 843 811 686 570 445 439 387 371 354 354 337 311 300 285 260 250 243 237 237 213 211 198 182 156 151 137 125 124 119 114 114 108 104 66 56 56 56 56 54 48 48 41 36 36 36 21 20 19 12 10 9 1 1 EcoRI G'AATT_C Cuts at: 0 1699 13641 Size: 1699 11942 EcoRV GAT'ATC Cuts at: 0 444 13641 Size: 444 13197 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 156 5814 8574 10551 11667 12285 13641 Size: 156 5658 2760 1977 1116 618 1356 Fragments arranged by size: 5658 2760 1977 1356 1116 618 156 FseI GG_CCGG'CC Cuts at: 0 5381 10832 13151 13641 Size: 5381 5451 2319 490 Fragments arranged by size: 5451 5381 2319 490 FspI TGC'GCA Cuts at: 0 4345 5695 5878 6415 7016 8932 11530 12409 Size: 4345 1350 183 537 601 1916 2598 879 Cuts at: 12409 13641 Size: 1232 Fragments arranged by size: 4345 2598 1916 1350 1232 879 601 537 183 HaeII r_GCGC'y Cuts at: 0 1440 1542 1625 1981 2769 2952 3528 3561 Size: 1440 102 83 356 788 183 576 33 Cuts at: 3561 3662 3780 4398 4494 4653 4782 4899 4924 Size: 101 118 618 96 159 129 117 25 Cuts at: 4924 5124 5595 5682 5788 5949 6022 6069 6792 Size: 200 471 87 106 161 73 47 723 Cuts at: 6792 7139 7152 7215 7341 7521 8610 9015 9221 Size: 347 13 63 126 180 1089 405 206 Cuts at: 9221 10218 11562 11725 12417 12603 12987 13158 13289 Size: 997 1344 163 692 186 384 171 131 Cuts at: 13289 13641 Size: 352 Fragments arranged by size: 1440 1344 1089 997 788 723 692 618 576 471 405 384 356 352 347 206 200 186 183 180 171 163 161 159 131 129 126 118 117 106 102 101 96 87 83 73 63 47 33 25 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 730 762 5314 5346 6076 6108 9892 9924 Size: 730 32 4552 32 730 32 3784 32 Cuts at: 9924 10090 10122 10303 10335 10959 10991 11023 11055 Size: 166 32 181 32 624 32 32 32 Cuts at: 11055 11494 11526 12037 12069 12410 12442 12577 12609 Size: 439 32 511 32 341 32 135 32 Cuts at: 12609 12691 12723 12859 12891 13641 Size: 82 32 136 32 750 Fragments arranged by size: 4552 3784 750 730 730 624 511 439 341 181 166 136 135 82 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 156 8250 13008 13641 Size: 156 8094 4758 633 Fragments arranged by size: 8094 4758 633 156 HindIII A'AGCT_T Cuts at: 0 7479 13641 Size: 7479 6162 KasI G'GCGC_C Cuts at: 0 1538 3658 4649 5945 6065 12599 13285 13641 Size: 1538 2120 991 1296 120 6534 686 356 Fragments arranged by size: 6534 2120 1538 1296 991 686 356 120 KpnI G_GTAC'C Cuts at: 0 525 13641 Size: 525 13116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 443 1241 4361 7410 9152 9176 9380 10757 Size: 443 798 3120 3049 1742 24 204 1377 Cuts at: 10757 11612 13641 Size: 855 2029 Fragments arranged by size: 3120 3049 2029 1742 1377 855 798 443 204 24 MscI TGG'CCA Cuts at: 0 218 1126 2353 2734 5272 5305 7573 7648 Size: 218 908 1227 381 2538 33 2268 75 Cuts at: 7648 8348 11413 13641 Size: 700 3065 2228 Fragments arranged by size: 3065 2538 2268 2228 1227 908 700 381 218 75 33 MslI CAynn'nnrTG Cuts at: 0 188 616 1948 2471 3051 8032 8881 8884 Size: 188 428 1332 523 580 4981 849 3 Cuts at: 8884 9160 10666 13641 Size: 276 1506 2975 Fragments arranged by size: 4981 2975 1506 1332 849 580 523 428 276 188 3 MspA1I CmG'CkG Cuts at: 0 557 834 1089 1233 1299 1662 2127 2319 Size: 557 277 255 144 66 363 465 192 Cuts at: 2319 2379 2487 2901 2985 3030 3287 3495 3549 Size: 60 108 414 84 45 257 208 54 Cuts at: 3549 3624 3711 3741 3855 4062 4116 4122 4272 Size: 75 87 30 114 207 54 6 150 Cuts at: 4272 4596 4773 4815 5058 5091 5097 5131 5184 Size: 324 177 42 243 33 6 34 53 Cuts at: 5184 5190 5448 5454 5481 5628 5691 5757 5815 Size: 6 258 6 27 147 63 66 58 Cuts at: 5815 5916 5922 6009 6105 6111 6234 6487 6582 Size: 101 6 87 96 6 123 253 95 Cuts at: 6582 6630 6885 6912 7068 7095 7119 7125 7449 Size: 48 255 27 156 27 24 6 324 Cuts at: 7449 7512 7587 7605 7638 7725 7737 7791 7909 Size: 63 75 18 33 87 12 54 118 Cuts at: 7909 8010 8157 8811 8997 9411 9591 9609 9798 Size: 101 147 654 186 414 180 18 189 Cuts at: 9798 9917 10275 10575 10800 10809 11409 11508 11577 Size: 119 358 300 225 9 600 99 69 Cuts at: 11577 11616 11730 11798 12309 12360 12798 12927 13442 Size: 39 114 68 511 51 438 129 515 Cuts at: 13442 13459 13508 13641 Size: 17 49 133 Fragments arranged by size: 654 600 557 515 511 465 438 414 414 363 358 324 324 300 277 258 257 255 255 253 243 225 208 207 192 189 186 180 177 156 150 147 147 144 133 129 123 119 118 114 114 108 101 101 99 96 95 87 87 87 84 75 75 69 68 66 66 63 63 60 58 54 54 54 53 51 49 48 45 42 39 34 33 33 30 27 27 27 24 18 18 17 12 9 6 6 6 6 6 6 6 NaeI GCC'GGC Cuts at: 0 3044 3356 4339 5379 7983 8911 9186 9566 Size: 3044 312 983 1040 2604 928 275 380 Cuts at: 9566 9598 10074 10830 12672 13149 13497 13641 Size: 32 476 756 1842 477 348 144 Fragments arranged by size: 3044 2604 1842 1040 983 928 756 477 476 380 348 312 275 144 32 NarI GG'CG_CC Cuts at: 0 1539 3659 4650 5946 6066 12600 13286 13641 Size: 1539 2120 991 1296 120 6534 686 355 Fragments arranged by size: 6534 2120 1539 1296 991 686 355 120 NcoI C'CATG_G Cuts at: 0 11678 12111 13641 Size: 11678 433 1530 Fragments arranged by size: 11678 1530 433 NgoMIV G'CCGG_C Cuts at: 0 3042 3354 4337 5377 7981 8909 9184 9564 Size: 3042 312 983 1040 2604 928 275 380 Cuts at: 9564 9596 10072 10828 12670 13147 13495 13641 Size: 32 476 756 1842 477 348 146 Fragments arranged by size: 3042 2604 1842 1040 983 928 756 477 476 380 348 312 275 146 32 NotI GC'GGCC_GC Cuts at: 0 5810 13641 Size: 5810 7831 NspI r_CATG'y Cuts at: 0 4191 12705 13641 Size: 4191 8514 936 Fragments arranged by size: 8514 4191 936 PasI CC'CwG_GG Cuts at: 0 13614 13641 Size: 13614 27 PflMI CCAn_nnn'nTGG Cuts at: 0 1035 1089 2490 2634 10812 13641 Size: 1035 54 1401 144 8178 2829 Fragments arranged by size: 8178 2829 1401 1035 144 54 PfoI T'CCnGG_A Cuts at: 0 704 1130 3365 3938 7559 7799 8393 10997 Size: 704 426 2235 573 3621 240 594 2604 Cuts at: 10997 11783 11897 12787 12848 13160 13641 Size: 786 114 890 61 312 481 Fragments arranged by size: 3621 2604 2235 890 786 704 594 573 481 426 312 240 114 61 PmlI CAC'GTG Cuts at: 0 1122 2661 4800 13641 Size: 1122 1539 2139 8841 Fragments arranged by size: 8841 2139 1539 1122 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 10939 10971 13641 Size: 10939 32 2670 Fragments arranged by size: 10939 2670 32 PpuMI rG'GwC_Cy Cuts at: 0 243 684 1578 1838 2283 6897 8334 8619 Size: 243 441 894 260 445 4614 1437 285 Cuts at: 8619 8817 9318 9720 9963 10017 10596 10950 12045 Size: 198 501 402 243 54 579 354 1095 Cuts at: 12045 12791 13080 13601 13641 Size: 746 289 521 40 Fragments arranged by size: 4614 1437 1095 894 746 579 521 501 445 441 402 354 289 285 260 243 243 198 54 40 PshAI GACnn'nnGTC Cuts at: 0 10009 13641 Size: 10009 3632 PspOMI G'GGCC_C Cuts at: 0 2493 3317 3407 4598 8277 9261 9506 9653 Size: 2493 824 90 1191 3679 984 245 147 Cuts at: 9653 10254 10802 11260 11988 12831 13620 13641 Size: 601 548 458 728 843 789 21 Fragments arranged by size: 3679 2493 1191 984 843 824 789 728 601 548 458 245 147 90 21 PspXI vC'TCGA_Gb Cuts at: 0 3373 3604 13641 Size: 3373 231 10037 Fragments arranged by size: 10037 3373 231 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 613 645 2152 2184 3988 4020 8572 8604 Size: 613 32 1507 32 1804 32 4552 32 Cuts at: 8604 10549 10581 12283 12315 13641 Size: 1945 32 1702 32 1326 Fragments arranged by size: 4552 1945 1804 1702 1507 1326 613 32 32 32 32 32 32 PstI C_TGCA'G Cuts at: 0 302 833 1205 1259 1284 1526 1577 1799 Size: 302 531 372 54 25 242 51 222 Cuts at: 1799 2102 2225 2294 2884 2909 2984 3491 3671 Size: 303 123 69 590 25 75 507 180 Cuts at: 3671 3746 4181 4268 4379 4427 4607 4631 4736 Size: 75 435 87 111 48 180 24 105 Cuts at: 4736 4790 4940 5219 5235 6110 6143 6209 6405 Size: 54 150 279 16 875 33 66 196 Cuts at: 6405 6581 6629 6800 6998 7040 7409 7457 7475 Size: 176 48 171 198 42 369 48 18 Cuts at: 7475 7721 7796 7976 8009 8078 8150 8597 9002 Size: 246 75 180 33 69 72 447 405 Cuts at: 9002 9686 10055 11729 12308 13238 13641 Size: 684 369 1674 579 930 403 Fragments arranged by size: 1674 930 875 684 590 579 531 507 447 435 405 403 372 369 369 303 302 279 246 242 222 198 196 180 180 180 176 171 150 123 111 105 87 75 75 75 72 69 69 66 54 54 51 48 48 48 42 33 33 25 25 24 18 16 PvuII CAG'CTG Cuts at: 0 557 834 1233 1662 2127 2319 2379 2487 Size: 557 277 399 429 465 192 60 108 Cuts at: 2487 2901 2985 3549 3624 3741 3855 4122 4272 Size: 414 84 564 75 117 114 267 150 Cuts at: 4272 4773 4815 5097 5190 5454 5691 5922 6105 Size: 501 42 282 93 264 237 231 183 Cuts at: 6105 6111 6234 6487 6582 6885 7068 7125 7449 Size: 6 123 253 95 303 183 57 324 Cuts at: 7449 7587 7725 7791 8157 8997 9411 9609 10275 Size: 138 138 66 366 840 414 198 666 Cuts at: 10275 10575 10809 11409 11508 11577 11616 11730 11798 Size: 300 234 600 99 69 39 114 68 Cuts at: 11798 12309 12798 13641 Size: 511 489 843 Fragments arranged by size: 843 840 666 600 564 557 511 501 489 465 429 414 414 399 366 324 303 300 282 277 267 264 253 237 234 231 198 192 183 183 150 138 138 123 117 114 114 108 99 95 93 84 75 69 68 66 60 57 42 39 6 SacI G_AGCT'C Cuts at: 0 2024 2966 3374 3605 4285 6689 7265 8045 Size: 2024 942 408 231 680 2404 576 780 Cuts at: 8045 8987 9584 9671 9767 9950 11897 13641 Size: 942 597 87 96 183 1947 1744 Fragments arranged by size: 2404 2024 1947 1744 942 942 780 680 597 576 408 231 183 96 87 SacII CC_GC'GG Cuts at: 0 3288 5132 7910 13509 13641 Size: 3288 1844 2778 5599 132 Fragments arranged by size: 5599 3288 2778 1844 132 SanDI GG'GwC_CC Cuts at: 0 9318 13641 Size: 9318 4323 SapI GCTCTTCn'nnn_ Cuts at: 0 1360 2665 4957 5968 6694 7561 11350 13641 Size: 1360 1305 2292 1011 726 867 3789 2291 Fragments arranged by size: 3789 2292 2291 1360 1305 1011 867 726 SbfI CC_TGCA'GG Cuts at: 0 1577 4607 4790 6800 8078 13641 Size: 1577 3030 183 2010 1278 5563 Fragments arranged by size: 5563 3030 2010 1577 1278 183 ScaI AGT'ACT Cuts at: 0 9781 13641 Size: 9781 3860 SexAI A'CCwGG_T Cuts at: 0 12902 13641 Size: 12902 739 SfcI C'TryA_G Cuts at: 0 298 829 1201 1255 1280 1522 1573 1795 Size: 298 531 372 54 25 242 51 222 Cuts at: 1795 2098 2122 2221 2290 2880 2905 2980 3487 Size: 303 24 99 69 590 25 75 507 Cuts at: 3487 3667 3742 3966 4177 4264 4375 4423 4603 Size: 180 75 224 211 87 111 48 180 Cuts at: 4603 4627 4732 4786 4936 5215 5231 6106 6139 Size: 24 105 54 150 279 16 875 33 Cuts at: 6139 6193 6205 6401 6577 6625 6796 6994 7036 Size: 54 12 196 176 48 171 198 42 Cuts at: 7036 7405 7453 7471 7507 7717 7792 7972 8005 Size: 369 48 18 36 210 75 180 33 Cuts at: 8005 8074 8146 8593 8998 9540 9682 10051 10305 Size: 69 72 447 405 542 142 369 254 Cuts at: 10305 11725 12304 13234 13422 13431 13641 Size: 1420 579 930 188 9 210 Fragments arranged by size: 1420 930 875 590 579 542 531 507 447 405 372 369 369 303 298 279 254 242 224 222 211 210 210 198 196 188 180 180 180 176 171 150 142 111 105 99 87 75 75 75 72 69 69 54 54 54 51 48 48 48 42 36 33 33 25 25 24 24 18 16 12 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2359 2599 3355 3385 3895 4606 5404 6121 Size: 2359 240 756 30 510 711 798 717 Cuts at: 6121 6652 9260 13060 13114 13641 Size: 531 2608 3800 54 527 Fragments arranged by size: 3800 2608 2359 798 756 717 711 531 527 510 240 54 30 SfoI GGC'GCC Cuts at: 0 1540 3660 4651 5947 6067 12601 13287 13641 Size: 1540 2120 991 1296 120 6534 686 354 Fragments arranged by size: 6534 2120 1540 1296 991 686 354 120 SgrAI Cr'CCGG_yG Cuts at: 0 11416 12718 12976 13641 Size: 11416 1302 258 665 Fragments arranged by size: 11416 1302 665 258 SmaI CCC'GGG Cuts at: 0 268 1759 6289 6382 8704 9379 13492 13641 Size: 268 1491 4530 93 2322 675 4113 149 Fragments arranged by size: 4530 4113 2322 1491 675 268 149 93 SmlI C'TyrA_G Cuts at: 0 1063 1375 3262 3334 3373 3553 3604 3877 Size: 1063 312 1887 72 39 180 51 273 Cuts at: 3877 4906 5542 5566 6727 6934 6973 7153 7411 Size: 1029 636 24 1161 207 39 180 258 Cuts at: 7411 9991 10978 11029 11128 13324 13641 Size: 2580 987 51 99 2196 317 Fragments arranged by size: 2580 2196 1887 1161 1063 1029 987 636 317 312 273 258 207 180 180 99 72 51 51 39 39 24 SphI G_CATG'C Cuts at: 0 4191 13641 Size: 4191 9450 SrfI GCCC'GGGC Cuts at: 0 6382 13641 Size: 6382 7259 StuI AGG'CCT Cuts at: 0 664 1679 2348 2803 2992 3388 5620 9538 Size: 664 1015 669 455 189 396 2232 3918 Cuts at: 9538 12469 12946 13063 13641 Size: 2931 477 117 578 Fragments arranged by size: 3918 2931 2232 1015 669 664 578 477 455 396 189 117 StyI C'CwwG_G Cuts at: 0 399 479 992 1016 1823 2363 3383 3653 Size: 399 80 513 24 807 540 1020 270 Cuts at: 3653 3908 4304 5273 6047 6917 7574 8549 8666 Size: 255 396 969 774 870 657 975 117 Cuts at: 8666 9520 9533 10094 10526 10841 11678 11828 12111 Size: 854 13 561 432 315 837 150 283 Cuts at: 12111 12260 13412 13641 Size: 149 1152 229 Fragments arranged by size: 1152 1020 975 969 870 854 837 807 774 657 561 540 513 432 399 396 315 283 270 255 229 150 149 117 80 24 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1581 2408 3598 6607 7285 9718 12256 13641 Size: 1581 827 1190 3009 678 2433 2538 1385 Fragments arranged by size: 3009 2538 2433 1581 1385 1190 827 678 TatI w'GTAC_w Cuts at: 0 1460 2327 3821 3995 9779 10670 13641 Size: 1460 867 1494 174 5784 891 2971 Fragments arranged by size: 5784 2971 1494 1460 891 867 174 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1090 1122 1873 1905 7289 7321 10729 10761 Size: 1090 32 751 32 5384 32 3408 32 Cuts at: 10761 11004 11036 12514 12546 13641 Size: 243 32 1478 32 1095 Fragments arranged by size: 5384 3408 1478 1095 1090 751 243 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 13003 13641 Size: 13003 638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1203 5361 7359 9948 10188 11685 12795 13641 Size: 1203 4158 1998 2589 240 1497 1110 846 Fragments arranged by size: 4158 2589 1998 1497 1203 1110 846 240 XhoI C'TCGA_G Cuts at: 0 3373 3604 10978 11029 13641 Size: 3373 231 7374 51 2612 Fragments arranged by size: 7374 3373 2612 231 51 XmaI C'CCGG_G Cuts at: 0 266 1757 6287 6380 8702 9377 13490 13641 Size: 266 1491 4530 93 2322 675 4113 151 Fragments arranged by size: 4530 4113 2322 1491 675 266 151 93 XmnI GAAnn'nnTTC Cuts at: 0 139 13063 13641 Size: 139 12924 578 Fragments arranged by size: 12924 578 139 ZraI GAC'GTC Cuts at: 0 8709 12389 13641 Size: 8709 3680 1252 Fragments arranged by size: 8709 3680 1252 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AflII AscI AseI AsiSI AvrII BaeI BciVI BfrBI BmtI BsrDI BstBI BstZ17I DraI FspAI HpaI MfeI MluI NdeI NheI NruI NsiI PacI PciI PmeI PsiI PvuI RsrII SalI SgfI SnaBI SpeI SspI SwaI XbaI