(Circular) (Six-base) MAPSORT of: pFN21AE2499.seq Check: 2098 from: 1 to: 18427 pFN21AE2499 18427 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4525 6580 7840 8404 8623 8899 9931 12742 15401 Size: 2055 1260 564 219 276 1032 2811 2659 Cuts at: 15401 4525 Size: 7551 Fragments arranged by size: 7551 2811 2659 2055 1260 1032 564 276 219 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10797 14477 16237 278 Size: 53 83 186 936 9261 3680 1760 2468 Fragments arranged by size: 9261 3680 2468 1760 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 10770 13170 15770 1937 Size: 8833 2400 2600 4594 Fragments arranged by size: 8833 4594 2600 2400 Acc65I G'GTAC_C Cuts at: 2607 15748 2607 Size: 13141 5286 AclI AA'CG_TT Cuts at: 1563 16554 16927 1563 Size: 14991 373 3063 Fragments arranged by size: 14991 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2061 2945 3071 3251 3581 4106 4259 4487 Size: 429 884 126 180 330 525 153 228 Cuts at: 4487 4856 5501 7895 8456 8741 10214 11237 11465 Size: 369 645 2394 561 285 1473 1023 228 Cuts at: 11465 11983 12550 14054 14210 14468 15227 16490 17874 Size: 518 567 1504 156 258 759 1263 1384 Cuts at: 17874 18142 1632 Size: 268 1917 Fragments arranged by size: 2394 1917 1504 1473 1384 1263 1023 884 759 645 567 561 525 518 429 369 330 285 268 258 228 228 180 156 153 126 AfeI AGC'GCT Cuts at: 5036 5612 5645 6578 6866 6983 8876 9223 9299 Size: 576 33 933 288 117 1893 347 76 Cuts at: 9299 9605 10694 11099 15071 15242 5036 Size: 306 1089 405 3972 171 8221 Fragments arranged by size: 8221 3972 1893 1089 933 576 405 347 306 288 171 117 76 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 18230 AflIII A'CryG_T Cuts at: 1203 6883 17326 18303 1203 Size: 5680 10443 977 1327 Fragments arranged by size: 10443 5680 1327 977 AgeI A'CCGG_T Cuts at: 14003 15062 17258 14003 Size: 1059 2196 15172 Fragments arranged by size: 15172 2196 1059 AhdI GACnn_n'nnGTC Cuts at: 2830 13594 17156 2830 Size: 10764 3562 4101 Fragments arranged by size: 10764 4101 3562 AleI CACnn'nnGTG Cuts at: 1166 2274 4557 5137 10118 10970 12752 1166 Size: 1108 2283 580 4981 852 1782 6841 Fragments arranged by size: 6841 4981 2283 1782 1108 852 580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6443 6819 9963 10514 11042 12491 14225 15482 Size: 5259 376 3144 551 528 1449 1734 1257 Cuts at: 15482 1184 Size: 4129 Fragments arranged by size: 5259 4129 3144 1734 1449 1257 551 528 376 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 2988 3019 3020 2987 Size: 1 31 1 18394 Fragments arranged by size: 18394 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2039 2617 3049 3667 4306 4720 4987 4990 4999 Size: 578 432 618 639 414 267 3 9 Cuts at: 4999 5845 6358 6517 6697 7210 7279 7342 7450 Size: 846 513 159 180 513 69 63 108 Cuts at: 7450 8290 8617 8647 8719 9154 9385 9538 9556 Size: 840 327 30 72 435 231 153 18 Cuts at: 9556 9673 9727 9811 9865 9886 10057 10246 10687 Size: 117 54 84 54 21 171 189 441 Cuts at: 10687 11083 11197 11443 11476 11893 12150 12304 12364 Size: 396 114 246 33 417 257 154 60 Cuts at: 12364 12664 12994 13195 15016 15205 15262 15358 16170 Size: 300 330 201 1821 189 57 96 812 Cuts at: 16170 17742 2039 Size: 1572 2724 Fragments arranged by size: 2724 1821 1572 846 840 812 639 618 578 513 513 441 435 432 417 414 396 330 327 300 267 257 246 231 201 189 189 180 171 159 154 153 117 114 108 96 84 72 69 63 60 57 54 54 33 30 21 18 9 3 ApaI G_GGCC'C Cuts at: 4583 5407 5497 6688 10367 11351 11596 11743 12344 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 12344 12892 13350 14078 14921 15710 4583 Size: 548 458 728 843 789 7300 Fragments arranged by size: 7300 3679 1191 984 843 824 789 728 601 548 458 245 147 90 ApaLI G'TGCA_C Cuts at: 4550 5010 5096 6522 10541 12518 16484 17640 4550 Size: 460 86 1426 4019 1977 3966 1156 5337 Fragments arranged by size: 5337 4019 3966 1977 1426 1156 460 86 ApoI r'AATT_y Cuts at: 1493 3785 8600 15736 15962 16114 18223 1493 Size: 2292 4815 7136 226 152 2109 1697 Fragments arranged by size: 7136 4815 2292 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 16981 160 Size: 872 15949 1606 Fragments arranged by size: 15949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 18427 AvaI C'yCGr_G Cuts at: 1870 1943 2335 2352 3629 3843 4928 5459 5690 Size: 73 392 17 1277 214 1085 531 231 Cuts at: 5690 8373 8466 10367 10709 10788 11351 11463 11562 Size: 2683 93 1901 342 79 563 112 99 Cuts at: 11562 11736 12344 12406 12686 12875 13064 13115 13349 Size: 174 608 62 280 189 189 51 234 Cuts at: 13349 14078 14522 14794 15576 15658 15682 15752 1870 Size: 729 444 272 782 82 24 70 4545 Fragments arranged by size: 4545 2683 1901 1277 1085 782 729 608 563 531 444 392 342 280 272 234 231 214 189 189 174 112 99 93 82 79 73 70 62 51 24 17 BamHI G'GATC_C Cuts at: 14837 15757 16124 14837 Size: 920 367 17140 Fragments arranged by size: 17140 920 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2418 2607 3154 3474 3624 Size: 325 217 627 631 189 547 320 150 Cuts at: 3624 3848 4657 4734 5744 6735 7193 8031 8151 Size: 224 809 77 1010 991 458 838 120 Cuts at: 8151 11026 11150 11273 11602 11679 13091 13674 13691 Size: 2875 124 123 329 77 1412 583 17 Cuts at: 13691 13877 14685 15371 15748 17203 618 Size: 186 808 686 377 1455 1842 Fragments arranged by size: 2875 1842 1455 1412 1010 991 838 809 808 686 631 627 583 547 458 377 329 325 320 224 217 189 186 150 124 123 120 77 77 17 BanII G_rGCy'C Cuts at: 1391 4110 4583 5052 5407 5460 5497 5691 6371 Size: 2719 473 469 355 53 37 194 680 Cuts at: 6371 6688 8473 8775 9271 9351 10131 10367 11073 Size: 317 1785 302 496 80 780 236 706 Cuts at: 11073 11143 11351 11596 11670 11743 11757 11853 12036 Size: 70 208 245 74 73 14 96 183 Cuts at: 12036 12064 12122 12344 12892 13157 13350 13605 13983 Size: 28 58 222 548 265 193 255 378 Cuts at: 13983 14078 14921 15285 15710 15746 1391 Size: 95 843 364 425 36 4072 Fragments arranged by size: 4072 2719 1785 843 780 706 680 548 496 473 469 425 378 364 355 317 302 265 255 245 236 222 208 194 193 183 96 95 80 74 73 70 58 53 37 36 28 14 BbeI G_GCGC'C Cuts at: 3628 5748 6739 8035 8155 14689 15375 3628 Size: 2120 991 1296 120 6534 686 6680 Fragments arranged by size: 6680 6534 2120 1296 991 686 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 3257 6854 7557 10149 12974 13891 Size: 971 396 962 3597 703 2592 2825 917 Cuts at: 13891 14477 928 Size: 586 4878 Fragments arranged by size: 4878 3597 2825 2592 971 962 917 703 586 396 BbvCI CC'TCA_GC Cuts at: 2022 2817 7407 7835 8043 8355 9066 9225 9282 Size: 795 4590 428 208 312 711 159 57 Cuts at: 9282 9849 12053 12732 13032 13649 2022 Size: 567 2204 679 300 617 6800 Fragments arranged by size: 6800 4590 2204 795 711 679 617 567 428 312 300 208 159 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4763 4797 5185 5219 6244 6278 9331 Size: 34 2826 34 388 34 1025 34 3053 Cuts at: 9331 9365 10159 10193 11041 11075 16618 16652 1903 Size: 34 794 34 848 34 5543 34 3678 Fragments arranged by size: 5543 3678 3053 2826 1025 848 794 388 34 34 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 16319 17529 16319 Size: 1210 17217 BclI T'GATC_A Cuts at: 1548 12939 14664 1548 Size: 11391 1725 5311 Fragments arranged by size: 11391 5311 1725 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3083 3762 4445 Size: 107 122 71 631 605 1411 679 683 Cuts at: 4445 4685 5441 5471 5981 6401 6489 6665 6692 Size: 240 756 30 510 420 88 176 27 Cuts at: 6692 6950 7004 7355 7397 7490 8207 8348 8738 Size: 258 54 351 42 93 717 141 390 Cuts at: 8738 8951 9911 11346 11384 11600 12159 12236 12326 Size: 213 960 1435 38 216 559 77 90 Cuts at: 12326 12377 12828 13241 13496 15146 15200 17038 136 Size: 51 451 413 255 1650 54 1838 1525 Fragments arranged by size: 1838 1650 1525 1435 1411 960 756 717 683 679 631 605 559 510 451 420 413 390 351 258 255 240 216 213 176 141 122 107 93 90 88 77 71 54 54 51 42 38 30 27 BglII A'GATC_T Cuts at: 2520 10440 15414 18422 2520 Size: 7920 4974 3008 2525 Fragments arranged by size: 7920 4974 3008 2525 BlpI GC'TnA_GC Cuts at: 2172 4610 4947 6138 6414 6915 8804 9231 9444 Size: 2438 337 1191 276 501 1889 427 213 Cuts at: 9444 11705 13377 15844 2172 Size: 2261 1672 2467 4755 Fragments arranged by size: 4755 2467 2438 2261 1889 1672 1191 501 427 337 276 213 Bme1580I G_kGCm'C Cuts at: 1790 3159 3811 4554 4583 4641 4662 5014 5100 Size: 1369 652 743 29 58 21 352 86 Cuts at: 5100 5146 5407 5497 5554 6526 6688 6928 7510 Size: 46 261 90 57 972 162 240 582 Cuts at: 7510 8536 10367 10545 10560 11283 11351 11544 11596 Size: 1026 1831 178 15 723 68 193 52 Cuts at: 11596 11743 12016 12344 12522 12892 13094 13350 14078 Size: 147 273 328 178 370 202 256 728 Cuts at: 14078 14921 15306 15461 15710 16488 17644 1790 Size: 843 385 155 249 778 1156 2573 Fragments arranged by size: 2573 1831 1369 1156 1026 972 843 778 743 728 723 652 582 385 370 352 328 273 261 256 249 240 202 193 178 178 162 155 147 90 86 68 58 57 52 46 29 21 15 BmgBI CAC'GTC Cuts at: 1318 2806 2887 3451 4792 5599 5674 10147 10567 Size: 1488 81 564 1341 807 75 4473 420 Cuts at: 10567 12544 15367 1318 Size: 1977 2823 4378 Fragments arranged by size: 4473 4378 2823 1977 1488 1341 807 564 420 81 75 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5708 12058 14130 Size: 416 53 83 399 24 4291 6350 2072 Cuts at: 14130 16158 17116 442 Size: 2028 958 1753 Fragments arranged by size: 6350 4291 2072 2028 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 18427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2350 4075 4978 6655 7438 8794 11776 12136 2350 Size: 1725 903 1677 783 1356 2982 360 8641 Fragments arranged by size: 8641 2982 1725 1677 1356 903 783 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 2623 2665 2753 2995 3143 3239 3278 Size: 1106 278 42 88 242 148 96 39 Cuts at: 3278 3656 3950 4036 4100 4385 4721 4892 5093 Size: 378 294 86 64 285 336 171 201 Cuts at: 5093 5306 5327 5360 5870 5921 6212 6719 6806 Size: 213 21 33 510 51 291 507 87 Cuts at: 6806 6881 6998 7187 7301 7472 7745 7823 7937 Size: 75 117 189 114 171 273 78 114 Cuts at: 7937 7943 8006 8129 8690 9209 9233 9407 9470 Size: 6 63 123 561 519 24 174 63 Cuts at: 9470 9653 9833 9908 10295 10523 10709 11066 11198 Size: 183 180 75 387 228 186 357 132 Cuts at: 11198 11654 12191 12272 12500 12686 13277 13565 14006 Size: 456 537 81 228 186 591 288 441 Cuts at: 14006 14234 14612 14857 14972 15041 15269 15497 17087 Size: 228 378 245 115 69 228 228 1590 Cuts at: 17087 1239 Size: 2579 Fragments arranged by size: 2579 1590 1106 591 561 537 519 510 507 456 441 387 378 378 357 336 294 291 288 285 278 273 245 242 228 228 228 228 228 213 201 189 186 186 183 180 174 171 171 148 132 123 117 115 114 114 96 88 87 86 81 78 75 75 69 64 63 63 51 42 39 33 24 21 6 Bpu10I CC'TnA_GC Cuts at: 2022 2817 5399 7407 7835 8043 8355 8472 9066 Size: 795 2582 2008 428 208 312 117 594 Cuts at: 9066 9225 9282 9849 12053 12732 13032 13649 13667 Size: 159 57 567 2204 679 300 617 18 Cuts at: 13667 2022 Size: 6782 Fragments arranged by size: 6782 2582 2204 2008 795 679 617 594 567 428 312 300 208 159 117 57 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3134 3446 5333 5405 5660 5984 6977 7613 7637 Size: 312 1887 72 255 324 993 636 24 Cuts at: 7637 8798 9005 9044 9224 9482 12062 13199 15395 Size: 1161 207 39 180 258 2580 1137 2196 Cuts at: 15395 16552 17417 17715 3134 Size: 1157 865 298 3846 Fragments arranged by size: 3846 2580 2196 1887 1161 1157 1137 993 865 636 324 312 298 258 255 207 180 72 39 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3045 8669 11576 11774 11966 12390 12737 Size: 617 1546 5624 2907 198 192 424 347 Cuts at: 12737 17090 882 Size: 4353 2219 Fragments arranged by size: 5624 4353 2907 2219 1546 617 424 347 198 192 BsaAI yAC'GTr Cuts at: 493 1204 3208 4747 6034 6886 10674 12651 13012 Size: 711 2004 1539 1287 852 3788 1977 361 Cuts at: 13012 13624 16218 493 Size: 612 2594 2702 Fragments arranged by size: 3788 2702 2594 2004 1977 1539 1287 852 711 612 361 BsaBI GATnn'nnATC Cuts at: 3029 10961 12944 14630 18421 3029 Size: 7932 1983 1686 3791 3035 Fragments arranged by size: 7932 3791 3035 1983 1686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2865 3333 3625 5526 Size: 53 83 186 936 1332 468 292 1901 Cuts at: 5526 5745 5760 6736 8032 8152 9291 10794 11490 Size: 219 15 976 1296 120 1139 1503 696 Cuts at: 11490 11616 13722 13908 14474 14686 15087 15339 15372 Size: 126 2106 186 566 212 401 252 33 Cuts at: 15372 15606 16234 16616 17251 275 Size: 234 628 382 635 1451 Fragments arranged by size: 2106 1901 1503 1451 1332 1296 1139 976 936 696 635 628 566 468 401 382 292 252 234 219 212 186 186 126 120 83 53 33 15 BsaWI w'CCGG_w Cuts at: 9303 14003 14982 15062 16860 17258 17532 17679 18090 Size: 4700 979 80 1798 398 274 147 411 Cuts at: 18090 9303 Size: 9640 Fragments arranged by size: 9640 4700 1798 979 411 398 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2356 2386 2621 Size: 30 165 30 897 30 26 30 235 Cuts at: 2621 2651 3176 3206 3959 3989 7211 7241 8162 Size: 30 525 30 753 30 3222 30 921 Cuts at: 8162 8192 10763 10793 11978 12008 12176 12206 12815 Size: 30 2571 30 1185 30 168 30 609 Cuts at: 12815 12845 13090 13120 14600 14630 14663 14693 14945 Size: 30 245 30 1480 30 33 30 252 Cuts at: 14945 14975 15242 15272 1178 Size: 30 267 30 4333 Fragments arranged by size: 4333 3222 2571 1480 1185 921 897 753 609 525 267 252 245 235 168 165 33 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3035 3326 3698 3895 4007 4010 4013 4859 Size: 1849 291 372 197 112 3 3 846 Cuts at: 4859 5405 5426 5867 5897 6134 6137 6140 6170 Size: 546 21 441 30 237 3 3 30 Cuts at: 6170 6296 6314 6359 6452 6455 6548 6581 6716 Size: 126 18 45 93 3 93 33 135 Cuts at: 6716 6737 6923 6974 7046 7100 7139 7298 7301 Size: 21 186 51 72 54 39 159 3 Cuts at: 7301 7424 7427 7502 7742 7775 7871 7874 8033 Size: 123 3 75 240 33 96 3 159 Cuts at: 8033 8036 8060 8090 8093 8156 8372 8381 8465 Size: 3 24 30 3 63 216 9 84 Cuts at: 8465 8687 8720 8780 8783 8813 8903 8933 8993 Size: 222 33 60 3 30 90 30 60 Cuts at: 8993 9176 9299 9332 9521 9695 9758 9788 9812 Size: 183 123 33 189 174 63 30 24 Cuts at: 9812 9851 9866 9905 9938 10100 10610 10991 11195 Size: 39 15 39 33 162 510 381 204 Cuts at: 11195 11594 11765 11978 12587 12779 12782 12893 13481 Size: 399 171 213 609 192 3 111 588 Cuts at: 13481 13784 13856 14063 14267 14321 14387 14626 14629 Size: 303 72 207 204 54 66 239 3 Cuts at: 14629 14636 14818 14833 14836 14924 15467 15643 15646 Size: 7 182 15 3 88 543 176 3 Cuts at: 15646 1186 Size: 3967 Fragments arranged by size: 3967 1849 846 609 588 546 543 510 441 399 381 372 303 291 240 239 237 222 216 213 207 204 204 197 192 189 186 183 182 176 174 171 162 159 159 135 126 123 123 112 111 96 93 93 90 88 84 75 72 72 66 63 63 60 60 54 54 51 45 39 39 39 33 33 33 33 33 30 30 30 30 30 30 24 24 21 21 18 15 15 9 7 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2072 2141 2167 3731 3748 4118 4480 4707 5145 Size: 69 26 1564 17 370 362 227 438 Cuts at: 5145 5191 5296 5631 7773 7927 9294 9666 10622 Size: 46 105 335 2142 154 1367 372 956 Cuts at: 10622 11493 11522 11636 12115 12599 12891 13491 13604 Size: 871 29 114 479 484 292 600 113 Cuts at: 13604 14880 15056 17630 18126 2072 Size: 1276 176 2574 496 2373 Fragments arranged by size: 2574 2373 2142 1564 1367 1276 956 871 600 496 484 479 438 372 370 362 335 292 227 176 154 114 113 105 69 46 29 26 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2495 2909 3188 3473 3500 3638 Size: 573 498 261 414 279 285 27 138 Cuts at: 3638 3752 4481 4569 4814 4847 4862 5751 6614 Size: 114 729 88 245 33 15 889 863 Cuts at: 6614 6825 8075 8279 8399 8810 9062 9320 9398 Size: 211 1250 204 120 411 252 258 78 Cuts at: 9398 9542 10358 13340 15019 15275 1163 Size: 144 816 2982 1679 256 4315 Fragments arranged by size: 4315 2982 1679 1250 889 863 816 729 573 498 414 411 285 279 261 258 256 252 245 211 204 144 138 120 114 88 78 33 27 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 2985 4024 7468 7899 8073 Size: 275 186 655 329 1039 3444 431 174 Cuts at: 8073 10395 11065 12919 13363 14106 14674 14731 15195 Size: 2322 670 1854 444 743 568 57 464 Cuts at: 15195 15670 15877 16638 17666 1540 Size: 475 207 761 1028 2301 Fragments arranged by size: 3444 2322 2301 1854 1039 1028 761 743 670 655 568 475 464 444 431 329 275 207 186 174 57 BsiHKAI G_wGCw'C Cuts at: 4110 4554 4611 5014 5052 5100 5400 5460 5691 Size: 444 57 403 38 48 300 60 231 Cuts at: 5691 6371 6526 8775 9351 10017 10131 10488 10545 Size: 680 155 2249 576 666 114 357 57 Cuts at: 10545 11073 11670 11757 11853 12036 12522 13983 16488 Size: 528 597 87 96 183 486 1461 2505 Cuts at: 16488 16573 17644 4110 Size: 85 1071 4893 Fragments arranged by size: 4893 2505 2249 1461 1071 680 666 597 576 528 486 444 403 357 300 231 183 155 114 96 87 85 60 57 57 48 38 BsiWI C'GTAC_G Cuts at: 6044 6044 Size: 18427 BsmI GAATG_Cn' Cuts at: 6336 15938 16031 6336 Size: 9602 93 8732 Fragments arranged by size: 9602 8732 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2643 2854 5078 6782 7241 9158 9539 10706 11103 Size: 211 2224 1704 459 1917 381 1167 397 Cuts at: 11103 11431 12683 14519 14875 14906 14939 2643 Size: 328 1252 1836 356 31 33 6131 Fragments arranged by size: 6131 2224 1917 1836 1704 1252 1167 459 397 381 356 328 211 33 31 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3159 3811 4110 4554 4583 4611 4641 Size: 399 1369 652 299 444 29 28 30 Cuts at: 4641 4662 5014 5052 5100 5146 5400 5407 5460 Size: 21 352 38 48 46 254 7 53 Cuts at: 5460 5497 5554 5691 6371 6526 6688 6928 7510 Size: 37 57 137 680 155 162 240 582 Cuts at: 7510 8473 8536 8775 9271 9351 10017 10131 10367 Size: 963 63 239 496 80 666 114 236 Cuts at: 10367 10488 10545 10560 11073 11143 11283 11351 11544 Size: 121 57 15 513 70 140 68 193 Cuts at: 11544 11596 11670 11743 11757 11853 12016 12036 12064 Size: 52 74 73 14 96 163 20 28 Cuts at: 12064 12122 12344 12522 12892 13094 13157 13350 13605 Size: 58 222 178 370 202 63 193 255 Cuts at: 13605 13983 14078 14921 15285 15306 15461 15710 15746 Size: 378 95 843 364 21 155 249 36 Cuts at: 15746 16488 16573 17644 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1369 1071 963 843 742 680 666 652 582 513 496 444 399 378 370 364 352 299 255 254 249 240 239 236 222 202 193 193 178 163 162 155 155 140 137 121 114 96 95 85 80 74 73 70 68 63 63 58 57 57 53 52 48 46 38 37 36 30 29 28 28 21 21 20 15 14 7 BspEI T'CCGG_A Cuts at: 9303 18090 9303 Size: 8787 9640 BspHI T'CATG_A Cuts at: 11359 16316 18046 11359 Size: 4957 1730 11740 Fragments arranged by size: 11740 4957 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2991 3666 3675 3735 3888 4380 4525 5302 Size: 2147 675 9 60 153 492 145 777 Cuts at: 5302 6048 6580 6760 7840 8404 8623 8703 8899 Size: 746 532 180 1080 564 219 80 196 Cuts at: 8899 9931 10167 10686 10914 11235 12742 13086 13182 Size: 1032 236 519 228 321 1507 344 96 Cuts at: 13182 13777 14397 15401 15782 844 Size: 595 620 1004 381 3489 Fragments arranged by size: 3489 2147 1507 1080 1032 1004 777 746 675 620 595 564 532 519 492 381 344 321 236 228 219 196 180 153 145 96 80 60 9 BsrBI CCG'CTC Cuts at: 1394 1823 2176 2203 2767 3131 3223 3805 4806 Size: 429 353 27 564 364 92 582 1001 Cuts at: 4806 5593 5623 6166 6247 6733 6919 6949 6970 Size: 787 30 543 81 486 186 30 21 Cuts at: 6970 7582 7807 8026 8122 8168 8431 9199 11719 Size: 612 225 219 96 46 263 768 2520 Cuts at: 11719 12670 13306 13381 14467 14679 14733 14817 16314 Size: 951 636 75 1086 212 54 84 1497 Cuts at: 16314 1394 Size: 3507 Fragments arranged by size: 3507 2520 1497 1086 1001 951 787 768 636 612 582 564 543 486 429 364 353 263 225 219 212 186 96 92 84 81 75 54 46 30 30 27 21 BsrDI GCAATG_nn' Cuts at: 65 1248 16922 17096 65 Size: 1183 15674 174 1396 Fragments arranged by size: 15674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2679 2718 4125 4935 5128 5440 Size: 110 311 558 39 1407 810 193 312 Cuts at: 5440 5968 6423 7463 7467 10067 10320 10575 10995 Size: 528 455 1040 4 2600 253 255 420 Cuts at: 10995 11270 11650 11682 12158 12386 12914 13391 13502 Size: 275 380 32 476 228 528 477 111 Cuts at: 13502 13694 14003 14262 14756 14804 15062 15233 15266 Size: 192 309 259 494 48 258 171 33 Cuts at: 15266 15557 15569 15581 15611 17071 17258 1700 Size: 291 12 12 30 1460 187 2869 Fragments arranged by size: 2869 2600 1460 1407 1040 810 558 528 528 494 477 476 455 420 380 312 311 309 291 275 259 258 255 253 228 193 192 187 171 111 110 48 39 33 32 30 12 12 4 BsrGI T'GTAC_A Cuts at: 96 12756 96 Size: 12660 5767 BssHII G'CGCG_C Cuts at: 1925 6668 6953 10399 14110 1925 Size: 4743 285 3446 3711 6242 Fragments arranged by size: 6242 4743 3711 3446 285 BssSI C'ACGA_G Cuts at: 3518 5408 10661 12314 12353 13340 16487 17499 3518 Size: 1890 5253 1653 39 987 3147 1012 4446 Fragments arranged by size: 5253 4446 3147 1890 1653 1012 987 39 BstAPI GCAn_nnn'nTGC Cuts at: 794 3616 3667 4306 4741 4990 5104 5656 5845 Size: 2822 51 639 435 249 114 552 189 Cuts at: 5845 6274 6358 6517 6521 8290 8308 8617 9040 Size: 429 84 159 4 1769 18 309 423 Cuts at: 9040 9520 9538 9556 11333 11476 12150 13884 15016 Size: 480 18 18 1777 143 674 1734 1132 Cuts at: 15016 794 Size: 4205 Fragments arranged by size: 4205 2822 1777 1769 1734 1132 674 639 552 480 435 429 423 309 249 189 159 143 114 84 51 18 18 18 4 BstBI TT'CG_AA Cuts at: 17231 17231 Size: 18427 BstEII G'GTnAC_C Cuts at: 1184 9367 10849 11920 12922 13387 13846 1184 Size: 8183 1482 1071 1002 465 459 5765 Fragments arranged by size: 8183 5765 1482 1071 1002 465 459 BstXI CCAn_nnnn'nTGG Cuts at: 2540 4118 5338 5362 5797 2540 Size: 1578 1220 24 435 15170 Fragments arranged by size: 15170 1578 1220 435 24 BstYI r'GATC_y Cuts at: 1972 2520 3030 5781 6381 6444 7863 9390 10440 Size: 548 510 2751 600 63 1419 1527 1050 Cuts at: 10440 14319 14837 15414 15757 16124 16509 16526 17978 Size: 3879 518 577 343 367 385 17 1452 Cuts at: 17978 18064 18076 18422 1972 Size: 86 12 346 1977 Fragments arranged by size: 3879 2751 1977 1527 1452 1419 1050 600 577 548 518 510 385 367 346 343 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2799 3920 10291 14250 2799 Size: 1121 6371 3959 6976 Fragments arranged by size: 6976 6371 3959 1121 BtgI C'CryG_G Cuts at: 513 1065 4158 5371 5866 7215 8733 9993 10116 Size: 552 3093 1213 495 1349 1518 1260 123 Cuts at: 10116 10518 10560 11247 11316 11502 11811 11925 12495 Size: 402 42 687 69 186 309 114 570 Cuts at: 12495 12823 13158 13741 13764 13803 14197 14229 14271 Size: 328 335 583 23 39 394 32 42 Cuts at: 14271 14922 15592 15720 513 Size: 651 670 128 3220 Fragments arranged by size: 3220 3093 1518 1349 1260 1213 687 670 651 583 570 552 495 402 394 335 328 309 186 128 123 114 69 42 42 39 32 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2176 2313 2443 2635 5137 5238 Size: 683 56 950 137 130 192 2502 101 Cuts at: 5238 9424 11146 13873 14695 487 Size: 4186 1722 2727 822 4219 Fragments arranged by size: 4219 4186 2727 2502 1722 950 822 683 192 137 130 101 56 BtsI GCAGTG_nn' Cuts at: 2068 2087 4199 4316 6515 6808 8654 9930 11334 Size: 19 2112 117 2199 293 1846 1276 1404 Cuts at: 11334 11871 12151 15943 16737 16757 2068 Size: 537 280 3792 794 20 3738 Fragments arranged by size: 3792 3738 2199 2112 1846 1404 1276 794 537 293 280 117 20 19 ClaI AT'CG_AT Cuts at: 1554 14700 1554 Size: 13146 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3573 3608 14788 14823 529 Size: 35 3009 35 11180 35 4133 Fragments arranged by size: 11180 4133 3009 35 35 35 DraI TTT'AAA Cuts at: 15731 15731 Size: 18427 DraIII CAC_nnn'GTG Cuts at: 2235 3700 3815 4731 6521 10555 11539 12532 2235 Size: 1465 115 916 1790 4034 984 993 8130 Fragments arranged by size: 8130 4034 1790 1465 993 984 916 115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2024 2810 14651 15187 17434 809 Size: 507 708 786 11841 536 2247 1802 Fragments arranged by size: 11841 2247 1802 786 708 536 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2562 2653 Size: 54 1425 50 275 18 472 260 91 Cuts at: 2653 2982 3210 4437 4515 4818 5442 5799 5982 Size: 329 228 1227 78 303 624 357 183 Cuts at: 5982 6171 6210 7212 7356 7389 7465 7482 7542 Size: 189 39 1002 144 33 76 17 60 Cuts at: 7542 7602 7896 8070 9252 9657 9732 9970 10392 Size: 60 294 174 1182 405 75 238 422 Cuts at: 10392 10432 11244 11652 12264 12916 13497 13696 13998 Size: 40 812 408 612 652 581 199 302 Cuts at: 13998 14103 15192 15235 15667 15723 15874 16763 18392 Size: 105 1089 43 432 56 151 889 1629 Cuts at: 18392 8 Size: 43 Fragments arranged by size: 1629 1425 1227 1182 1089 1002 889 812 652 624 612 581 472 432 422 408 405 357 329 303 302 294 275 260 238 228 199 189 183 174 151 144 105 91 78 76 75 60 60 56 54 50 43 43 40 39 33 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2653 2982 7465 7896 8070 10392 12916 Size: 275 841 329 4483 431 174 2322 2524 Cuts at: 12916 14103 15192 15667 15874 1537 Size: 1187 1089 475 207 4090 Fragments arranged by size: 4483 4090 2524 2322 1187 1089 841 475 431 329 275 207 174 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3446 4751 5013 7043 7512 8054 8229 Size: 1313 780 1305 262 2030 469 542 175 Cuts at: 8229 8415 8780 9647 10454 12431 12783 13436 14165 Size: 186 365 867 807 1977 352 653 729 Cuts at: 14165 14303 15643 16357 1353 Size: 138 1340 714 3423 Fragments arranged by size: 3423 2030 1977 1340 1313 1305 867 807 780 729 714 653 542 469 365 352 262 186 175 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2777 3434 4336 6257 6392 6545 Size: 1064 138 1188 657 902 1921 135 153 Cuts at: 6545 6554 6638 6734 6743 6827 7043 7106 7136 Size: 9 84 96 9 84 216 63 30 Cuts at: 7136 7202 7529 7592 7655 7682 7709 7925 8030 Size: 66 327 63 63 27 27 216 105 Cuts at: 8030 8246 8348 8378 8399 8555 8741 8930 9086 Size: 216 102 30 21 156 186 189 156 Cuts at: 9086 9173 9329 10051 10484 10631 12461 12608 12737 Size: 87 156 722 433 147 1830 147 129 Cuts at: 12737 12971 13117 13136 13949 14195 17014 17388 17534 Size: 234 146 19 813 246 2819 374 146 Cuts at: 17534 387 Size: 1280 Fragments arranged by size: 2819 1921 1830 1280 1188 1064 902 813 722 657 433 374 327 246 234 216 216 216 189 186 156 156 156 153 147 147 146 146 138 135 129 105 102 96 87 84 84 66 63 63 63 30 30 27 27 21 19 9 9 EcoICRI GAG'CTC Cuts at: 4108 5050 5458 5689 6369 8773 9349 10129 11071 Size: 942 408 231 680 2404 576 780 942 Cuts at: 11071 11668 11755 11851 12034 13981 4108 Size: 597 87 96 183 1947 8554 Fragments arranged by size: 8554 2404 1947 942 942 780 680 597 576 408 231 183 96 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2345 2623 2665 2753 2945 2995 Size: 393 429 284 278 42 88 192 50 Cuts at: 2995 3071 3143 3239 3251 3278 3581 3656 3950 Size: 76 72 96 12 27 303 75 294 Cuts at: 3950 4036 4100 4106 4259 4385 4487 4721 4856 Size: 86 64 6 153 126 102 234 135 Cuts at: 4856 4892 5093 5306 5327 5360 5501 5870 5921 Size: 36 201 213 21 33 141 369 51 Cuts at: 5921 6212 6719 6806 6881 6998 7187 7301 7472 Size: 291 507 87 75 117 189 114 171 Cuts at: 7472 7745 7823 7895 7937 7943 8006 8129 8456 Size: 273 78 72 42 6 63 123 327 Cuts at: 8456 8690 8741 9209 9233 9407 9470 9653 9833 Size: 234 51 468 24 174 63 183 180 Cuts at: 9833 9908 10214 10295 10523 10709 11066 11198 11237 Size: 75 306 81 228 186 357 132 39 Cuts at: 11237 11465 11654 11983 12191 12272 12500 12550 12686 Size: 228 189 329 208 81 228 50 136 Cuts at: 12686 13277 13565 14006 14054 14210 14234 14468 14612 Size: 591 288 441 48 156 24 234 144 Cuts at: 14612 14857 14972 15041 15227 15269 15497 16490 17087 Size: 245 115 69 186 42 228 993 597 Cuts at: 17087 17874 18142 1239 Size: 787 268 1524 Fragments arranged by size: 1524 993 787 597 591 507 468 441 429 393 369 357 329 327 306 303 294 291 288 284 278 273 268 245 234 234 234 228 228 228 228 213 208 201 192 189 189 186 186 183 180 174 171 156 153 144 141 136 135 132 126 123 117 115 114 102 96 88 87 86 81 81 78 76 75 75 75 72 72 69 64 63 63 51 51 50 50 48 42 42 42 39 36 33 27 24 24 21 12 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 2255 2324 2744 4376 5003 5849 6743 6875 7058 Size: 69 420 1632 627 846 894 132 183 Cuts at: 7058 8885 9431 10274 10502 11435 11447 11576 11981 Size: 1827 546 843 228 933 12 129 405 Cuts at: 11981 12251 12428 12479 12848 13043 13106 13574 14162 Size: 270 177 51 369 195 63 468 588 Cuts at: 14162 14213 15209 15311 2255 Size: 51 996 102 5371 Fragments arranged by size: 5371 1827 1632 996 933 894 846 843 627 588 546 468 420 405 369 270 228 195 183 177 132 129 102 69 63 51 51 12 EcoO109I rG'GnC_Cy Cuts at: 2329 2614 2770 3664 3924 4369 4580 4717 5403 Size: 285 156 894 260 445 211 137 686 Cuts at: 5403 5842 6685 6694 8983 9553 10364 10420 10468 Size: 439 843 9 2289 570 811 56 48 Cuts at: 10468 10705 10903 11011 11348 11404 11440 11452 11473 Size: 237 198 108 337 56 36 12 21 Cuts at: 11473 11592 11593 11806 12049 12103 12217 12341 12445 Size: 119 1 213 243 54 114 124 104 Cuts at: 12445 12682 13036 13336 13346 13471 13825 14075 14131 Size: 237 354 300 10 125 354 250 56 Cuts at: 14131 14179 14566 14877 14918 15100 15166 15202 15316 Size: 48 387 311 41 182 66 36 114 Cuts at: 15316 15687 15706 15707 2329 Size: 371 19 1 5049 Fragments arranged by size: 5049 2289 894 843 811 686 570 445 439 387 371 354 354 337 311 300 285 260 250 243 237 237 213 211 198 182 156 137 125 124 119 114 114 108 104 66 56 56 56 54 48 48 41 36 36 21 19 12 10 9 1 1 EcoRI G'AATT_C Cuts at: 3785 15736 16114 3785 Size: 11951 378 6098 Fragments arranged by size: 11951 6098 378 EcoRV GAT'ATC Cuts at: 2530 2530 Size: 18427 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2242 7900 10660 12637 13753 14371 2242 Size: 5658 2760 1977 1116 618 6298 Fragments arranged by size: 6298 5658 2760 1977 1116 618 FseI GG_CCGG'CC Cuts at: 7467 12918 15237 7467 Size: 5451 2319 10657 Fragments arranged by size: 10657 5451 2319 FspI TGC'GCA Cuts at: 6431 7781 7964 8501 9102 11018 13616 14495 16933 Size: 1350 183 537 601 1916 2598 879 2438 Cuts at: 16933 6431 Size: 7925 Fragments arranged by size: 7925 2598 2438 1916 1350 879 601 537 183 HaeII r_GCGC'y Cuts at: 1678 2142 3526 3628 3711 4067 4855 5038 5614 Size: 464 1384 102 83 356 788 183 576 Cuts at: 5614 5647 5748 5866 6484 6580 6739 6868 6985 Size: 33 101 118 618 96 159 129 117 Cuts at: 6985 7010 7210 7681 7768 7874 8035 8108 8155 Size: 25 200 471 87 106 161 73 47 Cuts at: 8155 8878 9225 9238 9301 9427 9607 10696 11101 Size: 723 347 13 63 126 180 1089 405 Cuts at: 11101 11307 12304 13648 13811 14503 14689 15073 15244 Size: 206 997 1344 163 692 186 384 171 Cuts at: 15244 15375 17574 1678 Size: 131 2199 2531 Fragments arranged by size: 2531 2199 1384 1344 1089 997 788 723 692 618 576 471 464 405 384 356 347 206 200 186 183 180 171 163 161 159 131 129 126 118 117 106 102 101 96 87 83 73 63 47 33 25 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2816 2848 7400 7432 8162 Size: 32 135 32 1244 32 4552 32 730 Cuts at: 8162 8194 11978 12010 12176 12208 12389 12421 13045 Size: 32 3784 32 166 32 181 32 624 Cuts at: 13045 13077 13109 13141 13580 13612 14123 14155 14496 Size: 32 32 32 439 32 511 32 341 Cuts at: 14496 14528 14663 14695 14777 14809 14945 14977 17068 Size: 32 135 32 82 32 136 32 2091 Cuts at: 17068 17100 17142 17174 1373 Size: 32 42 32 2626 Fragments arranged by size: 4552 3784 2626 2091 1244 730 624 511 439 341 181 166 136 135 135 82 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 10336 15094 15771 16017 669 Size: 988 281 304 8094 4758 677 246 3079 Fragments arranged by size: 8094 4758 3079 988 677 304 281 246 HindIII A'AGCT_T Cuts at: 748 9565 748 Size: 8817 9610 HpaI GTT'AAC Cuts at: 16017 16017 Size: 18427 KasI G'GCGC_C Cuts at: 3624 5744 6735 8031 8151 14685 15371 3624 Size: 2120 991 1296 120 6534 686 6680 Fragments arranged by size: 6680 6534 2120 1296 991 686 120 KpnI G_GTAC'C Cuts at: 2611 15752 2611 Size: 13141 5286 MfeI C'AATT_G Cuts at: 16026 16026 Size: 18427 MluI A'CGCG_T Cuts at: 18303 18303 Size: 18427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 3327 6447 9496 11238 11262 11466 12843 Size: 1224 798 3120 3049 1742 24 204 1377 Cuts at: 12843 13698 17541 17725 1305 Size: 855 3843 184 2007 Fragments arranged by size: 3843 3120 3049 2007 1742 1377 1224 855 798 204 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 2304 3212 4439 4820 7358 Size: 54 1425 343 472 908 1227 381 2538 Cuts at: 7358 7391 9659 9734 10434 13499 10 Size: 33 2268 75 700 3065 4938 Fragments arranged by size: 4938 3065 2538 2268 1425 1227 908 700 472 381 343 75 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2702 4034 4557 5137 10118 10967 Size: 648 1108 428 1332 523 580 4981 849 Cuts at: 10967 10970 11246 12752 16385 16744 16903 518 Size: 3 276 1506 3633 359 159 2042 Fragments arranged by size: 4981 3633 2042 1506 1332 1108 849 648 580 523 428 359 276 159 3 MspA1I CmG'CkG Cuts at: 1606 2116 2130 2171 2643 2920 3175 3319 3385 Size: 510 14 41 472 277 255 144 66 Cuts at: 3385 3748 4213 4405 4465 4573 4987 5071 5116 Size: 363 465 192 60 108 414 84 45 Cuts at: 5116 5373 5581 5635 5710 5797 5827 5941 6148 Size: 257 208 54 75 87 30 114 207 Cuts at: 6148 6202 6208 6358 6682 6859 6901 7144 7177 Size: 54 6 150 324 177 42 243 33 Cuts at: 7177 7183 7217 7270 7276 7534 7540 7567 7714 Size: 6 34 53 6 258 6 27 147 Cuts at: 7714 7777 7843 7901 8002 8008 8095 8191 8197 Size: 63 66 58 101 6 87 96 6 Cuts at: 8197 8320 8573 8668 8716 8971 8998 9154 9181 Size: 123 253 95 48 255 27 156 27 Cuts at: 9181 9205 9211 9535 9598 9673 9691 9724 9811 Size: 24 6 324 63 75 18 33 87 Cuts at: 9811 9823 9877 9995 10096 10243 10897 11083 11497 Size: 12 54 118 101 147 654 186 414 Cuts at: 11497 11677 11695 11884 12003 12361 12661 12886 12895 Size: 180 18 189 119 358 300 225 9 Cuts at: 12895 13495 13594 13663 13702 13816 13884 14395 14446 Size: 600 99 69 39 114 68 511 51 Cuts at: 14446 14884 15013 15528 15545 15594 15843 16520 17668 Size: 438 129 515 17 49 249 677 1148 Cuts at: 17668 17913 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 654 600 515 511 510 472 465 438 414 414 363 358 324 324 300 277 258 257 255 255 253 249 245 243 225 208 207 192 189 186 180 177 156 150 147 147 144 129 123 119 118 114 114 108 101 101 99 96 95 87 87 87 84 75 75 69 68 66 66 63 63 60 58 54 54 54 53 51 49 48 45 42 41 39 34 33 33 30 27 27 27 24 18 18 17 14 12 9 6 6 6 6 6 6 6 NaeI GCC'GGC Cuts at: 2123 5130 5442 6425 7465 10069 10997 11272 11652 Size: 3007 312 983 1040 2604 928 275 380 Cuts at: 11652 11684 12160 12916 14758 15235 15583 2123 Size: 32 476 756 1842 477 348 4967 Fragments arranged by size: 4967 3007 2604 1842 1040 983 928 756 477 476 380 348 312 275 32 NarI GG'CG_CC Cuts at: 3625 5745 6736 8032 8152 14686 15372 3625 Size: 2120 991 1296 120 6534 686 6680 Fragments arranged by size: 6680 6534 2120 1296 991 686 120 NcoI C'CATG_G Cuts at: 513 1065 13764 14197 513 Size: 552 12699 433 4743 Fragments arranged by size: 12699 4743 552 433 NdeI CA'TA_TG Cuts at: 387 387 Size: 18427 NgoMIV G'CCGG_C Cuts at: 2121 5128 5440 6423 7463 10067 10995 11270 11650 Size: 3007 312 983 1040 2604 928 275 380 Cuts at: 11650 11682 12158 12914 14756 15233 15581 2121 Size: 32 476 756 1842 477 348 4967 Fragments arranged by size: 4967 3007 2604 1842 1040 983 928 756 477 476 380 348 312 275 32 NheI G'CTAG_C Cuts at: 1052 1052 Size: 18427 NotI GC'GGCC_GC Cuts at: 7896 15874 7896 Size: 7978 10449 NspI r_CATG'y Cuts at: 1132 1229 6277 14791 15785 17330 1132 Size: 97 5048 8514 994 1545 2229 Fragments arranged by size: 8514 5048 2229 1545 994 97 PasI CC'CwG_GG Cuts at: 1348 2078 15700 1348 Size: 730 13622 4075 Fragments arranged by size: 13622 4075 730 PciI A'CATG_T Cuts at: 17326 17326 Size: 18427 PflMI CCAn_nnn'nTGG Cuts at: 1108 3121 3175 4576 4720 12898 1108 Size: 2013 54 1401 144 8178 6637 Fragments arranged by size: 8178 6637 2013 1401 144 54 PfoI T'CCnGG_A Cuts at: 2160 2790 3216 5451 6024 9645 9885 10479 13083 Size: 630 426 2235 573 3621 240 594 2604 Cuts at: 13083 13869 13983 14873 14934 15246 2160 Size: 786 114 890 61 312 5341 Fragments arranged by size: 5341 3621 2604 2235 890 786 630 594 573 426 312 240 114 61 PmeI GTTT'AAAC Cuts at: 15731 15731 Size: 18427 PmlI CAC'GTG Cuts at: 3208 4747 6886 3208 Size: 1539 2139 14749 Fragments arranged by size: 14749 2139 1539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 13025 13057 16496 16528 18009 18041 13025 Size: 32 3439 32 1481 32 13411 Fragments arranged by size: 13411 3439 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 2770 3664 3924 4369 8983 10420 10705 10903 Size: 441 894 260 445 4614 1437 285 198 Cuts at: 10903 11404 11806 12049 12103 12682 13036 14131 14877 Size: 501 402 243 54 579 354 1095 746 Cuts at: 14877 15166 15687 2329 Size: 289 521 5069 Fragments arranged by size: 5069 4614 1437 1095 894 746 579 521 501 445 441 402 354 289 285 260 243 198 54 PshAI GACnn'nnGTC Cuts at: 1613 12095 1613 Size: 10482 7945 PsiI TTA'TAA Cuts at: 15997 15997 Size: 18427 PspOMI G'GGCC_C Cuts at: 4579 5403 5493 6684 10363 11347 11592 11739 12340 Size: 824 90 1191 3679 984 245 147 601 Cuts at: 12340 12888 13346 14074 14917 15706 4579 Size: 548 458 728 843 789 7300 Fragments arranged by size: 7300 3679 1191 984 843 824 789 728 601 548 458 245 147 90 PspXI vC'TCGA_Gb Cuts at: 5459 5690 5459 Size: 231 18196 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2699 2731 4238 4270 6074 6106 10658 10690 12635 Size: 32 1507 32 1804 32 4552 32 1945 Cuts at: 12635 12667 14369 14401 2699 Size: 32 1702 32 6725 Fragments arranged by size: 6725 4552 1945 1804 1702 1507 32 32 32 32 32 32 PstI C_TGCA'G Cuts at: 830 2388 2919 3291 3345 3370 3612 3663 3885 Size: 1558 531 372 54 25 242 51 222 Cuts at: 3885 4188 4311 4380 4970 4995 5070 5577 5757 Size: 303 123 69 590 25 75 507 180 Cuts at: 5757 5832 6267 6354 6465 6513 6693 6717 6822 Size: 75 435 87 111 48 180 24 105 Cuts at: 6822 6876 7026 7305 7321 8196 8229 8295 8491 Size: 54 150 279 16 875 33 66 196 Cuts at: 8491 8667 8715 8886 9084 9126 9495 9543 9561 Size: 176 48 171 198 42 369 48 18 Cuts at: 9561 9807 9882 10062 10095 10164 10236 10683 11088 Size: 246 75 180 33 69 72 447 405 Cuts at: 11088 11772 12141 13815 14394 15324 15779 830 Size: 684 369 1674 579 930 455 3478 Fragments arranged by size: 3478 1674 1558 930 875 684 590 579 531 507 455 447 435 405 372 369 369 303 279 246 242 222 198 196 180 180 180 176 171 150 123 111 105 87 75 75 75 72 69 69 66 54 54 51 48 48 48 42 33 33 25 25 24 18 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 18427 PvuII CAG'CTG Cuts at: 2171 2643 2920 3319 3748 4213 4405 4465 4573 Size: 472 277 399 429 465 192 60 108 Cuts at: 4573 4987 5071 5635 5710 5827 5941 6208 6358 Size: 414 84 564 75 117 114 267 150 Cuts at: 6358 6859 6901 7183 7276 7540 7777 8008 8191 Size: 501 42 282 93 264 237 231 183 Cuts at: 8191 8197 8320 8573 8668 8971 9154 9211 9535 Size: 6 123 253 95 303 183 57 324 Cuts at: 9535 9673 9811 9877 10243 11083 11497 11695 12361 Size: 138 138 66 366 840 414 198 666 Cuts at: 12361 12661 12895 13495 13594 13663 13702 13816 13884 Size: 300 234 600 99 69 39 114 68 Cuts at: 13884 14395 14884 2171 Size: 511 489 5714 Fragments arranged by size: 5714 840 666 600 564 511 501 489 472 465 429 414 414 399 366 324 303 300 282 277 267 264 253 237 234 231 198 192 183 183 150 138 138 123 117 114 114 108 99 95 93 84 75 69 68 66 60 57 42 39 6 SacI G_AGCT'C Cuts at: 4110 5052 5460 5691 6371 8775 9351 10131 11073 Size: 942 408 231 680 2404 576 780 942 Cuts at: 11073 11670 11757 11853 12036 13983 4110 Size: 597 87 96 183 1947 8554 Fragments arranged by size: 8554 2404 1947 942 942 780 680 597 576 408 231 183 96 87 SacII CC_GC'GG Cuts at: 5374 7218 9996 15595 5374 Size: 1844 2778 5599 8206 Fragments arranged by size: 8206 5599 2778 1844 SalI G'TCGA_C Cuts at: 1936 15769 1936 Size: 13833 4594 SanDI GG'GwC_CC Cuts at: 11404 11404 Size: 18427 SapI GCTCTTCn'nnn_ Cuts at: 3446 4751 7043 8054 8780 9647 13436 3446 Size: 1305 2292 1011 726 867 3789 8437 Fragments arranged by size: 8437 3789 2292 1305 1011 867 726 SbfI CC_TGCA'GG Cuts at: 3663 6693 6876 8886 10164 15779 3663 Size: 3030 183 2010 1278 5615 6311 Fragments arranged by size: 6311 5615 3030 2010 1278 183 ScaI AGT'ACT Cuts at: 11867 16675 18387 11867 Size: 4808 1712 11907 Fragments arranged by size: 11907 4808 1712 SexAI A'CCwGG_T Cuts at: 14988 14988 Size: 18427 SfcI C'TryA_G Cuts at: 826 1045 2384 2915 3287 3341 3366 3608 3659 Size: 219 1339 531 372 54 25 242 51 Cuts at: 3659 3881 4184 4208 4307 4376 4966 4991 5066 Size: 222 303 24 99 69 590 25 75 Cuts at: 5066 5573 5753 5828 6052 6263 6350 6461 6509 Size: 507 180 75 224 211 87 111 48 Cuts at: 6509 6689 6713 6818 6872 7022 7301 7317 8192 Size: 180 24 105 54 150 279 16 875 Cuts at: 8192 8225 8279 8291 8487 8663 8711 8882 9080 Size: 33 54 12 196 176 48 171 198 Cuts at: 9080 9122 9491 9539 9557 9593 9803 9878 10058 Size: 42 369 48 18 36 210 75 180 Cuts at: 10058 10091 10160 10232 10679 11084 11626 11768 12137 Size: 33 69 72 447 405 542 142 369 Cuts at: 12137 12391 13811 14390 15320 15508 15517 15775 16910 Size: 254 1420 579 930 188 9 258 1135 Cuts at: 16910 17591 17782 826 Size: 681 191 1471 Fragments arranged by size: 1471 1420 1339 1135 930 875 681 590 579 542 531 507 447 405 372 369 369 303 279 258 254 242 224 222 219 211 210 198 196 191 188 180 180 180 176 171 150 142 111 105 99 87 75 75 75 72 69 69 54 54 54 51 48 48 48 42 36 33 33 25 25 24 24 18 16 12 9 SfiI GGCCn_nnn'nGGCC Cuts at: 4445 4685 5441 5471 5981 6692 7490 8207 8738 Size: 240 756 30 510 711 798 717 531 Cuts at: 8738 11346 15146 15200 4445 Size: 2608 3800 54 7672 Fragments arranged by size: 7672 3800 2608 798 756 717 711 531 510 240 54 30 SfoI GGC'GCC Cuts at: 3626 5746 6737 8033 8153 14687 15373 3626 Size: 2120 991 1296 120 6534 686 6680 Fragments arranged by size: 6680 6534 2120 1296 991 686 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 18427 SgrAI Cr'CCGG_yG Cuts at: 1700 13502 14804 15062 1700 Size: 11802 1302 258 5065 Fragments arranged by size: 11802 5065 1302 258 SmaI CCC'GGG Cuts at: 1872 2354 3845 8375 8468 10790 11465 15578 15754 Size: 482 1491 4530 93 2322 675 4113 176 Cuts at: 15754 1872 Size: 4545 Fragments arranged by size: 4545 4530 4113 2322 1491 675 482 176 93 SmlI C'TyrA_G Cuts at: 820 1017 1943 3149 3461 5348 5420 5459 5639 Size: 197 926 1206 312 1887 72 39 180 Cuts at: 5639 5690 5963 6992 7628 7652 8813 9020 9059 Size: 51 273 1029 636 24 1161 207 39 Cuts at: 9059 9239 9497 12077 13064 13115 13214 15410 16531 Size: 180 258 2580 987 51 99 2196 1121 Cuts at: 16531 17432 17694 820 Size: 901 262 1553 Fragments arranged by size: 2580 2196 1887 1553 1206 1161 1121 1029 987 926 901 636 312 273 262 258 207 197 180 180 99 72 51 51 39 39 24 SnaBI TAC'GTA Cuts at: 493 16218 493 Size: 15725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 18427 SphI G_CATG'C Cuts at: 1132 6277 15785 1132 Size: 5145 9508 3774 Fragments arranged by size: 9508 5145 3774 SrfI GCCC'GGGC Cuts at: 8468 8468 Size: 18427 SspI AAT'ATT Cuts at: 5 52 16351 5 Size: 47 16299 2081 Fragments arranged by size: 16299 2081 47 StuI AGG'CCT Cuts at: 1517 2750 3765 4434 4889 5078 5474 7706 11624 Size: 1233 1015 669 455 189 396 2232 3918 Cuts at: 11624 14555 15032 15149 1517 Size: 2931 477 117 4795 Fragments arranged by size: 4795 3918 2931 2232 1233 1015 669 477 455 396 189 117 StyI C'CwwG_G Cuts at: 513 1065 2101 2485 2565 3078 3102 3909 4449 Size: 552 1036 384 80 513 24 807 540 Cuts at: 4449 5469 5739 5994 6390 7359 8133 9003 9660 Size: 1020 270 255 396 969 774 870 657 Cuts at: 9660 10635 10752 11606 11619 12180 12612 12927 13764 Size: 975 117 854 13 561 432 315 837 Cuts at: 13764 13914 14197 14346 15498 15866 513 Size: 150 283 149 1152 368 3074 Fragments arranged by size: 3074 1152 1036 1020 975 969 870 854 837 807 774 657 561 552 540 513 432 396 384 368 315 283 270 255 150 149 117 80 24 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3667 4494 5684 8693 9371 11804 14342 Size: 1255 2087 827 1190 3009 678 2433 2538 Cuts at: 14342 16454 16471 16624 16809 17254 325 Size: 2112 17 153 185 445 1498 Fragments arranged by size: 3009 2538 2433 2112 2087 1498 1255 1190 827 678 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3546 4413 5907 Size: 274 80 33 51 1443 1569 867 1494 Cuts at: 5907 6081 11865 12756 16673 18385 96 Size: 174 5784 891 3917 1712 138 Fragments arranged by size: 5784 3917 1712 1569 1494 1443 891 867 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3176 3208 3959 3991 9375 9407 12815 12847 13090 Size: 32 751 32 5384 32 3408 32 243 Cuts at: 13090 13122 14600 14632 18387 18419 3176 Size: 32 1478 32 3755 32 3184 Fragments arranged by size: 5384 3755 3408 3184 1478 751 243 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 15089 1316 Size: 13773 4654 XbaI T'CTAG_A Cuts at: 15763 15763 Size: 18427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3289 7447 9445 12034 12274 13771 14881 3289 Size: 4158 1998 2589 240 1497 1110 6835 Fragments arranged by size: 6835 4158 2589 1998 1497 1110 240 XhoI C'TCGA_G Cuts at: 1943 5459 5690 13064 13115 1943 Size: 3516 231 7374 51 7255 Fragments arranged by size: 7374 7255 3516 231 51 XmaI C'CCGG_G Cuts at: 1870 2352 3843 8373 8466 10788 11463 15576 15752 Size: 482 1491 4530 93 2322 675 4113 176 Cuts at: 15752 1870 Size: 4545 Fragments arranged by size: 4545 4530 4113 2322 1491 675 482 176 93 XmnI GAAnn'nnTTC Cuts at: 2225 15149 16556 2225 Size: 12924 1407 4096 Fragments arranged by size: 12924 4096 1407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10795 14475 16235 276 Size: 53 83 186 936 9261 3680 1760 2468 Fragments arranged by size: 9261 3680 2468 1760 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I FspAI NruI NsiI PacI RsrII SwaI