################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I AspEI AspI AvaI AvaII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI HaeII HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI Ksp632I KspI MamI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 234 GGTACC G^GTACC AfaI 235 GTAC GT^AC AflIII 180 ACACGT A^C[GA][CT]GT AlwI 129 GGATC GGATC(4/5) AseI 108 ATTAAT AT^TAAT AsnI 108 ATTAAT AT^TAAT Asp718I 234 GGTACC G^GTACC AspHI 256 GAGCTC G[AT]GC[AT]^C AviII 27 TGCGCA TGC^GCA BanI 234 GGTACC G^G[CT][GA]CC BbvI 40 GCAGC GCAGC(8/12) BfaI 175 CTAG C^TAG BlpI 286 GCTCAGC GC^T[ACGT]AGC BpmI 68 CTGGAG CTGGAG(16/14) Bpu1102I 286 GCTCAGC GC^T[ACGT]AGC BsaJI 230 CCCTGG C^C[ACGT][ACGT]GG BsiHKAI 256 GAGCTC G[AT]GC[AT]^C BsrI 67 ACTGG ACTGG(1/-1) BssKI 231 CCTGG ^CC[ACGT]GG BstNI 231 CCTGG CC^[AT]GG CelII 286 GCTCAGC GC^T[ACGT]AGC CfoI 28 GCGC GCG^C DpnII 130 GATC ^GATC EcoRII 231 CCTGG ^CC[AT]GG Fnu4HI 40 GCAGC GC^[ACGT]GC FspI 27 TGCGCA TGC^GCA HhaI 28 GCGC GCG^C HinP1I 28 GCGC G^CGC ItaI 40 GCAGC GC^[ACGT]GC KpnI 234 GGTACC GGTAC^C MaeI 175 CTAG C^TAG MaeII 182 ACGT A^CGT MaeIII 184 GTTAC ^GT[ACGT]AC MboI 130 GATC ^GATC MvaI 231 CCTGG CC^[AT]GG MwoI 286 GCTCAGCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 130 GATC ^GATC NlaIII 292 CATG CATG^ NlaIV 234 GGTACC GG[ACGT]^[ACGT]CC NspI 291 GCATGC [GA]CATG^[CT] PshBI 108 ATTAAT AT^TAAT RsaI 235 GTAC GT^AC SacI 256 GAGCTC GAGCT^C Sau3AI 130 GATC ^GATC ScrFI 231 CCTGG CC^[ACGT]GG SfaNI 25 GATGC GCATC(5/9) SphI 291 GCATGC GCATG^C TseI 40 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AluI 102 AGCT AG^CT 257 AGCT BanII 227 GAGCCC G[GA]GC[CT]^C 256 GAGCTC BmyI 227 GAGCCC G[AGT]GC[ACT]^C 256 GAGCTC Bsp1286I 227 GAGCCC G[AGT]GC[ACT]^C 256 GAGCTC Cac8I 291 GCATGC GC[ACGT]^[ACGT]GC 295 GCCAGC DdeI 97 CTGAG C^T[ACGT]AG 287 CTCAG MnlI 84 CCTC CCTC(7/6) 154 GAGG TspRI 145 CACTG CA[GC]TG[ACGT][ACGT]^ 243 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### MseI Tru9I Tsp509I