################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccI AccII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI AviII BamHI BciVI BclI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspDI BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KpnI KspI MamI MfeI MluI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AclI 1373 AACGTT AA^CGTT AflII 1974 CTTAAG C^TTAAG AhdI 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 824 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 2571 GGACC G^G[AT]CC BalI 1188 TGGCCA TGG^CCA BbrPI 1502 CACGTG CAC^GTG BbvCI 1276 GCTGAGG CCTCAGC(-5/-2) BcgI 2187 GCATTTCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1974 CTTAAG C^TTAAG BsgI 1246 GTGCAG GTGCAG(16/14) BsiEI 2365 CGGCCG CG[GA][CT]^CG BsmBI 2025 GAGACG CGTCTC(1/5) BsmI 2124 GCATTC GAATGC(1/-1) BspHI 564 TCATGA T^CATGA BspLU11I 1152 ACATGT A^CATGT BsrBI 1016 CCGCTC CCGCTC(-3/-3) BsrDI 1098 CATTGC GCAATG(2/0) BstAPI 157 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 791 TTCGAA TT^CGAA BstEII 1513 GGTGACC G^GT[ACGT]ACC BstPI 1513 GGTGACC G^GT[ACGT]ACC EagI 2365 CGGCCG C^GGCCG Eam1105I 310 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2365 CGGCCG C^GGCCG Eco52I 2365 CGGCCG C^GGCCG EcoO65I 1513 GGTGACC G^GT[ACGT]ACC HgaI 282 GCGTC GACGC(5/10) HpaI 2991 GTTAAC GTT^AAC MluNI 1188 TGGCCA TGG^CCA MscI 1188 TGGCCA TGG^CCA NcoI 2835 CCATGG C^CATGG NotI 2364 GCGGCCGC GC^GGCCGC NspI 1152 ACATGT [GA]CATG^[CT] NspV 791 TTCGAA TT^CGAA PmaCI 1502 CACGTG CAC^GTG PmlI 1502 CACGTG CAC^GTG PpuMI 2570 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 1373 AACGTT AA^CGTT RcaI 564 TCATGA T^CATGA SacI 208 GAGCTC GAGCT^C SapI 368 GCTCTTC GCTCTTC(1/4) SfiI 2966 GGCCAGTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 791 TTCGAA TT^CGAA SwaI 1341 ATTTAAAT ATTT^AAAT XbaI 1075 TCTAGA T^CTAGA XmnI 824 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 287 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 1192 CAGAAACTG ApaI 2436 GGGCCC GGGCC^C 2974 GGGCCC AseI 376 ATTAAT AT^TAAT 775 ATTAAT AsnI 376 ATTAAT AT^TAAT 775 ATTAAT BglI 1647 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2967 GCCAGTTGGGC BglII 745 AGATCT A^GATCT 2952 AGATCT BsaAI 512 TACGTG [CT]AC^GT[GA] 1502 CACGTG DraI 1342 TTTAAA TTT^AAA 2800 TTTAAA DsaI 1916 CCACGG C^C[GA][CT]GG 2835 CCATGG EcoNI 85 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1866 CCTTGAGGAGG HincII 307 GTTGAC GT[CT]^[GA]AC 2991 GTTAAC HindII 307 GTTGAC GT[CT]^[GA]AC 2991 GTTAAC MslI 397 CATACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 562 CATCATGATG PflMI 286 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2671 CCAGAAGGTGG PshBI 376 ATTAAT AT^TAAT 775 ATTAAT TaqI 792 TCGA T^CGA 2866 TCGA TthHB8I 792 TCGA T^CGA 2866 TCGA Van91I 286 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2671 CCAGAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccIII AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaI AvrII BanI BanII BbeI BbsI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII