(Circular) (Six-base) MAPSORT of: pFN21AE5012.seq Check: 4547 from: 1 to: 7708 pFN21AE5012 7708 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5518 278 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5051 1937 Size: 3114 4594 Acc65I G'GTAC_C Cuts at: 5029 5029 Size: 7708 AclI AA'CG_TT Cuts at: 1563 3379 5835 6208 1563 Size: 1816 2456 373 3063 Fragments arranged by size: 3063 2456 1816 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2775 3116 3703 3724 3748 3772 3820 Size: 978 165 341 587 21 24 24 48 Cuts at: 3820 3916 3940 4049 4084 4132 4276 4420 4468 Size: 96 24 109 35 48 144 144 48 Cuts at: 4468 4516 5771 7155 7423 1632 Size: 48 1255 1384 268 1917 Fragments arranged by size: 1917 1384 1255 978 587 341 268 165 144 144 109 96 48 48 48 48 35 24 24 24 21 AflII C'TTAA_G Cuts at: 820 1017 3979 820 Size: 197 2962 4549 Fragments arranged by size: 4549 2962 197 AflIII A'CryG_T Cuts at: 1203 2518 2831 3157 6607 7584 1203 Size: 1315 313 326 3450 977 1327 Fragments arranged by size: 3450 1327 1315 977 326 313 AgeI A'CCGG_T Cuts at: 6539 6539 Size: 7708 AhdI GACnn_n'nnGTC Cuts at: 2320 6437 2320 Size: 4117 3591 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7708 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 3686 3718 2844 Size: 32 810 32 6834 Fragments arranged by size: 6834 810 32 32 AlwNI CAG_nnn'CTG Cuts at: 2297 3202 5451 7023 2297 Size: 905 2249 1572 2982 Fragments arranged by size: 2982 2249 1572 905 ApaI G_GGCC'C Cuts at: 4445 4983 4445 Size: 538 7170 ApaLI G'TGCA_C Cuts at: 5765 6921 5765 Size: 1156 6552 ApoI r'AATT_y Cuts at: 1493 2603 2908 3220 3344 3460 4663 4824 5017 Size: 1110 305 312 124 116 1203 161 193 Cuts at: 5017 5243 5395 7504 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1203 1110 312 305 226 193 161 152 124 116 AseI AT'TA_AT Cuts at: 160 1032 2382 2781 6262 160 Size: 872 1350 399 3481 1606 Fragments arranged by size: 3481 1606 1350 872 399 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 AvaI C'yCGr_G Cuts at: 1870 1943 3944 4088 4398 5033 1870 Size: 73 2001 144 310 635 4545 Fragments arranged by size: 4545 2001 635 310 144 73 AvrII C'CTAG_G Cuts at: 3400 3749 3845 3400 Size: 349 96 7263 Fragments arranged by size: 7263 349 96 BamHI G'GATC_C Cuts at: 5038 5405 5038 Size: 367 7341 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3660 3792 3816 3840 4320 Size: 325 217 627 1873 132 24 24 480 Cuts at: 4320 4416 4464 5029 6484 618 Size: 96 48 565 1455 1842 Fragments arranged by size: 1873 1842 1455 627 565 480 325 217 132 96 48 24 24 BanII G_rGCy'C Cuts at: 1391 2217 4445 4493 4628 4983 5027 1391 Size: 826 2228 48 135 355 44 4072 Fragments arranged by size: 4072 2228 826 355 135 48 44 BbeI G_GCGC'C Cuts at: 3796 3820 4324 3796 Size: 24 504 7180 Fragments arranged by size: 7180 504 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2252 3678 3765 4437 4461 4557 928 Size: 971 353 1426 87 672 24 96 4079 Fragments arranged by size: 4079 1426 971 672 353 96 87 24 BbvCI CC'TCA_GC Cuts at: 3282 3282 Size: 7708 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4181 4215 5899 5933 1903 Size: 34 2244 34 1684 34 3678 Fragments arranged by size: 3678 2244 1684 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5600 6810 5600 Size: 1210 6498 BclI T'GATC_A Cuts at: 1548 1548 Size: 7708 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3658 4978 6319 Size: 107 122 71 631 605 1986 1320 1341 Cuts at: 6319 136 Size: 1525 Fragments arranged by size: 1986 1525 1341 1320 631 605 122 107 71 BglII A'GATC_T Cuts at: 2750 4957 7703 2750 Size: 2207 2746 2755 Fragments arranged by size: 2755 2746 2207 BlpI GC'TnA_GC Cuts at: 5125 5125 Size: 7708 Bme1580I G_kGCm'C Cuts at: 1790 4445 4983 5769 6925 1790 Size: 2655 538 786 1156 2573 Fragments arranged by size: 2655 2573 1156 786 538 BmgBI CAC'GTC Cuts at: 1318 2168 1318 Size: 850 6858 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2036 5439 6397 Size: 416 53 83 399 24 619 3403 958 Cuts at: 6397 442 Size: 1753 Fragments arranged by size: 3403 1753 958 619 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7708 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4200 4248 4200 Size: 48 7660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3651 3880 3904 3917 4048 4218 6368 1239 Size: 2412 229 24 13 131 170 2150 2579 Fragments arranged by size: 2579 2412 2150 229 170 131 24 13 Bpu10I CC'TnA_GC Cuts at: 3282 3282 Size: 7708 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3162 3625 3893 5833 6698 6996 3162 Size: 463 268 1940 865 298 3874 Fragments arranged by size: 3874 1940 865 463 298 268 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2811 3921 4219 4339 6371 882 Size: 617 1312 1110 298 120 2032 2219 Fragments arranged by size: 2219 2032 1312 1110 617 298 120 BsaAI yAC'GTr Cuts at: 493 1204 2519 3509 5499 493 Size: 711 1315 990 1990 2702 Fragments arranged by size: 2702 1990 1315 990 711 BsaBI GATnn'nnATC Cuts at: 7702 7702 Size: 7708 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2287 3793 3817 4321 Size: 53 83 186 936 754 1506 24 504 Cuts at: 4321 5515 5897 6532 275 Size: 1194 382 635 1451 Fragments arranged by size: 1506 1451 1194 936 754 635 504 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 3823 4173 4221 4341 4567 6141 6539 6813 6960 Size: 350 48 120 226 1574 398 274 147 Cuts at: 6960 7371 3823 Size: 411 4160 Fragments arranged by size: 4160 1574 411 398 350 274 226 147 120 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7483 Fragments arranged by size: 7483 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 3676 3794 3866 3877 3890 3914 3962 Size: 926 1564 118 72 11 13 24 48 Cuts at: 3962 3986 4034 4058 4130 4154 4586 1186 Size: 24 48 24 72 24 432 4308 Fragments arranged by size: 4308 1564 926 432 118 72 72 48 48 24 24 24 24 13 11 BseYI C'CCAG_C Cuts at: 2393 6911 7407 2393 Size: 4518 496 2694 Fragments arranged by size: 4518 2694 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3272 1163 Size: 573 1536 5599 Fragments arranged by size: 5599 1536 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4373 5158 5919 6947 1540 Size: 275 186 2372 785 761 1028 2301 Fragments arranged by size: 2372 2301 1028 785 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 4266 4290 4338 5769 5854 6925 2217 Size: 2049 24 48 1431 85 1071 3000 Fragments arranged by size: 3000 2049 1431 1071 85 48 24 BsmI GAATG_Cn' Cuts at: 4129 5219 5312 4129 Size: 1090 93 6525 Fragments arranged by size: 6525 1090 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4024 4024 Size: 7708 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 4266 4290 4338 4445 4493 4628 Size: 399 427 2049 24 48 107 48 135 Cuts at: 4628 4983 5027 5769 5854 6925 1391 Size: 355 44 742 85 1071 2174 Fragments arranged by size: 2174 2049 1071 742 427 399 355 135 107 85 48 48 44 24 BspEI T'CCGG_A Cuts at: 3823 4173 4221 4341 4567 7371 3823 Size: 350 48 120 226 2804 4160 Fragments arranged by size: 4160 2804 350 226 120 48 BspHI T'CATG_A Cuts at: 2569 5597 7327 2569 Size: 3028 1730 2950 Fragments arranged by size: 3028 2950 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5063 844 Size: 4219 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3023 5595 1394 Size: 429 1200 2572 3507 Fragments arranged by size: 3507 2572 1200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3102 6203 6377 65 Size: 1183 1854 3101 174 1396 Fragments arranged by size: 3101 1854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6352 6539 1700 Size: 110 4542 187 2869 Fragments arranged by size: 4542 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7708 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7708 BssSI C'ACGA_G Cuts at: 5768 6780 5768 Size: 1012 6696 BstAPI GCAn_nnn'nTGC Cuts at: 794 2168 794 Size: 1374 6334 BstBI TT'CG_AA Cuts at: 2797 6512 2797 Size: 3715 3993 BstEII G'GTnAC_C Cuts at: 1184 3518 1184 Size: 2334 5374 BstYI r'GATC_y Cuts at: 1972 2294 2750 4957 5038 5405 5790 5807 7259 Size: 322 456 2207 81 367 385 17 1452 Cuts at: 7259 7345 7357 7703 1972 Size: 86 12 346 1977 Fragments arranged by size: 2207 1977 1452 456 385 367 346 322 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 3969 4017 4137 4185 4377 4556 4725 3969 Size: 48 120 48 192 179 169 6952 Fragments arranged by size: 6952 192 179 169 120 48 48 BtgI C'CryG_G Cuts at: 513 1065 2004 3921 4840 513 Size: 552 939 1917 919 3381 Fragments arranged by size: 3381 1917 939 919 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6969 Fragments arranged by size: 6969 683 56 BtsI GCAGTG_nn' Cuts at: 5224 6018 6038 5224 Size: 794 20 6894 Fragments arranged by size: 6894 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7673 DraI TTT'AAA Cuts at: 3349 4807 5012 3349 Size: 1458 205 6045 Fragments arranged by size: 6045 1458 205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6715 809 Size: 507 5399 1802 Fragments arranged by size: 5399 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3193 3890 Size: 54 1425 50 275 18 745 618 697 Cuts at: 3890 4010 4202 4274 4298 4370 5155 6044 7673 Size: 120 192 72 24 72 785 889 1629 Cuts at: 7673 8 Size: 43 Fragments arranged by size: 1629 1425 889 785 745 697 618 275 192 120 72 72 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 4370 5155 1537 Size: 275 2558 785 4090 Fragments arranged by size: 4090 2558 785 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2275 2380 3116 4068 5638 1353 Size: 922 105 736 952 1570 3423 Fragments arranged by size: 3423 1570 952 922 736 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6295 6669 6815 387 Size: 1064 138 4706 374 146 1280 Fragments arranged by size: 4706 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 7708 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2610 2775 3116 3651 3703 3724 3748 Size: 393 978 165 341 535 52 21 24 Cuts at: 3748 3772 3820 3880 3904 3916 3917 3940 4048 Size: 24 48 60 24 12 1 23 108 Cuts at: 4048 4049 4084 4132 4218 4276 4420 4468 4516 Size: 1 35 48 86 58 144 48 48 Cuts at: 4516 5771 6368 7155 7423 1239 Size: 1255 597 787 268 1524 Fragments arranged by size: 1524 1255 978 787 597 535 393 341 268 165 144 108 86 60 58 52 48 48 48 48 35 24 24 24 23 21 12 1 1 EcoNI CCTnn'n_nnAGG Cuts at: 2094 3875 2094 Size: 1781 5927 EcoO109I rG'GnC_Cy Cuts at: 2048 3867 4441 4442 4576 4980 2048 Size: 1819 574 1 134 404 4776 Fragments arranged by size: 4776 1819 574 404 134 1 EcoRI G'AATT_C Cuts at: 5017 5395 5017 Size: 378 7330 FspI TGC'GCA Cuts at: 6214 6214 Size: 7708 HaeII r_GCGC'y Cuts at: 1678 3796 3820 4324 4396 6855 1678 Size: 2118 24 504 72 2459 2531 Fragments arranged by size: 2531 2459 2118 504 72 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2178 2210 2306 2338 4780 Size: 32 135 32 606 32 96 32 2442 Cuts at: 4780 4812 6349 6381 6423 6455 1373 Size: 32 1537 32 42 32 2626 Fragments arranged by size: 2626 2442 1537 606 135 96 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2314 4998 5052 5298 669 Size: 988 281 376 2684 54 246 3079 Fragments arranged by size: 3079 2684 988 376 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 7708 HpaI GTT'AAC Cuts at: 4998 5298 4998 Size: 300 7408 KasI G'GCGC_C Cuts at: 3792 3816 4320 3792 Size: 24 504 7180 Fragments arranged by size: 7180 504 24 KpnI G_GTAC'C Cuts at: 5033 5033 Size: 7708 MfeI C'AATT_G Cuts at: 5307 5307 Size: 7708 MluI A'CGCG_T Cuts at: 7584 7584 Size: 7708 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3703 6822 7006 1305 Size: 2398 3119 184 2007 Fragments arranged by size: 3119 2398 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3195 10 Size: 54 1425 343 1363 4523 Fragments arranged by size: 4523 1425 1363 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2406 2571 5666 6025 6184 518 Size: 648 1240 165 3095 359 159 2042 Fragments arranged by size: 3095 2042 1240 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 5124 5801 6949 7194 1606 Size: 3518 677 1148 245 2120 Fragments arranged by size: 3518 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3793 3817 4321 3793 Size: 24 504 7180 Fragments arranged by size: 7180 504 24 NcoI C'CATG_G Cuts at: 513 1065 2004 4840 513 Size: 552 939 2836 3381 Fragments arranged by size: 3381 2836 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7708 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7708 NotI GC'GGCC_GC Cuts at: 4370 5155 4370 Size: 785 6923 NspI r_CATG'y Cuts at: 1132 1229 3161 5066 6611 1132 Size: 97 1932 1905 1545 2229 Fragments arranged by size: 2229 1932 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7708 PciI A'CATG_T Cuts at: 3157 6607 3157 Size: 3450 4258 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 4682 1108 Size: 1189 2385 4134 Fragments arranged by size: 4134 2385 1189 PfoI T'CCnGG_A Cuts at: 2290 2290 Size: 7708 PmeI GTTT'AAAC Cuts at: 5012 5012 Size: 7708 PmlI CAC'GTG Cuts at: 3509 3509 Size: 7708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2821 2853 5777 5809 7290 7322 2821 Size: 32 2924 32 1481 32 3207 Fragments arranged by size: 3207 2924 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4576 4576 Size: 7708 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7708 PsiI TTA'TAA Cuts at: 5278 5278 Size: 7708 PspOMI G'GGCC_C Cuts at: 4441 4979 4441 Size: 538 7170 PstI C_TGCA'G Cuts at: 830 5060 830 Size: 4230 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7708 SacI G_AGCT'C Cuts at: 2217 2217 Size: 7708 SalI G'TCGA_C Cuts at: 1936 5050 1936 Size: 3114 4594 SapI GCTCTTCn'nnn_ Cuts at: 2380 2380 Size: 7708 SbfI CC_TGCA'GG Cuts at: 5060 5060 Size: 7708 ScaI AGT'ACT Cuts at: 5956 7668 5956 Size: 1712 5996 SfcI C'TryA_G Cuts at: 826 1045 2010 2208 2250 4899 5056 6191 6872 Size: 219 965 198 42 2649 157 1135 681 Cuts at: 6872 7063 826 Size: 191 1471 Fragments arranged by size: 2649 1471 1135 965 681 219 198 191 157 42 SfiI GGCCn_nnn'nGGCC Cuts at: 4978 4978 Size: 7708 SfoI GGC'GCC Cuts at: 3794 3818 4322 3794 Size: 24 504 7180 Fragments arranged by size: 7180 504 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7708 SmaI CCC'GGG Cuts at: 1872 5035 1872 Size: 3163 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3177 3604 3872 3979 5812 6713 Size: 197 926 1234 427 268 107 1833 901 Cuts at: 6713 6975 820 Size: 262 1553 Fragments arranged by size: 1833 1553 1234 926 901 427 268 262 197 107 SnaBI TAC'GTA Cuts at: 493 5499 493 Size: 5006 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7708 SphI G_CATG'C Cuts at: 1132 5066 1132 Size: 3934 3774 SspI AAT'ATT Cuts at: 5 52 5632 5 Size: 47 5580 2081 Fragments arranged by size: 5580 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7708 StyI C'CwwG_G Cuts at: 513 1065 2004 3400 3749 3797 3845 4840 5147 Size: 552 939 1396 349 48 48 995 307 Cuts at: 5147 513 Size: 3074 Fragments arranged by size: 3074 1396 995 939 552 349 307 48 48 SwaI ATTT'AAAT Cuts at: 3349 3349 Size: 7708 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4434 5735 5752 5905 6090 6535 325 Size: 1255 2854 1301 17 153 185 445 1498 Fragments arranged by size: 2854 1498 1301 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5954 7666 96 Size: 274 80 33 51 1443 3977 1712 138 Fragments arranged by size: 3977 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3968 4000 7668 7700 3968 Size: 32 3668 32 3976 Fragments arranged by size: 3976 3668 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7708 XbaI T'CTAG_A Cuts at: 3080 5044 3080 Size: 1964 5744 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7708 XmaI C'CCGG_G Cuts at: 1870 5033 1870 Size: 3163 4545 XmnI GAAnn'nnTTC Cuts at: 2833 5837 2833 Size: 3004 4704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5516 276 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SacII SanDI SexAI SrfI XcmI