Restriction map for
pFN21AE5434
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeII MamI
MfeI MluI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 845 TCCGGA T^CCGGA
BalI 570 TGGCCA TGG^CCA
BamHI 833 GGATCC G^GATCC
BbvI 414 GCTGC GCAGC(8/12)
BciVI 355 GGATAC GTATCC(6/5)
BsaI 480 GAGACC GGTCTC(1/5)
BsaWI 845 TCCGGA [AT]^CCGG[AT]
BseAI 845 TCCGGA T^CCGGA
BsmFI 292 GTCCC GGGAC(10/14)
Bsp1407I 858 TGTACA T^GTACA
BspEI 845 TCCGGA T^CCGGA
BsrDI 449 GCAATG GCAATG(2/0)
BsrGI 858 TGTACA T^GTACA
BsrI 241 ACTGG ACTGG(1/-1)
BssHII 68 GCGCGC G^CGCGC
BstF5I 840 GGATG GGATG(2/0)
BstYI 833 GGATCC [GA]^GATC[CT]
DraII 15 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 570 TGGCCA [CT]^GGCC[GA]
EarI 709 GAAGAG CTCTTC(1/4)
EcoO109I 15 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 89 CCTTGG C^C[AT][AT]GG
FauI 72 GCGGG CCCGC(4/6)
FokI 840 GGATG GGATG(9/13)
HaeII 554 AGCGCC [GA]GCGC^[CT]
Ksp632I 709 GAAGAG CTCTTC(1/4)
KspI 55 CCGCGG CCGC^GG
MflI 833 GGATCC [GA]^GATC[CT]
MluNI 570 TGGCCA TGG^CCA
MroI 845 TCCGGA T^CCGGA
MscI 570 TGGCCA TGG^CCA
NlaIV 833 GGATCC GG[ACGT]^[ACGT]CC
PvuII 412 CAGCTG CAG^CTG
SacII 55 CCGCGG CCGC^GG
SmlI 195 CTCAAG C^T[CT][GA]AG
SspBI 858 TGTACA T^GTACA
StyI 89 CCTTGG C^C[AT][AT]GG
TaqI 231 TCGA T^CGA
TseI 414 GCTGC G^C[AT]GC
Tsp45I 901 GTGAC ^GT[GC]AC
TthHB8I 231 TCGA T^CGA
XhoII 833 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanII 22 GAGCCC G[GA]GC[CT]^C
165 GAGCCC
BbsI 45 GTCTTC GAAGAC(2/6)
157 GTCTTC
BcnI 25 CCCGG CC^[GC]GG
50 CCGGG
BfaI 623 CTAG C^TAG
949 CTAG
BmyI 22 GAGCCC G[AGT]GC[ACT]^C
165 GAGCCC
BpuAI 45 GTCTTC GAAGAC(2/6)
157 GTCTTC
BsmAI 480 GAGAC GTCTC(1/5)
864 GTCTC
Bsp1286I 22 GAGCCC G[AGT]GC[ACT]^C
165 GAGCCC
BstNI 543 CCTGG CC^[AT]GG
837 CCTGG
Cac8I 29 GCGAGC GC[ACGT]^[ACGT]GC
68 GCGCGC
Cfr13I 16 GGCCC G^G[ACGT]CC
52 GGGCC
DdeI 20 CTGAG C^T[ACGT]AG
335 CTAAG
DsaI 55 CCGCGG C^C[GA][CT]GG
169 CCACGG
EcoRII 543 CCTGG ^CC[AT]GG
837 CCTGG
HphI 114 GGTGA GGTGA(8/7)
322 GGTGA
MaeI 623 CTAG C^TAG
949 CTAG
MaeIII 901 GTGAC ^GT[ACGT]AC
975 GTTAC
MspA1I 55 CCGCGG C[AC]G^C[GT]G
412 CAGCTG
MvaI 543 CCTGG CC^[AT]GG
837 CCTGG
NciI 25 CCCGG CC^[GC]GG
50 CCGGG
PleI 517 GAGTC GAGTC(4/5)
798 GAGTC
Sau96I 16 GGCCC G^G[ACGT]CC
52 GGGCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BpmI BsaJI BseRI
BsiYI BslI BssKI BstUI CfoI
DpnII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MseI MspI MvnI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI TfiI Tru9I Tsp509I TspRI