Restriction map for
pFN21AE5434
(Circular) (Six-base) MAPSORT of: pFN21AE5434.seq Check: 4724 from: 1 to: 5698
pFN21AE5434 5698 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 2540 3761 5145 5413 1632
Size: 675 233 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 675 268 233
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2584 2778 2879 3007 3233 3385 5494 1493
Size: 1091 194 101 128 226 152 2109 1697
Fragments arranged by size:
2109 1697 1091 226 194 152 128 101
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 2838 3028 3395 2838
Size: 190 367 5141
Fragments arranged by size:
5141 367 190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2174 3017 1391
Size: 640 143 843 4072
Fragments arranged by size:
4072 843 640 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2155 928
Size: 971 144 112 4471
Fragments arranged by size:
4471 971 144 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2354 3590 4800 2354
Size: 1236 1210 3252
Fragments arranged by size:
3252 1236 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 3429 4387
Size: 416 53 83 399 24 839 1173 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1173 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2570 2694 2969 4358 1239
Size: 1301 30 124 275 1389 2579
Fragments arranged by size:
2579 1389 1301 275 124 30
Bpu10I CC'TnA_GC
Cuts at: 2025 2025
Size: 5698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 3823 4688 4986 2185
Size: 1638 865 298 2897
Fragments arranged by size:
2897 1638 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2479 4361 882
Size: 617 980 1882 2219
Fragments arranged by size:
2219 1882 980 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2850 4131 4529 4803 4950 5361 2850
Size: 1281 398 274 147 411 3187
Fragments arranged by size:
3187 1281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2443 2473 1178
Size: 30 165 30 630 30 380 30 4403
Fragments arranged by size:
4403 630 380 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2550 2732 1186
Size: 873 491 182 4152
Fragments arranged by size:
4152 873 491 182
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3759 3844 4915 3759
Size: 85 1071 4542
Fragments arranged by size:
4542 1071 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2174 3017 3759 3844 4915 1391
Size: 399 241 143 843 742 85 1071 2174
Fragments arranged by size:
2174 1071 843 742 399 241 143 85
BspEI T'CCGG_A
Cuts at: 2850 5361 2850
Size: 2511 3187
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 4193 4367 65
Size: 1183 1213 1732 174 1396
Fragments arranged by size:
1732 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 2863 96
Size: 2767 2931
BssHII G'CGCG_C
Cuts at: 1925 2073 1925
Size: 148 5550
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2838 3028 3395 3780 3797 5249 5335 5347
Size: 866 190 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 866 385 367 346 190 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2060 2174 513
Size: 552 939 56 114 4037
Fragments arranged by size:
4037 939 552 114 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2948 3214 4008 4028 2948
Size: 266 794 20 4618
Fragments arranged by size:
4618 794 266 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3145 4034
Size: 54 1425 50 275 18 745 570 889
Cuts at: 4034 5663 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 570 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2709 3628 1353
Size: 1356 919 3423
Fragments arranged by size:
3423 1356 919
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2031 4285 4659 4805 387
Size: 1064 138 435 7 2254 374 146 1280
Fragments arranged by size:
2254 1280 1064 435 374 146 138 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2540 2570 2694 2969 3761 4358
Size: 393 675 233 30 124 275 792 597
Cuts at: 4358 5145 5413 1239
Size: 787 268 1524
Fragments arranged by size:
1524 792 787 675 597 393 275 268
233 124 30
EcoO109I rG'GnC_Cy
Cuts at: 2021 2021
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2563 4845 1678
Size: 885 2282 2531
Fragments arranged by size:
2531 2282 885
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2170 2202 2321
Size: 32 135 32 461 32 105 32 119
Cuts at: 2321 2353 2711 2743 4339 4371 4413 4445 1373
Size: 32 358 32 1596 32 42 32 2626
Fragments arranged by size:
2626 1596 461 358 135 119 105 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2577 10
Size: 54 1425 343 745 3131
Fragments arranged by size:
3131 1425 745 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2419 3114 3791 4939 5184 1606
Size: 456 357 695 677 1148 245 2120
Fragments arranged by size:
2120 1148 695 677 456 357 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2419 2419
Size: 5698
SacII CC_GC'GG
Cuts at: 2063 2063
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 3046 4181 4862 5053 826
Size: 219 2001 1135 681 191 1471
Fragments arranged by size:
2001 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 3802 4703 4965 820
Size: 197 926 257 1602 901 262 1553
Fragments arranged by size:
1602 1553 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 3137 513
Size: 552 939 90 1043 3074
Fragments arranged by size:
3074 1043 939 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2696 2828 2863
Size: 274 80 33 51 1443 719 132 35
Cuts at: 2863 3944 5656 96
Size: 1081 1712 138
Fragments arranged by size:
1712 1443 1081 719 274 138 132 80
51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2823 2855 5658 5690 2823
Size: 32 2803 32 2831
Fragments arranged by size:
2831 2803 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI