################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII HphI KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 117 AAATTT [GA]^AATT[CT] ApoI 117 AAATTT [GA]^AATT[CT] AspHI 230 GAGCTC G[AT]GC[AT]^C AvaII 77 GGTCC G^G[AT]CC BanII 230 GAGCTC G[GA]GC[CT]^C BbvI 59 GCTGC GCAGC(8/12) BfaI 22 CTAG C^TAG BmyI 230 GAGCTC G[AGT]GC[ACT]^C BsaI 218 GAGACC GGTCTC(1/5) BsaJI 93 CCCTGG C^C[ACGT][ACGT]GG BsiHKAI 230 GAGCTC G[AT]GC[AT]^C BsmAI 218 GAGAC GTCTC(1/5) BsmI 184 GAATGC GAATGC(1/-1) Bsp1286I 230 GAGCTC G[AGT]GC[ACT]^C BssKI 94 CCTGG ^CC[ACGT]GG BstBI 181 TTCGAA TT^CGAA BstNI 94 CCTGG CC^[AT]GG CfoI 90 GCGC GCG^C Cfr13I 77 GGTCC G^G[ACGT]CC EarI 250 GAAGAG CTCTTC(1/4) EcoRII 94 CCTGG ^CC[AT]GG Fnu4HI 59 GCTGC GC^[ACGT]GC HaeIII 28 GGCC GG^CC HhaI 90 GCGC GCG^C HinP1I 90 GCGC G^CGC HincII 158 GTCAAC GT[CT]^[GA]AC HindII 158 GTCAAC GT[CT]^[GA]AC HinfI 127 GATTC G^A[ACGT]TC ItaI 59 GCTGC GC^[ACGT]GC Ksp632I 250 GAAGAG CTCTTC(1/4) MaeI 22 CTAG C^TAG MaeII 204 ACGT A^CGT MvaI 94 CCTGG CC^[AT]GG NspV 181 TTCGAA TT^CGAA SacI 230 GAGCTC GAGCT^C Sau96I 77 GGTCC G^G[ACGT]CC ScrFI 94 CCTGG CC^[ACGT]GG SfaNI 109 GATGC GCATC(5/9) SfuI 181 TTCGAA TT^CGAA TaqI 182 TCGA T^CGA TfiI 127 GATTC G^A[AT]TC TseI 59 GCTGC G^C[AT]GC TspRI 73 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 182 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AluI 24 AGCT AG^CT 231 AGCT BpmI 63 CTCCAG CTGGAG(16/14) 95 CTGGAG Cac8I 25 GCTGGC GC[ACGT]^[ACGT]GC 68 GCCAGC Tsp509I 9 AATT ^AATT 118 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BsrI MboII MnlI MwoI