################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 664 GCGG CCGC(-3/-1) AcyI 358 GGCGCC G[GA]^CG[CT]C AluI 672 AGCT AG^CT Asp700I 543 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 285 GGATCC G^GATCC BanI 358 GGCGCC G^G[CT][GA]CC BanII 643 GAGCCC G[GA]GC[CT]^C BbeI 358 GGCGCC GGCGC^C BfaI 299 CTAG C^TAG BsaHI 358 GGCGCC G[GA]^CG[CT]C BsaI 43 GGTCTC GGTCTC(1/5) BsmBI 65 GAGACG CGTCTC(1/5) BsrFI 423 ACCGGC [GA]^CCGG[CT] BstYI 285 GGATCC [GA]^GATC[CT] Cfr10I 423 ACCGGC [GA]^CCGG[CT] DraIII 428 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 251 CCGTGG C^C[GA][CT]GG EcoNI 492 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 349 GATATC GAT^ATC EcoT14I 497 CCAAGG C^C[AT][AT]GG HaeII 358 GGCGCC [GA]GCGC^[CT] Hin1I 358 GGCGCC G[GA]^CG[CT]C KasI 358 GGCGCC G^GCGCC MaeI 299 CTAG C^TAG MflI 285 GGATCC [GA]^GATC[CT] MslI 48 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 358 GGCGCC GG^CGCC PaeR7I 512 CTCGAG C^TCGAG PleI 604 GACTC GAGTC(4/5) SfaNI 292 GATGC GCATC(5/9) SfcI 198 CTACAG C^T[GA][CT]AG SfoI 358 GGCGCC GGC^GCC SmaI 243 CCCGGG CCC^GGG SmlI 512 CTCGAG C^T[CT][GA]AG StyI 497 CCAAGG C^C[AT][AT]GG TaqI 513 TCGA T^CGA TthHB8I 513 TCGA T^CGA XhoI 512 CTCGAG C^TCGAG XhoII 285 GGATCC [GA]^GATC[CT] XmaCI 243 CCCGGG C^CCGGG XmaI 243 CCCGGG C^CCGGG XmnI 543 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 286 GATCC GGATC(4/5) 285 GGATC AvaI 243 CCCGGG C^[CT]CG[GA]G 512 CTCGAG BcnI 243 CCCGG CC^[GC]GG 244 CCGGG BglI 91 GCCCATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 403 GCCTACCAGGC BmyI 89 GTGCCC G[AGT]GC[ACT]^C 643 GAGCCC BsoBI 243 CCCGGG C^[CT]CG[GA]G 512 CTCGAG Bsp1286I 89 GTGCCC G[AGT]GC[ACT]^C 643 GAGCCC BspHI 47 TCATGA T^CATGA 584 TCATGA BstF5I 72 GGATG GGATG(2/0) 221 GGATG CfoI 260 GCGC GCG^C 359 GCGC DpnII 210 GATC ^GATC 286 GATC DraII 98 AGGCCCT [GA]G^G[ACGT]CC[CT] 131 AGGGCCT EcoO109I 98 AGGCCCT [GA]G^G[ACGT]CC[CT] 131 AGGGCCT FokI 72 GGATG GGATG(9/13) 221 GGATG HapII 244 CCGG C^CGG 424 CCGG HhaI 260 GCGC GCG^C 359 GCGC HinP1I 260 GCGC G^CGC 359 GCGC HpaII 244 CCGG C^CGG 424 CCGG MboI 210 GATC ^GATC 286 GATC MspI 244 CCGG C^CGG 424 CCGG NciI 243 CCCGG CC^[GC]GG 244 CCGGG NdeII 210 GATC ^GATC 286 GATC RcaI 47 TCATGA T^CATGA 584 TCATGA Sau3AI 210 GATC ^GATC 286 GATC TfiI 323 GATTC G^A[AT]TC 381 GATTC Tsp45I 22 GTCAC ^GT[GC]AC 58 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### BbvI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI TspRI