(Circular) (Six-base) MAPSORT of: pFN21AE1720.seq Check: 5511 from: 1 to: 5386 pFN21AE1720 5386 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3196 278 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 1937 Size: 792 4594 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2393 2456 2592 3449 4833 5101 1632 Size: 761 63 136 857 1384 268 1917 Fragments arranged by size: 1917 1384 857 761 268 136 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 4285 5262 1203 Size: 3082 977 1327 Fragments arranged by size: 3082 1327 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5386 AlwNI CAG_nnn'CTG Cuts at: 3129 4701 3129 Size: 1572 3814 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2695 2921 3073 5182 1493 Size: 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 3940 160 Size: 872 2908 1606 Fragments arranged by size: 2908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2248 2517 2711 1870 Size: 73 305 269 194 4545 Fragments arranged by size: 4545 305 269 194 73 BamHI G'GATC_C Cuts at: 2290 2716 3083 2290 Size: 426 367 4593 Fragments arranged by size: 4593 426 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2363 2707 4162 618 Size: 325 217 627 576 344 1455 1842 Fragments arranged by size: 1842 1455 627 576 344 325 217 BanII G_rGCy'C Cuts at: 1391 2652 2705 1391 Size: 1261 53 4072 Fragments arranged by size: 4072 1261 53 BbeI G_GCGC'C Cuts at: 2367 2367 Size: 5386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3577 3611 1903 Size: 34 1640 34 3678 Fragments arranged by size: 3678 1640 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3278 4488 3278 Size: 1210 4176 BclI T'GATC_A Cuts at: 1548 1548 Size: 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2414 3997 Size: 107 122 71 631 605 430 312 1583 Cuts at: 3997 136 Size: 1525 Fragments arranged by size: 1583 1525 631 605 430 312 122 107 71 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 2098 3447 4603 1790 Size: 308 1349 1156 2573 Fragments arranged by size: 2573 1349 1156 308 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5386 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2054 2578 3117 Size: 416 53 83 399 24 637 524 539 Cuts at: 3117 4075 442 Size: 958 1753 Fragments arranged by size: 1753 958 637 539 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 2353 2606 4046 1239 Size: 849 265 253 1440 2579 Fragments arranged by size: 2579 1440 849 265 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3511 4376 4674 3511 Size: 865 298 4223 Fragments arranged by size: 4223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 4049 882 Size: 617 555 1995 2219 Fragments arranged by size: 2219 1995 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3177 493 Size: 711 1973 2702 Fragments arranged by size: 2702 1973 711 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2364 3193 3575 4210 Size: 53 83 186 936 831 829 382 635 Cuts at: 4210 275 Size: 1451 Fragments arranged by size: 1451 936 831 829 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3819 4217 4491 4638 5049 3819 Size: 398 274 147 411 4156 Fragments arranged by size: 4156 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2604 2634 1178 Size: 30 165 30 1201 30 3930 Fragments arranged by size: 3930 1201 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5386 BseYI C'CCAG_C Cuts at: 4589 5085 4589 Size: 496 4890 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4813 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 3597 4625 1540 Size: 275 186 835 761 1028 2301 Fragments arranged by size: 2301 1028 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3447 3532 4603 3447 Size: 85 1071 4230 Fragments arranged by size: 4230 1071 85 BsmI GAATG_Cn' Cuts at: 2897 2990 2897 Size: 93 5293 BsmBI CGTCTCn'nnnn_ Cuts at: 2064 2064 Size: 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2652 2705 3447 3532 4603 1391 Size: 399 308 554 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 308 85 53 BspEI T'CCGG_A Cuts at: 5049 5049 Size: 5386 BspHI T'CATG_A Cuts at: 2052 2589 3275 5005 2052 Size: 537 686 1730 2433 Fragments arranged by size: 2433 1730 686 537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2741 844 Size: 1897 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3273 1394 Size: 429 1450 3507 Fragments arranged by size: 3507 1450 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3881 4055 65 Size: 1183 2633 174 1396 Fragments arranged by size: 2633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2428 4030 4217 1700 Size: 110 618 1602 187 2869 Fragments arranged by size: 2869 1602 618 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5386 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2290 2716 3083 3468 3485 4937 5023 5035 Size: 318 426 367 385 17 1452 86 12 Cuts at: 5035 5381 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 3643 Fragments arranged by size: 3643 939 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2432 487 Size: 683 56 1206 3441 Fragments arranged by size: 3441 1206 683 56 BtsI GCAGTG_nn' Cuts at: 2902 3696 3716 2902 Size: 794 20 4572 Fragments arranged by size: 4572 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5351 DraI TTT'AAA Cuts at: 2690 2690 Size: 5386 DraIII CAC_nnn'GTG Cuts at: 2438 2438 Size: 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4393 809 Size: 507 3077 1802 Fragments arranged by size: 3077 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2833 3722 5351 Size: 54 1425 50 275 18 1003 889 1629 Cuts at: 5351 8 Size: 43 Fragments arranged by size: 1629 1425 1003 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 1537 Size: 275 1021 4090 Fragments arranged by size: 4090 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3316 1353 Size: 1963 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2684 3973 4347 4493 387 Size: 1064 138 1095 1289 374 146 1280 Fragments arranged by size: 1289 1280 1095 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2353 2393 2456 2592 2606 3449 Size: 393 456 265 40 63 136 14 843 Cuts at: 3449 4046 4833 5101 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 843 787 597 456 393 268 265 136 63 40 14 EcoNI CCTnn'n_nnAGG Cuts at: 2501 2501 Size: 5386 EcoO109I rG'GnC_Cy Cuts at: 2104 2137 2104 Size: 33 5353 EcoRI G'AATT_C Cuts at: 2695 3073 2695 Size: 378 5008 EcoRV GAT'ATC Cuts at: 2356 2356 Size: 5386 FspI TGC'GCA Cuts at: 3892 3892 Size: 5386 HaeII r_GCGC'y Cuts at: 1678 2367 4533 1678 Size: 689 2166 2531 Fragments arranged by size: 2531 2166 689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2199 2231 2275 2307 4027 Size: 32 135 32 627 32 44 32 1720 Cuts at: 4027 4059 4101 4133 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1720 627 135 44 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2730 2976 669 Size: 988 281 792 246 3079 Fragments arranged by size: 3079 988 792 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5386 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KasI G'GCGC_C Cuts at: 2363 2363 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1991 4500 4684 1305 Size: 686 2509 184 2007 Fragments arranged by size: 2509 2007 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3564 Fragments arranged by size: 3564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3344 3703 3862 518 Size: 648 891 1287 359 159 2042 Fragments arranged by size: 2042 1287 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2802 3479 4627 4872 1606 Size: 1196 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 677 245 NarI GG'CG_CC Cuts at: 2364 2364 Size: 5386 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3895 Fragments arranged by size: 3895 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2744 4289 1132 Size: 97 1515 1545 2229 Fragments arranged by size: 2229 1545 1515 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5386 PciI A'CATG_T Cuts at: 4285 4285 Size: 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4484 PfoI T'CCnGG_A Cuts at: 2222 2222 Size: 5386 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 4968 5000 3455 Size: 32 1481 32 3841 Fragments arranged by size: 3841 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PspXI vC'TCGA_Gb Cuts at: 2517 2517 Size: 5386 PstI C_TGCA'G Cuts at: 830 2738 830 Size: 1908 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2728 1936 Size: 792 4594 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SfcI C'TryA_G Cuts at: 826 1045 2203 2734 3869 4550 4741 826 Size: 219 1158 531 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 681 531 219 191 SfoI GGC'GCC Cuts at: 2365 2365 Size: 5386 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2250 2713 1872 Size: 378 463 4545 Fragments arranged by size: 4545 463 378 SmlI C'TyrA_G Cuts at: 820 1017 1943 2517 3490 4391 4653 820 Size: 197 926 574 973 901 262 1553 Fragments arranged by size: 1553 973 926 901 574 262 197 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2744 1132 Size: 1612 3774 SspI AAT'ATT Cuts at: 5 52 3310 5 Size: 47 3258 2081 Fragments arranged by size: 3258 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2004 2502 2825 513 Size: 552 939 498 323 3074 Fragments arranged by size: 3074 939 552 498 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 3413 3430 3583 3768 4213 325 Size: 1255 1017 816 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1017 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3632 5344 96 Size: 274 80 33 51 1443 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2604 2636 5346 5378 2604 Size: 32 2710 32 2612 Fragments arranged by size: 2710 2612 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XhoI C'TCGA_G Cuts at: 1943 2517 1943 Size: 574 4812 XmaI C'CCGG_G Cuts at: 1870 2248 2711 1870 Size: 378 463 4545 Fragments arranged by size: 4545 463 378 XmnI GAAnn'nnTTC Cuts at: 2552 3515 2552 Size: 963 4423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3194 276 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI