################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AhdI Alw44I AlwI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tru9I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 835 GAATTT [GA]^AATT[CT] AgeI 744 ACCGGT A^CCGGT Aor51HI 869 AGCGCT AGC^GCT ApoI 835 GAATTT [GA]^AATT[CT] AspHI 358 GAGCTC G[AT]GC[AT]^C AvaII 691 GGACC G^G[AT]CC BbeI 788 GGCGCC GGCGC^C BbvCI 730 GCTGAGG CCTCAGC(-5/-2) BfaI 226 CTAG C^TAG BglI 150 GCCCTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 917 AGATCT A^GATCT BsaI 590 GAGACC GGTCTC(1/5) BsaWI 744 ACCGGT [AT]^CCGG[AT] BsgI 907 GTGCAG GTGCAG(16/14) BsiHKAI 358 GAGCTC G[AT]GC[AT]^C BspMI 909 GCAGGT ACCTGC(4/8) BsrBI 160 CCGCTC CCGCTC(-3/-3) BsrI 724 ACTGG ACTGG(1/-1) BssSI 163 CTCGTG CACGAG(-5/-1) BstF5I 642 GGATG GGATG(2/0) BstYI 917 AGATCT [GA]^GATC[CT] Eco47III 869 AGCGCT AGC^GCT FokI 642 GGATG GGATG(9/13) HincII 1033 GTCAAC GT[CT]^[GA]AC HindII 1033 GTCAAC GT[CT]^[GA]AC KasI 788 GGCGCC G^GCGCC MaeI 226 CTAG C^TAG MflI 917 AGATCT [GA]^GATC[CT] NarI 788 GGCGCC GG^CGCC NheI 225 GCTAGC G^CTAGC NspI 1029 GCATGT [GA]CATG^[CT] PinAI 744 ACCGGT A^CCGGT PleI 82 GACTC GAGTC(4/5) PpuMI 690 GGGACCT [GA]G^G[AT]CC[CT] PstI 665 CTGCAG CTGCA^G SacI 358 GAGCTC GAGCT^C SapI 682 GCTCTTC GCTCTTC(1/4) SfcI 665 CTGCAG C^T[GA][CT]AG SfoI 788 GGCGCC GGC^GCC SgrAI 743 CACCGGTG C[GA]^CCGG[CT]G SmlI 892 CTCAAG C^T[CT][GA]AG TfiI 930 GAATC G^A[AT]TC XhoII 917 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 425 GACGCC G[GA]^CG[CT]C 788 GGCGCC AfaI 126 GTAC GT^AC 552 GTAC AlwNI 175 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 764 CAGCTCCTG BanI 741 GGCACC G^G[CT][GA]CC 788 GGCGCC BpmI 574 CTGGAG CTGGAG(16/14) 725 CTGGAG BsaHI 425 GACGCC G[GA]^CG[CT]C 788 GGCGCC BseRI 565 GAGGAG GAGGAG(10/8) 733 GAGGAG BsmAI 517 GAGAC GTCTC(1/5) 590 GAGAC Bsp1407I 125 TGTACA T^GTACA 551 TGTACA BsrDI 841 CATTGC GCAATG(2/0) 965 GCAATG BsrGI 125 TGTACA T^GTACA 551 TGTACA DpnII 879 GATC ^GATC 918 GATC EarI 683 CTCTTC CTCTTC(1/4) 616 GAAGAG HgaI 337 GACGC GACGC(5/10) 425 GACGC Hin1I 425 GACGCC G[GA]^CG[CT]C 788 GGCGCC HinfI 82 GACTC G^A[ACGT]TC 930 GAATC HphI 631 GGTGA GGTGA(8/7) 747 GGTGA Ksp632I 683 CTCTTC CTCTTC(1/4) 616 GAAGAG MboI 879 GATC ^GATC 918 GATC MboII 616 GAAGA GAAGA(8/7) 684 TCTTC MslI 141 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1020 CATACCTGTG NaeI 238 GCCGGC GCC^GGC 462 GCCGGC NdeII 879 GATC ^GATC 918 GATC NgoMIV 238 GCCGGC G^CCGGC 462 GCCGGC PflMI 430 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1062 CCAACAAATGG RsaI 126 GTAC GT^AC 552 GTAC Sau3AI 879 GATC ^GATC 918 GATC SfaNI 580 GCATC GCATC(5/9) 953 GCATC SmaI 60 CCCGGG CCC^GGG 467 CCCGGG SspBI 125 TGTACA T^GTACA 551 TGTACA Tsp509I 824 AATT ^AATT 836 AATT Van91I 430 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1062 CCAACAAATGG XmaCI 60 CCCGGG C^CCGGG 467 CCCGGG XmaI 60 CCCGGG C^CCGGG 467 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AluI ApaI AvaI BanII BbvI BcnI BmyI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TaqI TseI Tsp45I TspRI TthHB8I