(Circular) (Six-base) MAPSORT of: pFN21AE0612.seq Check: 6599 from: 1 to: 5794 pFN21AE0612 5794 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3857 5241 5509 1632 Size: 2225 1384 268 1917 Fragments arranged by size: 2225 1917 1384 268 AfeI AGC'GCT Cuts at: 2876 2876 Size: 5794 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 2749 4625 2749 Size: 1876 3918 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5794 AlwNI CAG_nnn'CTG Cuts at: 2185 2774 3537 5109 2185 Size: 589 763 1572 2870 Fragments arranged by size: 2870 1572 763 589 ApaI G_GGCC'C Cuts at: 2066 2358 2681 3088 2066 Size: 292 323 407 4772 Fragments arranged by size: 4772 407 323 292 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2840 3103 3329 3481 5590 1493 Size: 1347 263 226 152 2109 1697 Fragments arranged by size: 2109 1697 1347 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2414 2472 3119 1870 Size: 73 122 349 58 647 4545 Fragments arranged by size: 4545 647 349 122 73 58 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2746 2793 3115 4570 618 Size: 325 217 627 959 47 322 1455 1842 Fragments arranged by size: 1842 1455 959 627 325 322 217 47 BanII G_rGCy'C Cuts at: 1391 2066 2358 2367 2493 2630 2681 2689 2997 Size: 675 292 9 126 137 51 8 308 Cuts at: 2997 3088 3113 1391 Size: 91 25 4072 Fragments arranged by size: 4072 675 308 292 137 126 91 51 25 9 8 BbeI G_GCGC'C Cuts at: 2797 2797 Size: 5794 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4823 BbvCI CC'TCA_GC Cuts at: 2736 2736 Size: 5794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3985 4019 1903 Size: 34 50 34 1964 34 3678 Fragments arranged by size: 3678 1964 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2161 4405 136 Size: 107 122 71 631 605 489 2244 1525 Fragments arranged by size: 2244 1525 631 605 489 122 107 71 BglII A'GATC_T Cuts at: 2922 5789 2922 Size: 2867 2927 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 2066 2072 2292 2358 2681 2749 3088 3855 Size: 276 6 220 66 323 68 339 767 Cuts at: 3855 5011 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 767 339 323 276 220 68 66 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 4483 442 Size: 416 53 83 399 24 2108 958 1753 Fragments arranged by size: 2108 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2234 1056 Size: 1178 4616 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2751 4454 1239 Size: 1361 151 1703 2579 Fragments arranged by size: 2579 1703 1361 151 Bpu10I CC'TnA_GC Cuts at: 2629 2736 2861 2629 Size: 107 125 5562 Fragments arranged by size: 5562 125 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2882 3919 4784 5082 2882 Size: 1037 865 298 3594 Fragments arranged by size: 3594 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2589 4457 882 Size: 617 1090 1868 2219 Fragments arranged by size: 2219 1868 1090 617 BsaAI yAC'GTr Cuts at: 493 1204 3585 493 Size: 711 2381 2702 Fragments arranged by size: 2702 2381 711 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2431 2794 3601 3983 Size: 53 83 186 936 898 363 807 382 Cuts at: 3983 4618 275 Size: 635 1451 Fragments arranged by size: 1451 936 898 807 635 382 363 186 83 53 BsaWI w'CCGG_w Cuts at: 2749 4227 4625 4899 5046 5457 2749 Size: 1478 398 274 147 411 3086 Fragments arranged by size: 3086 1478 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5569 Fragments arranged by size: 5569 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2585 2753 1186 Size: 1399 168 4227 Fragments arranged by size: 4227 1399 168 BseYI C'CCAG_C Cuts at: 2275 2321 2599 4997 5493 2275 Size: 46 278 2398 496 2576 Fragments arranged by size: 2576 2398 496 278 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2933 1163 Size: 573 1197 4024 Fragments arranged by size: 4024 1197 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2165 2248 2428 3244 4005 5033 Size: 275 186 164 83 180 816 761 1028 Cuts at: 5033 1540 Size: 2301 Fragments arranged by size: 2301 1028 816 761 275 186 180 164 83 BsiHKAI G_wGCw'C Cuts at: 2367 3855 3940 5011 2367 Size: 1488 85 1071 3150 Fragments arranged by size: 3150 1488 1071 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2072 2292 2358 2367 2493 2630 Size: 399 276 6 220 66 9 126 137 Cuts at: 2630 2681 2689 2749 2997 3088 3113 3855 3940 Size: 51 8 60 248 91 25 742 85 Cuts at: 3940 5011 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 248 220 137 126 91 85 66 60 51 25 9 8 6 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 3683 5413 3683 Size: 1730 4064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2905 3149 844 Size: 2061 244 3489 Fragments arranged by size: 3489 2061 244 BsrBI CCG'CTC Cuts at: 1394 1823 2167 3681 1394 Size: 429 344 1514 3507 Fragments arranged by size: 3507 1514 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 2845 2977 4289 4463 65 Size: 1183 1597 132 1312 174 1396 Fragments arranged by size: 1597 1396 1312 1183 174 132 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 2160 2243 2467 2749 4438 4625 Size: 110 325 25 83 224 282 1689 187 Cuts at: 4625 1700 Size: 2869 Fragments arranged by size: 2869 1689 325 282 224 187 110 83 25 BsrGI T'GTAC_A Cuts at: 96 2130 2556 96 Size: 2034 426 3334 Fragments arranged by size: 3334 2034 426 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 2168 3854 4866 2168 Size: 1686 1012 3096 Fragments arranged by size: 3096 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5794 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstYI r'GATC_y Cuts at: 1972 2922 3124 3491 3876 3893 5345 5431 5443 Size: 950 202 367 385 17 1452 86 12 Cuts at: 5443 5789 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 385 367 346 202 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5242 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2070 2394 3310 4104 4124 2070 Size: 324 916 794 20 3740 Fragments arranged by size: 3740 916 794 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4801 809 Size: 507 3485 1802 Fragments arranged by size: 3485 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2162 2245 Size: 54 1425 50 275 18 289 43 83 Cuts at: 2245 2425 3241 4130 5759 8 Size: 180 816 889 1629 43 Fragments arranged by size: 1629 1425 889 816 289 275 180 83 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2162 2245 2425 3241 1537 Size: 275 350 83 180 816 4090 Fragments arranged by size: 4090 816 350 275 180 83 EarI CTCTTCn'nnn_ Cuts at: 1353 2616 2694 3724 1353 Size: 1263 78 1030 3423 Fragments arranged by size: 3423 1263 1030 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2493 4381 4755 4901 387 Size: 1064 138 904 1888 374 146 1280 Fragments arranged by size: 1888 1280 1064 904 374 146 138 EcoICRI GAG'CTC Cuts at: 2365 2365 Size: 5794 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 2751 3857 4454 5241 5509 1239 Size: 393 968 151 1106 597 787 268 1524 Fragments arranged by size: 1524 1106 968 787 597 393 268 151 EcoO109I rG'GnC_Cy Cuts at: 2062 2355 2677 2696 3084 2062 Size: 293 322 19 388 4772 Fragments arranged by size: 4772 388 322 293 19 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2031 2186 2306 2797 2878 4941 1678 Size: 353 155 120 491 81 2063 2531 Fragments arranged by size: 2531 2063 491 353 155 120 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4435 4467 4509 4541 1373 Size: 32 135 32 2863 32 42 32 2626 Fragments arranged by size: 2863 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3040 3138 3384 669 Size: 988 281 1102 98 246 3079 Fragments arranged by size: 3079 1102 988 281 246 98 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KasI G'GCGC_C Cuts at: 2793 2793 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4908 5092 1305 Size: 3603 184 2007 Fragments arranged by size: 3603 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3972 Fragments arranged by size: 3972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 3029 3752 4111 4270 518 Size: 648 984 879 723 359 159 2042 Fragments arranged by size: 2042 984 879 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2200 2207 2378 3210 3887 5035 5280 Size: 405 189 7 171 832 677 1148 245 Cuts at: 5280 1606 Size: 2120 Fragments arranged by size: 2120 1148 832 677 405 245 189 171 7 NaeI GCC'GGC Cuts at: 2245 2469 2245 Size: 224 5570 NarI GG'CG_CC Cuts at: 2794 2794 Size: 5794 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5242 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 2243 2467 2243 Size: 224 5570 NheI G'CTAG_C Cuts at: 1052 2230 1052 Size: 1178 4616 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 3038 3152 4697 1132 Size: 97 1809 114 1545 2229 Fragments arranged by size: 2229 1809 1545 114 97 PasI CC'CwG_GG Cuts at: 1348 2033 2681 1348 Size: 685 648 4461 Fragments arranged by size: 4461 685 648 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2441 3073 1108 Size: 1333 632 3829 Fragments arranged by size: 3829 1333 632 PfoI T'CCnGG_A Cuts at: 2658 2658 Size: 5794 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2968 3000 3863 3895 5376 5408 2968 Size: 32 863 32 1481 32 3354 Fragments arranged by size: 3354 1481 863 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2696 2696 Size: 5794 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2062 2354 2677 3084 2062 Size: 292 323 407 4772 Fragments arranged by size: 4772 407 323 292 PstI C_TGCA'G Cuts at: 830 2674 3146 830 Size: 1844 472 3478 Fragments arranged by size: 3478 1844 472 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 SacI G_AGCT'C Cuts at: 2367 2367 Size: 5794 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SapI GCTCTTCn'nnn_ Cuts at: 2694 2694 Size: 5794 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2670 3142 4277 4958 5149 826 Size: 219 1625 472 1135 681 191 1471 Fragments arranged by size: 1625 1471 1135 681 472 219 191 SfoI GGC'GCC Cuts at: 2795 2795 Size: 5794 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 2749 1700 Size: 1049 4745 SmaI CCC'GGG Cuts at: 1872 2067 2474 3121 1872 Size: 195 407 647 4545 Fragments arranged by size: 4545 647 407 195 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 3898 4799 5061 820 Size: 197 926 954 1001 901 262 1553 Fragments arranged by size: 1553 1001 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SrfI GCCC'GGGC Cuts at: 2067 2067 Size: 5794 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5794 StyI C'CwwG_G Cuts at: 513 1065 3233 513 Size: 552 2168 3074 Fragments arranged by size: 3074 2168 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3821 3838 3991 4176 4621 325 Size: 1255 2241 17 153 185 445 1498 Fragments arranged by size: 2241 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2556 4040 Size: 274 80 33 51 1443 153 426 1484 Cuts at: 4040 5752 96 Size: 1712 138 Fragments arranged by size: 1712 1484 1443 426 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5754 5786 5754 Size: 32 5762 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 2065 2472 3119 1870 Size: 195 407 647 4545 Fragments arranged by size: 4545 647 407 195 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SwaI XcmI