################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 202 GTCGAC GT^[AC][GT]AC AlwI 678 GATCC GGATC(4/5) AseI 577 ATTAAT AT^TAAT AsnI 577 ATTAAT AT^TAAT Asp700I 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 505 GGACC G^G[AT]CC BalI 368 TGGCCA TGG^CCA BciVI 567 GGATAC GTATCC(6/5) BfaI 475 CTAG C^TAG BpmI 153 CTCCAG CTGGAG(16/14) BsaAI 364 TACGTG [CT]AC^GT[GA] BsiEI 197 CGGTCG CG[GA][CT]^CG BsmAI 332 GTCTC GTCTC(1/5) BsmFI 124 GGGAC GGGAC(10/14) BspHI 401 TCATGA T^CATGA BstYI 677 AGATCC [GA]^GATC[CT] DdeI 871 CTTAG C^T[ACGT]AG EaeI 368 TGGCCA [CT]^GGCC[GA] HincII 202 GTCGAC GT[CT]^[GA]AC HindII 202 GTCGAC GT[CT]^[GA]AC MaeI 475 CTAG C^TAG MaeII 365 ACGT A^CGT MaeIII 480 GTTAC ^GT[ACGT]AC MflI 677 AGATCC [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MwoI 231 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 577 ATTAAT AT^TAAT RcaI 401 TCATGA T^CATGA SalI 202 GTCGAC G^TCGAC SapI 84 GCTCTTC GCTCTTC(1/4) TaqI 203 TCGA T^CGA TfiI 63 GATTC G^A[AT]TC TthHB8I 203 TCGA T^CGA Van91I 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 677 AGATCC [GA]^GATC[CT] XmnI 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 196 GCGG CCGC(-3/-1) 565 GCGG AlwNI 235 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 605 CAGTGTCTG BanI 228 GGTGCC G^G[CT][GA]CC 695 GGTGCC BbvI 221 GCAGC GCAGC(8/12) 284 GCTGC BstF5I 256 CATCC GGATG(2/0) 905 GGATG Cac8I 217 GCTTGC GC[ACGT]^[ACGT]GC 280 GCTGGC DpnII 358 GATC ^GATC 678 GATC Fnu4HI 221 GCAGC GC^[ACGT]GC 284 GCTGC FokI 256 CATCC GGATG(9/13) 905 GGATG HphI 243 GGTGA GGTGA(8/7) 345 GGTGA ItaI 221 GCAGC GC^[ACGT]GC 284 GCTGC MboI 358 GATC ^GATC 678 GATC NdeII 358 GATC ^GATC 678 GATC Sau3AI 358 GATC ^GATC 678 GATC TseI 221 GCAGC G^C[AT]GC 284 GCTGC Tsp509I 537 AATT ^AATT 861 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI BanII BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cfr13I EarI EcoRII EcoT14I HaeIII HinfI Ksp632I MboII MnlI MseI MslI MvaI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI StyI Tru9I TspRI